We narrowed to 9,951 results for: Uty

1. pGL3Basic_ME.1/ApoEpromoter

   Plasmid

   #51435

   Purpose

   This plasmid is a luciferase-based reporter construct to measure the activity of the ApoE promoter fused to ME.1 enhancer

   Depositor

   Sohail Tavazoie

   Article

   Pencheva et al Cell. 2014 Feb 27;156(5):986-1001. doi:

   Insert

   ME.1+ApoE promoter -980/+(93 bp.) (APOE, ME1 Human)
   

   Use

   Luciferase

   Expression

   Mammalian

   Mutation

   ME.1 enhancer is fused upstream of ApoE promoter …

   Available Since

   Feb. 27, 2014

   Availability

   Academic Institutions and Nonprofits only

2. pKLV2-U6gRNA3(BbsI)-PGKpuro2ABFP

   Plasmid

   #67990

   Purpose

   CRISPR gRNA expression vector with the conventional scaffold and puro/BFP markers without WPRE

   Depositor

   Kosuke Yusa

   Article

   Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

   Insert

   U6gRNA cassette with the conventional scaffold, PGKpuro2ABFP cassette
   

   Use

   CRISPR and Lentiviral

   Available Since

   Sept. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAAV-CAG-FLEX-Archon1-KGC-EGFP-ER2

   Plasmid

   #108422

   Purpose

   AAV production plasmid encoding for Archon1 fluorescent voltage reporter, Cre-dependent expression

   Depositor

   Edward Boyden

   Article

   Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

   Has Service

   AAV5

   Insert

   Archon1-KGC-EGFP-ER2
   

   Use

   AAV and Cre/Lox

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   June 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pBK2045-AAV-2xSp1-2xNFkB-EFSNC-dSaCas9-KRAB-MECP2(CMV-gRNA1)

   Plasmid

   #223165

   Purpose

   2nd gen. vector for expression of KRAB and truncated MECP2 with dSaCas9 and gRNA1 targeting CMV

   Depositor

   Boris Kantor

   Article

   Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

   Insert

   KRAB-MECP2 (MECP2 Human)
   

   Use

   AAV and CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Encodes aa 204-310 (MECP2)

   Promoter

   EF1a

   Available Since

   Aug. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCRISPaint-mNeon-BlastR [M1G]

   Plasmid

   #171805

   Purpose

   Universal donor plasmid for CRISPitope method encoding mNeon fluorescent protein and blasticidin resistance cassette [p.M1G]

   Depositor

   Michael Hoelzel

   Article

   Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.

   Insert

   mNeonGreen-T2A-BlastR
   

   Use

   Gene tagging

   Mutation

   BlastR: Changed Methionin 1 to Glycine.

   Available Since

   July 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pmiRFP-alpha-Tubulin-19-C

   Plasmid

   #108413

   Purpose

   Alpha-tubulin fused to monomeric near-infrared fluorescent protein miRFP

   Depositor

   Edward Boyden

   Article

   Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

   Insert

   alpha-tubulin b (Tuba1b Mouse)
   

   Tags

   miRFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAAV-CAG-FLEx-iSeroSnFR

   Plasmid

   #128486

   Purpose

   Fluorescent reporter for serotonin (conditional + membrane localization tag)

   Depositor

   Lin Tian

   Article

   Unger et al Cell. 2020 Dec 12. pii: S0092-8674(20)31

   Has Service

   AAV9

   Insert

   iSeroSnFR
   

   Use

   AAV

   Tags

   Ig-kappa leader, Myc, PDGFR, and cpsfGFP

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Dec. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pLKO.1-TRC.mKO2_shB3GNT5.1

   Plasmid

   #110325

   Purpose

   TRCN0000034809 (Target GCCTATGTAATCTCCGGTGAT), silence human B3GNT5 gene and express monomeric Kusabira-Orange2

   Depositor

   Sandra Martha Gomes Dias

   Article

   Quintero et al BMC Cancer. 2017 Nov 7;17(1):727. doi: 1

   Insert

   B3GNT5 (B3GNT5 Human)
   

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Promoter

   RNA polymerase III promoter for human U6 snRNA fo…

   Available Since

   June 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. CMV ArcLightCo (Q239)-T2A-nls-mCherry

   Plasmid

   #85806

   Purpose

   Genetically encoded voltage sensor ArcLight- codon optimized

   Depositor

   Vincent Pieribone

   Article

   Platisa et al ACS Chem Neurosci. 2017 Jan 3. doi: 10.1

   Insert

   ArcLightCo-Q239
   

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Ci-VSP contains R217Q mutation; super ecliptic pH…

   Promoter

   CMV

   Available Since

   Jan. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pmiRFP-Vimentin-17-C

    Plasmid

    #108414

    Purpose

    Vimentin fused to monomeric near-infrared fluorescent protein miRFP

    Depositor

    Edward Boyden

    Article

    Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

    Insert

    Vimentin (VIM Human)
    

    Tags

    miRFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pSAD-5PSD95-EGFP-SynPhRFP

    Plasmid

    #217979

    Purpose

    G-Deleted Rabies genomic plasmid to express 5PSD95-EGFP and SynPhRFP

    Depositor

    Edward Callaway

    Article

    Patino et al Elife. 2023 Dec 14;12:e89297. doi: 10.75

    Inserts

    5PSD95-EGFP (Dlg4 Rat)
    SynPhRFP (Syp Mouse)
    

    Use

    G-deleted rabies

    Tags

    EGFP and RFP

    Promoter

    None

    Available Since

    April 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCMV-T7-NLS(SV40)-(Spyo_Cas9)-6xHis-NLS(SV40)

    Plasmid

    #185706

    Purpose

    CMV and T7 promoter expression plasmid for human codon usage anCas(Spyo_Cas9) with an N-terminal NLS and C-terminal NLS

    Depositor

    Raul Perez-Jimenez

    Article

    Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:

    Insert

    Cas9
    

    Tags

    6xHis-NLS and NLS

    Expression

    Mammalian

    Mutation

    optimized for human codon usage

    Promoter

    CMV and T7

    Available Since

    June 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pOPINO_H3

    Plasmid

    #171927

    Purpose

    Bacterial expression of nanobody VHH_H3, which binds to receptor binding domain of the spike protein of SARS-CoV-2

    Depositor

    Ray Owens

    Article

    Huo et al Nat Commun. 2021 Sep 22;12(1):5469. doi:

    Insert

    VHH_H3
    

    Tags

    His6

    Expression

    Bacterial and Mammalian

    Available Since

    Sept. 23, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

14. pLenti GW V5 Eco/Dam hum LaminA

    Plasmid

    #182673

    Purpose

    Lentiviral eukaryotic expression vector with E.Coli Dam methylation fused to human Lamin A. Used in DamID experiments to methylate DNA that interacts in the proximity of Lamin A.

    Depositor

    Bas van Steensel

    Article

    Meuleman et al Genome Res. 2013 Feb;23(2):270-80. doi:

    Insert

    Lamin A (LMNA Human)
    

    Use

    Lentiviral

    Tags

    E. Coli Dam and V5

    Expression

    Mammalian

    Promoter

    HSP

    Available Since

    March 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. JDW 743 (pDONR221-EYFP-hKRAS4B-WT)

    Plasmid

    #156402

    Purpose

    Gateway middle entry clone encoding EYFP-fused to human KRAS4B, includes a stop codon

    Depositor

    Joshua Wythe

    Article

    Fish et al Circ Res. 2020 Jun 17. doi: 10.1161/CIRC

    Insert

    human wild type KRAS4B-WT (KRAS Human)
    

    Use

    Gateway cloning

    Tags

    EYFP

    Available Since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAAV-CaMKII-Archon1-KGC-EGFP-ER2

    Plasmid

    #108419

    Purpose

    AAV production plasmid encoding for Archon1 fluorescent voltage reporter

    Depositor

    Edward Boyden

    Article

    Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

    Insert

    Archon1-KGC-EGFP-ER2
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    CaMKII

    Available Since

    April 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pBK2047-AAV-2xSp1-2xNFkB-EFSNC-dSaCas9-KRAB-MECP2(APOE-gRNA2)

    Plasmid

    #223167

    Purpose

    2nd gen. vector for expression of KRAB and truncated MECP2 with dSaCas9 and gRNA2 targeting human/mouse APOE

    Depositor

    Boris Kantor

    Article

    Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

    Insert

    KRAB-MECP2 (MECP2 Human)
    

    Use

    AAV and CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Encodes aa 204-310 (MECP2)

    Promoter

    EF1a

    Available Since

    Aug. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pSt1374m-EF1alpha-N-NLS-DNMT3L-L-DNMT3A-L-dcas9-NLS

    Plasmid

    #112214

    Purpose

    Targeted DNA methylation

    Depositor

    Xingxu Huang

    Article

    Lu et al Cell Death Dis. 2020 Feb 3;11(2):85. doi

    Insert

    DNMT3L-L-DNMT3A-L-dcas9 (DNMT3L, DNMT3A Human)
    

    Use

    CRISPR

    Tags

    mCherry

    Mutation

    D10A,H840A,N863A

    Available Since

    Aug. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. px335 Mettl14 sgRNA #2

    Plasmid

    #61514

    Purpose

    encodes sgRNA sequence for targeting mouse Mettl14 locus (Cas9-Nickase strategy)

    Depositor

    Jacob Hanna

    Article

    Geula et al Science. 2015 Feb 27;347(6225):1002-6. d

    Insert

    gccgctcccggatctcctgc
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Jan. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

20. pOPINO_C5

    Plasmid

    #171925

    Purpose

    Bacterial expression of nanobody VHH_C5, which binds to receptor binding domain of the spike protein of SARS-CoV-2

    Depositor

    Ray Owens

    Article

    Huo et al Nat Commun. 2021 Sep 22;12(1):5469. doi:

    Insert

    VHH_C5
    

    Tags

    His6

    Expression

    Bacterial

    Available Since

    Sept. 23, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge