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pDG03246

Plasmid

#195997

Purpose

Nanobody Proj-Nano29 production

Depositor

Dirk Görlich

Article

Loreau et al eLife. 2023 Jan 16;12:e79343. doi: 10.75

Insert

H14-SUMO-Cys- ProjNano29-Cys

Use

E.coli expression

Available Since

Feb. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pDG03248

Plasmid

#195991

Purpose

Nanobody Sls-Nano2 production

Depositor

Dirk Görlich

Article

Loreau et al eLife. 2023 Jan 16;12:e79343. doi: 10.75

Insert

H14-SUMO-Cys- SlsNano2-Cys

Use

E.coli expression

Available Since

Feb. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pDG03775

Plasmid

#196003

Purpose

Nanobody Proj-Nano37 production

Depositor

Dirk Görlich

Article

Loreau et al eLife. 2023 Jan 16;12:e79343. doi: 10.75

Insert

H14-NEDD8- ProjNano37-Cys

Use

E.coli expression

Available Since

Feb. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pDG03773

Plasmid

#196001

Purpose

Nanobody Proj-Nano34 production

Depositor

Dirk Görlich

Article

Loreau et al eLife. 2023 Jan 16;12:e79343. doi: 10.75

Insert

H14-NEDD8- ProjNano34-Cys

Use

E.coli expression

Available Since

Feb. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pDG04109

Plasmid

#196011

Purpose

Nanobody Actn-Nano63 production

Depositor

Dirk Görlich

Article

Loreau et al eLife. 2023 Jan 16;12:e79343. doi: 10.75

Insert

H14-SUMO-Cys- ActnNano63-Cys

Use

E.coli expression

Available Since

Feb. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pDG03779

Plasmid

#196004

Purpose

Nanobody Proj-Nano46 production

Depositor

Dirk Görlich

Article

Loreau et al eLife. 2023 Jan 16;12:e79343. doi: 10.75

Insert

H14-NEDD8- ProjNano46-Cys

Use

E.coli expression

Available Since

Feb. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pDG03774

Plasmid

#196002

Purpose

Nanobody Proj-Nano35 production

Depositor

Dirk Görlich

Article

Loreau et al eLife. 2023 Jan 16;12:e79343. doi: 10.75

Insert

H14-NEDD8- ProjNano35-Cys

Use

E.coli expression

Available Since

Feb. 27, 2023

Availability

Academic Institutions and Nonprofits only

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UAS-GFP::NMN-Deamidase

Plasmid

#187868

Purpose

Gal4/UAS expression of GFP::NMN-Deamidase

Depositor

Lukas Neukomm

Article

Llobet Rosell et al Elife. 2022 Dec 7;11:e80245. doi: 10.755

Insert

NMN-Deamidase

Tags

GFP

Expression

Insect

Available Since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pCfB9341 (pgRNA_IX-1_NatMX)

Plasmid

#161593

Purpose

EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site IX-1

Depositor

Irina Borodina

Article

Babaei et al FEMS Yeast Res. 2021 May 11;21(4). pii:

Insert

guiding RNA

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available Since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pCfB9343 (pgRNA_XV-1_NatMX)

Plasmid

#161595

Purpose

EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site XV-1

Depositor

Irina Borodina

Article

Babaei et al FEMS Yeast Res. 2021 May 11;21(4). pii:

Insert

guiding RNA

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available Since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pCfB9358(VII-1_Markerfree_BackBone)

Plasmid

#161599

Purpose

EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site VII-1, (Chr VII: 438509..438490)

Depositor

Irina Borodina

Article

Babaei et al FEMS Yeast Res. 2021 May 11;21(4). pii:

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available Since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pCfB9359(VIII-1_Markerfree_BackBone)

Plasmid

#161600

Purpose

EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site VIII-1, (Chr VIII: 293615..293634)

Depositor

Irina Borodina

Article

Babaei et al FEMS Yeast Res. 2021 May 11;21(4). pii:

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available Since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pCfB9361(XIII-1_Markerfree_BackBone)

Plasmid

#161602

Purpose

EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XIII-1, (Chr XIII: 306804..306823)

Depositor

Irina Borodina

Article

Babaei et al FEMS Yeast Res. 2021 May 11;21(4). pii:

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available Since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pCfB9363(XVI-1_Markerfree_BackBone)

Plasmid

#161604

Purpose

EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XVI-1, (Chr XVI: 406267..406286)

Depositor

Irina Borodina

Article

Babaei et al FEMS Yeast Res. 2021 May 11;21(4). pii:

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available Since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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KBB345

Plasmid

#185078

Purpose

rfa3D46 truncation fused to GFP

Depositor

Ken Belanger

Article

Belanger et al DNA Cell Biol. 2011 Sep;30(9):641-51. do

Insert

RFA3

Tags

GFP

Expression

Yeast

Mutation

Rfa3 truncation aa 1-46

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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KBB349

Plasmid

#185080

Purpose

rfa2D248 truncation fused to GFP

Depositor

Ken Belanger

Article

Belanger et al DNA Cell Biol. 2011 Sep;30(9):641-51. do

Insert

RFA2

Tags

GFP

Expression

Yeast

Mutation

Rfa2 truncation aa 1-247

Available Since

July 21, 2022

Availability

Academic Institutions and Nonprofits only

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pEUK066

Plasmid

#180830

Purpose

T7-inducible expression construct to produce NaGdmA (SARO_RS07455) in E. coli

Depositor

Gregg Beckham

Article

Bleem et al Chem Catalysis, 2022

Insert

NaGdmA

Tags

6X His

Expression

Bacterial

Promoter

T7

Available Since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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PylT(AGGA)Ev2 PylS mCherry-P2A-eGFP(AGGA)

Plasmid

#174528

Purpose

Dual-fluorescence reporter for quantifying PylT(AGGA)Ev2 decoding efficiency

Depositor

Yu-Hsuan Tsai

Article

Mills et al Cell Rep Methods. 2021 Sep 16;1(6):10007

Insert

PylS

Tags

Flag

Expression

Mammalian

Available Since

Oct. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pAT15065-HF-CBE

Plasmid

#163010

Purpose

plasmid expressing HF-CBE

Depositor

Ervin Welker

Article

Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

Insert

HF-CBE

Use

CRISPR

Tags

3xFLAG and SV40 NLS

Expression

Mammalian

Available Since

Sept. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pAT15066-Hypa-CBE

Plasmid

#163011

Purpose

plasmid expressing Hypa-CBE

Depositor

Ervin Welker

Article

Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

Insert

Hypa-CBE

Use

CRISPR

Tags

3xFLAG and SV40 NLS

Expression

Mammalian

Available Since

Sept. 21, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge