We narrowed to 59,355 results for: cloning

1. pMINTC3GH (Kan)

   Plasmid

   #110076

   Purpose

   Analogous to pMINTNH3 but with a C-terminal 3C cleavage site and a GFP-His10 tag fusion.

   Depositor

   Markus Seeger

   Article

   Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

   Type

   Empty backbone

   Tags

   3C cleavage site - GFP - His10 Tag

   Expression

   Bacterial

   Available Since

   June 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMAD-Gate2

   Plasmid

   #25941

   Depositor

   Kamil Onder

   Article

   Maier et al Assay Drug Dev Technol. 2010 Oct;8(5):62

   Insert

   
   

   Tags

   VP16 Fusion Protein

   Expression

   Mammalian

   Available Since

   Aug. 5, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJRA-GW

   Plasmid

   #209333

   Purpose

   Gateway cloning compatible binary vector for agrobacterium mediated transformation. MCS upstream of gateway.

   Depositor

   Lucia Strader

   Article

   Allen et al BMC Res Notes. 2025 Apr 8;18(1):149. doi

   Type

   Empty backbone

   Expression

   Plant

   Available Since

   Jan. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. plIVMD156

   Plasmid

   #160934

   Purpose

   In vivo mRNA display Gateway Destination construct

   Depositor

   Saeed Tavazoie

   Article

   Oikonomou et al Proc Natl Acad Sci U S A. 2020 Oct 9. pi

   Insert

   MCP-Gateway cloning site-HIS tag, 3'UTR MS2 Stem Loop
   

   Tags

   HIS tag, 3'UTR MS2 Stem Loop

   Expression

   Yeast

   Promoter

   MET25

   Available Since

   Jan. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pM115: CasRx control presgRNA

   Plasmid

   #166867

   Purpose

   U6-driven expression of control (non-targeting) presgRNA compatible with CasRx.

   Depositor

   Guei-Sheung Liu

   Article

   Chuang et al Front Cell Dev Biol. 2021 Jun 11;9:66787

   Insert

   non-targeting presgRNA
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   July 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMC-X4

   Plasmid

   #176154

   Purpose

   preassembled yeast MoClo level-2 backbone with GFP dropout for genomic integration in site X-4

   Depositor

   Florian David

   Article

   Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

   Type

   Empty backbone

   Expression

   Yeast

   Available Since

   Feb. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMC-XII1

   Plasmid

   #176159

   Purpose

   preassembled yeast MoClo level-2 backbone with GFP dropout for genomic integration in site XII-1

   Depositor

   Florian David

   Article

   Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

   Type

   Empty backbone

   Expression

   Yeast

   Available Since

   Feb. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMC-XI3

   Plasmid

   #176157

   Purpose

   preassembled yeast MoClo level-2 backbone with GFP dropout for genomic integration in site XI-3

   Depositor

   Florian David

   Article

   Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

   Type

   Empty backbone

   Expression

   Yeast

   Available Since

   Feb. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. p005kanRFP

   Plasmid

   #58533

   Purpose

   RFP from coral Corynactis sp. under T5 promoter with lac operators; kanamycin resistant.

   Depositor

   Jaroslaw Bryk

   Article

   Siddall et al bioRxiv 2021.06.20.449138

   Insert

   Red fluorescent protein
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   T5 between two lac operators

   Available Since

   Oct. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMC-XII5

    Plasmid

    #176161

    Purpose

    preassembled yeast MoClo level-2 backbone with GFP dropout for genomic integration in site XII-5

    Depositor

    Florian David

    Article

    Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

    Type

    Empty backbone

    Expression

    Yeast

    Available Since

    Feb. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pICSL50015

    Plasmid

    #50318

    Purpose

    Please note- this plasmid is currently not distributed in the MoClo Plant Parts Kit (# 1000000047)

    Depositor

    Nicola Patron

    Article

    Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

    Insert

    CDS, mNEON (Branchistoma lanceolatum)
    

    Use

    Plant expression

    Mutation

    BsaI/BbsI sites removed by point-mutation

    Available Since

    Feb. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFH113

    Plasmid

    #128210

    Purpose

    improved sgRNA backbone for tRNA-gRNA array, Position 1, combine with TaU3 promoter module (pFH31)

    Depositor

    Vladimir Nekrasov

    Article

    Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

    Insert

    improved sgRNA backbone for tRNA-gRNA array, Position 1, combine with TaU3 promoter module (pFH31)
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Plant

    Available Since

    Aug. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pME-TagRFPt-NS

    Plasmid

    #75343

    Purpose

    Multisite gateway entry clone for adding N-terminal fusions of TagRFPt

    Depositor

    Rob Parton

    Article

    Don et al Zebrafish. 2016 Sep 15.

    Insert

    TagRFPt
    

    Expression

    Mammalian

    Available Since

    Aug. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pUC57-Kan_BR29_00091361

    Plasmid

    #121209

    Purpose

    Level 0 Golden-Gate compatible vector

    Depositor

    Sophien Kamoun

    Article

    Petit-Houdenot et al Mol Plant Microbe Interact. 2020 Aug;33(

    Insert

    BR29_00091361
    

    Use

    Golden gate

    Mutation

    Magnaporthe grisea

    Available Since

    March 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJC225

    Plasmid

    #176883

    Purpose

    MoClo Level1 plasmid, allows transition into Cermak, et al., 2017 GG cloning system

    Depositor

    Daniel Voytas

    Article

    Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

    Type

    Empty backbone

    Use

    Cloning

    Available Since

    May 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. p663-UBC-miniTurbo-V5-ERN2_IDG-K

    Plasmid

    #189905

    Purpose

    Gateway destination clone of ERN2 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    ERN2 (ERN2 Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    miniTurbo-V5

    Expression

    Mammalian

    Promoter

    Ubiquitin

    Available Since

    Dec. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pJRA103

    Plasmid

    #209336

    Purpose

    Gateway cloning compatible binary vector for agrobacterium mediated transformation. Arabidopsis UBQ10 promoter expression of N-terminal SNAP-tag fusion protein.

    Depositor

    Lucia Strader

    Article

    Allen et al BMC Res Notes. 2025 Apr 8;18(1):149. doi

    Type

    Empty backbone

    Expression

    Plant

    Available Since

    Jan. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pME-nlsGal80(opt)

    Plasmid

    #51618

    Purpose

    vertebrate expression of codon-optimized Gal80 with N-terminal NLS and no stop

    Depositor

    Joshua Bonkowsky

    Article

    Fujimoto et al Dev Dyn. 2011 Oct;240(10):2324-34. doi:

    Insert

    Gal80(opt) (GAL80 Budding Yeast)
    

    Use

    Vertebrate expression

    Tags

    NLS

    Mutation

    codon optimized

    Available Since

    May 19, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pF hPGK mc2 IRES neo

    Plasmid

    #206236

    Purpose

    For expressing cloned exons as circular RNA.

    Depositor

    Simon Conn, Brett Stringer

    Article

    Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

    Type

    Empty backbone

    Use

    Lentiviral and Synthetic Biology

    Expression

    Mammalian

    Available Since

    Sept. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGL4.23-SCP1-ccdB

    Plasmid

    #131628

    Purpose

    Modified STARR-seq plasmid to measure enhancer activity (SCP1 promoter; ccdb gene at cloning site)

    Depositor

    Kevin White

    Article

    Liu et al Genome Biol. 2017 Nov 20;18(1):219. doi:

    Type

    Empty backbone

    Use

    Luciferase

    Expression

    Bacterial and Mammalian

    Promoter

    SCP1

    Available Since

    Oct. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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