We narrowed to 22,253 results for: his

1. pcDNAM-human eIF2C1-A-Myc

   Plasmid

   #50361

   Purpose

   Expression of human eIF2C1 mutant in mamalian cells

   Depositor

   Kumiko Ui-Tei

   Article

   Doi et al Curr Biol. 2003 Jan 8;13(1):41-6.

   Insert

   eIF2C1 (AGO1 Human)
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   contains PRP motif and PAZ domains

   Promoter

   CMV

   Available Since

   Feb. 4, 2014

   Availability

   Academic Institutions and Nonprofits only

2. pET28b-hTIG3-1-132

   Plasmid

   #100720

   Purpose

   Expression of N-terminal region of human TIG3 in E.coli

   Depositor

   Byung Il Lee

   Article

   Han et al Protein Expr Purif. 2010 May;71(1):103-7

   Insert

   RARRES3 (PLAAT4 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promotor

   Available Since

   Oct. 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pJSC011 - Bacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9), HNH FRET variant

   Plasmid

   #101229

   Purpose

   Bacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9), HNH FRET variant

   Depositor

   Jennifer Doudna, Keith Joung

   Article

   Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

   Insert

   SpCas9 variant C80S/C574S/S355C/S867C/N692A/M694A/Q695A/H698A
   

   Use

   Tags

   10x His, MBP, and TEV site

   Expression

   Bacterial

   Mutation

   C80S, C574S, S355C, S867C, N692A, M694A, Q695A an…

   Promoter

   T7

   Available Since

   Oct. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pet23a_RBPMS_20_101

   Plasmid

   #65733

   Purpose

   plasmid for bacterial overexpression of RBPMS

   Depositor

   Thomas Tuschl

   Article

   Farazi et al RNA. 2014 Jul;20(7):1090-102. doi: 10.12

   Insert

   RBPMS (RBPMS Human)
   

   Use

   Tags

   His Tag

   Expression

   Bacterial

   Mutation

   deletion construct encoding amino acids 20-101

   Promoter

   T7

   Available Since

   Aug. 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pET-22HT-MSI1(7-192)R53E/R59E/R61E

   Plasmid

   #60355

   Purpose

   R53E R59E R61E triple mutant of the mouse MSI1 RBD

   Depositor

   Sean Ryder

   Article

   Clingman et al Elife. 2014 Jun 16:e02848. doi: 10.7554/

   Insert

   mouse MSI1 (7-192) (Msi1 Mouse)
   

   Use

   Tags

   His and TEV protease site

   Expression

   Bacterial

   Mutation

   R53E, R59E and R61E mutations; contains amino aci…

   Promoter

   T7

   Available Since

   Oct. 27, 2014

   Availability

   Academic Institutions and Nonprofits only

6. EGFP-hPMCA2x/b delta6

   Plasmid

   #47587

   Depositor

   Emanuel Strehler

   Article

   Chicka et al J Biol Chem. 2003 May 16;278(20):18464-7

   Insert

   PMCA2x/b delta6 (ATP2B2 Human)
   

   Use

   Tags

   EGFP-HA-His

   Expression

   Mammalian

   Mutation

   6 C-terminal AA deleted (needed for PDZ binding)

   Promoter

   CMV

   Available Since

   Oct. 4, 2013

   Availability

   Academic Institutions and Nonprofits only

7. pMCSG49-AlleyCat7

   Plasmid

   #42591

   Depositor

   Ivan Korendovych

   Article

   Moroz et al Angew Chem Int Ed Engl. 2013 Apr 29. doi

   Insert

   mutated C-terminal portion of calmodulin gene (M76-K148)
   

   Use

   Tags

   His and TEV

   Expression

   Bacterial

   Mutation

   changed Phenylalanine 92 to Glutamine acid, Isole…

   Promoter

   T7

   Available Since

   March 21, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. D10 pAcGP67a

   Plasmid

   #41812

   Depositor

   Chris Garcia

   Article

   Levin et al Nature. 2012 Mar 25;484(7395):529-33. do

   Insert

   D10 Super-2 (IL2 Human)
   

   Use

   Tags

   8x His

   Expression

   Insect

   Mutation

   Promoter

   Polyhedron

   Available Since

   Jan. 8, 2013

   Availability

   Academic Institutions and Nonprofits only

9. pJ145

   Plasmid

   #29491

   Depositor

   Jonathan Tait

   Article

   Jin et al J Biol Chem 2004; 279:40351-57.

