We narrowed to 6,088 results for: pcDNA 3.1

1. PA-RL-BAD

   Plasmid

   #78859

   Purpose

   DULIP positive control (bait) for assay establishment

   Depositor

   Erich Wanker

   Article

   Trepte et al J Mol Biol. 2015 Oct 23;427(21):3375-88.

   Insert

   BCL2 associated agonist of cell death (BAD Human)
   

   Use

   Expression vector

   Tags

   Protein A, Renilla luciferase

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJO427

   Plasmid

   #111139

   Purpose

   URT-type vector for Glu-mIκBα expression in Mammalian cells

   Depositor

   Alexander Varshavsky

   Article

   Piatkov et al Proc Natl Acad Sci U S A. 2014 Mar 4;111

   Insert

   flag-lacZ25-119-DHFR-UbK48R-Glu51-mIκBα (Ikbkb Mouse)
   

   Tags

   Flag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. CFP-GIV-CT FA

   Plasmid

   #69533

   Purpose

   Mammalian expression of The CFP-tagged GIV-CT-FA biosensors

   Depositor

   Pradipta Ghosh

   Article

   Midde et al Proc Natl Acad Sci U S A. 2015 Mar 3;112

   Insert

   GIV (CCDC88A Human)
   

   Tags

   CFP

   Expression

   Mammalian

   Mutation

   C terminal amino acids S1660-S1870 of human GIV w…

   Promoter

   CMV

   Available Since

   Jan. 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. CFP-GIV-CT WT

   Plasmid

   #69532

   Purpose

   Mammalian expression of The CFP-tagged GIV-CT biosensors

   Depositor

   Pradipta Ghosh

   Article

   Midde et al Proc Natl Acad Sci U S A. 2015 Mar 3;112

   Insert

   GIV (CCDC88A Human)
   

   Tags

   CFP

   Expression

   Mammalian

   Mutation

   C terminal amino acids S1660-S1870 of human GIV

   Promoter

   CMV

   Available Since

   Oct. 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. FLAG-MED31

   Plasmid

   #15427

   Depositor

   Joan Conaway, Ronald Conaway

   Article

   Sato et al J Biol Chem. 2003 Apr 25. 278(17):15123-

   Insert

   MED31 (Med31 Mouse)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   N/A

   Available Since

   Aug. 15, 2007

   Availability

   Academic Institutions and Nonprofits only

6. pTE4565

   Plasmid

   #88903

   Purpose

   Expresses inactive/dead, humanized MbCpf1 nuclease

   Depositor

   Ervin Welker

   Article

   Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

   Insert

   hMbCpf1(D986A)
   

   Use

   CRISPR

   Tags

   3xHA and NLS

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. Opto-GPR182

   Plasmid

   #106061

   Purpose

   Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR182; Opto-GPR182; F3)

   Depositor

   Harald Janovjak

   Article

   Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

   Insert

   Opto-GPR182 (GPR182 Human, Bovine)
   

   Tags

   Rhodopsin-1D4 and VSV-G

   Expression

   Mammalian

   Mutation

   Chimeric receptor protein

   Promoter

   CMV

   Available Since

   Jan. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. Opto-GPR17

   Plasmid

   #106005

   Purpose

   Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR17; Opto-GPR17; A7)

   Depositor

   Harald Janovjak

   Article

   Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

   Insert

   Opto-GPR17 (GPR17 Human, Bovine)
   

   Tags

   Rhodopsin-1D4 and VSV-G

   Expression

   Mammalian

   Mutation

   Chimeric receptor protein

   Promoter

   CMV

   Available Since

   Jan. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pJO428

   Plasmid

   #111141

   Purpose

   URT-type vector for Val-mIκBα expression in Mammalian cells

   Depositor

   Alexander Varshavsky

   Article

   Piatkov et al Proc Natl Acad Sci U S A. 2014 Mar 4;111

   Insert

   flag-lacZ25-119-DHFR-UbK48R-Val51-mIκBα (Ikbkb Mouse)
   

   Tags

   Flag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Opto-GPR37L1

    Plasmid

    #106020

    Purpose

    Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR37L1; Opto-GPR37L1; B10)

    Depositor

    Harald Janovjak

    Article

    Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

    Insert

    Opto-GPR37L1 (GPR37L1 Human, Bovine)
    

    Tags

    Rhodopsin-1D4 and VSV-G

    Expression

    Mammalian

    Mutation

    Chimeric receptor protein

    Promoter

    CMV

    Available Since

    Jan. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJHG004

    Plasmid

    #126455

    Purpose

    Expresses dTdT in mammalian cells.

    Depositor

    Chang Liu

    Article

    Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

    Insert

    dTdT (DNTT Human)
    

    Expression

    Mammalian

    Mutation

    Added D343E and D345E to inactivate the enzyme.

    Promoter

    CMV

    Available Since

    June 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTE4567

    Plasmid

    #107557

    Purpose

    Expresses human codon optimized inactive MbCpf1(dead2) in mammalian cells.

    Depositor

    Ervin Welker

    Article

    Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

    Insert

    hMbCpf1(dead2)
    

    Tags

    3xHA and NLS

    Expression

    Mammalian

    Mutation

    E1080A

    Promoter

    CMV

    Available Since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. Opto-GPR19

    Plasmid

    #106007

    Purpose

    Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR19; Opto-GPR19; A9)

    Depositor

    Harald Janovjak

    Article

    Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

    Insert

    Opto-GPR19 (GPR19 Human, Bovine)
    

    Tags

    Rhodopsin-1D4 and VSV-G

    Expression

    Mammalian

    Mutation

    Chimeric receptor protein

    Promoter

    CMV

    Available Since

    Jan. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTBL681 CHYRON4 integration construct

    Plasmid

    #126449

    Purpose

    To integrate the CHYRON4 locus at AAVS1 in human cells.

    Depositor

    Chang Liu

    Article

    Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

    Inserts

    pU6/3xLacO-CHYRON4 hgRNA
    pCMV-puro
    

    Expression

    Mammalian

    Mutation

    The SpCas9 sgRNA constant region is mutated to ma…

    Promoter

    CMV and human U6/3xLacO

    Available Since

    June 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. HA-human-BNIP3 delta Exon2

    Plasmid

    #100782

    Purpose

    Mammalian expression of BNIP3 delta Exon2 splice variant

    Depositor

    Joseph Gordon

    Article

    Field et al Cell Death Discov. 2018 Sep 21;4:37. doi

    Insert

    human-BNIP3 delta Exon2 (BNIP3 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Exon2 deletion

    Promoter

    CMV

    Available Since

    Sept. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. AIDx-ID-1058

    Plasmid

    #135347

    Purpose

    Expression of intradomain insertion of truncated human cytosine deaminase, hAIDx, at position 1058 of nSpyCas9 with uracil DNA glycosylase inhibitor (UGI) at the C terminus

    Depositor

    Alex Hewitt

    Article

    Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

    Insert

    nSpyCas9-hAIDx-ID-1058-UGI
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    WT

    Available Since

    Oct. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. Opto-GPR75

    Plasmid

    #106032

    Purpose

    Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR75; Opto-GPR75; C10)

    Depositor

    Harald Janovjak

    Article

    Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

    Insert

    Opto-GPR75 (GPR75 Human, Bovine)
    

    Tags

    Rhodopsin-1D4 and VSV-G

    Expression

    Mammalian

    Mutation

    Chimeric receptor protein

    Promoter

    CMV

    Available Since

    Jan. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCMV-ABEMAX-NLS-P2A_BLAST

    Plasmid

    #135355

    Purpose

    Expression of ABEMax (wildtype Tada monomer coupled with evolved Tada monomer) with dual C-terminal NLS in a single operon encoding P2A blasticidine resistance

    Depositor

    Alex Hewitt

    Article

    Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

    Insert

    ABEMax-2XNLS
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    WT

    Available Since

    Oct. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. FRT2-H2B-EGFP-V5-2A (IG157)

    Plasmid

    #99623

    Purpose

    To clone gene of interest downstream of FRT2-H2B-EGFP-V5-2A cassette

    Depositor

    Rui Benedito

    Article

    Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

    Insert

    H2B-EGFP-V5
    

    Tags

    T2A

    Expression

    Mammalian

    Available Since

    Sept. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Opto-GPR61

    Plasmid

    #106027

    Purpose

    Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR61; Opto-GPR61; C5)

    Depositor

    Harald Janovjak

    Article

    Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

    Insert

    Opto-GPR61 (GPR61 Human, Bovine)
    

    Tags

    Rhodopsin-1D4 and VSV-G

    Expression

    Mammalian

    Mutation

    Chimeric receptor protein

    Promoter

    CMV

    Available Since

    Jan. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge