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  1. Twenty Years of Sharing Science!

    Type
    Blog Post
    ...Our first request was on September 8, 2004 (for pcDNA-p160MBP, if you're curious.) Since then, we've distributed...

  3. Choosing Your Perfect Empty Backbone

    Type
    Blog Post
    ...mammalian expression vectors, c-Flag pcDNA3, comes from the pcDNA series. It has the CMV promoter for ...protein will allow you to visualize YGOI in vivo (try pcDNA3-EGFP). If you can get away with generating transiently...

  4. Addgene: The First Twenty Years

    Type
    Blog Post
    ... plasmid request came on September 8, 2004, for pcDNA-p160MBP, while the first request for a kit came ...

  5. CRISPR 101: Epigenetics and Editing the Epigenome

    Type
    Blog Post
    ...mammalian expression constructs including pcDNA-dCas9-p300 Core and pcDNA3.3-Nm-dCas9-p300 Core, as well as pLV-dCas9... in mammalian cells. This plasmid is used with pcDNA3.1-MS2-Tet1-CD to decrease methylation and activate...Methyltransferase MQ1 Margaret Goodell's lab has deposited pcDNA3.1-dCas9-MQ1(Q147L)-EGFP, a fusion of dCas9 to a ...

  6. TALEN Plasmids and Kits

    Type
    Collection
    ...rate of Golden Gate assembly. Destination vectors, pcDNA-TAL-NC2 and pCAGGS-TAL-NC2, are mammalian expression...in cultured cells. Golden Gate TALEN 2.0 47388 pcDNA3.1-GoldenGate Charles Gersbach These new destination...activation domain of VP16) at the C terminus. 47389 pcDNA3.1-GoldenGate-VP64 Golden Gate TALEN 2.0 47874 pTALYM3...

  7. Luciferase Plasmid Collection

    Type
    Collection
    ...Nano-lantern/pcDNA3 Nano-lantern CMV Mammalian expression of Nano-lantern Takeharu Nagai 87121 pcDNA-RLuc8 Renilla... processes, biosensors, or optogenetic tools . pcDNA3.1+-pHlash , pRSETb-pHlash : A pH sensor created ...Gateway cloning. Lentiviral. Jorge Ferrer 87067 pcDNA3.1-ccdB-Nanoluc NanoLuc® Creation of N-terminal Nanoluc...Renilla luciferase is co-expressed. Oskar Laur 87070 pcDNA3.1-Nanoluc-ccdB NanoLuc® Creation of N-terminal Nanoluc...fusions using Gateway cloning Mikko Taipale 87065 pcDNA3.1-ccdB-Renilla Renilla Creation of C-terminal Renilla...fusions using Gateway cloning Mikko Taipale 87066 pcDNA3.1-ccdB-Firefly Firefly Creation of C-terminal Firefly...fusions using Gateway cloning Mikko Taipale 87068 pcDNA3.1-Renilla-ccdB Renilla Creation of N-terminal Renilla...

  8. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ...Takashi Yamamoto pcDNA-TAL-NC2, pCAGGS-TAL-NC2 Charles Gersbach pcDNA3.1-GoldenGate, pcDNA3.1-GoldenGate-...Type 74309 pcDNA3.0-TRPV4-p2A-ferritin-p2A-mCherry Mammalian Expression 74308 pcDNA3.0-Magneto2.0-p2A-mCherry...

  9. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...Cookson 13315 pcDNA-DEST53 PINK1 N-GFP PINK1 GFP CMV Parkinson's Mark Cookson 13316 pcDNA-DEST47 PINK1 ...Aaron Gitler 29609 pcDNA 3.2-FUS-1-526aa-V5 FUS V5 CMV ALS Aaron Gitler 29610 pcDNA 3.2-FUS-1-269aa-V5...Aaron Gitler 29611 pcDNA 3.2-FUS-1-373aa-V5 FUS V5 CMV ALS Aaron Gitler 29612 pcDNA 3.2-FUS-1-413aa-V5...Lindquist 14121 pcDNA3-HtrA2-EGFP HTRA2 GFP CMV Parkinson's L. Miguel Martins 14122 pcDNA3-HtrA2-EGFP S306A...Mombaerts 15938 pcDNA3-HtrA2-FLAG HTRA2 Flag CMV Parkinson's L. Miguel Martins 15939 pcDNA3-HtrA2-FLAG S142D...Cliff Tabin 19457 pcDNA5/FRT/TO HSPA1L HSPA1L CMV Parkinson's Harm Kampinga 19484 pcDNA5/FRT/TO GFP HSPA1L...Kampinga 19511 pcDNA5/FRT/TO V5 HSPA1L HSPA1L V5 CMV Parkinson's Harm Kampinga 19538 pcDNA5/FRT/TO HIS HSPA1L...

