We narrowed to 59,355 results for: cloning

1. pLCS108

   Plasmid

   #209329

   Purpose

   Gateway cloning compatible vector for transient expression in protoplasts. Arabidopsis UBQ10 promoter for N-terminal mCherry fusion protein.

   Depositor

   Lucia Strader

   Article

   Allen et al BMC Res Notes. 2025 Apr 8;18(1):149. doi

   Type

   Empty backbone

   Expression

   Plant

   Available Since

   Jan. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pXTK-BB1 [Backbone_RK2-KanR_PartTypeBB]

   Plasmid

   #229387

   Purpose

   XanthoMoClo Golden Gate Backbone Plasmid, contains Backbone_RK2-KanR_PartTypeBB and an sfGFP dropout

   Depositor

   Michael Springer

   Article

   Soltysiak et al ACS Synth Biol. 2025 Apr 18;14(4):1173-1

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   April 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCD-Ugi22

   Plasmid

   #207004

   Purpose

   pGGC000 Greengate entry vector containing Utricularia gibba insulator 22 (Ugi22) sequence (ID2547)

   Depositor

   Wayne Parrott

   Article

   Laspisa et al Front Plant Sci. 2023 Nov 8;14:1279231.

   Insert

   Utricularia gibba insulator 22
   

   Use

   Golden gate compatible cloning vector

   Available Since

   Nov. 8, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pCD-Ugi21

   Plasmid

   #207005

   Purpose

   pGGC000 Greengate entry vector containing Utricularia gibba insulator 21 (Ugi21) sequence (ID9063)

   Depositor

   Wayne Parrott

   Article

   Laspisa et al Front Plant Sci. 2023 Nov 8;14:1279231.

   Insert

   Utricularia gibba insulator 21
   

   Use

   Golden gate compatible cloning vector

   Available Since

   Nov. 8, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pcDNA type III TGF beta receptor (R3-OFF)

   Plasmid

   #10913

   Depositor

   Bob Weinberg

   Article

   Wang et al Cell. 1991 Nov 15. 67(4):797-805.

   Insert

   R3-OFF (Tgfbr3 Rat)
   

   Expression

   Mammalian

   Available Since

   Nov. 11, 2005

   Availability

   Academic Institutions and Nonprofits only

6. pcDNA5/FRT/TO HSPA6

   Plasmid

   #19459

   Depositor

   Harm Kampinga

   Article

   Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

   Insert

   HSPA6 (HSPA6 Human)
   

   Expression

   Mammalian

   Available Since

   Oct. 7, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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7. p667-UBC-ADCK5-V5-miniTurbo_IDG-K

   Plasmid

   #189909

   Purpose

   Gateway destination clone of ADCK5 (human) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomics

   Depositor

   Ben Major

   Insert

   ADCK5 (ADCK5 Human)
   

   Use

   Lentiviral; Gateway destination

   Tags

   V5-miniTurbo

   Expression

   Mammalian

   Promoter

   Ubiquitin

   Available Since

   Dec. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. p667-UBC-COQ8A-V5-miniTurbo_IDG-K

   Plasmid

   #189885

   Purpose

   Gateway destination clone of COQ8A (human) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomics

   Depositor

   Ben Major

   Insert

   COQ8A (COQ8A Human)
   

   Use

   Lentiviral; Gateway destination

   Tags

   V5-miniTurbo

   Expression

   Mammalian

   Promoter

   Ubiquitin

   Available Since

   Oct. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pL0M-S-mNeonGreen-EC18153

   Plasmid

   #137075

   Purpose

   Golden Gate Level 0 S module for N-terminal fluorescent protein tagging

   Depositor

   Julian Hibberd

   Article

   Luginbuehl et al Plant Direct. 2020 Feb 11;4(2):e00188. d

   Insert

   mNeonGreen DNA synthesis product with 5' and 3' extensions for Golden Gate cloning
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   June 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. TB:4xHRE_RE:MiniP

    Plasmid

    #178313

    Purpose

    4 copies of the HRE DNA binding motif

    Depositor

    Koen Venken

    Article

    Sarrion-Perdigones et al Methods Mol Biol. 2022;2524:409-432. doi

    Insert

    4 copies of the heat shock DNA binding motif
    

    Use

    Luciferase and Synthetic Biology

    Expression

    Mammalian

    Available Since

    July 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pXPR_124

    Plasmid

    #133461

    Purpose

    EF1a promoter expresses SpyoCas9 and P2A provides EGFP

    Depositor

    John Doench

    Article

    DeWeirdt et al Nat Commun. 2020 Feb 6;11(1):752. doi: 1

    Insert

    SpyoCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    Oct. 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pYSD099

    Plasmid

    #212923

    Purpose

    A MoClo Yeast Toolkit LEU2, CEN/ARS empty vector (GFP dropout). Contains ConL1, part234 GFP dropout, ConR2, LEU2, CEN6/ARS4, AmpR-ColE1.

