We narrowed to 9,951 results for: Uty

1. pSt1374-N-NLS-DNMT3L-L-DNMT3A-L-dcas9-NLS

   Plasmid

   #112210

   Purpose

   Targeted DNA methylation

   Depositor

   Xingxu Huang

   Article

   Lu et al Cell Death Dis. 2020 Feb 3;11(2):85. doi

   Insert

   DNA methyltransferase 3 alpha (DNMT3A, DNMT3L Human)
   

   Use

   CRISPR

   Mutation

   D10A,H840A,N863A

   Available Since

   Oct. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSt1374-N-NLS-DNMT3L-L-DNMT3Amut-L-dcas9-NLS

   Plasmid

   #112209

   Purpose

   Targeted DNA methylation

   Depositor

   Xingxu Huang

   Article

   Lu et al Cell Death Dis. 2020 Feb 3;11(2):85. doi

   Insert

   DNMT3L-L-DNMT3Amut-L-dcas9 (DNMT3L, DNMT3A Human)
   

   Use

   CRISPR

   Mutation

   D10A,H840A,N863A,C710S

   Available Since

   Oct. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pOPINO_C1

   Plasmid

   #171924

   Purpose

   Bacterial expression of nanobody VHH_C1, which binds to receptor binding domain of the spike protein of SARS-CoV-2

   Depositor

   Ray Owens

   Article

   Huo et al Nat Commun. 2021 Sep 22;12(1):5469. doi:

   Insert

   VHH_C1
   

   Tags

   His6

   Expression

   Bacterial

   Available Since

   Sept. 23, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pKLV2-U6gRNA5(Empty)-PGKGFP2ABFP-W

   Plasmid

   #67983

   Purpose

   Cas9 activity reporter (control) with GFP and BFP

   Depositor

   Kosuke Yusa

   Article

   Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

   Insert

   U6gRNA cassette, PGKGFP2ABFP cassette, WPRE
   

   Use

   CRISPR and Lentiviral

   Mutation

   Deleted BbsI site within WPRE, modified BFP

   Available Since

   Sept. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. JDW 836 (pCS2-V5-mScarlet-I-hsKRAS4A-WT)

   Plasmid

   #156411

   Purpose

   pCS2 CMV expression vector driving V5-tagged mScarlet-I-fused human KRAS4A

   Depositor

   Joshua Wythe

   Article

   Fish et al Circ Res. 2020 Jun 17. doi: 10.1161/CIRC

   Insert

   human wild type KRAS4A-WT (KRAS Human)
   

   Tags

   mScarlet-I

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. PET.SUMO eIF4G1 1-200

   Plasmid

   #158791

   Purpose

   Bacterial expression vector for the expression of N-terminal 6xHIS-SUMO-tagged codon optimized eIF4G1 amino acids 1-200 isoform lacking the microexon.

   Depositor

   Benjamin Blencowe

   Article

   Gonatopoulos-Pournatzis et al Mol Cell. 2020 Mar 19;77(6):1176-1192.e1

   Insert

   eIF4G1 (codon optimized)
   

   Tags

   6xHIS and SUMO

   Expression

   Bacterial

   Mutation

   amino acids 1-200

   Promoter

   T7

   Available Since

   Aug. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-CAG-Archon2-KGC-EGFP-ER2

   Plasmid

   #108421

   Purpose

   AAV production plasmid encoding for Archon2 fluorescent voltage reporter

   Depositor

   Edward Boyden

   Article

   Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

   Insert

   Archon2-KGC-EGFP-ER2
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   June 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSt1374m-EF1alpha-N-NLS-DNMT3L-L-DNMT3Amut-L-dcas9-NLS

   Plasmid

   #112215

   Purpose

   Targeted DNA methylation

   Depositor

   Xingxu Huang

   Article

   Lu et al Cell Death Dis. 2020 Feb 3;11(2):85. doi

   Insert

   DNMT3L-L-DNMT3Amut-L-dcas9 (DNMT3A, DNMT3L Human)
   

   Use

   CRISPR

   Tags

   mCherry

   Mutation

   D10A,H840A,N863A,C710S

   Available Since

   Aug. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pOPINO_F2

   Plasmid

   #171926

   Purpose

   Bacterial expression of nanobody VHH_F2, which binds to receptor binding domain of the spike protein of SARS-CoV-2

   Depositor

   Ray Owens

   Article

   Huo et al Nat Commun. 2021 Sep 22;12(1):5469. doi:

   Insert

   VHH_F2
   

   Tags

   His6

   Expression

   Bacterial, Insect, and Mamm…

   Available Since

   Sept. 23, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. JDW 763 (pCS2-EYFP-hsKRAS4B-WT)

    Plasmid

    #156408

    Purpose

    pCS2 CMV expression vector driving EYFP fused human KRAS4B

    Depositor

    Joshua Wythe

    Article

    Fish et al Circ Res. 2020 Jun 17. doi: 10.1161/CIRC

    Insert

    human wild type KRAS4B-WT (KRAS Human)
    