   Insert

   annexin V-128 (ANXA5 Human)
   

   Use

   Tags

   (Met)-Ala-Gly-Gly-Cys-Gly-His

   Expression

   Bacterial

   Mutation

   Deletion of the initiator Met at position 1 of wi…

   Promoter

   Available Since

   June 7, 2011

   Availability

   Academic Institutions and Nonprofits only

10. pQTEV-C21orf7

    Plasmid

    #34810

    Depositor

    Konrad Buessow

    Article

    Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

    Insert

    C21orf7 (C21orf7 Human)
    

    Use

    Tags

    His and TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 22, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. Tip alpha K102A/K104A (21-192)

    Plasmid

    #23159

    Depositor

    SeWon Suh

    Article

    Jang et al J Mol Biol. 2009 Sep 11. 392(1):191-7.

    Insert

    TNF-α-inducing protein
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    changed lysine 102 and lysine 104 to alanine, and…

    Promoter

    Available Since

    April 16, 2010

    Availability

    Academic Institutions and Nonprofits only

12. CtkA_full_pET21a

    Plasmid

    #27428

    Depositor

    SeWon Suh

    Article

    Kim et al Proc Natl Acad Sci U S A. 2010 Dec 14;10

    Insert

    Cell translocating kinas A (jhp0940 H. pylori)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    May 2, 2011

    Availability

    Academic Institutions and Nonprofits only

13. CtkA_300_pET21a

    Plasmid

    #27429

    Depositor

    SeWon Suh

    Article

    Kim et al Proc Natl Acad Sci U S A. 2010 Dec 14;10

    Insert

    Cell translocating kinas A (jhp0940 H. pylori)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    residues 301-325 are truncated

    Promoter

    Available Since

    May 2, 2011

    Availability

    Academic Institutions and Nonprofits only

14. CtkA_D155Q_pET21a

    Plasmid

    #27430

    Depositor

    SeWon Suh

    Article

    Kim et al Proc Natl Acad Sci U S A. 2010 Dec 14;10

    Insert

    Cell translocating kinas A (jhp0940 H. pylori)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    D155Q

    Promoter

    Available Since

    May 2, 2011

    Availability

    Academic Institutions and Nonprofits only

15. CtkA_D179Q_pET21a

    Plasmid

    #27431

    Depositor

    SeWon Suh

    Article

    Kim et al Proc Natl Acad Sci U S A. 2010 Dec 14;10

    Insert

    Cell translocating kinas A (jhp0940 H. pylori)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    D179Q

    Promoter

    Available Since

    May 2, 2011

    Availability

    Academic Institutions and Nonprofits only

16. CtkA_D155Q/D179Q_pET21a

    Plasmid

    #27432

    Depositor

    SeWon Suh

    Article

    Kim et al Proc Natl Acad Sci U S A. 2010 Dec 14;10

    Insert

    Cell translocating kinas A (jhp0940 H. pylori)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    D155Q/D179Q

    Promoter

    Available Since

    May 2, 2011

    Availability

    Academic Institutions and Nonprofits only

17. GST-MED9

    Plasmid

    #15408

    Depositor

    Ronald Conaway, Joan Conaway

    Article

    Tomomori-Sato et al J Biol Chem. 2004 Feb 13. 279(7):5846-51

    Insert

    MED9 (Med9 Mouse)
    

    Use

    Tags

    GST and His

    Expression

    Bacterial

    Mutation

    N/A

    Promoter

    Available Since

    Aug. 10, 2007

    Availability

    Academic Institutions and Nonprofits only

18. pET41a-MED31

    Plasmid

    #15437

    Depositor

    Ronald Conaway, Joan Conaway

    Article

    Sato et al J Biol Chem. 2003 Apr 25. 278(17):15123-

    Insert

    MED31 (Med31 Mouse)
    

    Use

    Tags

    GST and His

    Expression

    Bacterial

    Mutation

    N/A

    Promoter

    Available Since

    Aug. 10, 2007

    Availability

    Academic Institutions and Nonprofits only

19. GFP-Nsp3(1-111)

    Plasmid

    #242947

    Purpose

    GFP and the Ubl1 domain of the SARS-CoV-2 Nsp3 protein (aa 1-111)

    Depositor

    Benjamin Schuster

    Article

    Favetta et al bioRxiv [Preprint]. 2025 Jun 10:2024.10.

    Insert

    GFP-Nsp3(1-111) (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Sept. 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. FUS LC QtoA

    Plasmid

    #226609

    Purpose

    All glutamines to alanine

    Depositor

    Nicolas Fawzi

    Article

    Wake et al Nat Chem Biol. 2025 Jul;21(7):1076-1088.

    Insert

    6*His-TEV-FUS LC QtoA (FUS Human)
    

    Use

    Tags

    6His

    Expression

    Bacterial

    Mutation

    All glutamine to alanine

    Promoter

    T7

    Available Since

    July 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pRSET A-pcStar

    Plasmid

    #226339

    Purpose

    Bacterial expression of RSFP pcStar; photoconvertible red fluorescent protein with high brightness, high photoconversion contrast, and early detectable fluorescence

    Depositor

    Pingyong Xu

    Article

    Zhang et al Nano Lett. 2020 Apr 8;20(4):2197-2208. d

    Insert

    pcStar
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    June 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. Ce iPGM-C-HiBiT

    Plasmid

    #207120

    Purpose

    Bacterially expressed iPGM-HiBiT for Ni-NTA purification

    Depositor

    James Inglese

    Article

    Ciulla et al Nat Commun. 2025 May 10;16(1):4342. doi:

    Insert

    C. elegans-iPGM HiBIT His10 (ipgm-1 Nematode)
    

    Use

    Tags

    HiBiT Tag and Thrombin cleavage site + 10x His Ta…

    Expression

    Bacterial

    Mutation

    Codon optmization for expression in BL21DE3

    Promoter

    T7 Promotor

    Available Since

    May 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. Bm iPGM-HiBiT

    Plasmid

    #218483

    Purpose

    Bacterially expressed iPGM-HiBiT for Ni-NTA purification

    Depositor

    James Inglese

    Article

    Ciulla et al Nat Commun. 2025 May 10;16(1):4342. doi:

    Insert

    Bm-iPGM-C-HiBIT-Thrombin-6XHis
    

    Use

    Tags

    HiBiT Tag and His-tag with thrombin site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    May 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. NP-11-63

    Plasmid

    #234358

    Purpose

    Expressing and purifying R.rubrum rubisco using a his-sumo tag

    Depositor

    David Savage

    Article

    Prywes et al Nature. 2025 Jan 22. doi: 10.1038/s41586

    Insert

    R.rubrum rubisco
    

    Use

    Synthetic Biology

    Tags

    N-term SUMO tag

    Expression

    Mutation

    Promoter

    T7

    Available Since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pYI2294

    Plasmid

    #228352

    Purpose

    Methanol-inducible ALX-0081 expression

    Depositor

    Jun Ishii

    Article

    Tominaga et al Nat Commun. 2024 Dec 19;15(1):10653. doi

    Insert

    MFalpha(2xAdv)-LE-ALX-0081-3A-FLAG-His
    

    Use

    Affinity Reagent/ Antibody

    Tags

    AAA linker followed by FLAG-His6 and MF_ secretio…

    Expression

    Yeast

    Mutation

    Promoter

    KpAOX1 promoter

    Available Since

    March 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pYI2216

    Plasmid

    #228349

    Purpose

    2,4-Diacetylphloroglucinol (DAPG)-regulatable ALX-0081 expression

    Depositor

    Jun Ishii

    Article

    Tominaga et al Nat Commun. 2024 Dec 19;15(1):10653. doi

    Insert

    MFalpha(2xAdv)-LE-ALX-0081-3A-FLAG-His
    

    Use

    Affinity Reagent/ Antibody

    Tags

    AAA linker followed by FLAG-His6 and MF_ secretio…

    Expression

    Yeast

    Mutation

    Promoter

    2,4-Diacetylphloroglucinol-regulatable synthetic …

    Available Since

    March 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pYI2292

    Plasmid

    #228350

    Purpose

    Methanol-inducible ALX-0171 expression

    Depositor

    Jun Ishii

    Article

    Tominaga et al Nat Commun. 2024 Dec 19;15(1):10653. doi

    Insert

    MFalpha(2xAdv)-LE-ALX-0171-3A-FLAG-His
    

    Use

    Affinity Reagent/ Antibody

    Tags

    AAA linker followed by FLAG-His6 and MF_ secretio…

    Expression

    Yeast

    Mutation

    Promoter

    KpAOX1 promoter

    Available Since

    March 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pYI2293

    Plasmid

    #228351

    Purpose

    Constitutive ALX-0081 expression

    Depositor

    Jun Ishii

    Article

    Tominaga et al Nat Commun. 2024 Dec 19;15(1):10653. doi

    Insert

    MFalpha(2xAdv)-LE-ALX-0081-3A-FLAG-His
    

    Use

    Affinity Reagent/ Antibody

    Tags

    AAA linker followed by FLAG-His6 and MF_ secretio…

    Expression

    Yeast

    Mutation

    Promoter

    KpGAPDH promoter

    Available Since

    March 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pMT1461

    Plasmid

    #228319

    Purpose

    Doxycycline-regulatable ALX-0081 expression

    Depositor

    Jun Ishii

    Article

    Tominaga et al Nat Commun. 2024 Dec 19;15(1):10653. doi

    Insert

    MFalpha(2xAdv)-ALX-0081-3A-FLAG-His
    

    Use

    Affinity Reagent/ Antibody

    Tags

    AAA linker followed by FLAG-His6 and MF_ secretio…

    Expression

    Yeast

    Mutation

    Promoter

    Doxycycline-regulatable synthetic promoter (KpTc7…

    Available Since

    March 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCA528 huPQBP1

    Plasmid

    #217449

    Purpose

    Expresses full-length human PQBP1 from a His-SUMO bacterial expression vector

    Depositor

    Jarrod Johnson

    Article

    Scott et al PLoS Pathog. 2025 Jan 28;21(1):e1012206.

    Insert

    PQBP1 (PQBP1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Jan. 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pCA528 IST1, residues 316-366, C-Cys

    Plasmid

    #191234

    Purpose

    Bacterial expression for IST1 residues 316-366, contains non-native C-terminal Cysteine for labeling

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    IST1 (IST1 Human)
    

    Use

    Tags

    HIS-SUMO

    Expression

    Bacterial

    Mutation

    Residues 316-366, contains nonnative C-terminus f…

    Promoter

    T7

    Available Since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pCS2-MfnG-EcYRS-EGFP*

    Plasmid

    #191119

    Purpose

    A plasmid for incorporation of O-Me-Tyr into an EGFP reporter without O-Me-Tyr feeding for zebrafish

    Depositor

    Han Xiao

    Article

    Wu et al Protein Sci. 2022 Oct;31(10):e4443. doi:

    Insert

    MfnG - P2A - Mutant E. coli TyrRS - T2A - enhanced green fluorescence protein
    

    Use

    Tags

    His tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pCA528 CHMP1A 140-196

    Plasmid

    #104593

    Purpose

    Expresses C-terminal 140-196 residues of CHMP1A with non-native N-term cysteine for fluorescent labeling; Internal ID: WISP18-1

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    CHMP1A residues 140-196 (CHMP1A Human)
    

    Use

    Tags

    His-SUMO

    Expression

    Bacterial

    Mutation

    Contains nonnative Gly-Cys for fluorescent labeli…

    Promoter

    T7

    Available Since

    Nov. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pCA528 CHMP2A 152-222

    Plasmid

    #104594

    Purpose

    Expresses C-terminal 152-222 residues of CHMP2A with non-native N-term cysteine for fluorescent labeling; Internal ID: WISP18-3

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    CHMP2A residues 152-222 (CHMP2A Human)
    

    Use

    Tags

    His-SUMO

    Expression

    Bacterial

    Mutation

    Contains nonnative Gly-Cys for fluorescent labeli…

    Promoter

    T7

    Available Since

    Nov. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pCA528 CHMP2B 141-213

    Plasmid

    #104595

    Purpose

    Expresses C-terminal 141-213 residues of CHMP2B with non-native N-term cysteine for fluorescent labeling; Internal ID: WISP18-4

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    CHMP2B residues 141-213 (CHMP2B Human)
    

    Use

    Tags

    His-SUMO

    Expression

    Bacterial

    Mutation

    Contains nonnative Gly-Cys for fluorescent labeli…

    Promoter

    T7

    Available Since

    Nov. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pCA528 CHMP6 145-201

    Plasmid

    #104597

    Purpose

    Expresses C-terminal 145-201 residues of CHMP6 with non-native N-term cysteine for fluorescent labeling; Internal ID: WISP18-11

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    CHMP6 residues 145-201 (CHMP6 Human)
    

    Use

    Tags

    His-SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Nov. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pCA528 CHMP3 159-222

    Plasmid

    #104599

    Purpose

    Expresses C-terminal 159-222 residues of CHMP3 with non-native N-term cysteine for fluorescent labeling; Internal ID: WISP18-5

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    CHMP3 residues 159-222 (CHMP3 Human)
    

    Use

    Tags

    His-SUMO

    Expression

    Bacterial

    Mutation

    Contains nonnative Gly-Cys for fluorescent labeli…

    Promoter

    T7

    Available Since

    Nov. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pCA528 CHMP4A 153-222

    Plasmid

    #108256

    Purpose

    Expresses C-terminal 153-222 residues of CHMP4A with non-native N-term cysteine for fluorescent labeling; Internal ID: WISP18-6

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    CHMP4A residues 153-222 (CHMP4A Human)
    

    Use

    Tags

    His-SUMO

    Expression

    Bacterial

    Mutation

    Contains nonnative Gly-Cys for fluorescent labeli…

    Promoter

    T7

    Available Since

    Nov. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pCA528 CHMP4C 156-233

    Plasmid

    #108257

    Purpose

    Expresses C-terminal 156-233 residues of CHMP4C with non-native N-term cysteine for fluorescent labeling; Internal ID: WISP18-9

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    CHMP4C residues 156-233 (CHMP4C Human)
    

    Use

    Tags

    His-SUMO

    Expression

    Bacterial

    Mutation

    Contains nonnative Gly-Cys for fluorescent labeli…

    Promoter

    T7

    Available Since

    Nov. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pCA528 CHMP5 159-222

    Plasmid

    #108259

    Purpose

    Expresses C-terminal 159-222 residues of CHMP5 with non-native N-term cysteine for fluorescent labeling; Internal ID: WISP18-10

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    CHMP5 residues 148-219 (CHMP5 Human)
    

    Use

    Tags

    His-SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Nov. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge