We narrowed to 58,787 results for: Ran

1. 457 p3E-2A-EGFPpA

   Plasmid

   #195967

   Purpose

   Gateway p3E 2A fluorophore

   Depositor

   Kristen Kwan, Christian Mosimann

   Article

   Kemmler et al Development. 2023 Mar 28:dev.201531. doi

   Insert

   2A-EGFP
   

   Use

   Other

   Available Since

   Feb. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1 Flag Runx1 FL (P#1790)

   Plasmid

   #14585

   Depositor

   Matija Peterlin

   Article

   Jiang et al Mol Cell Biol. 2005 Dec . 25(24):10675-8

   Insert

   Runx1 (Runx1 Mouse)
   

   Tags

   Flag

   Expression

   Mammalian

   Available Since

   June 30, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFRTpsiCHECK_SEAP

   Plasmid

   #154461

   Purpose

   Expression of SEAP and firefly luciferase

   Depositor

   Markus Landthaler

   Article

   Zinnall et al Nat Commun. 2022 May 18;13(1):2727. doi:

   Insert

   SEAP
   

   Available Since

   Aug. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET28a MISTIC

   Plasmid

   #85999

   Purpose

   bacterial expression of transmembrane proteins using N-terminal MISTIC and C-terminal HIS6 tag

   Depositor

   Wolfram Antonin

   Article

   Vollmer et al EMBO J. 2012 Oct 17;31(20):4072-84. doi:

   Insert

   N/A
   

   Tags

   HIS6 and MISTIC

   Expression

   Bacterial

   Mutation

   codon optimzed for bacterial expression

   Promoter

   T7

   Available Since

   April 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pUD-MSP1D1

   Plasmid

   #161608

   Purpose

   Bacterial expression of mutated membrane scaffold protein ("ultra-dark" MSP1D1) for Nanodisc formation

   Depositor

   Stephen Sligar

   Article

   McLean et al Anal Biochem. 2020 Oct 15;607:113860. do

   Insert

   UD-MSP1D1
   

   Tags

   7-His tag

   Expression

   Bacterial

   Mutation

   mutant of MSP1D1; all tryptophans and tyrosines w…

   Promoter

   T7

   Available Since

   Nov. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSerOTS-C1* (V70)

   Plasmid

   #188537

   Purpose

   Phosphoserine incorporation into recombinant proteins

   Depositor

   Jesse Rinehart

   Article

   Mohler et al Mol Syst Biol. 2023 Aug 8;19(8):e10591.

   Inserts

   pSerRS
   Ef-pSer
   tRNApSer
   

   Expression

   Bacterial

   Mutation

   mutations resulting from laboratory evolution

   Promoter

   glnS*

   Available Since

   Dec. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. KRAB-dSpCas9

   Plasmid

   #205248

   Purpose

   In vitro transcription plasmid for production of KOX1 KRAB-dSpCas9 mRNA for transcriptional repression (KRAB = Krüppel-associated box). Insert is derived from Addgene plasmid #85449.

   Depositor

   Rasmus Bak

   Article

   Jensen et al Genome Res. 2021 Nov;31(11):2120-2130. d

   Insert

   KRAB-dSpCas9
   

   Use

   CRISPR

   Available Since

   Sept. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. LV-TRE-WT human MyoD-T2A-dsRedExpress2

   Plasmid

   #60628

   Purpose

   Expresses WT human MyoD and dsRedExpress2 in response to doxycycline

   Depositor

   Charles Gersbach

   Article

   Kabadi et al ACS Synth Biol. 2014 Dec 10.

   Insert

   VP64 mouse MyoD (MYOD1 Human)
   

   Use

   Lentiviral

   Promoter

   TRE

   Available Since

   Dec. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. GFP-LacI

   Plasmid

   #183920

   Purpose

   Lac repressor labeled with GFP; can be used to label lacO sites

   Depositor

   Karsten Rippe

   Article

   Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

   Insert

   GFP-LacI (lacI Synthetic)
   

   Expression

   Mammalian

   Mutation

   none

   Promoter

   SV2

   Available Since

   June 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDMS151A_Tet-On-3G

    Plasmid

    #191577

    Purpose

    ClonTech-Takara's doxycycline-inducible, rtTA transactivator (Tet-On-3G) cloned into a 2nd generation lentiviral transfer plasmid (pGIPZ).

    Depositor

    Joshua Leonard

    Article

    Gunnels et al Small. 2022 May;18(19):e2200125. doi: 10

    Insert

    Tet-On(R) 3G
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. Tol2_B-act_dCas9-Vp64-T2A-Citrine

    Plasmid

    #200248

    Purpose

    Zebrafish transgenics; Plasmid encodes dCas9-Vp64 and could be stably integrated in the zebrafish genome using Tol2 transgenesis

    Depositor

    Tatjana Sauka-Spengler

    Article

    Pelea et al bioRxiv 2023.05.08.539738

    Insert

    dCas9-Vp64-T2A-Citrine
    

    Use

    Zebrafish expression

    Available Since

    July 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-EF1a-fDIO-stGtACR2-FusionRed

    Plasmid

    #217975

    Purpose

    AAV transfer plasmid to express stGtACR2-FusionRed in a frt-dependent manner

    Depositor

    Edward Callaway

    Article

    Kirchgessner et al Curr Biol. 2021 Dec 6;31(23):5121-5137.e

    Insert

    stGtACR2-FusionRed
    

    Use

    AAV

    Tags

    FusionRed

    Promoter

    EF1a

    Available Since

    April 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBac{3XP3::EYFP,attP}

    Plasmid

    #86860

    Purpose

    Expresses EYFP in eyes and contains an attP landing site for site-specific attB integration

    Depositor

    David Stern

    Article

    Stern et al G3 (Bethesda). 2017 Apr 3;7(4):1339-1347

    Insert

    attP
    

    Expression

    Insect

    Available Since

    June 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSL4393 (pClpX_Eco)

    Plasmid

    #200907

    Purpose

    Expresses human codon-optimized E. coli ClpX from a CMV promoter.

    Depositor

    Samuel H. Sternberg

    Article

    Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

    Insert

    EcoClpX
    

    Tags

    NLS

    Expression

    Mammalian

    Available Since

    May 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRPF215

    Plasmid

    #106377

    Purpose

    C. difficile transposon mutagenesis system. Shuttle vector containing tet-inducible Himar1 transposase and custom ErmR transposon

    Depositor

    Robert Fagan, Neil Fairweather

    Article

    Dembek et al MBio. 2015 Feb 24;6(2):e02383. doi: 10.1

    Insert

    Ptet-himar1-Ter(slpA)-transposon
    

    Use

    E. coli - c. difficile shuttle vector

    Mutation

    Codon optimised for C. difficile

    Promoter

    Ptet

    Available Since

    March 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSL3713 (pTnsC_Pse)

    Plasmid

    #200900

    Purpose

    Expresses human codon-optimized Pseudoalteromonas TnsC from a CMV promoter.

    Depositor

    Samuel H. Sternberg

    Article

    Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

    Insert

    PseTnsC
    

    Tags

    3xNLS

    Expression

    Mammalian

    Available Since

    May 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTH761-CEN-mCherry_v4

    Plasmid

    #40599

    Depositor

    Tobias von der Haar

    Article

    Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

    Insert

    mCherry (Red Fluorescent Protein variant) with yeast promoter and terminator sequences
    

    Expression

    Yeast

    Mutation

    mCherry ORF partially codon optimised for yeast e…

    Promoter

    GPD (TDH3)

    Available Since

    Oct. 18, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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18. PX459-APP-KO

    Plasmid

    #176487

    Purpose

    To knockout APP gene

    Depositor

    Wade Harper

    Article

    Park et al Nat Commun. 2022 Oct 16;13(1):6112. doi:

    Insert

    gRNA sequence
    

    Use

    CRISPR

    Available Since

    May 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDA077

    Plasmid

    #131388

    Purpose

    L1RP expression

    Depositor

    Kathleen Burns

    Article

    Ardeljan et al Nat Struct Mol Biol. 2020 Feb;27(2):168-

    Insert

    L1RP
    

    Expression

    Mammalian

    Available Since

    June 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV.hSynapsin.(cyto).cpSFGFP.HaloTag

    Plasmid

    #209666

    Purpose

    Non-responsive control

    Depositor

    Tim Brown, HHMI-JRC Tool Translation Team

    Article

    Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

    Insert

    cpSFGFP.HaloTag
    

    Use

    AAV

    Promoter

    hSyn

    Available Since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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