    Tags

    EYFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTol2-UAS-zArchon2-KGC-EGFP-ER2

    Plasmid

    #108428

    Purpose

    Codon-optimized Archon2 fluorescent voltage reporter in zebrafish expression plasmid

    Depositor

    Edward Boyden

    Article

    Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

    Insert

    zArchon2-KGC-EGFP-ER2
    

    Use

    Zebrafish

    Promoter

    UAS

    Available Since

    May 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMXS-IRES-BLAST flag-CAD hCPS1 AD

    Plasmid

    #188130

    Purpose

    Expresses C-terminal flag-tagged human CAD hCPS1 AD in mammalian cells

    Depositor

    Richard Possemato

    Article

    Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

    Insert

    CAD (CAD Human)
    

    Use

    Retroviral

    Tags

    Flag tag

    Expression

    Mammalian

    Mutation

    TCC -> AGT silent mutations at nt439-441 elimi…

    Available Since

    Feb. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. hSyn ArcLightCo (Q239)-T2A-nls-mCherry

    Plasmid

    #85844

    Purpose

    Genetically encoded voltage sensor ArcLight- codon optimized

    Depositor

    Vincent Pieribone

    Article

    Platisa et al ACS Chem Neurosci. 2017 Jan 3. doi: 10.1

    Insert

    ArcLightCo-Q239
    

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Ci-VSP contains R217Q mutation; super ecliptic pH…

    Promoter

    hSyn1

    Available Since

    Jan. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLKO-PHB-8

    Plasmid

    #122233

    Purpose

    Expresses shRNA targeting the coding sequence of human PHB

    Depositor

    Alex Kentsis

    Article

    Ortiz et al JCI Insight. 2019 Aug 8;4(15). pii: 1270

    Insert

    shRNA targeting PHB (see partial depositor seq) (PHB1 Human)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Available Since

    Aug. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pInducer20_SLC38A2_wt

    Plasmid

    #156182

    Purpose

    Dox-inducible expression of sgRNA-resistant SLC38A2 wild-type cDNA.

    Depositor

    Alec Kimmelman

    Article

    Parker et al Cancer Discov. 2020 Apr 27. pii: 2159-82

    Insert

    SLC38A2 (SLC38A2 Human)
    

    Expression

    Mammalian

    Mutation

    Silent mutations to prevent targeting by sgRNA: T…

    Promoter

    TRE

    Available Since

    Aug. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLKO-PHB-4

    Plasmid

    #122231

    Purpose

    Expresses shRNA targeting the 3' UTR of human PHB

    Depositor

    Alex Kentsis

    Article

    Ortiz et al JCI Insight. 2019 Aug 8;4(15). pii: 1270

    Insert

    PHB (PHB1 Human)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Available Since

    Aug. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. PET.SUMO eIF4G1 +MIC 1-200

    Plasmid

    #158792

    Purpose

    Bacterial expression vector for the expression of N-terminal 6xHIS-SUMO-tagged codon optimized eIF4G1 amino acids 1-200 isoform including the microexon.

    Depositor

    Benjamin Blencowe

    Article

    Gonatopoulos-Pournatzis et al Mol Cell. 2020 Mar 19;77(6):1176-1192.e1

    Insert

    eIF4G1 (with microexon) codon optimized
    

    Tags

    6xHIS and SUMO

    Expression

    Bacterial

    Mutation

    amino acids 1-200

    Promoter

    T7

    Available Since

    Aug. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. px335 Mettl14 sgRNA #1

    Plasmid

    #61515

    Purpose

    encodes sgRNA sequence for targeting mouse Mettl14 locus (Cas9-Nickase strategy)

    Depositor

    Jacob Hanna

    Article

    Geula et al Science. 2015 Feb 27;347(6225):1002-6. d

    Insert

    gcggcagctcctagctcagc
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Jan. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

19. pMEXneo-TrkC-TrkB-hybrid

    Plasmid

    #190205

    Purpose

    To express a Human TrkC receptor that binds only BDNF. The second IgG like domain which is the main binding domain comes from the TrkB receptor

    Depositor

    Pantelis Tsoulfas

    Article

    Urfer et al J Biol Chem. 1998 Mar 6;273(10):5829-40.

    Insert

    TrkC (NTRK3 Human)
    

    Expression

    Mammalian

    Mutation

    The second IgG like domain and the extracellular …

    Promoter

    Moloney mouse sarcoma virus right LTR

    Available Since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pmiRFP-H2B-C1

    Plasmid

    #108411

    Purpose

    Histone 2B fused to monomeric near-infrared fluorescent protein miRFP

    Depositor

    Edward Boyden

    Article

    Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

    Insert

    miRFP-H2B (H2BC21 Human)
    

    Tags

    miRFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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