We narrowed to 5,913 results for: pcDNA 3.1

1. pGAL4-DBD-HOXA13-58A-IDR

   Plasmid

   #145238

   Purpose

   Expresses fusion of GAL4 DNA-binding domain and HOXA13-58A IDR

   Depositor

   Denes Hnisz

   Article

   Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

   Insert

   HOXA13-58A-IDR (HOXA13 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   A-repeats deleted

   Promoter

   Available since

   Sept. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. mVenus-proα1(I)

   Plasmid

   #119845

   Purpose

   Expresses mouse Type I procollagen α1 chain (Col1a1) with mVenus replacing exon 2-3 retaining N-propeptide minor triple helix and N-terminal cleavage site

   Depositor

   Sergey Leikin

   Article

   Omari et al Proc Natl Acad Sci U S A. 2018 Oct 23;11

   Insert

   Type I procollagen α1 chain (Col1a1 Mouse)
   

   Use

   Tags

   mVenus

   Expression

   Mammalian

   Mutation

   Col1a1 exons 2-3 replaced by fluorescent tag

   Promoter

   CMV

   Available since

   Jan. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. FLAG-FBXL17

   Plasmid

   #236188

   Purpose

   Expression of N-terminal tagged FLAG-FBXL17 (H. sapiens, codon-optimized) in human cell lines.

   Depositor

   Michele Pagano

   Article

   Cao et al Cell. 2024 Oct 30:S0092-8674(24)01188-7.

   Insert

   FLAG-FBXL17 (FBXL17 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pStreptagIIx2 Alfatag 3C InsR EABR

   Plasmid

   #234996

   Purpose

   For production of Extracellular Vesicles (EVs), with the Insulin Receptor Strep-tag II labeled on their surface

   Depositor

   Daniel Leahy, Monica Gonzalez-Magaldi

   Article

   Gonzalez-Magaldi et al bioRxiv [Preprint]. 2024 Nov 28:2024.11.

   Insert

   InsR-EABR (INSR Human)
   

   Use

   Tags

   2xStreptag-II, Alfatag, HRV 3C site

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. GFP-cCGNL1-Myc

   Plasmid

   #236516

   Purpose

   For expression of Paracingulin (dog) in mammalian cells

   Depositor

   Sandra Citi

   Article

   Rouaud et al J Cell Biol. 2023 Jul 3;222(7):e20220806

   Insert

   CGNL1 (CGNL1 Canis lupus familiaris (dog))
   

   Use

   Tags

   GFP and Myc

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Available since

   April 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. MPC1-AA-FLAG

   Plasmid

   #228904

   Purpose

   Expression of mouse MPC1 in which K45 and K46 motif sites were replaced with alanine blocking acetylation with a C terminal FLAG tag

   Depositor

   Ghazaleh Ashrafi

   Article

   Tiwari et al Sci Adv. 2024 Nov 15;10(46):eadp7423. do

   Insert

   MPC1 (Mpc1 Mouse)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   K45 and K46 motif sites were both replaced with a…

   Promoter

   CMV

   Available since

   Feb. 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. CIRC#del

   Plasmid

   #186344

   Purpose

   Contains VEGFA exon 7 and short intronic flanking regions not sufficient for expression of hsa_circ_0076611 (circVEGF)

   Depositor

   Giovanni Blandino

   Article

   Turco et al Commun Biol. 2022 Jun 16;5(1):598. doi:

   Insert

   VEGFA (VEGFA Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. Flag-SKP1-K142R

   Plasmid

   #213423

   Purpose

   Expresses human SKP1 K142R mutant in mammalian cells

   Depositor

   Michele Pagano

   Article

   Li et al Sci Adv. 2023 Oct 13;9(41):eadh1134. doi

   Insert

   human Flag tagged SKP1-K142R (SKP1 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. Flag-SKP1-97-101-5A

   Plasmid

   #213642

   Purpose

   Expresses human SKP1-97-101-5A mutant in mammalian cells

   Depositor

   Michele Pagano

   Article

   Li et al Sci Adv. 2023 Oct 13;9(41):eadh1134. doi

   Insert

   human Flag tagged SKP1--97-101-5A (SKP1 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Flag-SKP1-K163R

    Plasmid

    #213424

    Purpose

    Expresses human SKP1 K163R mutant in mammalian cells

    Depositor

    Michele Pagano

    Article

    Li et al Sci Adv. 2023 Oct 13;9(41):eadh1134. doi

    Insert

    human Flag tagged SKP1-K163R (SKP1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. anti-GFAP R416WT scFv [N206B/9] with sortase tag

    Plasmid

    #204420

    Purpose

    Mammalian Expression of anti-GFAP scFv with a sortase tag for direct dye conjugation. Derived from hybridoma N206B/9.

