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Showing: 5881 - 5900 of 7379 results

  5. MSP2283

    Plasmid
    #70702
    Purpose
    Bacterial expression plasmid for SaCas9 & sgRNA targeted to site 1: T7-humanSaCas9-NLS-3xFLAG-T7-Sa-sgRNA(84) #1
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038
    Inserts
    mammalian codon-optimized Staphylococcus aureus Cas9
    SaCas9 sgRNA (+84)
    Use
    CRISPR
    Tags
    NLS-3xFLAG
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Dec. 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    70702-plasmid-map-sequence-id-127769

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  8. prCAG-RBS"m1"-sfGFP

    Plasmid
    #191400
    Purpose
    Expression of 47 rCAG repeats fused to a translational unit over-expressing superfolder GFP using a strong RBS. The RBS contains mutation (m1)
    Depositor
    Ariel Lindner
    Article
    Guo et al Cell. 2022 Sep 29;185(20):3823-3837.e23.
    Insert
    47xCAG RBS"m1"-sfGFP
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PLtet0-1
    Available since
    June 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    191400-plasmid-map-sequence-id-382526

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  10. N-BLa 1.1

    Plasmid
    #88994
    Purpose
    BLaTM, a genetic tool to measure homo- and heterotypic transmembrane helix-helix interactions. Plasmid encoding the 1.1 fusionprotein containing the N-terminal fragment of the split β-lactamase.
    Depositor
    Dieter Langosch
    Article
    Schanzenbach et al Sci Rep. 2017 Mar 7;7:43476. doi: 10.103
    Insert
    N-BLa 1.1 fusion protein
    Use
    Tags
    Flag
    Expression
    Bacterial
    Mutation
    Promoter
    araBAD
    Available since
    May 2, 2017
    Availability
    Academic Institutions and Nonprofits only
    88994-plasmid-map-sequence-id-177496

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  11. pGBW-m4046810

    Plasmid
    #149251
    Purpose
    Bacterial expression plasmid for SARS-CoV-2 N (nucleocapsid)
    Depositor
    Benjie Chen, Ginkgo Bioworks
    Insert
    SARS-CoV-2 N (nucleocapsid) (N Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Cleavable TEV;HA
    Expression
    Bacterial
    Mutation
    Escherichia coli recode 1
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available since
    May 11, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    149251-plasmid-map-sequence-id-357431

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Showing: 5881 - 5900 of 7379 results