We narrowed to 6,515 results for: sas;

1. pUPD2 Register 1/3 Tnos:YFP:5'UTR (GB1507)

   Plasmid

   #160583

   Purpose

   Part 1/3 for assembly of site-specific recombination based Registers. Composed of the reversed sequences of the TNos terminator,YFP CDS and 5' UTR of 35S promoter (TNos:YFP:5'UTR)

   Depositor

   Diego Orzaez

   Article

   Bernabe-Orts et al Nucleic Acids Res. 2020 Apr 6;48(6):3379

   Insert

   YFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   Available Since

   Aug. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET15b p97-Strep-His E314Amb

   Plasmid

   #169021

   Purpose

   Expresses p97-Strep-His E314Amb (incorporates noncanonical amino acid at position 314 via amber suppression) in bacteria

   Depositor

   Hemmo Meyer

   Article

   Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

   Insert

   p97
   

   Use

   Tags

   His and SBP

   Expression

   Bacterial

   Mutation

   E314TAG

   Promoter

   T7

   Available Since

   July 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEGB_3alpha2 OplacI:mini35S:RDF:Tnos (GB1534)

   Plasmid

   #160617

   Purpose

   TU for the regulated expression of the PhiC31 phage recombination directionality factor (RDF) gene driven by a synthetic promoter consisting of the LacI operator fused to the minimal 35S promoter.

   Depositor

   Diego Orzaez

   Article

   Bernabe-Orts et al Nucleic Acids Res. 2020 Apr 6;48(6):3379

   Insert

   RDF
   

   Use

   Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   mini35s

   Available Since

   March 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pUPD2 PhiC31 RL (attR:P35S:TMtb:attL) (GB1506)

   Plasmid

   #160582

   Purpose

   35S promoter inverted sequence and the Mtb terminator flanked by two opposing PhiC31 site-specific recombination sites (attR and attL)

   Depositor

   Diego Orzaez

   Article

   Bernabe-Orts et al Nucleic Acids Res. 2020 Apr 6;48(6):3379

   Insert

   attR:P35S:TMtb:attL
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   Available Since

   March 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pUPD 5'UTR:YFP:Tnos (GB1483)

   Plasmid

   #160557

   Purpose

   Part 3/3 for assembly of site-specific recombination based Registers. Composed of the 5'UTR sequence of 35S promoter, coding sequence of YFP and NOS terminator (5'UTR:YFP:TNos)

   Depositor

   Diego Orzaez

   Article

   Bernabe-Orts et al Nucleic Acids Res. 2020 Apr 6;48(6):3379

   Insert

   5'UTR:YFP:Tnos
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   Available Since

   March 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pUPD2 Assembly Register 3/3 Part - 5'UTR:Luc:Tnos (GB1500)

   Plasmid

   #160581

   Purpose

   Part 3/3 for assembly of site-specific recombination based Registers. Composed of the 5'UTR sequence of 35S promoter, coding sequence of Luc and NOS terminator

   Depositor

   Diego Orzaez

   Article

   Bernabe-Orts et al Nucleic Acids Res. 2020 Apr 6;48(6):3379

   Insert

   Luc
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   Available Since

   Jan. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pEGB3 alpha2 OplexA:mini35S:PhiC31int:Tnos (GB1529)

   Plasmid

   #160614

   Purpose

   TU for the regulated expression of the PhiC31 phage integrase gene driven by a synthetic promoter consisting of the LexA operator fused to the minimal 35S promoter.

   Depositor

   Diego Orzaez

   Article

   Bernabe-Orts et al Nucleic Acids Res. 2020 Apr 6;48(6):3379

   Insert

   PhiC31
   

   Use

   Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   mini35s

   Available Since

   Jan. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pEGB_3alpha1 OplacI:mini35S:phiC31:Tnos (GB1530)

   Plasmid

   #160615

   Purpose

   TU for the regulated expression of the PhiC31 phage integrase gene driven by a synthetic promoter consisting of the LacI operator fused to the minimal 35S promoter.

   Depositor

   Diego Orzaez

   Article

   Bernabe-Orts et al Nucleic Acids Res. 2020 Apr 6;48(6):3379

   Insert

   PhiC31
   

   Use

   Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   mini35s

   Available Since

   Jan. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAS_4xhyb PylT A41AA C55A LaG17-SynNotch 204TAA 442TAG

   Plasmid

   #154778

   Purpose

   plasmid with 4xhybrid PylT cassette (mutant A41AA C55A) and LaG17-SynNotch 204TAA 277TAG, for transient transfection or stable, piggybac-mediated, integration

   Depositor

   Simon Elsaesser

   Article

   Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi

   Insert

   LaG17-SynNotch
   

   Use

   Tags

   LaG17

   Expression

   Mammalian

   Mutation

   204TAA 442TAG in LaG17-SynNotch, hybrid PylT with…

   Promoter

   EF1

   Available Since

   Oct. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pKI-cjACTB-EGFP-3'short

    Plasmid

    #129749

    Purpose

    Gene targeting in marmoset cells

    Depositor

    Hideyuki Okano

    Article

    Yoshimatsu et al Sci Rep. 2019 Feb 6;9(1):1528. doi: 10.1

    Insert

    ACTB (ACTB C. jacchus (common marmost))
    

    Use

    Marmoset targeting

    Tags

    EGFP

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pKI-cjACTB-EGFP-5'short

    Plasmid

    #129748

    Purpose

    Gene targeting in marmoset cells

    Depositor

    Hideyuki Okano

    Article

    Yoshimatsu et al Sci Rep. 2019 Feb 6;9(1):1528. doi: 10.1

    Insert

    ACTB (ACTB C. jacchus (common marmost))
    

    Use

    Marmoset targeting

    Tags

    EGFP

    Expression

    Mutation

    Promoter

    Available Since

    Oct. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pKI-cjACTB-EGFP

    Plasmid

    #129747

    Purpose

    Gene targeting in marmoset cells

    Depositor

    Hideyuki Okano

    Article

    Yoshimatsu et al Sci Rep. 2019 Feb 6;9(1):1528. doi: 10.1

    Insert

    ACTB (ACTB C. jacchus (common marmost))
    

    Use

    Marmoset targeting

    Tags

    EGFP

    Expression

    Mutation

    Promoter

    Available Since

    Oct. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pFL His-I3 V41A / W43A

    Plasmid

    #113515

    Purpose

    Expression of His-I3 V41A / W43 A in insect cells (Baculovirus system)

    Depositor

    Hemmo Meyer

    Article

    Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

    Insert

    I3 (PPP1R11 Human)
    

    Use

    Tags

    6*His

    Expression

    Insect

    Mutation

    V41A /W43A

    Promoter

    Available Since

    Oct. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. GPR152-Tango

    Plasmid

    #66328

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    GPR152 (GPR152 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

15. pMS22H-IRE

    Plasmid

    #133905

    Purpose

    Positive control when used in combination with pAWH-IRP. Expression of MS2BS-IRE site hybrid. Homology regions for recombination with pAWH

    Depositor

    Sebastian Maurer

    Article

    Yang et al Nat Commun. 2018 Sep 14;9(1):3747. doi:

    Insert

    IRE (Ftl1 Rat)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Pol III

    Available Since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLV-CMV-EGFP-PolB-Hygro

    Plasmid

    #176056

    Purpose

    EGFP fused to the N-terminus of PolB & a hygromycin resistance cassette

    Depositor

    Robert Sobol

    Article

    Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

    Insert

    PolB (POLB Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. GPER-Tango

    Plasmid

    #66299

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    GPER (GPER1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. 3xFlag-eGFP-Flag-SETX

    Plasmid

    #218838

    Purpose

    Mammalian expression of GFP-tagged SETX construct for subcellular localization studies with GFP, or immunoprecipitation with Flag tag.

    Depositor

    Albert La Spada

    Article

    Bennett et al Heliyon. 2020 Jun 13;6(6):e04165. doi: 1

    Insert

    Senataxin (SETX Human)
    

    Use

    Tags

    Flag and eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. mCherry-PARP1

    Plasmid

    #215136

    Purpose

    Expresses mCherry-PARP1 in mammalian cells from a retroviral vector.

    Depositor

    Michele Pagano

    Article

    Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

    Insert

    PARP1 (PARP1 Human)
    

    Use

    Retroviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLVX-NLS-HA-TurboID

    Plasmid

    #215075

    Purpose

    Expresses NLS-HA-TurboID in mammalian cells from a lentiviral vector.

    Depositor

    Michele Pagano

    Article

    Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

    Insert

    TurboID (birA )
    

    Use

    Lentiviral

    Tags

    NLS-HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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