We narrowed to 15,732 results for: LEA

1. pSCL.180

   Plasmid

   #185001

   Purpose

   Express -Eco1 AAVS1 editing ncRNA and gRNA

   Depositor

   Seth Shipman

   Article

   Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

   Insert

   Eco1: AAVS1 targeting and editing ncRNA-gRNA (a1/a2 length: 27 v1), expressed constitutively from a pol III (H1) promoter
   

   Tags

   SV40NLS

   Expression

   Mammalian

   Mutation

   AAVS1 donor, a1/a2 length extended for 27 bp

   Promoter

   H1

   Available Since

   Nov. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. GFP-LacI-HIPK2

   Plasmid

   #179269

   Purpose

   Expresses GFP-LacI-HIPK2 fusion protein

   Depositor

   Lienhard Schmitz

   Article

   Haas et al Front Cell Dev Biol. 2020 Sep 1;8:852. d

   Insert

   homeodomain interacting protein kinase 2 (HIPK2 Human)
   

   Tags

   GFP-LacI

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. HS-EB3-GFP

   Plasmid

   #105948

   Purpose

   heat shock inducible transgenesis

   Depositor

   Caren Norden

   Article

   Strzyz et al Dev Cell. 2015 Jan 26;32(2):203-19. doi:

   Insert

   EB3 (MAPRE3 Human)
   

   Tags

   EGFP

   Expression

   Bacterial

   Available Since

   Feb. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pUB-nSypHer (NLS:sypHer:NLS)

   Plasmid

   #84731

   Purpose

   Expresses sypHer in plant cells-biosensor for pH targeted to Nuclei

   Depositor

   Philip Mullineaux

   Article

   Exposito-Rodriguez et al Nat Commun. 2017 Jun 29;8(1):49. doi: 10

   Insert

   nSypHer
   

   Tags

   NLS (nuclear localisation signal and NLS (nuclear…

   Expression

   Plant

   Promoter

   ubiqutin- 10 gene promoter (pUBQ10) of Arabidopsis

   Available Since

   July 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV_Anc689-nCas9

   Plasmid

   #163526

   Purpose

   Mammalian CG-to-GC base editing

   Depositor

   David Liu

   Article

   Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

   Insert

   Anc689-nCas9
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   nCas9 (D10A)

   Available Since

   Dec. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. VVT1

   Plasmid

   #65779

   Purpose

   The Joung Lab recommends using BPK2660 (Addgene plasmid 70709) instead of VVT1 as guide RNAs cloned into BPK2660 are more effective than this plasmid. Human expression plasmid for S. aureus Cas9 sgRNA

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

   Insert

   SaCas9 gRNA backbone, without spacer sequence
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   June 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLV Tmbim6-V5

   Plasmid

   #175150

   Purpose

   Lentiviral expression of mouse Tmbim6-V5

   Depositor

   Larry Gerace

   Article

   Cheng et al Life Sci Alliance. 2023 Jul 11;6(9):e202

   Insert

   Tmbim6 (Tmbim6 Mouse)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Available Since

   Jan. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLV Tmem9-V5

   Plasmid

   #175151

   Purpose

   Lentiviral expression of mouse Tmem9-V5

   Depositor

   Larry Gerace

   Article

   Cheng et al Life Sci Alliance. 2023 Jul 11;6(9):e202

   Insert

   Tmem9 (Tmem9 Mouse)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Available Since

   Jan. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. shPBRM1 #122-Tet-pLKO-puro

   Plasmid

   #107401

   Purpose

   Lentiviral expression of shRNA targeting PBRM

   Depositor

   William Kaelin

   Article

   Gao et al Proc Natl Acad Sci U S A. 2017 Jan 31;11

   Insert

   shRNA targeting PBRM1 (PBRM1 Human)
   

   Use

   Lentiviral

   Available Since

   June 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNAintron-CHIKV-nsp1-c3xFLAG

    Plasmid

    #215694

    Purpose

    Produces the Chikungunya virus nonstructural protein 1 with a c-terminal 3xFLAG tag

    Depositor

    Melinda Brindley

    Article

    Reyes Ballista et al J Virol. 2024 Jul 15:e0077524. doi: 10.1

    Insert

    CHIKV nsp1
    

    Tags

    3xFLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 1, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pcDNAintron-CHIKV-nsp2-c3xFLAG

    Plasmid

    #215695

    Purpose

    Produces the Chikungunya virus nonstructural protein 2 with a c-terminal 3xFLAG tag

    Depositor

    Melinda Brindley

    Article

    Reyes Ballista et al J Virol. 2024 Jul 15:e0077524. doi: 10.1

    Insert

    CHIKV nsp2
    

    Tags

    3xFLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 1, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pcDNAintron-CHIKV-nsp4-c3xFLAG

    Plasmid

    #215697

    Purpose

    Produces the Chikungunya virus nonstructural protein 4 with a c-terminal 3xFLAG tag

    Depositor

    Melinda Brindley

    Article

    Reyes Ballista et al J Virol. 2024 Jul 15:e0077524. doi: 10.1

    Insert

    CHIKV nsp4
    

    Tags

    3xFLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 1, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. HOXA10-MSCV

    Plasmid

    #20980

    Depositor

    Corey Largman

    Article

    Thorsteinsdottir et al Mol Cell Biol. 1997 Jan . 17(1):495-505.

    Insert

    HOXA10 (HOXA10 Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    Aug. 14, 2009

    Availability

    Academic Institutions and Nonprofits only

14. mCherry-YOD1

    Plasmid

    #85663

    Purpose

    Expression of human YOD1 with N-terminal mCherry tag

    Depositor

    Hemmo Meyer

    Article

    Papadopoulos et al EMBO J. 2016 Oct 17. pii: e201695148.

    Insert

    YOD1 (YOD1 Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRSFDuet-1_VNp6-StefinA

    Plasmid

    #182404

    Purpose

    Bacterial expression of Vesicle Nucleating peptide6-stefinA fusion

    Depositor

    Dan Mulvihill

    Article

    Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

    Insert

    VNp6-stefin (CSTA Human)
    

    Tags

    Vesicle Nucleating peptide 6 (VNp6)

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pIS1 DICER1 long UTR

    Plasmid

    #21649

    Depositor

    David Bartel

    Article

    Mayr et al Cell. 2009 Aug 21. 138(4):673-84.

    Insert

    Dicer 1 long UTR (DICER1 Human)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Available Since

    July 22, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHS0667 KraIscB-1 non-coding locus in pCOLADuet-1

    Plasmid

    #176537

    Purpose

    Non-protein coding locus components from KraIscB-1

    Depositor

    Feng Zhang

    Article

    Altae-Tran et al Science. 2021 Oct;374(6563):57-65. doi:

    Insert

    non-coding locus components from KraIscB-1 locus
    

    Expression

    Bacterial

    Available Since

    Nov. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. mADAM10 deltaMP in pQCXIP cFLAG

    Plasmid

    #19138

    Depositor

    Zena Werb

    Article

    Lemieux et al J Biol Chem. 2007 May 18. 282(20):14836-

    Insert

    ADAM10 (Adam10 Mouse)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    delta 56-457

    Available Since

    Sept. 8, 2008

    Availability

    Academic Institutions and Nonprofits only

19. pTU387

    Plasmid

    #178981

    Purpose

    pACYC Duet-1 containing full Type III-E system Candidatus “Scalindua brodae” CRISPR array

    Depositor

    Stan Brouns

    Article

    van Beljouw et al Science. 2021 Sep 17;373(6561):1349-1353

    Insert

    CRISPR array from Candidatus Scalindua brodae
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMV_APOBEC1-UdgX-nCas9

    Plasmid

    #163531

    Purpose

    Mammalian CG-to-GC base editing

    Depositor

    David Liu

    Article

    Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

    Insert

    APOBEC1-UdgX-nCas9
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    nCas9 (D10A)

    Available Since

    Jan. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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