We narrowed to 11,743 results for: pcdna

1. pcDNA3.1/Myc-His(-)-HSulf-2 deltaCC

   Plasmid

   #13006

   Depositor

   Steven Rosen

   Article

   Morimoto-Tomita et al J Biol Chem. 2002 Dec 20. 277(51):49175-

   Insert

   human sulfatase 2 deltaCC (SULF2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   changed Cysteine 88 to Alanine

   Promoter

   Available since

   Oct. 30, 2006

   Availability

   Academic Institutions and Nonprofits only

2. Cyan Nano-lantern(Ca2+)/pcDNA3

   Plasmid

   #71344

   Purpose

   Express Cyan Nano-lantern-based luminescent Ca2+ indicator in mammalian cell

   Depositor

   Takeharu Nagai

   Article

   Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

   Insert

   Cyan Nano-lantern(Ca2+)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 7, 2015

   Availability

   Academic Institutions and Nonprofits only

3. pcDNA3.Flag.HERC2-HECT-C4762A.6xHis

   Plasmid

   #39228

   Depositor

   Aziz Sancar

   Article

   Kang et al Nucleic Acids Res. 2011 Apr;39(8):3176-8

   Insert

   HERC2 HECT Domain (HERC2 Human)
   

   Use

   Tags

   6xHis and Flag

   Expression

   Mammalian

   Mutation

   C4762A

   Promoter

   T7

   Available since

   July 31, 2012

   Availability

   Academic Institutions and Nonprofits only

4. pcDNA5-FRT-TO-Nsp8-Nsp7-HF_GC3opt

   Plasmid

   #157702

   Purpose

   mammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp8-Nsp7 fusion protein under control of a tetracycline-inducible promoter

   Depositor

   David Tollervey

   Article

   Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

   Insert

   Nsp8-Nsp7-HF_GC3opt (ORF1ab SARS-CoV-2)
   

   Use

   Tags

   His8-Flag

   Expression

   Mammalian

   Mutation

   codon optimized; gene expression was optimized by…

   Promoter

   Available since

   Aug. 5, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

5. XE257 pCDNA3.1(zeo)-hDsh3-truncDEP

   Plasmid

   #16752

   Depositor

   Randall Moon

   Article

   Angers et al Nat Cell Biol. 2006 Apr . 8(4):348-57.

   Insert

   FLAG-hDsh3-trunc before DEP (DVL3 Human)
   

   Use

   Tags

   flag

   Expression

   Mammalian

   Mutation

   truncated before DEP

   Promoter

   Available since

   March 14, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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6. p4979 pcDNA4-E6AP III L502P

   Plasmid

   #32892

   Depositor

   Peter Howley

   Article

   Cooper et al J Biol Chem. 2004 Sep 24;279(39):41208-1

   Insert

   E6AP (UBE3A Human)
   

   Use

   Tags

   6xHis Express and T7-tag

   Expression

   Mammalian

   Mutation

   L502P

   Promoter

   CMV

   Available since

   May 13, 2013

   Availability

   Academic Institutions and Nonprofits only

7. p4978 pcDNA4-E6AP III E550L

   Plasmid

   #32891

   Depositor

   Peter Howley

   Article

   Cooper et al J Biol Chem. 2004 Sep 24;279(39):41208-1

   Insert

   E6AP (UBE3A Human)
   

   Use

   Tags

   6xHis Express and T7-tag

   Expression

   Mammalian

   Mutation

   E550L

   Promoter

   CMV

   Available since

   May 13, 2013

   Availability

   Academic Institutions and Nonprofits only

8. pcDNA3-mCherry-mCherry-FLARE-Cameleon

   Plasmid

   #123352

   Purpose

   Red-fluorescent homoFRET/anisotropy-based genetically encoded calcium indicator.

   Depositor

   Jin Zhang

   Article

   Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

   Insert

   mCherry-mCherry-FLARE-Cameleon
   

   Use

   Tags

   6xHIS, T7 tag (gene 10 leader), Xpress (TM) tag, …

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA5FRT/FLAG-Ino80 ΔN (267-1556)

   Plasmid

   #29440

   Depositor

   Joan Conaway, Ronald Conaway

   Article

   Chen et al J Biol Chem. 2011 Feb 8. ():.

   Insert

   INO80 (INO80 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   INO80 267-1556 (a.a.)

   Promoter

   Available since

   June 7, 2011

   Availability

   Academic Institutions and Nonprofits only

10. EGFP Rab11a S25N pcDNA3.1 Zeo (+)

    Plasmid

    #206080

    Purpose

    dominant-negative Rab11a mutant - S25N mutation causes inhibition of Rab11a-dependent recycling

    Depositor

    Annette Dolphin

    Article

    Tran-Van-Minh et al J Neurosci. 2010 Sep 22;30(38):12856-67.

    Insert

    RAB11A (RAB11A Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    S25N

    Promoter

    CMV

    Available since

    Oct. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCDNA4TO R99A CoV-2 nsp1 3xFLAG

    Plasmid

    #176061

    Purpose

    Expresses R99A CoV2 nsp1 in mammalian cells. This contains a C-terminal 3xFLAG fusion to nsp1.

