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PCUP1-DuDre-Atg8-404

Plasmid

#69201

Purpose

RFP-Atg8 (PAS marker) with improved property

Depositor

Zhiping Xie

Article

Li et al Autophagy. 2015;11(6):954-60. doi: 10.10

Insert

DuDre-Atg8

Use

Tags

DsRed Express 2

Expression

Yeast

Mutation

Promoter

Available since

Sept. 28, 2015

Availability

Academic Institutions and Nonprofits only

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pBRC169

Plasmid

#79670

Purpose

DsREDmonomer-based BiFC vector for transient expression of RC (169-225) in plants.

Depositor

Yutaka Kodama, Masamitsu Wada

Article

Kodama et al Plant Mol Biol. 2009 May;70(1-2):211-7.

Type

Empty backbone

Use

Reporter plasmid

Tags

DsRED-monomer (169–225)/RC169

Expression

Plant

Mutation

Promoter

CaMV35S

Available since

Aug. 18, 2016

Availability

Academic Institutions and Nonprofits only

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pBRN168

Plasmid

#79668

Purpose

DsREDmonomer-based BiFC vector for transient expression of RN (1-168) in plants.

Depositor

Yutaka Kodama, Masamitsu Wada

Article

Kodama et al Plant Mol Biol. 2009 May;70(1-2):211-7.

Type

Empty backbone

Use

Reporter plasmid

Tags

DsRED-monomer (1–168)/RN168

Expression

Plant

Mutation

Promoter

CaMV35S

Available since

Dec. 2, 2016

Availability

Academic Institutions and Nonprofits only

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OA-986B

Plasmid

#124999

Purpose

Expresses dCas9-VPR driven by the Ubiquitin-63E promoter

Depositor

Omar Akbari

Article

Buchman et al Nat Commun. 2021 Jun 2;12(1):3281. doi:

Insert

dCas9-VPR

Use

Piggyback

Tags

Expression

Insect

Mutation

Promoter

Ubiquitin

Available since

Oct. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pUASt-ssBiP-ESCargo

Plasmid

#140166

Purpose

Expresses a regulatable secretory cargo for Drosophila melanogaster

Depositor

Benjamin Glick

Article

Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

Insert

ssBip-APVNTT-DsRed-Express2-FKBP(LV)(C22V)

Use

Tags

Expression

Insect

Mutation

Promoter

hs70

Available since

March 26, 2020

Availability

Academic Institutions and Nonprofits only

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OA-986C

Plasmid

#125000

Purpose

Expresses dCas9-VPR driven by the bottleneck promoter

Depositor

Omar Akbari

Article

Buchman et al Nat Commun. 2021 Jun 2;12(1):3281. doi:

Insert

dCas9-VPR

Use

Piggyback

Tags

Expression

Insect

Mutation

Promoter

bottleneck

Available since

Oct. 23, 2020

Availability

Academic Institutions and Nonprofits only

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OA-986D

Plasmid

#125001

Purpose

Expresses dCas9-VP64 driven by the Ubiquitin-63E promoter

Depositor

Omar Akbari

Article

Buchman et al Nat Commun. 2021 Jun 2;12(1):3281. doi:

Insert

dCas9-VP64

Use

Piggyback

Tags

Expression

Insect

Mutation

Promoter

ubiquitin

Available since

Oct. 23, 2020

Availability

Academic Institutions and Nonprofits only

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OA-986E

Plasmid

#125002

Purpose

Expresses dCas9-VP64 driven by the bottleneck promoter

Depositor

Omar Akbari

Article

Buchman et al Nat Commun. 2021 Jun 2;12(1):3281. doi:

Insert

dCas9-VP64

Use

Piggyback

Tags

Expression

Insect

Mutation

Promoter

bottleneck

Available since

Oct. 23, 2020

Availability

Academic Institutions and Nonprofits only

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p160Tol2-her4.3:switchCas9

Plasmid

#227680

Purpose

Expression of cre inducible and her4.3 (Notch) dependent Cas9-GFP and U6-driven 4 sgRNAs for SABER-seq in zebrafish

Depositor

Bushra Raj

Article

Siniscalco et al Development. 2024 Nov 22:dev.203102. doi

Inserts

loxP-dsRed-loxP-Cas9-t2A-GFP
4xU6:sgRNA

Use

CRISPR

Tags

Expression

Mutation

Promoter

U6 and her4.3

Available since

March 4, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3-ClopHensor-H148G-V224L

Plasmid

#46366

Depositor

Daniele Arosio

Article

Mukhtarov et al Front Mol Neurosci. 2013 Apr 18;6:9. doi

Insert

ClopHensor-H148G-V224L

Use

Tags

Strep-II

Expression

Mammalian

Mutation

E2GFP-H148G-V224L is EGFP-T203Y-H148G-V224L

Promoter

Available since

Aug. 12, 2013

Availability

Academic Institutions and Nonprofits only
pDSAR

Plasmid

#62292

Purpose

docking site transgenesis plasmid for GoldenGate Cloning and insect transgenesis, DsRed RFP marker

Depositor

Eric Marois

Article

Volohonsky et al G3 (Bethesda). 2015 Apr 13. pii: g3.115.

Type

Empty backbone

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 23, 2015

Availability

Academic Institutions and Nonprofits only

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pHD-mW-attPCC-FRT (pTL717)

Plasmid

#115158

Purpose

Vector for generating dsDNA donors for HDR. Contains an attP-CC docking site, the visible GMR-white for identifying backbone incorporation flanked by FRT sites for FLP-mediated removal.

Depositor

Tobias Langenhan

Article

Blanco-Redondo et al G3 (Bethesda). 2018 Jul 31. pii: g3.118.

Insert

mW attP-CC FRT

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Oct. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pDSARN

Plasmid

#62295

Purpose

docking site transgenesis plasmid for GoldenGate Cloning and insect transgenesis, DsRed-NLS marker

Depositor

Eric Marois

Article

Volohonsky et al G3 (Bethesda). 2015 Apr 13. pii: g3.115.

Type

Empty backbone

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 23, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV-fSST-RFP

Plasmid

#22913

Depositor

Edward Callaway

Article

Nathanson et al Front Neural Circuits. 2009 . 3():19.

Insert

fugu somatostatin promoter driving DsRed 2

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 25, 2010

Availability

Academic Institutions and Nonprofits only

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pIRES RED/SP M87C448Y

Plasmid

#89324

Purpose

Expression of RFP and M87C448Y spastin in mammalian cells

Depositor

Peter Baas

Article

Solowska et al J Neurosci. 2014 Jan 29;34(5):1856-67. d

Insert

spastin (SPAST Human)

Use

Tags

Expression

Mammalian

Mutation

1-460 deleted

Promoter

CMV

Available since

April 11, 2017

Availability

Academic Institutions and Nonprofits only

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Ubiq-Cas9.874W

Plasmid

#112686

Purpose

Express hSpCas9 under Ubiquitin-63E promoter

Depositor

Omar Akbari

Article

Kandul et al Nat Commun. 2019 Jan 8;10(1):84. doi: 10

Insert

hSpCas9

Use

Tags

3x FLAG, EGFP, and NLS

Expression

Insect

Mutation

Humanized Cas9 bacterial sequence

Promoter

Ubiquitin-63E promoter

Available since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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vas-Cas9.874Z

Plasmid

#112687

Purpose

Express hSpCas9 under vasa promoter

Depositor

Omar Akbari

Article

Kandul et al Nat Commun. 2019 Jan 8;10(1):84. doi: 10

Insert

hSpCas9

Use

Tags

3x FLAG, EGFP, and NLS

Expression

Insect

Mutation

Humanized Cas9 bacterial sequence

Promoter

vasa

Available since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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nos-Cas9.874Z1

Plasmid

#112685

Purpose

Express hSpCas9 under nanos promoter

Depositor

Omar Akbari

Article

Kandul et al Nat Commun. 2019 Jan 8;10(1):84. doi: 10

Insert

hSpCas9

Use

Tags

3x FLAG, EGFP, and NLS

Expression

Insect

Mutation

Humanized Cas9 bacterial sequence

Promoter

nanos

Available since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pLVX-Egr2ASRNA

Plasmid

#177737

Purpose

Egr2 AS-RNA lentiviral expression vector

Depositor

Nikolaos Tapinos

Article

Martinez-Moreno et al Cell Rep. 2017 Aug 22;20(8):1950-1963. d

Insert

Egr2-AS-RNA

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

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Opie2-NeoR[traF]+Opie2-PuroR[dsxM]

Plasmid

#131618

Purpose

Expresses a functional NeoR only in females, a functional PuroR only in males, and dsRed in both genders.

Depositor

Omar Akbari

Article

Kandul et al Nat Commun. 2020 Apr 30;11(1):2106. doi:

Inserts

PuroR[dsxM]
NeoR[traF]

Use

Tags

Expression

Insect

Mutation

Promoter

Opie2

Available since

May 7, 2020

Availability

Academic Institutions and Nonprofits only

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