We narrowed to 7,513 results for: Ski;

1. pMS21

   Plasmid

   #229476

   Purpose

   Expresses RssB with E135A, E136A, & E137A mutation with His-tag

   Depositor

   Alexandra Deaconescu

   Article

   Dorich et al Genes Dev. 2019 Jun 1;33(11-12):718-732.

   Insert

   RssB (E135A, E136A, E137A)
   

   Use

   Tags

   10xHis

   Expression

   Bacterial

   Mutation

   E135A, E136A, E137A

   Promoter

   T7

   Available Since

   Jan. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pVF18

   Plasmid

   #229456

   Purpose

   Expresses IraD (aa17-130) with His-tag

   Depositor

   Alexandra Deaconescu

   Article

   Dorich et al Genes Dev. 2019 Jun 1;33(11-12):718-732.

   Insert

   IraD (aa 17-130 only)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   aa 17-130 only

   Promoter

   T7

   Available Since

   Jan. 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAM201

   Plasmid

   #227660

   Purpose

   pRS305 3XFLAG-HRV3C-Rpn5-VTENKIF

   Depositor

   Andreas Martin

   Article

   Greene et al Elife. 2019 Nov 28;8:e49806. doi: 10.755

   Insert

   rpn5 (RPN5 Budding Yeast)
   

   Use

   Tags

   3x-FLAG-HRV3C-

   Expression

   Yeast

   Mutation

   (amino acids 121-131) VTENKIF mutated to AAAAAAA

   Promoter

   Available Since

   Dec. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. TCT-Universal plasmid

   Plasmid

   #221852

   Purpose

   A universal plasmid for expressing any protein with TetraCysteine motif

   Depositor

   Artur Krężel

   Article

   Antonenko et al ACS Omega. 2024 Oct 30;9(45):45127-45137

   Insert

   Tetra Cysteine tag
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   No

   Promoter

   Available Since

   Aug. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRAR119

   Plasmid

   #185051

   Purpose

   J23108(spel), pHP14 stability hairpin, strong RBS, L34 coding region, trrnB

   Depositor

   Julius Lucks

   Article

   Rasmussen et al Nucleic Acids Res. 2022 Dec 9;50(22):127

   Insert

   L34
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   July 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. RT-5

   Plasmid

   #220442

   Purpose

   Construct used to generate the PUF3/PUP2/3HA tagging strains. (aka pBSII/PUF3 5’ flank/PUP2-DADA/3HA/URA3/PUF3 3’ flank)

   Depositor

   Marvin Wickens

   Article

   Lapointe et al Nat Methods. 2015 Dec;12(12):1163-70. do

   Insert

   PUF3 (puf-3 Nematode)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   June 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. RT-4

   Plasmid

   #220443

   Purpose

   Construct used to generate the PUF3/PUP2/3HA tagging strains. Surrounding the PUP2/3HA/URA3 are large regions of homology to integrate at the 3’ end of the PUF3 gene.

   Depositor

   Marvin Wickens

   Article

   Lapointe et al Nat Methods. 2015 Dec;12(12):1163-70. do

   Insert

   PUF3 (PUF3 Budding Yeast)
   

   Use

   Tags

   3xHA

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   June 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. HA-MBP-TEVpCS-ADGRF2 CTF-FLAG

   Plasmid

   #217695

   Purpose

   Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

   Depositor

   Stephen Blacklow

   Article

   Dates et al bioRxiv 2022.07.14.500097

   Insert

   ADGRF2 (ADGRF2 Human)
   

   Use

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   Deletion of residues 1-429

   Promoter

   Available Since

   April 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. HA-MBP-TEVpCS-ADGRE4 CTF-FLAG

   Plasmid

   #217692

   Purpose

   Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

   Depositor

   Stephen Blacklow

   Article

   Dates et al bioRxiv 2022.07.14.500097

   Insert

   ADGRE4 (ADGRE4P Human)
   

   Use

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   Deletion of residues 1-173

   Promoter

   Available Since

   April 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. HA-MBP-TEVpCS-ADGRA1-FLAG

    Plasmid

    #217678

    Purpose

    Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

    Depositor

    Stephen Blacklow

    Article

    Dates et al bioRxiv 2022.07.14.500097

    Insert

    ADGRA1 (ADGRA1 Human)
    

    Use

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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