Addgene

We narrowed to 14,558 results for: pET

Showing: 2951 - 2960 of 14558 results

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pFA6a-link-ymYPET-URA3

Plasmid

#168056

Purpose

Replaces Addgene plasmid 118460. Yeast tagging vector to create a gene fusion with yeast optimized mYPET with CaUra3 selection; Contains the linker region at the 5’ end of the FP

Depositor

Bas Teusink

Article

Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

Insert

ymYPET

Use

Tags

ymYPET

Expression

Yeast

Mutation

Promoter

Available since

June 18, 2021

Availability

Academic Institutions and Nonprofits only

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[pSK104] pET28 SLC38A9N-His

Plasmid

#136142

Purpose

bacterial expression of SLC38A9 N term fragment (1-119)

Depositor

Kuang Shen, David Sabatini

Article

Shen et al Proc Natl Acad Sci U S A. 2018 Sep 18;11

Insert

SLC38A9 N term fragment (1-119) (SLC38A9 Human)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

April 15, 2020

Availability

Academic Institutions and Nonprofits only

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pET21b-Caspase-3(D3A,V266E)

Plasmid

#90091

Purpose

Expresses caspase D3A and V266E mutant in bacterial cells

Depositor

Clay Clark

Article

Pop et al Biochemistry. 2003 Oct 28;42(42):12311-2

Insert

Caspase 3 (CASP3 Human)

Use

Tags

His tag

Expression

Bacterial

Mutation

D175A D28A D9A V266E

Promoter

Available since

May 2, 2017

Availability

Academic Institutions and Nonprofits only

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pET-6xHis-(pos28)GFP

Plasmid

#89250

Purpose

Expresses GFP with +28 theoretical charge in E.Coli

Depositor

David Liu

Insert

His6-(pos16)-GFP

Use

Tags

His6

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 21, 2017

Availability

Academic Institutions and Nonprofits only

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pET-26b-NbVHH05-HA-His

Plasmid

#171567

Purpose

Bacterial expression of HA-His-tagged anti-UBC6e (human) recombinant alpaca nanobody (NbVHH05-HA-His)

Depositor

Norbert Perrimon, Hidde Ploegh

Article

Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

Insert

NbVHH05-HA-His (UBE2J1 Human)

Use

Tags

HA-tag/His-tag and PelB signal peptide

Expression

Bacterial

Mutation

Promoter

Available since

June 30, 2021

Availability

Academic Institutions and Nonprofits only

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pET24a HAB1 (172-511)

Plasmid

#177844

Purpose

Bacterial Expression of HAB1

Depositor

Karsten Melcher

Article

Melcher et al Nature. 2009 Dec 3;462(7273):602-8. doi:

Insert

Protein phosphatase 2C 16

Use

Tags

6xHis-GST

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pET28a-SARS-CoV-2 NSP13

Plasmid

#176492

Purpose

Expresses the N-terminal His6-tagged SARS-CoV2 helicase (NSP13) in E coli.

Depositor

Xiaodong Cheng

Article

White et al J Phys Chem Lett. 2020 Nov 5;11(21):9144

Insert

SARS-CoV-2 NSP13 (ORF1ab SARS-CoV)

Use

Tags

His6 and T7

Expression

Bacterial

Mutation

I570 from SARS-CoV NSP13 was mutated to V to conv…

Promoter

T7

Available since

Jan. 10, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pET22b-mEB1-eGFP-6xHis

Plasmid

#130981

Purpose

Bacterial expression of mouse EB1-GFP-6xHis for protein purification.

Depositor

Anne Straube

Article

Roth et al J Cell Sci. 2018 Oct 31;132(4). pii: jcs

Insert

EB1-GFP-6xHis (Mapre1 Mouse)

Use

Tags

eGFP-6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pET28-his6-HA3-SNAPf

Plasmid

#188941

Purpose

T7 expression vector for making his6- and HA3-tagged SNAP protein

Depositor

Stephen Buratowski

Article

Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

Insert

his6-HA3-SNAPf

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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PET-B-eSpCas9-NLS-6xHis

Plasmid

#207383

Purpose

Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

B-eSpCas9

Use

CRISPR

Tags

6xHis

Expression

Bacterial

Mutation

K848A, K1003A, R1060A, amino acids 1005-1013 repl…

Promoter

T7

Available since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

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