We narrowed to 14,477 results for: NTS;

1. pCMV-eNme2-C.NR

   Plasmid

   #185672

   Purpose

   Expresses nuclease active eNme2-C.NR in mammalian cells

   Depositor

   David Liu

   Article

   Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038

   Insert

   eNme2-C.NR nuclease
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   eNme2-C S6P/G33E/A520E/S646R/V696F/R711G/V758I/Y7…

   Available Since

   Sept. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. KHBD00727

   Plasmid

   #39748

   Depositor

   Bart Deplancke

   Article

   Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

   Insert

   CG9932 (CG9932 Fly)
   

   Use

   Gateway donr vector

   Promoter

   none

   Available Since

   Sept. 18, 2012

   Availability

   Academic Institutions and Nonprofits only

3. pDIRECT_10A

   Plasmid

   #91207

   Purpose

   Direct Cloning, Type: non-T-DNA, Engineering Reagent: 35S:AtCas9 + AtU6:gRNA, Plant Selection: none

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   35S:AtCas9 + AtU6:gRNA
   

   Use

   CRISPR

   Expression

   Plant

   Available Since

   July 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGWB455

   Plasmid

   #74837

   Purpose

   Gateway cloning compatible binary vector for N-terminal fusion with mRFP (CaMV35S promoter).

   Depositor

   Tsuyoshi Nakagawa

   Article

   Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

   Type

   Empty backbone

   Expression

   Plant

   Promoter

   CaMV35S

   Available Since

   Sept. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDECKO_GFP

   Plasmid

   #72619

   Purpose

   Expresses two gRNAs targeting GFP

   Depositor

   Roderic Guigo, Rory Johnson

   Article

   Aparicio-Prat et al BMC Genomics. 2015 Oct 23;16(1):846. doi

   Insert

   gRNAs toward GFP
   

   Expression

   Mammalian

   Promoter

   U6 (gRNA1) and H1 (gRNA2)

   Available Since

   June 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCW57-GFP-2A-Cas9

   Plasmid

   #170805

   Purpose

   All-in-one doxycycline inducible lentiviral vector for expression of Cas9 in combination with turbo GFP using the P2A self-cleaving peptide

   Depositor

   Morten Frodin

   Article

   Niu et al Nat Genet. 2022 Dec 5. doi: 10.1038/s415

   Insert

   Cas9
   

   Use

   CRISPR and Lentiviral

   Tags

   NLS

   Expression

   Mammalian

   Available Since

   Nov. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pZS157 CRISPEY RT/Cas9

   Plasmid

   #114454

   Purpose

   Yeast Integration Plasmid for galactose inducible Ec86-RT and SpCas9 expression

   Depositor

   Hunter Fraser

   Article

   Sharon et al Cell. 2018 Sep 18. pii: S0092-8674(18)31

   Insert

   SpCas9, Ec86-RT
   

   Use

   CRISPR

   Tags

   SV40-NLS

   Expression

   Yeast

   Mutation

   S. cerevisiae codon optimized

   Promoter

   GAL1-GAL10

   Available Since

   Oct. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGWB404

   Plasmid

   #74798

   Purpose

   Gateway cloning compatible binary vector for C-terminal fusion with sGFP (no promoter).

   Depositor

   Tsuyoshi Nakagawa

   Article

   Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

   Type

   Empty backbone

   Expression

   Plant

   Promoter

   No Promoter

   Available Since

   July 18, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1-hAsCpf1(TYCV) (pY210)

   Plasmid

   #89351

   Purpose

   Expresses humanized AsCpf1 variant that recognizes TYCV PAMs

   Depositor

   Feng Zhang

   Article

   Gao et al Nat Biotechnol. 2017 Jun 5. doi: 10.1038

   Insert

   Cpf1, RR variant
   

   Use

   CRISPR

   Tags

   NLS and NLS-3xHA

   Expression

   Mammalian

   Mutation

   S542R/K607R

   Promoter

   CMV

   Available Since

   June 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pmCherry_sgRNA-ver2

    Plasmid

    #126776

    Purpose

    gRNA expression vector with mCherry transfection control, does not contain the direct repeat of the truncated guideRNA

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

    Insert

    sgRNA (for SpCas9)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pK7WG2-mitoTAL2-orf125

    Plasmid

    #158737

    Purpose

    Plant mitoTALEN vector to cut Brassica CMS gene orf125 (orf138)

    Depositor

    Shin-ichi Arimura

    Article

    Kazama et al Nat Plants. 2019 Jul;5(7):722-730. doi:

    Insert

    mitoTALEN left and right to target orf125 (orf138)
    

    Use

    TALEN

    Available Since

    Oct. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. phINA

    Plasmid

    #132606

    Purpose

    Expresses human alpha-internexin

    Depositor

    Anthony Brown

    Article

    Stone et al Cytoskeleton (Hoboken). 2019 Jul;76(7-8)

    Insert

    INA (INA Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pACS145

    Plasmid

    #204600

    Purpose

    Histone H3.3K36M-V5

    Depositor

    Tom Misteli

    Article

    Schibler et al Elife. 2023 May 23;12:e80653. doi: 10.75

    Insert

    histone H3.3 K36M (H3-3A Human)
    

    Use

    Lentiviral

    Tags

    human histone H3.3 K36M - V5

    Expression

    Mammalian

    Mutation

    histone H3.3 K36M

    Promoter

    CMV

    Available Since

    Jan. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDGE337

    Plasmid

    #153246

    Purpose

    M4E shuttle vector containing the Arabidopsis thaliana U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

    Depositor

    Johannes Stuttmann

    Article

    Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. AD59_pEP.DonorCLYBL.TS

    Plasmid

    #199227

    Purpose

    Donor plasmid with CLYBL target sites for ITPN or HMEJ knock-in at the human CLYBL safe harbor locus

    Depositor

    Manuel Goncalves

    Article

    Wang et al Nucleic Acids Res. 2023 Mar 17:gkad165.

    Insert

    Expression unit for PuroR.T2A.EGFP selectable and reporter markers
    

    Use

    Gene targeting donor plasmid

    Expression

    Mammalian

    Promoter

    Human PGK1 gene promoter

    Available Since

    April 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGWB418

    Plasmid

    #74812

    Purpose

    Gateway cloning compatible binary vector for N-terminal fusion with 4xMyc (CaMV35S promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Expression

    Plant

    Promoter

    CaMV35S

    Available Since

    Sept. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGWB515

    Plasmid

    #74857

    Purpose

    Gateway cloning compatible binary vector for N-terminal fusion with 3xHA (CaMV35S promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Expression

    Plant

    Promoter

    CaMV35S

    Available Since

    Aug. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. Luc-noPS

    Plasmid

    #177940

    Purpose

    Transient mammalian expression of Luc2 (firefly luciferase). Lacks packaging sequence and is used as a negative control transfer plasmid for SARS-CoV-2 virus-like particles.

    Depositor

    Jennifer Doudna

    Article

    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

    Insert

    Luc2
    

    Expression

    Mammalian

    Available Since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGWB442

    Plasmid

    #74828

    Purpose

    Gateway cloning compatible binary vector for N-terminal fusion with eYFP (CaMV35S promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Expression

    Plant

    Promoter

    CaMV35S

    Available Since

    July 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pRK5-hGRK4

    Plasmid

    #32690

    Depositor

    Robert Lefkowitz

    Article

    Premont et al J Biol Chem. 1996 Mar 15;271(11):6403-10

    Insert

    GRK4 (GRK4 Human)
    

    Expression

    Mammalian

    Available Since

    Feb. 21, 2012

    Availability

    Academic Institutions and Nonprofits only