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pDONR221-TAF15 No Stop

Plasmid

#84896

Purpose

DONOR vector for Gateway cloning of TAF15 No Stop

Depositor

Aaron Gitler

Article

Couthouis et al Proc Natl Acad Sci U S A. 2011 Dec 27;10

Insert

TAF15 (TAF15 Human)

Use

Gateway donor

Mutation

No Stop

Promoter

none

Available Since

Dec. 20, 2016

Availability

Academic Institutions and Nonprofits only

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pDONR223_AKT3_WT

Plasmid

#82093

Purpose

Gateway Donor vector containing AKT3 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

AKT3 (AKT3 Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_IDH2_p.R172M

Plasmid

#81618

Purpose

Gateway Donor vector containing IDH2 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

IDH2 (IDH2 Human)

Use

Gateway entry vector

Mutation

R172M

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pCRISPaint-mNeon-F-hgp100-PuroR [M1G]

Plasmid

#171801

Purpose

Universal donor plasmid for CRISPitope method encoding mNeon fluorescent protein, FLAG tag, human gp100 CD8+ T cell epitope [AA: 25-33 (KVPRNQDWL)] and puromycin resistance cassette [p.M1G]

Depositor

Michael Hoelzel

Article

Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.

Insert

mNeonGreen-FLAG-hgp100-T2A-PuroR

Use

Gene tagging

Mutation

PuroR: Changed Methionin 1 to Glycine.

Available Since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR223_AKT1_p.Q79K

Plasmid

#81479

Purpose

Gateway Donor vector containing AKT1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

AKT1 (AKT1 Human)

Use

Gateway entry vector

Mutation

Q79K

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_KRAS_p.D33E

Plasmid

#81659

Purpose

Gateway Donor vector containing KRAS,part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

KRAS (KRAS Human)

Use

Gateway entry vector

Mutation

D33E

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_IKBKG_WT

Plasmid

#82242

Purpose

Gateway Donor vector containing IKBKG , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

IKBKG (IKBKG Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_MAPK6_p.D387N

Plasmid

#81712

Purpose

Gateway Donor vector containing MAPK6, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

MAPK6 (MAPK6 Human)

Use

Gateway entry vector

Mutation

L290V; D387N; del amino acids 1-8

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_HRAS_WT

Plasmid

#81938

Purpose

Gateway Donor vector containing HRAS, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

HRAS (HRAS Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pLBCX-Large-T-antigenK1 mutant

Plasmid

#189978

Purpose

Retroviral vector used to immortalize human cells

Depositor

William Kaelin

Article

Shi et al Cancer Cell. 2022 Sep 12;40(9):939-956.e

Insert

Large-T-antigenK1 mutant

Available Since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR223_RASA1_p.L146S

Plasmid

#81606

Purpose

Gateway Donor vector containing RASA1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

RASA1 (RASA1 Human)

Use

Gateway entry vector

Mutation

L146S

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_RBPJ_WT

Plasmid

#82158

Purpose

Gateway Donor vector containing RBPJ , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

RBPJ (RBPJ Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_HRAS_p.Q61L

Plasmid

#81302

Purpose

Gateway Donor vector containing HRAS , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

HRAS (HRAS Human)

Use

Gateway entry vector

Mutation

Q61L

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_ERBB2_p.V842I

Plasmid

#81722

Purpose

Gateway Donor vector containing ERBB2, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

ERBB2 (ERBB2 Human)

Use

Gateway entry vector

Mutation

I655V; V842I

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_FBXW7_p.R658Q

Plasmid

#81502

Purpose

Gateway Donor vector containing FBXW7 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

FBXW7 (FBXW7 Human)

Use

Gateway entry vector

Mutation

R658Q

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_IDH1_p.R132L

Plasmid

#81679

Purpose

Gateway Donor vector containing IDH1, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

IDH1 (IDH1 Human)

Use

Gateway entry vector

Mutation

R132L

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_MAP2K1_p.E203K

Plasmid

#81729

Purpose

Gateway Donor vector containing MAP2K1, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

MAP2K1 (MAP2K1 Human)

Use

Gateway entry vector

Mutation

L197P; E203K; I310N

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_AKT1_p.D44N

Plasmid

#81456

Purpose

Gateway Donor vector containing AKT1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

NM_005163.2 (AKT1 Human)

Use

Gateway entry vector

Mutation

D44N

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_FBXW7_p.R465H

Plasmid

#81571

Purpose

Gateway Donor vector containing FBXW7 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

FBXW7 (FBXW7 Human)

Use

Gateway entry vector

Mutation

R465H

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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BAP1-C91A

Plasmid

#108440

Purpose

Stable expression of BAP1-C91A in mammalian cells.

Depositor

Emanuela Felley-Bosco, Rolf Stahel

Article

Okonska et al Mol Cancer Ther. 2019 Oct 16. pii: 1535-

Insert

BAP1 (BAP1 Human)

Expression

Mammalian

Mutation

C91A: c.271T>G, c.272G>C

Promoter

CMV

Available Since

Nov. 22, 2019

Availability

Academic Institutions and Nonprofits only

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