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Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 10 results for: pcdna

Showing: 1 - 10 of 10 results

  1. TALEN Plasmids and Kits

    Type
    Collection
    ...rate of Golden Gate assembly. Destination vectors, pcDNA-TAL-NC2 and pCAGGS-TAL-NC2, are mammalian expression...in cultured cells. Golden Gate TALEN 2.0 47388 pcDNA3.1-GoldenGate Charles Gersbach These new destination...activation domain of VP16) at the C terminus. 47389 pcDNA3.1-GoldenGate-VP64 Golden Gate TALEN 2.0 47874 pTALYM3...

  2. Luciferase Plasmid Collection

    Type
    Collection
    ...Nano-lantern/pcDNA3 Nano-lantern CMV Mammalian expression of Nano-lantern Takeharu Nagai 87121 pcDNA-RLuc8 Renilla...upstream of IRES-firefly luciferase. Bryan Welm 87067 pcDNA3.1-ccdB-Nanoluc NanoLuc® Creation of N-terminal Nanoluc...Renilla luciferase is co-expressed. Oskar Laur 87070 pcDNA3.1-Nanoluc-ccdB NanoLuc® Creation of N-terminal Nanoluc...firefly luciferase Eric Campeau 18964 Luciferase-pcDNA3 Firefly CMV Mammalian expression of firefly luciferase...expression of firefly luciferase Javier Alcudia 113442 pcDNA3.1 NL NanoLuc® CMV Mammalian expression of Nanoluc...

  3. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...Cookson 13315 pcDNA-DEST53 PINK1 N-GFP PINK1 GFP CMV Parkinson's Mark Cookson 13316 pcDNA-DEST47 PINK1 ...Aaron Gitler 29609 pcDNA 3.2-FUS-1-526aa-V5 FUS V5 CMV ALS Aaron Gitler 29610 pcDNA 3.2-FUS-1-269aa-V5...Aaron Gitler 29611 pcDNA 3.2-FUS-1-373aa-V5 FUS V5 CMV ALS Aaron Gitler 29612 pcDNA 3.2-FUS-1-413aa-V5...Lindquist 14121 pcDNA3-HtrA2-EGFP HTRA2 GFP CMV Parkinson's L. Miguel Martins 14122 pcDNA3-HtrA2-EGFP S306A...Mombaerts 15938 pcDNA3-HtrA2-FLAG HTRA2 Flag CMV Parkinson's L. Miguel Martins 15939 pcDNA3-HtrA2-FLAG S142D...Cliff Tabin 19457 pcDNA5/FRT/TO HSPA1L HSPA1L CMV Parkinson's Harm Kampinga 19484 pcDNA5/FRT/TO GFP HSPA1L...Kampinga 19511 pcDNA5/FRT/TO V5 HSPA1L HSPA1L V5 CMV Parkinson's Harm Kampinga 19538 pcDNA5/FRT/TO HIS HSPA1L...

  4. Arf GTPase Family

    Type
    Collection
    ...pET), Mammalian (pcDNA3), Gateway GTPase Arf3 377 181 Bacterial (pET), Mammalian (pcDNA3), Gateway GTPase...pET), Mammalian (pcDNA3), Gateway GTPase Arf5 381 180 Bacterial (pET), Mammalian (pcDNA3), Gateway GTPase...pET), Mammalian (pcDNA3), Gateway GTPase Arl3 403 182 Bacterial (pET), Mammalian (pcDNA3), Gateway GTPase...(pET), Mammalian (pcDNA3) GTPase Arfrp1 10139 201 Bacterial (pET), Mammalian (pcDNA3), Gateway GTPase ... (pET), Mammalian (pcDNA3) GAP Arfgap2 84364 521 Bacterial (pET), Mammalian (pcDNA3) GAP Arfgap3 26286...pGEX4T), Mammalian (pcDNA3) GAP ELMOD2 255550 293 Bacterial (pGEX4T), Mammalian (pcDNA3) GAP ELMOD3 84173...GTPase Arf6 382 175 Bacterial (pET), Mammalian (pcDNA3), Gateway GTPase Arl1 400 181 Bacterial (pET), Mammalian...

  5. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ... Backbones Flag Epitope tag c-Flag pcDNA3 or FLAG-HA-pcDNA3.1 - C-terminal Flag or N-terminal FLAG-... including Xenopus HA Epitope tag pcDNA3.1-HA or c-Flag pcDNA3 - Mammalian expression vector with N...C-terminus of BioID2 3xHA-TurboID-NLS_pCDNA3 or 3xHA-miniTurbo-NLS_pCDNA3 - Expresses 3xHA-tagged TurboID...expression plasmid for cloning untagged proteins pcDNA3.1-mCherry - Expresses mCherry tag in mammalian cells...Fluorescent proteins (GFP, mCherry, etc) Localization pcDNA3-EGFP - C-terminal GFP for mammalian expression ...plasmid without effector fusions; 3X Flag tag; 2NLS; pCDNA3 vector backbone, mammalian expression pENTR4-myc-nuc...

  6. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ...Expression ECFP 433 475 13 4.7 Prone to dimerization pcDNA3-CFP - Mammalian Expression pCAG-CFP - Mammalian...Expression EGFP 488 507 34 6 Prone to dimerization pcDNA3-EGFP - Mammalian Expression pCAG-GFP - Mammalian...Clover 505 515 84 6.1 30 min Prone to dimerization pcDNA3-Clover - Mammalian Expression pFA6a-link-yoClover-Kan...Plasmids EYFP 513 527 51 6.9 Prone to dimerization pcDNA3-YFP - Mammalian Expression Topaz 514 527 57 Prone...Expression mCitrine 516 529 59 5.7 Monomer (A206K) pcDNA3 mCitrine LIC cloning vector (6C) - Mammalian Expression...mRuby2 559 600 43 5.3 150 min Prone to dimerization pcDNA3-mRuby2 - Mammalian Expression pFA6a-link-yomRuby2...Bacterial Expression mRFP1 584 607 13 4.5 1 hr Monomer pcDNA3-mRFP - Mammalian Expression pMXs-mRFP1 - Mammalian...

  7. Fluorescent Protein Guide: Subcellular Localization

    Type
    Collection
    ...Extracellular milieu cfSGFP2-N EGFP Ikuo Wada 73208 pcDNA3.1-COXIV-COX8-dL5-2XG4S-mCer3 Mitochondria COX IV-derived...Nuc Nucleus NLS mKalama1 Robert Campbell 73205 pcDNA3.1-NLS-myc-dL5-2xG4S-mCer3 Nucleus NLS (from Mak16p...protein) dL5 FAP; mCerulean3 Marcel Bruchez 73207 pcDNA3.1-KozATG-dL5-2XG4S-mCer3 Cytosol, nucleoplasm None...sequence from p63 mTurquoise2 Dorus Gadella 73206 pcDNA3.1-kappa-myc-dL5-2xG4S-TMst Cell surface (mammalian... Peroxisome SKL mNeonGreen Dorus Gadella 73210 pcDNA3.1-KozATG-myc-dL5-2XG4S-mCer3-SKL Peroxisome SKL ...LC3 mCherry, GFP Anne Brunet 40827 mCherry-hLC3B-pcDNA3.1 Autophagosome LC3 mCherry David Rubinsztein 11546...Endoplasmic Reticulum Sec61 BFP Gia Voeltz 73209 pcDNA3.1-kappa-myc-dL5-2XG4S-mCer3-KDEL Endoplasmic Reticulum...

  8. Cre-lox system

    Type
    Collection
    ...122960 pcDNA3.1_PA-Cre photo-activatable Cre recombinase CMV Mammalian Sato 122961 pcDNA3.1_PA-Cre-Y324F...Binding Domain (EBD) Tdh4 Yeast van Leeuwen 65727 pcDNA3.1-CMV-CFP;UBC-Cre25nt Cre with a 25 nucleotide extracellular...

  9. Genetic Code Expansion

    Type
    Collection
    ...L-lysine (TFA-Lys) Bacterial TAG Thomas Huber 182287 pcDNA3.1(+)_U6 tRNAPyl_CMV NESPylRS(AF) Y306A/Y384F (AF...PylRS M. mazei Bacterial TAA Wenshe Liu 182653 pcDNA3.1(+)_U6 tRNAPyl_CMV NESPylRS(AF)_IRES_eRF1(E55D)...
Showing: 1 - 10 of 10 results