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pSpCas9(BB)-2A-Puro (PX459)
Plasmid#48139PurposeNOTE: A new version of this plasmid is now available. See Addgene plasmid 62988.DepositorInserthSpCas9-2A-Puro
UseCRISPRTags2A-Puro and 3XFLAGExpressionMammalianMutationPromoterCbhAvailable sinceOct. 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
pX097-U6-SpTracrRNA-EF1a-hSpRNaseIII-mCherry
Plasmid#41863PurposeThis plasmid is used to reconstitute the complete Type II CRISPR system from S. pyogenes and contains the host factor RNaseIII and tracrRNA.DepositorArticleInsertU6-SpTracrRNA-EF1a-hSpRNaseIII-mCherry
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 27, 2013AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9n(BB) (PX460)
Plasmid#48873PurposeCas9n (D10A nickase mutant) from S. pyogenesDepositorHas ServiceCloning Grade DNAInserthSpCas9n
UseCRISPRTags3XFLAGExpressionMammalianMutationCas9 D10A nickase mutantPromoterCbhAvailable sinceOct. 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
2.2
Plasmid#34885DepositorInsertSindbis virus envelope protein
UseLentiviralTagsExpressionMammalianMutationSGN UTR in E1 domainPromoterCMVAvailable sinceSept. 28, 2012AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-EGFP-Rac1-wt
Plasmid#12980DepositorInsertRac1 (RAC1 Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceOct. 27, 2006AvailabilityAcademic Institutions and Nonprofits only -
UbG76V-eGFP-V5His_20aa
Plasmid#23970DepositorInsertUb-G76V-eGFP-V5HIS_20aa
UseTagseGFPExpressionMammalianMutationG76V mutation in Ubiquitin. Ub fused to N-termin…PromoterAvailable sinceMarch 22, 2010AvailabilityAcademic Institutions and Nonprofits only -
human p53-(1-393)-L344P
Plasmid#24862DepositorInsertp53 (TP53 Human)
UseTagsHisExpressionBacterialMutationLeucine 344 mutated to ProlinePromoterAvailable sinceAug. 20, 2010AvailabilityAcademic Institutions and Nonprofits only -
PX429 SpCas9 N863A nickase
Plasmid#68706PurposeSpCas9 N863A nickaseDepositorInsertSpCas9 N863A nicking mutant
UseCRISPRTagsExpressionMammalianMutationPromoterCbhAvailable sinceSept. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
Ub[9]-mRFP[1]
Plasmid#20249DepositorInsertubiquitin C (UBC Human)
UseTagsmRFPExpressionMammalianMutationchanged Stop codon 686 to GlycinePromoterAvailable sinceApril 14, 2009AvailabilityAcademic Institutions and Nonprofits only -
human p53-(1-393)-N268D
Plasmid#24861DepositorInsertp53 (TP53 Human)
UseTagsHisExpressionBacterialMutationAsparagine 268 mutated to AspartatePromoterAvailable sinceAug. 5, 2010AvailabilityAcademic Institutions and Nonprofits only -
human p53-Δ(325-355)
Plasmid#24871DepositorInsertp53 (TP53 Human)
UseTagsHisExpressionBacterialMutationDeletion of aa 325-355PromoterAvailable sinceAug. 20, 2010AvailabilityAcademic Institutions and Nonprofits only -
human p53-(310-360)
Plasmid#24872DepositorInsertp53 (TP53 Human)
UseTagsHisExpressionBacterialMutationContains only the dimeric oligomerization domain …PromoterAvailable sinceAug. 5, 2010AvailabilityAcademic Institutions and Nonprofits only -
pGENE:EFF-1::mCherry::V5
Plasmid#133020PurposeEFF-1 fused to mCherry fluorescent protein cloned into the pGene vector for inducible expression in mammalian cellsDepositorInsertEFF-1::mCherry::V5
UseTagsV5; mCherryExpressionMammalianMutationPromoterAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
human p53-(1-393)-M340Q-L344R
Plasmid#24863DepositorInsertp53 (TP53 Human)
UseTagsHisExpressionBacterialMutationMethionine 340 mutated to Glutamine Leucine 344 …PromoterAvailable sinceAug. 20, 2010AvailabilityAcademic Institutions and Nonprofits only -
PX544 SpCas9(del1098-1368)
Plasmid#58889PurposeTruncated form of SpCas9 without C' domainDepositorInserthSpCas9
UseCRISPRTags3XFLAGExpressionMammalianMutationdel amino acids 1098-1368PromoterAvailable sinceSept. 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pJC-20xUAS-TALE3-VP64-P10
Plasmid#104607PurposeExpresses TALE3 under UAS controlDepositorInsertTALE3
UseTALENTagsExpressionInsectMutationPromoterhsp70Available sinceJan. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pre_IV_CS6_4323_v1
Plasmid#126859Purpose4323 b scaffold from compatibility group 4, made by method IV, insert based on de Bruijn sequence with the an order of 7DepositorInsertIV_CS6_4323_v1
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceOct. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
PX543 SpCas9(del175-307)
Plasmid#58888PurposeTruncated form of SpCas9 without REC2 domainDepositorInserthSpCas9
UseCRISPRTags3XFLAGExpressionMammalianMutationdel amino acids 175-307PromoterAvailable sinceSept. 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pJC-20xUAS-TALE1-VP64-P10
Plasmid#104606PurposeExpresses TALE1 under UAS controlDepositorInsertTALE1
UseTALENTagsExpressionInsectMutationPromoterhsp70Available sinceJan. 29, 2018AvailabilityAcademic Institutions and Nonprofits only
