We narrowed to 9,951 results for: Uty

1. pEXSISERS_FLuc_loxP-PuroR

   Plasmid

   #177866

   Purpose

   Cloning Backbone for Firefly-luciferase-based EXSISERS containing loxP-sites flanked PuroR cassette

   Depositor

   Gil Westmeyer

   Article

   Truong et al Nat Cell Biol. 2021 Jun;23(6):652-663. d

   Insert

   FLuc-based EXSISERS
   

   Use

   CRISPR, Cre/Lox, Luciferase, Synthetic Biology, a…

   Tags

   FLAG

   Expression

   Mammalian

   Available Since

   April 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGL3E-hGPR56 e1m promoter normal

   Plasmid

   #52298

   Purpose

   Reporter for normal human GPR56 exon 1m promoter activity. Human GPR56 e1m promoter (chr16:56,230,630-56,230,943 (hg18)) was inserted into pGL3E.

   Depositor

   Christopher A Walsh

   Article

   Bae et al Science. 2014 Feb 14;343(6172):764-8. do

   Insert

   ADGRG1 adhesion G protein-coupled receptor G1 (ADGRG1 Human)
   

   Use

   Luciferase

   Available Since

   April 15, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDONR207 SARS-CoV-2 NSP1 124A/125A

   Plasmid

   #164523

   Purpose

   Gateway-compatible Entry vector, with insert of mutated NSP1 CDS from SARS-CoV-2 isolate Wuhan-Hu-1 (amino acid substitutions R124A/K125A)

   Depositor

   Mitchell Guttman

   Article

   Banerjee et al Cell. 2020 Nov 25;183(5):1325-1339.e21.

   Insert

   NSP1 124A/125A (ORF1ab )
   

   Expression

   Mammalian

   Mutation

   Insert of NSP1 gene's CDS from SARS-CoV-2 is…

   Available Since

   Feb. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLKO.1-TRC.mKO2_shGLS

   Plasmid

   #110319

   Purpose

   shGLS (Target CAACTGGCCAAATTCAGTC), silence human GLS gene and express monomeric Kusabira-Orange2

   Depositor

   Sandra Martha Gomes Dias

   Article

   Quintero et al BMC Cancer. 2017 Nov 7;17(1):727. doi: 1

   Insert

   GLS Glutaminase (GLS Human)
   

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Promoter

   RNA polymerase III promoter for human U6 snRNA fo…

   Available Since

   Nov. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLKO-PHB-6

   Plasmid

   #122232

   Purpose

   Expresses shRNA targeting the coding sequence of human PHB

   Depositor

   Alex Kentsis

   Article

   Ortiz et al JCI Insight. 2019 Aug 8;4(15). pii: 1270

   Insert

   shRNA targeting PHB (see partial depositor seq) (PHB1 Human)
   

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Available Since

   Aug. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEMS1401

   Plasmid

   #29198

   Purpose

   High copy homologous recombination plasmid for gene targeting at the mouse Hprt locus containing Ple67 (FEV MiniPromoter) driving an EGFP reporter

   Depositor

   Elizabeth Simpson

   Article

   Korecki et al Genetics. 2019 Feb 14. pii: genetics.119

   Insert

   Ple67 (FEV Human)
   

   Use

   Pleiades promoter project [sic, pleaides plieades]

   Tags

   EGFP-NLS

   Available Since

   Nov. 15, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGG369

   Plasmid

   #165606

   Purpose

   Vector for expression of ω-dCas9 and sgRNA in E. coli: lacUV2-ω-1xFLAG-dCas9(Wt PID)-1xNLS-3xHA-1xNLS and UV5-BsaI-sgRNA (BsaI sites in place of the spacer)

   Depositor

   Marcus Noyes

   Article

   Goldberg et al Nat Commun. 2021 Jan 13;12(1):349. doi:

   Insert

   ω-1xFLAG-dCas9(PAM-interacting domain and overall coding scheme from Wt SpCas9)-1xNLS-3xHA-1xNLS and BsaI-sgRNA
   

   Use

   CRISPR and Synthetic Biology

   Tags

   1x FLAG, 3x HA, Omega (ω) subunit of RNA polymera…

   Expression

   Bacterial

   Mutation

   Recoding of BsaI site in the AmpR cassette, two B…

   Promoter

   lacUV2 driving ω-dCas9 and UV5 driving sgRNA

   Available Since

   April 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLentiCMVblast_SLC38A2_N82A

   Plasmid

   #156181

   Purpose

   CMV-driven expression of sgRNA-resistant SLC38A2 N82A mutant cDNA.

   Depositor

   Alec Kimmelman

   Article

   Parker et al Cancer Discov. 2020 Apr 27. pii: 2159-82

   Insert

   SLC38A2 (SLC38A2 Human)
   

   Expression

   Mammalian

   Mutation

   Silent mutations to prevent targeting by sgRNA: T…

   Promoter

   CMV

   Available Since

   Aug. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pInducer20_SLC38A2_N82A

   Plasmid

   #156183

   Purpose

   Dox-inducible expression of sgRNA-resistant SLC38A2 N82A mutant cDNA.

   Depositor

   Alec Kimmelman

   Article

   Parker et al Cancer Discov. 2020 Apr 27. pii: 2159-82

   Insert

   SLC38A2 (SLC38A2 Human)
   

   Expression

   Mammalian

   Mutation

   Silent mutations to prevent targeting by sgRNA: T…

   Promoter

   TRE

   Available Since

   Aug. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. JDW 822 (pDONR221-V5-mScarlet-I-KRAS4A-WT)

    Plasmid

    #156405

    Purpose

    Gateway middle entry clone encoding V5-tagged mScarlet-I-fused to human KRAS4A, includes a stop codon

    Depositor

    Joshua Wythe

    Article

    Fish et al Circ Res. 2020 Jun 17. doi: 10.1161/CIRC

    Insert

    human wild type KRAS4A-WT (KRAS Human)
    

    Use

    Gateway cloning

    Tags

    mScarlet-I

    Available Since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA5 rtTA BirA eIF4G

    Plasmid

    #158789

    Purpose

    pcDNA5 based transfection plasmid for the exogenous expression of N-terminal BirA-Flag-tagged eIF4G1 lacking the microexon for generation of N2A FlpIn cell lines for BioID

    Depositor

    Benjamin Blencowe

    Article

    Gonatopoulos-Pournatzis et al Mol Cell. 2020 Mar 19;77(6):1176-1192.e1

    Insert

    eIF4G1
    

    Tags

    3xFlag and BirA*

    Expression

    Mammalian

    Promoter

    miniCMV

    Available Since

    Aug. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGL3E-hGPR56 e1m promoter perisylvian polymicrogyria

    Plasmid

    #52299

    Purpose

    Reporter for mutated human GPR56 exon 1m promoter activity. It contains a 15-bp deletion in a conserved noncoding element. The mutated human GPR56 e1m promoter was inserted into pGL3E.

    Depositor

    Christopher A Walsh

    Article

    Bae et al Science. 2014 Feb 14;343(6172):764-8. do

    Insert

    ADGRG1 adhesion G protein-coupled receptor G1 (ADGRG1 Human)
    

    Use

    Luciferase

    Mutation

    15-bp deletion in the promoter

    Promoter

    Human GPR56 e1m promoter

    Available Since

    April 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pEMS1538

    Plasmid

    #29261

    Purpose

    High copy homologous recombination plasmid for gene targeting at the mouse Hprt locus containing Ple67 (FEV MiniPromoter) driving a lacZ reporter

    Depositor

    Elizabeth Simpson

    Article

    Korecki et al Genetics. 2019 Feb 14. pii: genetics.119

    Insert

    Ple67 (FEV Human)
    

    Use

    Pleiades promoter project [sic, pleaides plieades]

    Tags

    Intron-LacZ-NLS

    Available Since

    Oct. 28, 2011

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pTW334e2b

    Plasmid

    #246587

    Purpose

    sequential knock out of A. thaliana Rubisco assembly factors

    Depositor

    Tina Wang

    Article

    Li et al Proc Natl Acad Sci U S A. 2025 Sep 16;12

    Insert

    AtRaf1, AtRaf2, AtRbcX2, AtBSD2W108A/L109E, AtCpn60alpha, AtCpn60beta, AtCpn20 (AT3G04550, CPN60B, CPN60A, CPN20, RbcX2 Mustard Weed)
    

    Expression

    Bacterial

    Mutation

    AtBSD2W108A/L109E

    Available Since

    Oct. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTW334e4

    Plasmid

    #246589

    Purpose

    sequential knock out of A. thaliana Rubisco assembly factors

    Depositor

    Tina Wang

    Article

    Li et al Proc Natl Acad Sci U S A. 2025 Sep 16;12

    Insert

    AtRaf1, AtRbcX2, AtBSD2, AtCpn60alpha, AtCpn60beta, AtCpn20 (AT3G04550, CPN20, RbcX2, CPN60B, CPN60A Mustard Weed)
    

    Expression

    Bacterial

    Available Since

    Oct. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTW334e2

    Plasmid

    #246586

    Purpose

    sequential knock out of A. thaliana Rubisco assembly factors

    Depositor

    Tina Wang

    Article

    Li et al Proc Natl Acad Sci U S A. 2025 Sep 16;12

    Insert

    AtRaf1, AtRaf2, AtRbcX2, AtCpn60alpha, AtCpn60beta, AtCpn20 (AT3G04550, CPN60B, CPN60A, CPN20, RbcX2 Mustard Weed)
    

    Expression

    Bacterial

    Available Since

    Oct. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDMJ2-SARS-CoV-2-Spike (B.1-10c)

    Plasmid

    #241036

    Purpose

    Expresses SARS-CoV-2 spike (B.1-10c) protein for creating pseudotyped virus

    Depositor

    Jeremy Luban

    Article

    Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

    Insert

    Spike (B.1-10c) (S SARS-CoV-2)
    

    Expression

    Mammalian

    Mutation

    T76R,K113T,K147N,S155R,N439R,T478K,E484V,S494R,K5…

    Available Since

    Sept. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pDMJ2-SARS-CoV-2-Spike (Gamma)

    Plasmid

    #241115

    Purpose

    Expresses SARS-CoV-2 spike (Gamma) protein for creating pseudotyped virus

    Depositor

    Jeremy Luban

    Article

    Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

    Insert

    Spike (Gamma) (S SARS-CoV-2)
    

    Expression

    Mammalian

    Mutation

    L18F,T20N,P26S,D138Y,R190S,K417T,E484K,N501Y,D614…

    Available Since

    Sept. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDMJ2-SARS-CoV-2-Spike (Delta)

    Plasmid

    #241113

    Purpose

    Expresses SARS-CoV-2 spike (Delta) protein for creating pseudotyped virus

    Depositor

    Jeremy Luban

    Article

    Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

    Insert

    Spike (Delta) (S SARS-CoV-2)
    

    Expression

    Mammalian

    Mutation

    T19R,G142D,E156G,F157del,R158del,L452R,T478K,D614…

    Available Since

    Sept. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pDMJ2-SARS-CoV-2-Spike (Beta)

    Plasmid

    #241109

    Purpose

    Expresses SARS-CoV-2 spike (Beta) protein for creating pseudotyped virus

    Depositor

    Jeremy Luban

    Article

    Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

    Insert

    Spike (Beta) (S SARS-CoV-2)
    

    Expression

    Mammalian

    Mutation

    L18F,D80A,D215G,L241del,L242del,A243del,K417N,E48…

    Available Since

    Sept. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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