    Depositor

    Paul Young

    Article

    O'Riordan et al ACS Synth Biol. 2024 Mar 14. doi: 10.102

    Insert

    GFP
    

    Expression

    Bacterial

    Available Since

    Feb. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCCl-MCS_miniCMV_TagBFP

    Plasmid

    #134986

    Purpose

    The plasmid includes a TagBFP gene driven by a minimal CMV promoter. Cis-regulatory elements can be cloned into the single EcoRV site. This plasmid can be used for generating a lentiviral vector

    Depositor

    Claudio Mussolino

    Article

    Roman Azcona et al Nat Protoc. 2020 Oct;15(10):3410-3440. d

    Insert

    TagBFP
    

    Use

    Lentiviral

    Promoter

    minCMV

    Available Since

    July 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRL1383a

    Plasmid

    #70692

    Purpose

    RSF1010-based vector with extensive polylinker, and resistance to Strep and Spec. Shuttle vector suitable for transfer to, and replication in, E. coli and Anabaena (Nostoc) sp. strain PCC 7120.

    Depositor

    Peter Wolk

    Article

    Wolk et al Arch Microbiol. 2007 Dec;188(6):551-63.

    Type

    Empty backbone

    Use

    Bacterial shuttle vector

    Available Since

    Jan. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDONR223_BIRC6

    Plasmid

    #197974

    Purpose

    Entry clone for wt BIRC6

    Depositor

    Eric Fischer

    Article

    Hunkeler et al Science. 2023 Mar 17;379(6637):1105-1111

    Insert

    BIRC6 (BIRC6 Human)
    

    Use

    Other

    Mutation

    L1332V

    Available Since

    March 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDIS3

    Plasmid

    #92173

    Purpose

    Vector containing the two fragments comprising the 550 bp NEUT5L region with NAT1 marker. Integration by disruption of the region targeted for recombination.

    Depositor

    Judith Berman

    Article

    Gerami-Nejad et al Microbiology. 2013 Mar;159(Pt 3):565-79.

    Insert

    NAT1
    

    Use

    Genbank: u03440.1

    Expression

    Yeast

    Available Since

    Oct. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pWBC

    Plasmid

    #15239

    Depositor

    Ron Vale

    Article

    Niclas et al Neuron. 1994 May . 12(5):1059-72.

    Insert

    Human neuronal kinesin heavy chain (KIF5A Human)
    

    Expression

    Bacterial

    Available Since

    Oct. 12, 2007

    Availability

    Academic Institutions and Nonprofits only

18. pLARRYv2-mScarlet

    Plasmid

    #233210

    Purpose

    Cloning expressed barcodes at the EcoRV site for preparing lentiviral libaries for single-cell lineage tracing experiments, with mScarlet marker

    Depositor

    Alejo Rodriguez-Fraticelli

    Article

    Singh et al Cell Stem Cell. 2025 Feb 19:S1934-5909(2

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Feb. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLARRYv2-T-Sapphire

    Plasmid

    #233209

    Purpose

    Cloning expressed barcodes at the EcoRV site for preparing lentiviral libaries for single-cell lineage tracing experiments, with T-Sapphire marker

    Depositor

    Alejo Rodriguez-Fraticelli

    Article

    Singh et al Cell Stem Cell. 2025 Feb 19:S1934-5909(2

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Nsp2Cas9_AAV

    Plasmid

    #192143

    Purpose

    Expresses Nsp2Cas9, and cloning backbone for sgRNA

    Depositor

    Yongming Wang

    Article

    Wei et al Elife. 2022 Aug 12;11. pii: 77825. doi:

    Insert

    Nsp2Cas9
    

    Use

    AAV

    Expression

    Mammalian

    Available Since

    April 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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