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We narrowed to 20 results for: pcdna

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Showing: 1 - 20 of 20 results

  1. Twenty Years of Sharing Science!

    Type
    Blog Post
    ...Our first request was on September 8, 2004 (for pcDNA-p160MBP, if you're curious.) Since then, we've distributed...

  3. Choosing Your Perfect Empty Backbone

    Type
    Blog Post
    ...mammalian expression vectors, c-Flag pcDNA3, comes from the pcDNA series. It has the CMV promoter for ...protein will allow you to visualize YGOI in vivo (try pcDNA3-EGFP). If you can get away with generating transiently...

  4. Addgene: The First Twenty Years

    Type
    Blog Post
    ... plasmid request came on September 8, 2004, for pcDNA-p160MBP, while the first request for a kit came ...

  5. CRISPR 101: Epigenetics and Editing the Epigenome

    Type
    Blog Post
    ...mammalian expression constructs including pcDNA-dCas9-p300 Core and pcDNA3.3-Nm-dCas9-p300 Core, as well as pLV-dCas9... in mammalian cells. This plasmid is used with pcDNA3.1-MS2-Tet1-CD to decrease methylation and activate...Methyltransferase MQ1 Margaret Goodell's lab has deposited pcDNA3.1-dCas9-MQ1(Q147L)-EGFP, a fusion of dCas9 to a ...

  6. Fluorescent Protein Guide: In Vivo Imaging

    Type
    Collection
    ...643 670 12.7 piRFP670-N1 miRFP670nano3 645 670 24 pcDNA-miRFP670nano3 miRFP680 661 680 14 pmiRFP680-N1 iRFP682...Plasmids mCardinal 604 659 18 pcDNA3-mCardinal mNeptune2 600 650 21 pcDNA3-mNeptune2 mNeptune2-N1 E2-Crimson...

  7. TALEN Plasmids and Kits

    Type
    Collection
    ...rate of Golden Gate assembly. Destination vectors, pcDNA-TAL-NC2 and pCAGGS-TAL-NC2, are mammalian expression...in cultured cells. Golden Gate TALEN 2.0 47388 pcDNA3.1-GoldenGate Charles Gersbach These new destination...activation domain of VP16) at the C terminus. 47389 pcDNA3.1-GoldenGate-VP64 Golden Gate TALEN 2.0 47874 pTALYM3...

  8. Luciferase Plasmid Collection

    Type
    Collection
    ...Nano-lantern/pcDNA3 Nano-lantern CMV Mammalian expression of Nano-lantern Takeharu Nagai 87121 pcDNA-RLuc8 Renilla...upstream of IRES-firefly luciferase. Bryan Welm 87067 pcDNA3.1-ccdB-Nanoluc NanoLuc® Creation of N-terminal Nanoluc...Renilla luciferase is co-expressed. Oskar Laur 87070 pcDNA3.1-Nanoluc-ccdB NanoLuc® Creation of N-terminal Nanoluc...firefly luciferase Eric Campeau 18964 Luciferase-pcDNA3 Firefly CMV Mammalian expression of firefly luciferase...expression of firefly luciferase Javier Alcudia 113442 pcDNA3.1 NL NanoLuc® CMV Mammalian expression of Nanoluc...

  9. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ...Takashi Yamamoto pcDNA-TAL-NC2, pCAGGS-TAL-NC2 Charles Gersbach pcDNA3.1-GoldenGate, pcDNA3.1-GoldenGate-...Type 74309 pcDNA3.0-TRPV4-p2A-ferritin-p2A-mCherry Mammalian Expression 74308 pcDNA3.0-Magneto2.0-p2A-mCherry...

  10. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ...Expression miRFP670nano3 645 670 24 4.2 Monomer pcDNA-miRFP670nano3 - Mammalian Expression pAAV-hSYN-miRFP670nano3...Expression ECFP 433 475 13 4.7 Prone to dimerization pcDNA3-CFP - Mammalian Expression pCAG-CFP - Mammalian... EGFP 488 507 34 6 25 min Prone to dimerization pcDNA3-EGFP - Mammalian Expression pCAG-GFP - Mammalian...(StayGold-E138D) 497 504 126 4.6 14 min Monomer pcDNA3-10His-mStayGold (E138D) - Mammalian Expression ...mGreenLantern 503 514 73 5.6 14 min Monomer (A206K) pcDNA3.1-mGreenLantern - Mammalian Expression pAAV-CAG-...Clover 505 515 84 6.1 30 min Prone to dimerization pcDNA3-Clover - Mammalian Expression pFA6a-link-yoClover-Kan...Plasmids EYFP 513 527 51 6.9 Prone to dimerization pcDNA3-YFP - Mammalian Expression Topaz 514 527 57 Prone...

  11. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...Cookson 13315 pcDNA-DEST53 PINK1 N-GFP PINK1 GFP CMV Parkinson's Mark Cookson 13316 pcDNA-DEST47 PINK1 ...Aaron Gitler 29609 pcDNA 3.2-FUS-1-526aa-V5 FUS V5 CMV ALS Aaron Gitler 29610 pcDNA 3.2-FUS-1-269aa-V5...Aaron Gitler 29611 pcDNA 3.2-FUS-1-373aa-V5 FUS V5 CMV ALS Aaron Gitler 29612 pcDNA 3.2-FUS-1-413aa-V5...Lindquist 14121 pcDNA3-HtrA2-EGFP HTRA2 GFP CMV Parkinson's L. Miguel Martins 14122 pcDNA3-HtrA2-EGFP S306A...Mombaerts 15938 pcDNA3-HtrA2-FLAG HTRA2 Flag CMV Parkinson's L. Miguel Martins 15939 pcDNA3-HtrA2-FLAG S142D...Cliff Tabin 19457 pcDNA5/FRT/TO HSPA1L HSPA1L CMV Parkinson's Harm Kampinga 19484 pcDNA5/FRT/TO GFP HSPA1L...Kampinga 19511 pcDNA5/FRT/TO V5 HSPA1L HSPA1L V5 CMV Parkinson's Harm Kampinga 19538 pcDNA5/FRT/TO HIS HSPA1L...

  16. Arf GTPase Family

    Type
    Collection
    ...pET), Mammalian (pcDNA3), Gateway GTPase Arf3 377 181 Bacterial (pET), Mammalian (pcDNA3), Gateway GTPase...pET), Mammalian (pcDNA3), Gateway GTPase Arf5 381 180 Bacterial (pET), Mammalian (pcDNA3), Gateway GTPase...pET), Mammalian (pcDNA3), Gateway GTPase Arl3 403 182 Bacterial (pET), Mammalian (pcDNA3), Gateway GTPase...(pET), Mammalian (pcDNA3) GTPase Arfrp1 10139 201 Bacterial (pET), Mammalian (pcDNA3), Gateway GTPase ... (pET), Mammalian (pcDNA3) GAP Arfgap2 84364 521 Bacterial (pET), Mammalian (pcDNA3) GAP Arfgap3 26286...pGEX4T), Mammalian (pcDNA3) GAP ELMOD2 255550 293 Bacterial (pGEX4T), Mammalian (pcDNA3) GAP ELMOD3 84173...GTPase Arf6 382 175 Bacterial (pET), Mammalian (pcDNA3), Gateway GTPase Arl1 400 181 Bacterial (pET), Mammalian...

  17. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ... Backbones Flag Epitope tag c-Flag pcDNA3 or FLAG-HA-pcDNA3.1 - C-terminal Flag or N-terminal FLAG-...C-terminus of BioID2 3xHA-TurboID-NLS_pCDNA3 or 3xHA-miniTurbo-NLS_pCDNA3 - Expresses 3xHA-tagged TurboID...expression plasmid for cloning untagged proteins pcDNA3.1-mCherry - Expresses mCherry tag in mammalian cells... including Xenopus HA Epitope tag pcDNA3.1-HA - Mammalian expression vector with N-terminal...Fluorescent proteins (GFP, mCherry, etc) Localization pcDNA3-EGFP - C-terminal GFP for mammalian expression ...plasmid without effector fusions; 3X Flag tag; 2NLS; pCDNA3 vector backbone, mammalian expression pENTR4-myc-nuc...plasmids with Neomycin selection Puromycin Mammalian pcDNA3.1-(-)-Pur - Eukaryotic expression vector Tet-pLKO-puro...

  18. Fluorescent Proteins: FRET

    Type
    Collection
    ...mCherry2-N1 , pcDNA3.1 CMV mCherry-eGFP BGH Clover mRuby2 505 0.76 600 113,000 0.38 6.3 4.5 pcDNA3-Clover ,... , pcDNA3-mRuby2 , pcDNA3.1-Clover-mRuby2 mClover3 mRuby3 506 0.78 592 128,000 0.45 6.4 4.9 pNCS-mClover3...0.45 6.4 4.7 pCS2+mNeonGreen-C Cloning Vector , pcDNA3-mRuby2 , mNeonGreen-mRuby2-FRET-10 SYFP2 mScarlet3...

  19. Fluorescent Protein Guide: Subcellular Localization

    Type
    Collection
    ...filaments LifeAct mGreenLantern Gregory Petsko 212020 pcDNA3/mStayGold(c4)=UtrCH Actin filaments Utrophin calponin...homology domain mStayGold Atsushi Miyawaki 212019 pcDNA3/F-tractin=mStayGold Actin filaments F-tractin mStayGold... LC3 EGFP Karla Kirkegaard 40827 mCherry-hLC3B-pcDNA3.1 Autophagosomes LC3 mCherry David Rubinsztein 38277...apparatus Giantin mNeonGreen Dorus Gadella 212022 pcDNA3/td5StayGold(c4)=GianCreg Golgi apparatus Giantin...

  20. Genetic Code Expansion

    Type
    Collection
    ...L-lysine (TFA-Lys) Bacterial TAG Thomas Huber 182287 pcDNA3.1(+)_U6 tRNAPyl_CMV NESPylRS(AF) Y306A/Y384F (AF...PylRS M. mazei Bacterial TAA Wenshe Liu 182652 pcDNA3.1(+)_4x(U6 tRNA M15)_CMV NESPylRS(AF) PylRS M. mazei...mazei Mammalian TAG Ivana Nikić-Spiegel 182653 pcDNA3.1(+)_U6 tRNAPyl_CMV NESPylRS(AF)_IRES_eRF1(E55D)...
Showing: 1 - 20 of 20 results