We narrowed to 2,712 results for: nags

1. pTH1-SaSrtA-K

   Plasmid

   #64978

   Purpose

   Expresses the E105K mutant of Staphylococcus aureus sortase A in E. coli

   Depositor

   Teruyuki Nagamune

   Article

   Hirakawa et al Biotechnol Bioeng. 2012 Dec;109(12):2955

   Insert

   sortase A
   

   Use

   Tags

   His6 tag, Mxe GyrA intein, and chitin-binding pro…

   Expression

   Bacterial

   Mutation

   deleted amino acids 1-59, E105K

   Promoter

   Available since

   June 5, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. Phamret-H2B/pcDNA3

   Plasmid

   #51955

   Purpose

   mammalian expression of Phamret, a monomeric photoconvertible fluorescent protein, fused to H2B

   Depositor

   Takeharu Nagai

   Article

   Matsuda et al Nat Methods. 2008 Apr;5(4):339-45. doi:

   Insert

   Phamret
   

   Use

   Tags

   human H2B

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 15, 2014

   Availability

   Academic Institutions and Nonprofits only

3. Phamret-Fibrillarin/pcDNA3

   Plasmid

   #51954

   Purpose

   mammalian expression of Phamret, a monomeric photoconvertible fluorescent protein, fused to Fibrillarin for nucleolar localization

   Depositor

   Takeharu Nagai

   Article

   Matsuda et al Nat Methods. 2008 Apr;5(4):339-45. doi:

   Insert

   Phamret
   

   Use

   Tags

   Fibrillarin

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 15, 2014

   Availability

   Academic Institutions and Nonprofits only

4. PX333-T2A-EGFP

   Plasmid

   #197417

   Purpose

   SpCas9-T2A-EGFP with dual sgRNA cloning backbones

   Depositor

   Yeh-Hsing Lao

   Article

   Yoshinaga et al Nano Lett. 2023 Jan 17. doi: 10.1021/acs

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CBh; U6

   Available since

   March 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. mtsCOXVIIIx2-B-gTEMP/pcDNA3

   Plasmid

   #199806

   Purpose

   Fluorescent temperature indicator B-gTEMP fused with mitochondrial targeting sequence

   Depositor

   Takeharu Nagai

   Article

   Lu et al Nano Lett. 2022 Jul 27;22(14):5698-5707.

   Insert

   B-gTEMP
   

   Use

   Tags

   mtsCOXVIIIx2

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. SPDK3959

   Plasmid

   #199246

   Purpose

   Modified TRV RNA2 with PEBV promoter and AtIleu-tRNA vector with AtPDS3 target for editing Arabidopsis PDS3 gene

   Depositor

   Savithramma Dinesh-Kumar

   Article

   Nagalakshmi et al Plant Physiol. 2022 Jun 27;189(3):1241-1

   Insert

   Modified TRV RNA2 with PEBV promoter, AtPDS3 target and AtIleu-tRNA mobile RNA sequence
   

   Use

   T-dna vector

   Tags

   Expression

   Mutation

   Promoter

   PEBV

   Available since

   June 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTH1-SaSrtA

   Plasmid

   #64973

   Purpose

   Expresses the wild type of Staphylococcus aureus sortase A in E. coli

   Depositor

   Teruyuki Nagamune

   Article

   Hirakawa et al Biotechnol Bioeng. 2012 Dec;109(12):2955

   Insert

   sortase A
   

   Use

   Tags

   His6 tag, Mxe GyrA intein, and chitin-binding pro…

   Expression

   Bacterial

   Mutation

   deleted amino acids 1-59

   Promoter

   T7 promoter

   Available since

   July 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. PB-TET

   Plasmid

   #20909

   Purpose

   piggyBac empty vector with Gateway cassette, tet inducible promoter, and linked reporter (IRES-bgeo)

   Depositor

   Andras Nagy

   Article

   Woltjen et al Nature. 2009 Apr 9;458(7239):766-70. doi

   Insert

   Gateway Destination Vector
   

   Use

   piggybac

   Tags

   IRES-bGeo

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 5, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. CAG FUCCI

   Plasmid

   #175266

   Purpose

   Bicistronic FUCCI construct driven by a CAG promoter. Contains I-SceI sites for transgenesis

   Depositor

   James Monaghan

   Article

   Duerr et al Development. 2022 Mar 15;149(6). pii: 27

   Insert

   FUCCI
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Sept. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pEFmEGFP-mDia2

    Plasmid

    #25407

    Depositor

    Arthur Alberts

    Article

    Tominaga et al Mol Cell. 2000 Jan . 5(1):13-25.

    Insert

    mDia2 (Diaph3 Mouse)
    

    Use

    Tags

    EGFP, His, and Myc

    Expression

    Mammalian

    Mutation

    Encodes mDia2

    Promoter

    Available since

    Aug. 24, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. Nano-lantern/pcDNA3

    Plasmid

    #51970

    Purpose

    mammalian expression of Nano-lantern, a luminescent protein for high-speed single-cell and whole body imaging

    Depositor

    Takeharu Nagai

    Article

    Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

    Insert

    Nano-lantern
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    S257G in Rluc8

    Promoter

    Available since

    March 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. 35S::GeNL/pCAMBIA1301

    Plasmid

    #190070

    Purpose

    Expressing bioluminescent pH indicator-GeNL- in Arabidopsis thaliana

    Depositor

    Takeharu Nagai

    Article

    Tran et al Plant Cell Environ. 2022 Jul 21. doi: 10

    Insert

    Green-enhanced Nano-lantern
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    CaMV 35S

    Available since

    April 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. Yellow Cameleon-Nano65/pcDNA3

    Plasmid

    #51965

    Purpose

    mammalian expression of Yellow Cameleon-Nano65, an ultrasensitive Ca2+ indicator with Kd = 65 nM

    Depositor

    Takeharu Nagai

    Article

    Horikawa et al Nat Methods. 2010 Sep;7(9):729-32. doi:

    Insert

    Yellow Cameleon-Nano65
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 15, 2014

    Availability

    Academic Institutions and Nonprofits only

14. Sirius-mT-Sapphire_pcDNA3

    Plasmid

    #89584

    Purpose

    Genetically encoded ratiometric fluorescent thermometer

    Depositor

    Takeharu Nagai

    Article

    Nakano et al PLoS One. 2017 Feb 17;12(2):e0172344. do

    Insert

    Sirius-mT-Sapphire
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Nano-lantern(cAMP-1.6)/pRSETB

    Plasmid

    #53591

    Purpose

    Nano-lantern-based luminescent cAMP indicator

    Depositor

    Takeharu Nagai

    Article

    Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

    Insert

    Nano-lantern-based luminescent cAMP indicator
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 9, 2014

    Availability

    Academic Institutions and Nonprofits only

16. Yellow Cameleon-Nano15/pcDNA3

    Plasmid

    #51961

    Purpose

    mammalian expression of Yellow Cameleon-Nano15, an ultrasensitive Ca2+ indicator with Kd = 15 nM

    Depositor

    Takeharu Nagai

    Article

    Horikawa et al Nat Methods. 2010 Sep;7(9):729-32. doi:

    Insert

    Yellow Cameleon-Nano15
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 15, 2014

    Availability

    Academic Institutions and Nonprofits only

17. Nano-lantern(cAMP-1.6)/pcDNA3

    Plasmid

    #53594

    Purpose

    Nano-lantern-based luminescent cAMP indicator

    Depositor

    Takeharu Nagai

    Article

    Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

    Insert

    Nano-lantern-based luminescent cAMP indicator
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 10, 2014

    Availability

    Academic Institutions and Nonprofits only

18. Nano-lantern/pRSETB

    Plasmid

    #51969

    Purpose

    bacterial expression of Nano-lantern, a luminescent protein for high-speed single-cell and whole body imaging

    Depositor

    Takeharu Nagai

    Article

    Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

    Insert

    Nano-lantern
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    S257G in Rluc8

    Promoter

    Available since

    March 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pSN007

    Plasmid

    #102440

    Purpose

    For PCR amplification to create paired gRNAs to clone into Golden Gate gRNA expression vectors (e.g. pSB700 Plasmid #64046)

    Depositor

    George Church, Alejandro Chavez

    Article

    Nageshwaran et al J Vis Exp. 2018 Oct 2;(140). doi: 10.379

    Insert

    7SK paired gRNA insert
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. LAMP-CeNL_pcDNA3

    Plasmid

    #89514

    Purpose

    Cyan color variant of bright luminescent protein, fused to Lysosomal-associated membrane protein 1

    Depositor

    Takeharu Nagai

    Article

    Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

    Insert

    CeNL
    

    Use

    Tags

    LAMP1

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge