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We narrowed to 150,160 results for: ins

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Showing: 5681 - 5700 of 150160 results

  1. pC0049-EF1a-dPSPCas13b-NES-HIV, H133A/H1058A

    Plasmid
    #103865
    Purpose
    Catalytically inactive PSPCas13b. RNase activity is inactivated by mutation of catalytic histidine residues in the HEPN domains (H133A/H1058A).
    Depositor
    Feng Zhang
    Article
    Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi
    Insert
    PspCas13b
    Use
    CRISPR and Lentiviral
    Tags
    3xHA and HIV NES
    Expression
    Mammalian
    Mutation
    Contains the H133A and H1058A mutations which ina…
    Promoter
    EF1a
    Available Since
    Jan. 4, 2018
    Availability
    Academic Institutions and Nonprofits only
    103865-plasmid-map-sequence-id-243540

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  4. pCMV-2E2-HA-mCh

    Plasmid
    #129596
    Purpose
    The encoded protein is the anti-HA frankenbody variant fused with the mCherry. It can be used to track mature and nascent HA tagged proteins in living organism.
    Depositor
    Tim Stasevich
    Article
    Zhao et al Nat Commun. 2019 Jul 3;10(1):2947. doi:
    Insert
    Anti-HA frankenbody variant-mCherry (2E2-HA scFv-mCherry)
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Aug. 27, 2019
    Availability
    Industry, Academic Institutions, and Nonprofits
    129596-plasmid-map-sequence-id-255026

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  5. pRSF-G1TFAKRS

    Plasmid
    #177311
    Purpose
    tRNA synthetase/tRNA pair for the in vivo incorporation of N6-(trifluoroacetyl)-L-lysine (TFA-Lys) into proteins in E. coli in response to the amber (TAG) codon
    Depositor
    Thomas Huber
    Article
    Orton et al J Am Chem Soc. 2021 Nov 15. doi: 10.1021
    Insert
    tRNA synthetase
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Nov. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    177311-plasmid-map-sequence-id-348119

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  6. MSP1830

    Plasmid
    #70708
    Purpose
    Human expression plasmid for SaCas9 KKH variant: CAG-humanSaCas9(E782K/N968K/R1015H)-NLS-3xFLAG
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038
    Insert
    mammalian codon-optimized KKH variant Staphylococcus aureus Cas9
    Use
    CRISPR
    Tags
    NLS-3xFLAG
    Expression
    Mammalian
    Mutation
    E782K, N968K and R1015H in SaCas9
    Promoter
    CAG
    Available Since
    Nov. 6, 2015
    Availability
    Academic Institutions and Nonprofits only
    70708-plasmid-map-sequence-id-364684

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  9. BPK764

    Plasmid
    #65767
    Purpose
    Bacterial expression plasmid for SpCas9 & sgRNA (need to clone spacer into BsaI sites): T7-humanSpCas9-NLS-3xFLAG-T7-BsaIcassette-Sp-sgRNA
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature
    Insert
    mammalian codon-optimized Streptococcus pyogenes Cas9-NLS-3XFlag, and SpCas9 gRNA
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Bacterial
    Promoter
    T7 (x2)
    Available Since
    June 24, 2015
    Availability
    Academic Institutions and Nonprofits only
    65767-plasmid-map-sequence-id-471631

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  12. HIV-1 LTR-gfp

    Plasmid
    #115809
    Purpose
    HIV-1 LTR driven reporter vector that retains complete LTRs, tat, and rev, but has a frameshift mutation in env, an ngfr reporter gene in place of nef, and gfp in place of gag, pol, vif, and vpr
    Depositor
    Jeremy Luban
    Article
    Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.
    Insert
    gfp and ngfr
    Use
    Lentiviral
    Promoter
    HIV1 LTR
    Available Since
    Feb. 14, 2019
    Availability
    Academic Institutions and Nonprofits only
    115809-plasmid-map-sequence-id-232377

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  18. pDV06_feedback_entry_TagBFP-2A-MCS-2A-mNeonGreen-2A-MCP-ADAR2dd(E488Q)-NES

    Plasmid
    #200402
    Purpose
    Entry vector for closed-loop ADAR-based RNA sensors (DART VADAR). Expresses TagBFP constitutively. Expresses mNeonGreen and MCP-ADAR upon target detection
    Depositor
    James Collins
    Article
    Gayet et al Nat Commun. 2023 Mar 11;14(1):1339. doi:
    Insert
    Closed-loop DART VADAR sensor entry cassette
    Use
    Synthetic Biology
    Expression
    Mammalian
    Available Since
    June 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    200402-plasmid-map-sequence-id-396788

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Showing: 5681 - 5700 of 150160 results