We narrowed to 11,743 results for: pcdna

1. pcDNA3.1(+) ZKSCAN1 MCS-WT Split GFP

   Plasmid

   #69908

   Purpose

   Expresses a GFP circular RNA in mammalian cells

   Depositor

   Jeremy Wilusz

   Article

   Kramer et al Genes Dev. 2015 Oct 8.

   Insert

   ZKSCAN1, GFP (ZKSCAN1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCDNA3-Myc3-Cul3 delta N41

   Plasmid

   #21590

   Depositor

   Yue Xiong

   Article

   Furukawa et al Mol Cell Biol. 2005 Jan . 25(1):162-71.

   Insert

   cullin 3 (CUL3 Human)
   

   Use

   Tags

   myc3

   Expression

   Mammalian

   Mutation

   Residues 34-74 deleted from N terminus.

   Promoter

   Available since

   July 1, 2009

   Availability

   Academic Institutions and Nonprofits only

3. pcDNA3.1 2xFLAG-2xSTREP RAD51 (F129A)

   Plasmid

   #158952

   Purpose

   Express human RAD51(F129A) mutant in mammalian cells

   Depositor

   Michele Pagano

   Article

   Marzio et al Mol Cell. 2019 Jan 17;73(2):224-237.e6.

   Insert

   RAD51 (RAD51 Human)
   

   Use

   Tags

   2X FLAG 2X STREP

   Expression

   Mammalian

   Mutation

   changed phenylalanine 129 to alanine

   Promoter

   CMV

   Available since

   Sept. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA-GalNAc-T2-GFP-ESCargo(FTV)

   Plasmid

   #140163

   Purpose

   Expresses a regulatable secretory cargo for mammalian cells and a Golgi marker

   Depositor

   Benjamin Glick

   Article

   Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

   Insert

   GalNAc-T2-msGFP2 and piGH-FTVNTT-DsRed-Express2-FKBP(LV)(C22V) (GALNT2 Synthetic, Human)
   

   Use

   Tags

   ER signal sequence (MGWSCIILFLVATATGAHS) (N termi…

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a

   Available since

   March 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3-K27 histone methylation reporter

   Plasmid

   #22865

   Depositor

   Alice Ting

   Article

   Lin et al J Am Chem Soc. 2004 May 19. 126(19):5982

   Insert

   K27 HMR
   

   Use

   Tags

   CFP and YFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 22, 2010

   Availability

   Academic Institutions and Nonprofits only

6. 1013 pcDNA3 T7 Akt1 T308A S473A

   Plasmid

   #9030

   Depositor

   William Sellers

   Article

   Ramaswamy et al Proc Natl Acad Sci U S A. 1999 Mar 2. 96

   Insert

   Akt1 T308A S473A (AKT1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   T308A S473A

   Promoter

   Available since

   Jan. 5, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3-Au1-mTOR-Wild type

   Plasmid

   #26036

   Depositor

   Fuyuhiko Tamanoi

   Article

   Sato et al Oncogene. 2010 May 6. 29(18):2746-52.

   Insert

   mammalian target of rapamycin-wild type (Mtor Rat)
   

   Use

   Tags

   AU1

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 21, 2010

   Availability

   Academic Institutions and Nonprofits only

8. pcDNA3.1-2xFLAG-2xSTREP (empty vector)

   Plasmid

   #172604

   Purpose

   Empty vector for the cloning of an N-terminal 2xFLAG-2xSTREP-tagged gene of interest, and expression in mammalian cells

   Depositor

   Michele Pagano

   Article

   Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

   Type

   Empty backbone

   Use

   Tags

   2xFLAG-2xSTREP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA3-3myc-6His-EZH2 21D

   Plasmid

   #42664

   Depositor

   Mien-Chie Hung

   Article

   Cha et al Science. 2005 Oct 14;310(5746):306-10.

   Insert

   EZH2 (EZH2 Human)
   

   Use

   Tags

   3Myc and 6His

   Expression

   Mammalian

   Mutation

   S21D

   Promoter

   Available since

   July 17, 2013

   Availability

   Academic Institutions and Nonprofits only

10. CoxVIIIx2-OeNL(Ca2+)_18μ-pcDNA3

    Plasmid

    #111924

    Purpose

    Orange color luminescent indicator for calcium signaling.Mitochondrial targeting signals of subunit VIII of human cytochrome c oxidase (CoxVIII) were fused at the N-terminus of OeNL(Ca2+)_18μ

    Depositor

    Takeharu Nagai

    Article

    Hossain et al ACS Chem Biol. 2018 Mar 9. doi: 10.1021/

    Insert

    OeNL(Ca2+)_18μ
    

    Use

    Tags

    CoxVIIIx2

    Expression

    Mammalian

    Mutation

    E67D, E104D, D133E at CaM

    Promoter

    CMV

    Available since

    June 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1(+) ZKSCAN1 MCS-ciRS7 Exon

    Plasmid

    #69906

    Purpose

    Expresses the human ciRS7/CDR1as circular RNA in mammalian cells

    Depositor

    Jeremy Wilusz

    Article

    Kramer et al Genes Dev. 2015 Oct 8.

