We narrowed to 11,789 results for: nsf

1. AAV-hACE2-cMYC-Flag

   Plasmid

   #158957

   Purpose

   Used for expression of human ACE2 with AAV packaging

   Depositor

   Akiko Iwasaki

   Insert

   Human ACE2 (ACE2 Human)
   

   Use

   AAV

   Tags

   Flag and MYC

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Sept. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. RP4-7

   Plasmid

   #79811

   Purpose

   Modified broad host range plasmid RP4. Chloramphenicol resistant. Helper plasmid

   Depositor

   Michael Hynes

   Article

   Quandt et al Plasmid. 2004 Jul;52(1):1-12.

   Insert

   Chloramphenicol acetyltransferase
   

   Use

   Helper plasmid for mobilization

   Promoter

   Own promoter

   Available Since

   Aug. 19, 2016

   Availability

   Academic Institutions and Nonprofits only

3. pAAV-Camk2a(0.4)-DIO-Opto-cytTrkB(E281A)-HA

   Plasmid

   #180588

   Purpose

   Opto-cytTrkB

   Depositor

   Won Do Heo

   Article

   Hong J et al J Mol Biol. 2020 Feb 14;432(4):815-827.

   Insert

   TrkB
   

   Use

   AAV

   Mutation

   PHR(E281A)

   Available Since

   Feb. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. paavCAG-pre-mGRASP-2A-dTomato

   Plasmid

   #51902

   Purpose

   pre-2Ato

   Depositor

   Jinhyun Kim

   Article

   Kim et al Nat Methods. 2011 Dec 4;9(1):96-102. doi

   Insert

   presynaptic mGRASP
   

   Use

   AAV and Cre/Lox

   Available Since

   Jan. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV2-GCaMP6f-P2A-PAmCherry

   Plasmid

   #133412

   Purpose

   Fluorescent reporter for calcium imaging and photoactivation.

   Depositor

   Tim Holy

   Article

   Lee et al Science. 2019 Dec 13;366(6471):1384-1389

   Insert

   GCaMP6f-P2A-PAmCherry
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Dec. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. lentiGuide-Puro PRMT5-CRISPRi

   Plasmid

   #164637

   Purpose

   lentiGuide-Puro backbone with cloned target sequence for human PRMT5. The insert is: 5´- CACCGAGCCGCGTGTCCAGCGGGA-3. sgRNA achieves a downregulation of PRMT5 in combination with dCAS9-KRAB-MCP2

   Depositor

   Günter Schneider

   Insert

   target guide sequence PRMT5 inhibition (PRMT5 Human)
   

   Use

   CRISPR and Lentiviral; Interference

   Expression

   Mammalian

   Promoter

   U6 and EF1A

   Available Since

   May 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHA1

   Plasmid

   #85709

   Purpose

   This plasmid provides a BAC vector backbone, which can be used for cloning of BAC inserts, for instance, herpesvirus genomes

   Depositor

   Ulrich Koszinowski, Martin Messerle

   Article

   Adler et al J Virol. 2000 Aug;74(15):6964-74.

   Type

   Empty backbone

   Use

   Cre/Lox; Herpesvirus cloning

   Expression

   Bacterial

   Available Since

   June 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-beta-arrestin2-HA-TEV219

   Plasmid

   #104845

   Purpose

   b2AR-SPARK protease component

   Depositor

   Alice Ting

   Article

   Kim et al Elife. 2017 Nov 30;6. doi: 10.7554/eLife

   Insert

   beta-arrestin2-HA-TEV219
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pscAAV-CBh-aSyn-WPRE3-enSV40pA

   Plasmid

   #194244

   Purpose

   Expresses human alpha-Synuclein WT

   Depositor

   Michael J Fox Foundation MJFF

   Insert

   aSynuclein(WT) (SNCA Human)
   

   Use

   AAV

   Promoter

   CBh

   Available Since

   Jan. 17, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pMSCV-Flag-Mm Ezh2 Y641F

    Plasmid

    #80077

    Purpose

    Retroviral expression of mouse EZH2 Y641F mutant, hygromycin selection

    Depositor

    Phillip Abbosh

    Inserts

    Flag-EZH2 Y641F (Ezh2 Mouse)
    Hygromycin phosphotransferase
    

    Use

    Retroviral

    Tags

    5' FLAG

    Expression

    Mammalian

    Mutation

    Y641F, R342R, N393N

    Available Since

    July 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV.GFA104.PI.eGFP.WPRE.bGH

    Plasmid

    #100896

    Purpose

    AAV expression of EGFP in astrocytes

    Depositor

    Philip Haydon

    Article

    Ortinski et al Nat Neurosci. 2010 May;13(5):584-91. doi

    Has Service

    AAV5

    Insert

    EGFP
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    GFA104 (GFAP promoter)

    Available Since

    Jan. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-UbC-eGFP-F

    Plasmid

    #71545

    Purpose

    Expresses a membrane bound eGFP, under the UbC promoter. This promoter expresses transgenes in neurons at higher levels than plasmids with the CMV or β-actin-CMV promoters.

    Depositor

    Pantelis Tsoulfas

    Insert

    eGFP-F
    

    Use

    AAV

    Tags

    contains 20- amino-acid farnesylation signal from…

    Expression

    Mammalian

    Promoter

    UbC

    Available Since

    Dec. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pFRETtv-2in1-CN

    Plasmid

    #105119

    Purpose

    FRET/FLIM analysis in plants (stable or transient transformation)

    Depositor

    Christopher Grefen

    Article

    Hecker et al Plant Physiol. 2015 Jul;168(3):776-87. d

    Type

    Empty backbone

    Tags

    mTRQ2 and mVenus

    Expression

    Plant

    Available Since

    Feb. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-AP.Baz1a.1-GFP-WPRE

    Plasmid

    #211490

    Purpose

    expresses GFP under artificial promoter Baz1a

    Depositor

    Georg Keller

    Article

    O'Toole et al Neuron. 2023 Sep 6:S0896-6273(23)00626-8

    Insert

    GFP
    

    Use

    AAV

    Expression

    Mammalian

    Available Since

    Dec. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pFRETgc-2in1-CC

    Plasmid

    #105115

    Purpose

    FRET/FLIM analysis in plants (stable or transient transformation)

    Depositor

    Christopher Grefen

    Article

    Hecker et al Plant Physiol. 2015 Jul;168(3):776-87. d

    Type

    Empty backbone

    Tags

    mEGFP/mCherry

    Expression

    Plant

    Available Since

    April 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pFRETtv-2in1-CC

    Plasmid

    #105116

    Purpose

    FRET/FLIM analysis in plants (stable or transient transformation)

    Depositor

    Christopher Grefen

    Article

    Hecker et al Plant Physiol. 2015 Jul;168(3):776-87. d

    Type

    Empty backbone

    Tags

    mTRQ2/mVenus

    Expression

    Plant

    Available Since

    Feb. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV2-GCaMP5g-P2A-PAmCherry

    Plasmid

    #133411

    Purpose

    Fluorescent reporter for calcium imaging and photoactivation.

    Depositor

    Tim Holy

    Article

    Lee et al Science. 2019 Dec 13;366(6471):1384-1389

    Insert

    GCaMP5g-P2A-PAmCherry
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Feb. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAC1821_pCR8-SMN2-DN-RG6-SA

    Plasmid

    #118655

    Purpose

    Transient transfection; Expressed DR-SMN2-DN-RG6-SA-DR CasRx polycistronic gRNA with 3 spacers targeting SMN2 intron and 1 spacer targeting RG6 splice acceptor; Gateway Donor

    Depositor

    Albert Cheng

    Article

    Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

    Insert

    SMN2-DN-RG6-SA
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Jan. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pFRETtv-2in1-NN

    Plasmid

    #105125

    Purpose

    FRET/FLIM analysis in plants (stable or transient transformation)

    Depositor

    Christopher Grefen

    Article

    Hecker et al Plant Physiol. 2015 Jul;168(3):776-87. d

    Type

    Empty backbone

    Tags

    mTRQ2/mVenus

    Expression

    Plant

    Available Since

    Feb. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSLICER

    Plasmid

    #197288

    Purpose

    Conjugative plasmid that facilitates seamless deletions in D. radiodurans following insertion of a seamless deletion cassette. Contains an origin of transfer

    Depositor

    Bogumil Karas

    Article

    Brumwell et al Biodes Res. 2023 Mar 15;5:0009. doi: 10.

    Insert

    I-SceI Endonuclease
    

    Use

    Synthetic Biology; Bacterial and yeast cloning

    Available Since

    May 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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