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pX330_H1.2

Plasmid

#247480

Purpose

Expresses SpCas9 and a sgRNA targeting the human histone H1.2 loci for knock-in.

Depositor

Kazuhiro Maeshima

Article

Shimazoe et al Sci Adv. 2026 Apr 10;12(15):eaec9801. do

Insert

H1.2 (HIST1H1C) (H1-2 Human)

Use

CRISPR

Promoter

U6

Available Since

April 13, 2026

Availability

Academic Institutions and Nonprofits only

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pGEM_H1.2_HaloTag

Plasmid

#247481

Purpose

CRISPR/Cas9 donor plasmid to tag human histone H1.2 with Halo tag

Depositor

Kazuhiro Maeshima

Article

Shimazoe et al Sci Adv. 2026 Apr 10;12(15):eaec9801. do

Insert

H1.2 (HIST1H1C) (H1-2 Human)

Use

CRISPR

Tags

HaloTag

Available Since

April 13, 2026

Availability

Academic Institutions and Nonprofits only

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pGEM_H1.0_HaloTag

Plasmid

#247483

Purpose

CRISPR/Cas9 donor plasmid to tag human histone H1.0 with Halo tag

Depositor

Kazuhiro Maeshima

Article

Shimazoe et al Sci Adv. 2026 Apr 10;12(15):eaec9801. do

Insert

H1.0 (H1F0) (H1-0 Human)

Use

CRISPR

Tags

HaloTag

Available Since

April 13, 2026

Availability

Academic Institutions and Nonprofits only

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pAAVS-NDi-H1.2_WT-Halo

Plasmid

#247484

Purpose

Expresses H1.2 (WT) by tet-on system

Depositor

Kazuhiro Maeshima

Article

Shimazoe et al Sci Adv. 2026 Apr 10;12(15):eaec9801. do

Insert

H1.2 (HIST1H1C) (H1-2 Human)

Tags

HaloTag

Expression

Mammalian

Promoter

TRE3G

Available Since

April 13, 2026

Availability

Academic Institutions and Nonprofits only

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pGEM_H1.2_mAID_mClover

Plasmid

#247486

Purpose

CRISPR/Cas9 donor plasmid to tag human histone H1.2 with mAID-mClover

Depositor

Kazuhiro Maeshima

Article

Shimazoe et al Sci Adv. 2026 Apr 10;12(15):eaec9801. do

Insert

H1.2 (HIST1H1C) (H1-2 Human)

Use

CRISPR

Tags

mAID and mClover

Available Since

April 13, 2026

Availability

Academic Institutions and Nonprofits only

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hTRAK1(S200A, S201A, S719A, S919A)

Plasmid

#225225

Purpose

Expresses MYC-tagged hTRAK1 (S200A, S201A, S719A, S919A)

Depositor

Thomas Schwarz

Article

Falk et al J Cell Biol. 2026 Apr 6;225(4):e20250102

Insert

trak1 (TRAK1 Human)

Tags

MYC

Expression

Mammalian

Mutation

S200A, S201A, S719A, S919A mutations

Promoter

CMV

Available Since

Feb. 6, 2026

Availability

Academic Institutions and Nonprofits only

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Fhl2-HA-P2A-meGFP(omp25mts)

Plasmid

#225231

Purpose

Construct expressing HA-tagged Fhl2 followed by a P2A ribosome skip sequence and then a mitochondria targeted mEGFP

Depositor

Thomas Schwarz

Article

Falk et al J Cell Biol. 2026 Apr 6;225(4):e20250102

Insert

Fhl2 (Fhl2 Rat)

Tags

HA-P2A-mitomeGFP-

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 6, 2026

Availability

Academic Institutions and Nonprofits only

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pLVX-CAG-Fhl2-HA-P2A-mitomeGFP

Plasmid

#219576

Purpose

Lentiviral construct expressing HA-tagged Fhl2 followed by a P2A ribosome skip sequence and then a mitochondria targeted mEGFP.

Depositor

Thomas Schwarz

Article

Falk et al J Cell Biol. 2026 Apr 6;225(4):e20250102

Insert

Fhl2 (Fhl2 Rat)

Use

Lentiviral

Tags

HA-P2A-mitomeGFP-

Expression

Mammalian

Promoter

CAG

Available Since

Feb. 6, 2026

Availability

Academic Institutions and Nonprofits only

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MYC-hTRAK1(S200A, S201A)

Plasmid

#219572

Purpose

Expresses MYC-tagged hTRAK1 (S200A, S201A)

Depositor

Thomas Schwarz

Article

Falk et al J Cell Biol. 2026 Apr 6;225(4):e20250102

Insert

trak1 (TRAK1 Human)

Tags

MYC

Expression

Mammalian

Mutation

Serine 200 changed to alanine, serine 201 changed…

Promoter

CMV

Available Since

Feb. 6, 2026

Availability

Academic Institutions and Nonprofits only

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MYC-hTRAK1(S200A, S201A, S719A, T834A, S919A)

Plasmid

#219574

Purpose

Expresses MYC-tagged hTRAK1 (S200A, S201A, S719A, T834A, S919A)

Depositor

Thomas Schwarz

Article

Falk et al J Cell Biol. 2026 Apr 6;225(4):e20250102

Insert

trak1 (TRAK1 Human)

Tags

MYC

Expression

Mammalian

Mutation

S200A, S201A, S719A, T834A, S919A mutations

Promoter

CMV

Available Since

Feb. 6, 2026

Availability

Academic Institutions and Nonprofits only

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pCMV-Gpr45-S214P-3xFlag

Plasmid

#245987

Purpose

Express 3xFlag C-terminal tagged mouse GPR45-S214P mutant transiently via transfection in mammalian cells.

Depositor

Zhao Zhang

Article

Xun et al Science. 2025 Jun 5;388(6751):eadp3989.

Insert

Gpr45 (Gpr45 Mouse)

Tags

3xFlag

Expression

Mammalian

Mutation

S214P

Promoter

CMV

Available Since

Jan. 6, 2026

Availability

Academic Institutions and Nonprofits only

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pEF1-EGFP-coilin-FRT

Plasmid

#247448

Purpose

FLP/FRT based mammalian expression vector for EGFP-coilin, driven by EF1α promoter.

Depositor

Kazuhiro Maeshima

Article

Itoh et al Life Sci Alliance. 2021 Feb 3;4(4):e2020

Insert

coilin (COIL) (COIL Human)

Tags

EGFP

Expression

Mammalian

Promoter

EF1α

Available Since

Jan. 6, 2026

Availability

Academic Institutions and Nonprofits only

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pBOB-Adcy3-HA

Plasmid

#245988

Purpose

Express HA C-terminal tagged wild-type mouse ADCY3 transiently via transfection or stably via lentivirus-mediated infection in mammalian cells.

Depositor

Zhao Zhang

Article

Xun et al Science. 2025 Jun 5;388(6751):eadp3989.

Insert

Adcy3 (Adcy3 Mouse)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 5, 2026

Availability

Academic Institutions and Nonprofits only

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pBOB-Adcy3-L278H-3xFlag

Plasmid

#245990

Purpose

Express 3xFlag C-terminal tagged mouse ADCY3-L278H mutant transiently via transfection or stably via lentivirus-mediated infection in mammalian cells.

Depositor

Zhao Zhang

Article

Xun et al Science. 2025 Jun 5;388(6751):eadp3989.

Insert

Adcy3 (Adcy3 Mouse)

Use

Lentiviral

Tags

3xFlag

Expression

Mammalian

Mutation

L278H

Promoter

CMV

Available Since

Jan. 5, 2026

Availability

Academic Institutions and Nonprofits only

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pAAVS1-P-6XPyltRNA-dCas9K1151TAG-IRESPylRS

Plasmid

#248695

Purpose

Expression dead CRISPR/Cas9 with unnatural amino acid incorporated at K1151 residue

Depositor

Shuibing Chen

Article

Zhu et al Proc Natl Acad Sci U S A. 2025 Oct 7;122

Insert

dead CRISPR/Cas9 K1151TAG, MbPylRS

Use

CRISPR

Expression

Mammalian

Promoter

CAG

Available Since

Nov. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pMIHP-16272 Flag.Ollas.V5

Plasmid

#241490

Purpose

Flag.Ollas.V5 Flow Code Plasmid This plasmid is to be used with mRNAs.

Depositor

Adrian Liston

Article

Burton et al Immunity. 2024 Jul 9;57(7):1586-1602.e10

Type

Empty backbone

Use

Retroviral

Tags

Flag, Ollas, and V5

Available Since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pMIHP-16286 HA.Ollas.V5

Plasmid

#241493

Purpose

HA.Ollas.V5 Flow Code Plasmid This plasmid is to be used with mRNAs.

Depositor

Adrian Liston

Article

Burton et al Immunity. 2024 Jul 9;57(7):1586-1602.e10

Type

Empty backbone

Use

Retroviral

Tags

HA, Ollas, and V5

Available Since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pMIHP-16285 HA.Ollas.VSVG

Plasmid

#241504

Purpose

HA.Ollas.VSVG Flow Code Plasmid This plasmid is to be used with mRNAs.

Depositor

Adrian Liston

Article

Burton et al Immunity. 2024 Jul 9;57(7):1586-1602.e10

Type

Empty backbone

Use

Retroviral

Tags

HA, Ollas, and VSVG

Available Since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pEF5-H4(A5,8,12,16)-HaloTag-FRT

Plasmid

#247455

Purpose

Expresses wild-type H4(A5,8,12,16)-Halo under EF1 promoter and can be integrated into FRT site

Depositor

Kazuhiro Maeshima

Article

Semeigazin et al Histochem Cell Biol. 2024 Jul;162(1-2):2

Insert

H4 (H4C1) (H4C1 Human)

Tags

HaloTag

Expression

Mammalian

Mutation

H4(A5,8,12,16) has four mutations in the N-termin…

Promoter

EF1α

Available Since

Nov. 10, 2025

Availability

Academic Institutions and Nonprofits only

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PB-EF1-H4-HaloTag-IRES-Neo

Plasmid

#247450

Purpose

Expresses wild-type H4-Halo under EF1 promoter and can be randomly integrated by PiggyBac transposase

Depositor

Kazuhiro Maeshima

Article

Semeigazin et al Histochem Cell Biol. 2024 Jul;162(1-2):2

Insert

H4 (H4C1) (H4C1 Human)

Tags

HaloTag

Expression

Mammalian

Promoter

EF1α

Available Since

Nov. 10, 2025

Availability

Academic Institutions and Nonprofits only

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