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pAAV-Ef1a-DIO-Pgc-1alpha-EYFP

Plasmid

#241793

Purpose

Expression vector for mouse Pgc-1alpha with EYFP

Depositor

Mary Kay Lobo

Article

Chandra et al Biol Psychiatry. 2017 Apr 1;81(7):564-57

Insert

PGC-1alpha (Ppargc1a Mouse)

Use

AAV

Tags

EYFP

Promoter

Ef1a

Available Since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV_CMV-RFP-p2A-DAAO(R285A)-NES

Plasmid

#238921

Purpose

Expression of mutated DAAO(R285A) with a nuclear export signal and RFP as a reporter in mammalian cells

Depositor

Vsevolod Belousov

Article

Kalinichenko et al Redox Biol. 2023 Apr;60:102604. doi: 10.

Insert

RFP-p2A-DAAO(R285A)-NES

Use

AAV

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn1-DIO-Snca (human A53T)

Plasmid

#214201

Purpose

For Cre-dependent expression of alpha synuclein (human gene containing A53T mutation)

Depositor

Thomas Hnasko

Article

Steinkellner et al Brain. 2022 Apr 29;145(3):879-886. doi:

Insert

Snca (A53T) (SNCA Human)

Use

AAV and Cre/Lox

Expression

Mammalian

Mutation

A53T

Available Since

March 29, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-Hsp1-Hsp2Cas9-P2A-puro

Plasmid

#192131

Purpose

Expresses Hsp1-Hsp2Cas9, cloning backbone for sgRNA and puromycin resistance gene

Depositor

Yongming Wang

Article

Gao et al Mol Ther. 2023 Apr 5;31(4):1177-1187. do

Insert

Hsp1-Hsp2Cas9

Use

AAV

Expression

Mammalian

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-Hsp1-Hsp2Cas9-Y-P2A-puro

Plasmid

#192132

Purpose

Expresses highly specific Hsp1-Hsp2Cas9, cloning backbone for sgRNA and puromycin resistance gene

Depositor

Yongming Wang

Article

Gao et al Mol Ther. 2023 Apr 5;31(4):1177-1187. do

Insert

Hsp1-Hsp2Cas9-Y

Use

AAV

Expression

Mammalian

Mutation

Hsp1-Hsp2Cas9 (Y446A)

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pPROBE'-gfp[AAV]

Plasmid

#40169

Depositor

Steven Lindow

Article

Miller et al Mol Plant Microbe Interact. 2000 Nov;13(

Insert

Promotor probe

Available Since

Dec. 3, 2012

Availability

Academic Institutions and Nonprofits only

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pAAV_CAG(del)--FLEx(loxP)-splitTVA:HA

Plasmid

#248665

Purpose

Helper virus for monosynaptic tracing; Expresses TVA and an HA tag in Cre-expressing cells under the CAG promoter. To be co-injected with Rabies Glycoprotein (G) viruses.

Depositor

Gordon Fishell

Article

Mazo et al Res Sq [Preprint]. 2025 Jun 12:rs.3.rs-6

Insert

TVA

Use

AAV

Tags

HA

Expression

Mammalian

Available Since

March 9, 2026

Availability

Academic Institutions and Nonprofits only

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pAAV-U6-DR30(SapI)-0.5Syn-CasRx-pA

Plasmid

#192492

Purpose

To express CasRX and a gRNA

Depositor

Jonathan Ploski

Article

Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

Insert

CasRx

Use

AAV

Mutation

No

Promoter

0.5Syn

Available Since

Aug. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-intron-DIO-DjCas13d-pA

Plasmid

#192498

Purpose

To express DjCas13d in a Cre dependent manner

Depositor

Jonathan Ploski

Article

Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

Insert

DjCas13d

Use

AAV

Mutation

No

Promoter

CMV

Available Since

Aug. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV TRE-Tight CheRiff-mKate2

Plasmid

#182493

Purpose

Expression of CheRiff channelrhodopsin linked to mKate2 from a Tet-inducible promoter

Depositor

Thomas Oertner

Article

Lamothe-Molina et al Nat Commun. 2022 Oct 26;13(1):6376. doi:

Insert

CheRiff (#51697 Adam Cohen Lab)

Use

AAV

Tags

mKate2 (Evrogen)

Expression

Mammalian

Promoter

TRE-Tight (Clontech)

Available Since

Aug. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1_-DIO-Varnam1-WPRE

Plasmid

#240054

Purpose

Cre dependent Red fluorescent voltage indicator for mammalian expression

Depositor

Mark Schnitzer

Article

Haziza et al Cell, 188, 1-23, August 7, 2025. doi: 10

Insert

VARNAM1

Use

AAV

Promoter

Ef1a

Available Since

July 10, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-DIO-Varnam2-WPRE

Plasmid

#240055

Purpose

Cre dependent Red fluorescent, negative response-polarity voltage indicator for mammalian expression

Depositor

Mark Schnitzer

Article

Haziza et al Cell, 188, 1-23, August 7, 2025. doi: 10

Insert

VARNAM2

Use

AAV

Promoter

Ef1a

Available Since

July 10, 2025

Availability

Academic Institutions and Nonprofits only

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pC130: pAAV.CMV-CasRx-VEGFA sgRNA

Plasmid

#203443

Purpose

Plasmid expressing active RfxCas13d with VEGFA mRNA targeting gRNA

Depositor

Guei-Sheung Liu

Article

Kumar et al Proc Natl Acad Sci U S A. 2024 Nov 5;121

Inserts

U6-VEGFA sgRNA
RfxCas13d

Use

AAV

Tags

HA and NLS

Expression

Mammalian

Promoter

CMV and U6

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pM149: pAAV.CMV-Cas13e-VEGFA sgRNA

Plasmid

#203447

Purpose

Plasmid expressing active Cas13e with VEGFA mRNA targeting gRNA

Depositor

Guei-Sheung Liu

Article

Kumar et al Proc Natl Acad Sci U S A. 2024 Nov 5;121

Inserts

U6-VEGFA sgRNA
Cas13e

Use

AAV

Tags

HA and NLS

Expression

Mammalian

Promoter

CMV and U6

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pC368: pAAV.CMV-Cas13e-VEGFA sgRNA2

Plasmid

#227800

Purpose

Plasmid expressing active Cas13e with VEGFA mRNA targeting gRNA2

Depositor

Guei-Sheung Liu

Article

Kumar et al Proc Natl Acad Sci U S A. 2024 Nov 5;121

Inserts

U6-VEGFA sgRNA 2 (Vegfa Synthetic)
Cas13e

Use

AAV

Tags

HA and NLS

Expression

Mammalian

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pC369: pAAV.CMV-Cas13e-VEGFA sgRNA3

Plasmid

#227801

Purpose

Plasmid expressing active Cas13e with VEGFA mRNA targeting gRNA3

Depositor

Guei-Sheung Liu

Article

Kumar et al Proc Natl Acad Sci U S A. 2024 Nov 5;121

Inserts

U6-VEGFA sgRNA 3 (Vegfa Synthetic)
Cas13e

Use

AAV

Tags

HA and NLS

Expression

Mammalian

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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AAV-U6-sgLacZ-U6-sgGFP-hSyn1-mCherry

Plasmid

#208834

Purpose

Control plasmid expresses mCherry under the hSyn1 promoter with sgRNAs against LacZ and GFP

Depositor

Ben Emery

Article

Duncan et al Nat Commun. 2024 Oct 23;15(1):9148. doi:

Insert

mCherry

Use

AAV and CRISPR

Expression

Bacterial and Mammalian

Promoter

hSyn1

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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AAV-U6-sgMap3K12(1)-U6-sgMap3K12(2)-hSyn1-mCherry

Plasmid

#208835

Purpose

Knockout of Map3K12 (DLK) using tandem sgRNAs, expresses mCherry under the hSyn1 promoter

Depositor

Ben Emery

Article

Duncan et al Nat Commun. 2024 Oct 23;15(1):9148. doi:

Insert

mCherry

Use

AAV and CRISPR

Expression

Bacterial and Mammalian

Promoter

hSyn1

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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AAV-U6-sgMap3K13(1)-U6-sgMap3K13(2)-hSyn1-mCherry

Plasmid

#208836

Purpose

Knockout of Map3K13 (LZK) using tandem sgRNAs, expresses mCherry under the hSyn1 promoter

Depositor

Ben Emery

Article

Duncan et al Nat Commun. 2024 Oct 23;15(1):9148. doi:

Insert

mCherry

Use

AAV and CRISPR

Expression

Bacterial and Mammalian

Promoter

hSyn1

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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AAV-U6-sgMap3K13(1)-U6-sgMap3K12(1)-hSyn1-mCherry

Plasmid

#208837

Purpose

Knockout of Map3K12 (DLK) and Map3K13 (LZK) using tandem sgRNAs, expresses mCherry under the hSyn1 promoter

Depositor

Ben Emery

Article

Duncan et al Nat Commun. 2024 Oct 23;15(1):9148. doi:

Insert

mCherry

Use

AAV and CRISPR

Expression

Bacterial and Mammalian

Promoter

hSyn1

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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