    Insert

    ciRS7 (CDR1-AS Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA3.1 CARD8-TEV-GFP-FLAG

    Plasmid

    #169991

    Purpose

    Mammalian purification construct for CARD8

    Depositor

    Daniel Bachovchin

    Article

    Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

    Insert

    CARD8 (CARD8 Human)
    

    Use

    Tags

    TEV-GFP-FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA5/FRT/TO-Venus-Flag (865)

    Plasmid

    #40998

    Depositor

    Jonathon Pines

    Article

    Sedgwick et al EMBO J. 2013 Jan 23;32(2):303-14. doi: 1

    Type

    Empty backbone

    Use

    Tags

    3xFLAG (DYKDHDGDYKDHDIDYKDDDDK) and Venus YFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 8, 2012

    Availability

    Academic Institutions and Nonprofits only

14. pcDNA3.1-CAG-sbGLuc-hGtARC2-EYFP

    Plasmid

    #114111

    Purpose

    fusion protein of Gaussia luciferase variant slow burn, Guillardia theta anion channelrhodopsin-2, and enhanced yellow fluorescent protein for bioluminescent optogenetics

    Depositor

    Ute Hochgeschwender

    Article

    Prakash et al J Neurosci Res. 2018 Aug 28. doi: 10.100

    Insert

    Gaussia luciferase, slow burn; Guillardia theta anion channelrhodopsin-2, human codon optimized
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Aug. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA5 FRT TO Myc ArA K162R

    Plasmid

    #59805

    Purpose

    Allows the integration of Myc ArA K162R in the genome and Tet-inducible expression

    Depositor

    Stephen Taylor

    Article

    Girdler et al J Cell Sci. 2006 Sep 1;119(Pt 17):3664-7

    Insert

    Aurora A (AURKA Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    K162R, kinase mutant

    Promoter

    hybrid human cytomegalovirus (CMV)/TetO2 promoter

    Available since

    Feb. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1 LIC 6A FLAG-TEV-DPP9S

    Plasmid

    #166794

    Purpose

    Mammalian cell expression vector for Flag-tagged DPP9S

    Depositor

    Hao Wu

    Article

    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

    Insert

    DPP9 (DPP9 Human)
    

    Use

    Tags

    FLAG-TEV

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. Orange Nano-lantern(Ca2+)/pcDNA3

    Plasmid

    #71346

    Purpose

    Express Orange Nano-lantern-based luminescent Ca2+ indicator in mammalian cell

    Depositor

    Takeharu Nagai

    Article

    Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

    Insert

    Orange Nano-lantern(Ca2+)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

18. pcDNA5 FRT-Hygro hTERT FL-Firefly

    Plasmid

    #171057

    Purpose

    hTERT Full-length-expressing splicing minigene

    Depositor

    Agnel Sfeir

    Article

    Penev et al Mol Cell. 2021 Apr 6. pii: S1097-2765(21

    Insert

    hTERT FL-Firefly
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA5 FRT/TO SF-HERC2 C4762S (ShB-R)

    Plasmid

    #55614

    Purpose

    Dox-inducible expression of full length, ShB-resistant, 3xFLAG/STREP tagged C4762S (catalytically inactive) HERC2 in Flp-In T-Rex cells

    Depositor

    David Chan

    Article

    Chan et al J Biol Chem. 2014 May 22. pii: jbc.M114.

    Insert

    3xFLAG tagged HERC2 (C4762S) (HERC2 Human)
    

    Use

    Tags

    3xFLAG and STREP

    Expression

    Mammalian

    Mutation

    C4762 mutated to serine to render HERC2 catalytic…

    Promoter

    Available since

    Sept. 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3-3myc-6His-EZH2 21A

    Plasmid

    #42663

    Depositor

    Mien-Chie Hung

    Article

    Cha et al Science. 2005 Oct 14;310(5746):306-10.

    Insert

    EZH2 (EZH2 Human)
    

    Use

    Tags

    3Myc and 6His

    Expression

    Mammalian

    Mutation

    S21A

    Promoter

    Available since

    July 17, 2013

    Availability

    Academic Institutions and Nonprofits only