Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

Filter By

We narrowed to 6,946 results for: crispr cas9 plasmids

Showing: 5781 - 5800 of 6946 results

  1. pORFE0002

    Plasmid
    #112071
    Purpose
    AtCAS9 nickase D10A module CDS1
    Depositor
    Christian Chevalier
    Insert
    AtCAS9 nickase D10A
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Plant codon optimized nCas9 D10A S. pyogenes
    Promoter
    Available since
    Aug. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    112071-plasmid-map-sequence-id-217629

    Enlarge

  2. pORFE0003

    Plasmid
    #112072
    Purpose
    AtDeadCAS9 D10A H840A module CDS1
    Depositor
    Christian Chevalier
    Insert
    AtDeadCAS9 D10A H840A
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Plant codon optimized dCas9 D10A S. pyogenes
    Promoter
    Available since
    Aug. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    112072-plasmid-map-sequence-id-217628

    Enlarge

  3. pCMV-T7-PEmax-P2A-EGFP (LM1589)

    Plasmid
    #223136
    Purpose
    pCMV and pT7 Human expression plasmid for PEmax(nSpCas9(H840A)-M-MMLV_RT**)-P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Insert
    human codon optimized PEmax-P2A-EGFP
    Use
    CRISPR
    Tags
    BPNLS and NLS(SV40)-NLS(cMyc)-P2A-EGFP
    Expression
    Mammalian
    Mutation
    M-MLV mutations from PE2 (Anzalone et al. Nature …
    Promoter
    CMV and T7
    Available since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    223136-plasmid-map-sequence-id-447644

    Enlarge

  4. pLex307-UCOE-SFFV-puroR-MCP-HSF1-VIRF2

    Plasmid
    #218557
    Purpose
    Encodes SFFV promoter-driven MCP-HSF1-VIRF2 with an N-terminal bipartite SV40 NLS, along with puromycin resistance
    Depositor
    Alejandro Chavez
    Insert
    MCP-HSF1-VIRF2
    Use
    Lentiviral
    Tags
    SV40NLS
    Expression
    Mammalian
    Mutation
    Cas9 mutations to make dCas9=D10A+D839A+H840A+N86…
    Promoter
    Available since
    Aug. 13, 2025
    Availability
    Academic Institutions and Nonprofits only
    218557-plasmid-map-sequence-id-430698

    Enlarge

  5. PB_gRNA_S.pyogenes_scaffold_BB

    Plasmid
    #226429
    Purpose
    PiggyBac transposon vector backbone for cloning of sgRNA compatible with S.pyogenes dCas9 enzyme. BbsI Golden Gate site upstream of the scaffold for protospacer insertion. Puromycin selection marker.
    Depositor
    Alon Goren
    Type
    Empty backbone
    Use
    CRISPR; Piggybac transposon
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    Sept. 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    226429-plasmid-map-sequence-id-458099

    Enlarge

  6. loxP-G418-LoxP-TurboID-Rab11 HR

    Plasmid
    #230026
    Purpose
    Homology repair plasmid for endogenous tagging of Rab11 at the N-terminus with TurboID and a V5 epitope tag. Contains a G418 resistance cassette for selection of edited cells
    Depositor
    Francesca Bottanelli
    Article
    Adarska et al STAR Protoc. 2025 Aug 4;6(3):103949. doi
    Insert
    genomic sequence Rab11 locus (RAB11A Human)
    Use
    CRISPR
    Tags
    Turbo-ID, V5
    Expression
    Mutation
    Promoter
    Available since
    Jan. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    230026-plasmid-map-sequence-id-457126

    Enlarge

Showing: 5781 - 5800 of 6946 results