We narrowed to 28,378 results for: Tat

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pT5T_Rev7_1-211_SA112_KVK_YAK

Plasmid

#105630

Purpose

Expresses mouse monomeric Rev7 protein with mutations abolishing binding to Champ1 and Rev3

Depositor

Thanos Halazonetis

Insert

Rev7 (Mad2l2 Mouse)

Expression

Bacterial

Mutation

Mutations: F11S, G12A, I41Y, V132K, C133V, A135K,…

Promoter

T7

Available Since

March 14, 2022

Availability

Academic Institutions and Nonprofits only

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pJH3137

Plasmid

#179617

Purpose

Punc-4::GCaMP6::RFP unc-54 3' UTR C.elegans A-MNs and others expression of GCaMP6::RFP

Depositor

Mei Zhen

Article

Gao et al Elife. 2018 Jan 23;7. pii: 29915. doi: 1

Insert

GCaMP6

Tags

RFP

Expression

Worm

Promoter

Punc-4

Available Since

March 2, 2022

Availability

Academic Institutions and Nonprofits only

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pMVS184

Plasmid

#176294

Purpose

Reporter_ZIFUAS_minCMVpromoter_GFP_AAVS1_Puromycin

Depositor

Max Staller

Article

Staller et al Cell Syst. 2022 Apr 20;13(4):334-345.e5.

Insert

Promoter and GFP

Tags

GFP

Expression

Mammalian

Promoter

4x Zinc finger binding sites and mini CMV promoter

Available Since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pUC19-TgKI-MCS-p2A-QF2-MCS-polyA

Plasmid

#174026

Purpose

This plasmid is used for generating C-terminal knock-in plasmid and/or PCR donors in zebrafish.

Depositor

Michel Bagnat

Article

Levic et al Development. 2021 Sep 8. pii: 272090. do

Insert

p2A-QF2

Use

Zebrafish knock-in tagging

Available Since

Nov. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pKP925

Plasmid

#167591

Purpose

Express TrpC-TonB fusion protein

Depositor

Kathleen Postle

Article

Skare et al J Bacteriol. 1989 Aug;171(8):4442-7. doi

Insert

trpC-tonB

Expression

Bacterial

Mutation

Residues 1-25 TrpC; Residues 26-31 Linker; Residu…

Available Since

Oct. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pJH721_pJH_LldR_PlldR-sfGFP.20TAG

Plasmid

#163729

Purpose

Lactate-inducible expression of green fluorescent protein encoding twenty amber codons

Depositor

Matthew Bennett

Article

Ho et al Nucleic Acids Res. 2021 Mar 18;49(5):e25

Insert

lldr gfp.20TAG

Expression

Bacterial

Mutation

gfp with all 20 leucine codons mutated to amber

Available Since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pJH727_pJH_LldR_PlldR-sfGFP.10TAG

Plasmid

#163731

Purpose

Lactate-inducible expression of green fluorescent protein encoding ten amber codons

Depositor

Matthew Bennett

Article

Ho et al Nucleic Acids Res. 2021 Mar 18;49(5):e25

Insert

lldr gfp.10TAG

Expression

Bacterial

Mutation

gfp with first 10 leucine codons mutated to amber

Available Since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pJH774_pJH_LldR_PlldR-sfGFP.1opal

Plasmid

#163733

Purpose

Lactate-inducible expression of green fluorescent protein encoding one opal codon

Depositor

Matthew Bennett

Article

Ho et al Nucleic Acids Res. 2021 Mar 18;49(5):e25

Insert

lldr gfp.1TGA

Expression

Bacterial

Mutation

gfp with first leucine codon mutated to amber

Available Since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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Ssh2 gRNA#2

Plasmid

#163398

Purpose

Cas9-mediated knockout of Ssh2 in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Ssh2 (Ssh2 Mouse)

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pET-11a HIV-1 P6

Plasmid

#154201

Purpose

Bacterial expression of Gag P6 from HIV-1 HXB2 strain

Depositor

Lalit Deshmukh

Article

Ramaraju et al Biochemistry. 2021 Aug 3. doi: 10.1021/a

Insert

Gag P6 protein from HIV-1 clone HXB2

Tags

GB1-6xHis Tag

Expression

Bacterial

Promoter

T7

Available Since

July 30, 2021

Availability

Academic Institutions and Nonprofits only

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SPELL-ITSN1(DH-PH) Component 1

Plasmid

#170491

Purpose

SPELL ITSN1(DH-PH) component 1. mCherry-ITSN(N-lobe) fused to iFKBP. Split at loop 4 of DH domain.

Depositor

Klaus Hahn

Article

Dagliyan et al Nat Commun. 2018 Oct 2;9(1):4042. doi: 1

Insert

mCherry, ITSN(N-lobe), iFKBP

Expression

Mammalian

Available Since

July 27, 2021

Availability

Academic Institutions and Nonprofits only

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SPELL-ITSN1(DH-PH) Component 2

Plasmid

#170492

Purpose

SPELL ITSN1(DH-PH) component 2. myc-FRB-ITSN(C-lobe). Split at loop 4 of DH domain.

Depositor

Klaus Hahn

Article

Dagliyan et al Nat Commun. 2018 Oct 2;9(1):4042. doi: 1

Insert

myc, ITSN1(C-lobe), FRB

Expression

Mammalian

Available Since

July 27, 2021

Availability

Academic Institutions and Nonprofits only

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Dcp2 ΔH1 WD

Plasmid

#163580

Purpose

express Dcp2 ΔH1 WD at endogenous level in S.cerevisiae

Depositor

Michael Rosen

Article

Xing et al Elife. 2020 Jun 19;9. pii: 56525. doi: 1

Insert

Dcp2 (DCP2 Budding Yeast)

Tags

GFP

Expression

Yeast

Mutation

W50A, D54A, L255A

Promoter

DCP2 promoter

Available Since

June 7, 2021

Availability

Academic Institutions and Nonprofits only

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mCh-LaGFP(N)-cpFRB1-T2A-FKBP-LaGFP(C)

Plasmid

#165029

Purpose

Express cpFRB1-based GFP specific Chessbody (chemically switchable split nanobody); visualized by mCherry

Depositor

Yubin Zhou

Article

Lee et al J Mol Biol. 2020 May 1;432(10):3127-3136

Insert

LaGFP(N)-cpFRB1-T2A-FKBP-LaGFP(C)

Tags

mCherry

Expression

Bacterial and Mammalian

Promoter

T7

Available Since

May 26, 2021

Availability

Academic Institutions and Nonprofits only

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GFP-LaM(N)-cpFRB1-T2A-FKBP-LaM(C)

Plasmid

#165026

Purpose

Express cpFRB1-based mCherry specific Chessbody (chemically switchable split nanobody); visualized by GFP

Depositor

Yubin Zhou

Article

Lee et al J Mol Biol. 2020 May 1;432(10):3127-3136

Insert

LaM8(N)-cpFRB1-T2A-FKBP-LaM8(C)

Tags

GFP

Expression

Bacterial and Mammalian

Promoter

T7

Available Since

May 25, 2021

Availability

Academic Institutions and Nonprofits only

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GFP-LaM(N)-cpFRB2-T2A-FKBP-LaM(C)

Plasmid

#165028

Purpose

Express cpFRB2-based mCherry specific Chessbody (chemically switchable split nanobody); visualized by GFP

Depositor

Yubin Zhou

Article

Lee et al J Mol Biol. 2020 May 1;432(10):3127-3136

Insert

LaM8(N)-cpFRB2-T2A-FKBP-LaM8(C)

Tags

GFP

Expression

Bacterial and Mammalian

Promoter

T7

Available Since

May 17, 2021

Availability

Academic Institutions and Nonprofits only

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Vimentin-DiB-RM

Plasmid

#168783

Purpose

Vimentin - DiB-RM fusion protein

Depositor

Jens Meiler

Article

Bozhanova et al PLoS Comput Biol. 2021 Nov 8;17(11):e100

Insert

Vimentin-DiB-RM

Expression

Mammalian

Promoter

CMV

Available Since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

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Lyn-CFP-FRB1C_no-linker

Plasmid

#162434

Purpose

for use in CIT (chemically induced trimerization) system

Depositor

Takanari Inoue

Article

Wu et al Nat Methods. 2020 Sep;17(9):928-936. doi

Insert

Lyn-CFP-FRB1C_no_linker

Expression

Mammalian

Promoter

CMV

Available Since

May 4, 2021

Availability

Academic Institutions and Nonprofits only

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Dcp2 FL-H1

Plasmid

#163583

Purpose

express Dcp2 FL-H1 at endogenous level in S.cerevisiae

Depositor

Michael Rosen

Article

Xing et al Elife. 2020 Jun 19;9. pii: 56525. doi: 1

Insert

Dcp2 (DCP2 Budding Yeast)

Tags

GFP

Expression

Yeast

Mutation

The first HLM (EDQLKSYAEEQLKLLLGITKEE) is added t…

Promoter

DCP2 promoter

Available Since

April 16, 2021

Availability

Academic Institutions and Nonprofits only

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Dcp2 ΔN

Plasmid

#163575

Purpose

express wild type Dcp2 ΔN(243-970) at endogenous level in S.cerevisiae

Depositor

Roy Parker

Article

Xing et al Elife. 2020 Jun 19;9. pii: 56525. doi: 1

Insert

Dcp2 (DCP2 Budding Yeast)

Tags

GFP

Expression

Yeast

Mutation

Truncation of N-terminal domain (1-242) of Dcp2

Promoter

DCP2 promoter

Available Since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

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