  13. Arf GTPase Family

    Type
    Collection
    ...pET), Mammalian (pcDNA3), Gateway GTPase Arf3 377 181 Bacterial (pET), Mammalian (pcDNA3), Gateway GTPase...pET), Mammalian (pcDNA3), Gateway GTPase Arf5 381 180 Bacterial (pET), Mammalian (pcDNA3), Gateway GTPase...pET), Mammalian (pcDNA3), Gateway GTPase Arl3 403 182 Bacterial (pET), Mammalian (pcDNA3), Gateway GTPase... (pET), Mammalian (pcDNA3) GAP Arfgap2 84364 521 Bacterial (pET), Mammalian (pcDNA3) GAP Arfgap3 26286...Mammalian (pcDNA3, pEGFP) GAP Git2 9815 759 GAP ELMOD1 55531 334 Bacterial (pGEX4T), Mammalian (pcDNA3) GAP ...pGEX4T), Mammalian (pcDNA3) GAP ELMOD3 84173 381 Bacterial (pGEX4T), Mammalian (pcDNA3) GEF Arfgef1 10565...GTPase Arf6 382 175 Bacterial (pET), Mammalian (pcDNA3), Gateway GTPase Arl1 400 181 Bacterial (pET), Mammalian...

  14. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ...Expression ECFP 433 475 13 4.7 Prone to dimerization pcDNA3-CFP - Mammalian Expression pCAG-CFP - Mammalian...Expression EGFP 488 507 34 6 Prone to dimerization pcDNA3-EGFP - Mammalian Expression pCAG-GFP - Mammalian...Clover 505 515 84 6.1 30 min Prone to dimerization pcDNA3-Clover - Mammalian Expression pFA6a-link-yoClover-Kan...Plasmids EYFP 513 527 51 6.9 Prone to dimerization pcDNA3-YFP - Mammalian Expression Topaz 514 527 57 Prone...Expression mCitrine 516 529 59 5.7 Monomer (A206K) pcDNA3 mCitrine LIC cloning vector (6C) - Mammalian Expression...mRuby2 559 600 43 5.3 150 min Prone to dimerization pcDNA3-mRuby2 - Mammalian Expression pFA6a-link-yomRuby2...Bacterial Expression mRFP1 584 607 13 4.5 1 hr Monomer pcDNA3-mRFP - Mammalian Expression pMXs-mRFP1 - Mammalian...

  15. Fluorescent Protein Guide: Subcellular Localization

    Type
    Collection
    ...Extracellular milieu cfSGFP2-N EGFP Ikuo Wada 73208 pcDNA3.1-COXIV-COX8-dL5-2XG4S-mCer3 Mitochondria COX IV-derived...Nuc Nucleus NLS mKalama1 Robert Campbell 73205 pcDNA3.1-NLS-myc-dL5-2xG4S-mCer3 Nucleus NLS (from Mak16p...protein) dL5 FAP; mCerulean3 Marcel Bruchez 73207 pcDNA3.1-KozATG-dL5-2XG4S-mCer3 Cytosol, nucleoplasm None...sequence from p63 mTurquoise2 Dorus Gadella 73206 pcDNA3.1-kappa-myc-dL5-2xG4S-TMst Cell surface (mammalian... Peroxisome SKL mNeonGreen Dorus Gadella 73210 pcDNA3.1-KozATG-myc-dL5-2XG4S-mCer3-SKL Peroxisome SKL ...LC3 mCherry, GFP Anne Brunet 40827 mCherry-hLC3B-pcDNA3.1 Autophagosome LC3 mCherry David Rubinsztein 11546...Endoplasmic Reticulum Sec61 BFP Gia Voeltz 73209 pcDNA3.1-kappa-myc-dL5-2XG4S-mCer3-KDEL Endoplasmic Reticulum...

  16. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ...Localization pcDNA3-EGFP - C-terminal GFP for mammalian expression pcDNA3 GFP LIC cloning...Representative Empty Backbones Flag Epitope tag pcDNA3 Flag HA - N-terminal Flag-HA for...C-terminal nuclear localization signal FNpcDNA3 - N-terminal FLAG and SV40 nuclear...

  17. Cre-lox system

    Type
    Collection
    ...122960 pcDNA3.1_PA-Cre photo-activatable Cre recombinase CMV Mammalian Sato 122961 pcDNA3.1_PA-Cre-Y324F...Binding Domain (EBD) Tdh4 Yeast van Leeuwen 65727 pcDNA3.1-CMV-CFP;UBC-Cre25nt Cre with a 25 nucleotide extracellular...

  18. Genetic Code Expansion

    Type
    Collection
    ...L-lysine (TFA-Lys) Bacterial TAG Thomas Huber 182287 pcDNA3.1(+)_U6 tRNAPyl_CMV NESPylRS(AF) Y306A/Y384F (AF...PylRS M. mazei Bacterial TAA Wenshe Liu 182653 pcDNA3.1(+)_U6 tRNAPyl_CMV NESPylRS(AF)_IRES_eRF1(E55D)...

  20. Fujii Lab CRISPR Plasmids

    Type
    Collection
    ...human IRF-1 promoter. 98041 Sa-dCas9-NLS-3xFLAG/pcDNA3.1 Expresses Sa-dCas9-NLS-3xFLAG in mammalian cells...
Showing: 1 - 20 of 20 results