    Depositor

    Britt Glaunsinger

    Article

    Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

    Insert

    CoV-2 nsp1 (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. Cer(1-158)-beta-5 in pcDNAI/Amp

    Plasmid

    #55708

    Purpose

    An amino-terminal fragment of mCerulean was fused to Gbeta5. When co-expressed with a carboxyl terminal CFP fragment fused to a Ggamma subunit, a fluorescent signal is produced.

    Depositor

    Catherine Berlot

    Article

    Yost et al Mol Pharmacol. 2007 Oct;72(4):812-25. Ep

    Insert

    mCer(1-158)-beta5 (GNB5 Aequorea victoria, Human)
    

    Use

    Tags

    Cer(1-158) was fused to the amino terminus of Gbe…

    Expression

    Mammalian

    Mutation

    GBeta-5 was amplified via PCR, which added an N-t…

    Promoter

    CMV

    Available since

    July 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA 3.1 (+) FLAG-Ago2 S387A

    Plasmid

    #60652

    Purpose

    Expresses CMV-FLAG-Ago2 (N-term tag) S387A mutant in mammalian cells

    Depositor

    Carl Novina

    Article

    Horman et al Mol Cell. 2013 May 9;50(3):356-67. doi:

    Insert

    Argonaute 2 (AGO2 Human)
    

    Use

    Tags

    CMV-FLAG-Ago2 (N-term tag)

    Expression

    Mutation

    S387A mutant

    Promoter

    CMV

    Available since

    Dec. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCDNA3.0_mitoLAMA-G97 IRES ShadowG-mScarlet

    Plasmid

    #130709

    Purpose

    Plasmid encoding outer mitochondrial localization of LAMA-G97 for GFP as a SNAP-Tag-fusion and ShadowG-mScarlet

    Depositor

    Kai Johnsson

    Article

    Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

    Insert

    NTOM20-SNAP-Tag-LAMA-G97 IRES ShadowG-mScarlet
    

    Use

    Tags

    NTOM20 and SNAP-Tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3-Flag-RNF168 delta MIU1

    Plasmid

    #133978

    Purpose

    mammalian expression vector of Flag tagged RNF168 where the first motif interacting with ubiquitin (MIU) has been deleted

    Depositor

    Daniel Durocher

    Article

    Stewart et al Cell. 2009 Feb 6;136(3):420-34. doi: 10.

    Insert

    RNF168 (RNF168 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    deletion of MIU1

    Promoter

    CMV

    Available since

    Nov. 4, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. pcDNA3-RIPK3[S199A][S227A]-FLAG

    Plasmid

    #199323

    Purpose

    double mutated phosphorylation site (kinase acitivity and MLKL interaction inhibited)

    Depositor

    Adrian Press

    Article

    Hoff et al Cell Death Dis. 2023 Apr 18;14(4):275. d

    Insert

    Receptor-interacting serine/threonine-protein kinase 3 (RIPK3 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    changed Serine 199 and 227 to Alanine

    Promoter

    Available since

    May 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. p4973 pcDNA4-E6AP III C21Y

    Plasmid

    #32890

    Depositor

    Peter Howley

    Article

    Cooper et al J Biol Chem. 2004 Sep 24;279(39):41208-1

    Insert

    E6AP (UBE3A Human)
    

    Use

    Tags

    6 His Express and T7-tag

    Expression

    Mammalian

    Mutation

    C21Y

    Promoter

    CMV

    Available since

    May 13, 2013

    Availability

    Academic Institutions and Nonprofits only

18. pcDNA3.1 6B CARD8 UPA-CARD-mCherry WT

    Plasmid

    #164011

    Purpose

    Mammalian expression vector encoding CARD8 UPA-CARD with a C-terminal mCherry tag for imaging

    Depositor

    Hao Wu

    Article

    Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

    Insert

    CARD8 (CARD8 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Nano-lantern(Ca2+)_600/pcDNA3

    Plasmid

    #51982

    Purpose

    mammalian expression of a Nano-lantern-based luminescent Ca2+ indicator with Kd=600nM

    Depositor

    Takeharu Nagai

    Article

    Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

    Insert

    Nano-lantern_CaM_E104Q-2G
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    S257G in Rluc8

    Promoter

    Available since

    April 15, 2014

    Availability

    Academic Institutions and Nonprofits only

20. pcDNA5-FRT/TO-mNeonGreen-BRD4-p300

    Plasmid

    #204697

    Purpose

    This plasmid allows for inducible expression of engineered BRD4-p300 fusion tagged with mNeonGreen, in mammalian cells.

    Depositor

    Michael Rosen

    Article

    Kosno et al Sci Rep. 2023 Jul 24;13(1):11907. doi: 1

    Insert

    BRD4-p300 (BRD4, EP300 Human)
    

    Use

    Tags

    mNeonGreen

    Expression

    Mammalian

    Mutation

    direct fusion of BRD4 to p300

    Promoter

    CMV

    Available since

    Aug. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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