    Depositor

    Jeff Lichtman

    Article

    Han et al bioRxiv. 2023 Jun 8:2023.05.20.540091. d

    Insert

    Recombinant mouse scFv targeting GFAP R416WT (Homo sapiens)
    

    Use

    Tags

    6xHis tag and Sortase tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pSI-218

    Plasmid

    #197139

    Purpose

    All-in-vector of SpCas9 gRNA cloning sites (BsmBI) and CMVp-Target-AID expression unit

    Depositor

    Nozomu Yachie

    Article

    Ishiguro et al Nat Biotechnol. 2025 May 21. doi: 10.103

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    SpCas9-PmCDA1-UGI

    Expression

    Mammalian

    Mutation

    SpCas9(D10A)

    Promoter

    Available since

    May 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. CIRC

    Plasmid

    #186343

    Purpose

    Contains VEGFA exon 7 and part of intronic flanking regions enabling expression of hsa_circ_0076611 (circVEGF)

    Depositor

    Giovanni Blandino

    Article

    Turco et al Commun Biol. 2022 Jun 16;5(1):598. doi:

    Insert

    VEGFA (VEGFA Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. S1 1029 CP APOBEC3 no UGI

    Plasmid

    #135352

    Purpose

    Expression of circular permutant of nSpyCas9 lacking uracil DNA glycosylase inhibitor at amino acid position 1029 encoding rat cytosine deaminase, rAPOBEC3 at the N-terminus

    Depositor

    Alex Hewitt

    Article

    Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

    Insert

    rAPOBEC3-nSpyCas9 aa 1029
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    Oct. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. S1 1029 CP ABEMax strategy 2

    Plasmid

    #135358

    Purpose

    Expression of circular permutant of nSpyCas9 at amino acid position 1029 encoding de-coupled ABEMax (wildtype Tada monomer at N terminus, evolved Tada monomer at C terminus)

    Depositor

    Alex Hewitt

    Article

    Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

    Insert

    WT Tada-nSpyCas9 aa 1029-evolved Tada
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    Oct. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pGAL4-DBD-HOXD13-Adel-15A-IDR

    Plasmid

    #145243

    Purpose

    Expresses fusion of GAL4 DNA-binding domain and HOXD13-Adel-15A IDR

    Depositor

    Denes Hnisz

    Article

    Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

    Insert

    HOXD13-Adel-15A-IDR (HOXA13 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    A-repeats deleted

    Promoter

    Available since

    Sept. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pGAL4-DBD-HOXA13-44A-IDR

    Plasmid

    #145237

    Purpose

    Expresses fusion of GAL4 DNA-binding domain and HOXA13-44A IDR

    Depositor

    Denes Hnisz

    Article

    Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

    Insert

    HOXA13-44A-IDR (HOXA13 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    A-repeats deleted

    Promoter

    Available since

    Sept. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pGAL4-DBD-HOXA13-15A-IDR

    Plasmid

    #145236

    Purpose

    Expresses fusion of GAL4 DNA-binding domain and HOXA13-15A IDR

    Depositor

    Denes Hnisz

    Article

    Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

    Insert

    HOXA13-15A-IDR (HOXA13 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    A-repeats deleted

    Promoter

    Available since

    Sept. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pGAL4-DBD-HEY2-Adel-IDR

    Plasmid

    #145234

    Purpose

    Expresses fusion of GAL4 DNA-binding domain and HEY2-Adel IDR

    Depositor

    Denes Hnisz

    Article

    Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

    Insert

    HEY2-Adel-IDR (HEY2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    A-repeats deleted

    Promoter

    Available since

    Sept. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGAL4-DBD-BHLHE41-Adel-IDR

    Plasmid

    #145228

    Purpose

    Expresses fusion of GAL4 DNA-binding domain and BHLHE41-Adel IDR

    Depositor

    Denes Hnisz

    Article

    Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

    Insert

    BHLHE41-Adel-IDR (BHLHE41 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    A-repeats deleted

    Promoter

    Available since

    Sept. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge