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plentiCRISPRv2 ERBIN

Plasmid

#258467

Purpose

CRISPR-mediated knock-out of human ERBIN

Depositor

Matthew Kutys

Publication

Mayo et al J Cell Biol. 2026 Jun 1;225(6):e20250314

Insert

ERBIN sgRNA

Use

Lentiviral

Expression

Mammalian

Promoter

U6

Available since

July 22, 2026

Availability

Academic Institutions and Nonprofits only

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plentiCRISPRv2 DLG5

Plasmid

#258468

Purpose

CRISPR-mediated knock-out of human DLG5

Depositor

Matthew Kutys

Publication

Mayo et al J Cell Biol. 2026 Jun 1;225(6):e20250314

Insert

DLG5 sgRNA

Use

Lentiviral

Expression

Mammalian

Promoter

U6

Available since

July 22, 2026

Availability

Academic Institutions and Nonprofits only

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pLJSRSF7-T7-6xHis-SUMO-MRT-CBD

Plasmid

#253350

Purpose

For the expression of group II intron encoded reverse transcriptase MarathonRT with N-terminal SUMO-tag and C-terminal CBD-tag in E. coli.

Depositor

Peter Sarin

Publication

Pedor et al Bio Protoc. 2026 Jun 20;16(12):e5703. do

Insert

6xHis-SUMO-MarathonRT with added C-terminal CBD-tag originating from the backbone.

Tags

6xHis-SUMO and CBD

Expression

Bacterial

Promoter

T7

Available since

May 11, 2026

Availability

Academic Institutions and Nonprofits only

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pGIPZ EF1a spnull-3xFLAG-DR18 IRES mNeonGreen-P2A-Puro

Plasmid

#248037

Purpose

Constitutively expresses 3xFLAG tagged DR18, mNeonGreen and a puromycin resistance gene in human cells

Depositor

Joshua Leonard

Publication

Deol et al GEN Biotechnol. 2023 Jun 1;2(3):228-246.

Insert

3xFLAG-tagged DR18

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Promoter

human EF1a

Available since

Jan. 16, 2026

Availability

Academic Institutions and Nonprofits only

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pGIPZ EF1a ACCx2 uORF eIL12 IRES mNeonGreen-P2A-Puro

Plasmid

#248035

Purpose

Constitutively expresses an engineered version of IL12, mNeonGreen and a puromycin resistance gene in human cells

Depositor

Joshua Leonard

Publication

Deol et al GEN Biotechnol. 2023 Jun 1;2(3):228-246.

Insert

Engineered IL12

Use

Lentiviral

Expression

Mammalian

Promoter

human EF1a

Available since

Jan. 16, 2026

Availability

Academic Institutions and Nonprofits only

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pCMV-Gpr45

Plasmid

#245982

Purpose

Express wild-type mouse GPR45 transiently via transfection in mammalian cells.

Depositor

Zhao Zhang

Publication

Xun et al Science. 2025 Jun 5;388(6751):eadp3989.

Insert

Gpr45 (Gpr45 Mouse)

Expression

Mammalian

Promoter

CMV

Available since

Oct. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-Gpr45-Y287C

Plasmid

#245983

Purpose

Express mouse GPR45-Y287C mutant transiently via transfection in mammalian cells.

Depositor

Zhao Zhang

Publication

Xun et al Science. 2025 Jun 5;388(6751):eadp3989.

Insert

Gpr45 (Gpr45 Mouse)

Expression

Mammalian

Mutation

Y287C

Promoter

CMV

Available since

Oct. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-Gpr45-S214P

Plasmid

#245984

Purpose

Express mouse GPR45-S214P mutant transiently via transfection in mammalian cells.

Depositor

Zhao Zhang

Publication

Xun et al Science. 2025 Jun 5;388(6751):eadp3989.

Insert

Gpr45 (Gpr45 Mouse)

Expression

Mammalian

Mutation

S214P

Promoter

CMV

Available since

Oct. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pSCT376: pTwinStrep-bdSUMO-ZooTsy_TnsF-Y584F

Plasmid

#200877

Purpose

ZooTsy_TnsF expression construct for protein purification.

Depositor

Feng Zhang

Publication

Faure et al Mol Cell. 2023 Jun 15;83(12):2122-2136.e

Insert

ZooTsy_TnsF

Expression

Bacterial

Mutation

TnsF-Y584F

Available since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

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pSCT221: pR6K-Donor(ZooTsy)_H1:12_E1:135_E2:20_H2:12

Plasmid

#200874

Purpose

ZooTsy donor harboring KanR cargo.

Depositor

Feng Zhang

Publication

Faure et al Mol Cell. 2023 Jun 15;83(12):2122-2136.e

Insert

ZooTsy

Expression

Bacterial

Available since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

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pSCT384: pSC101-Donor(ZooTsy)

Plasmid

#200875

Purpose

ZooTsy donor harboring KanR cargo.

Depositor

Feng Zhang

Publication

Faure et al Mol Cell. 2023 Jun 15;83(12):2122-2136.e

Insert

ZooTsy

Expression

Bacterial

Available since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

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pSCT81: pTarget(ZooTsy)

Plasmid

#200876

Purpose

Target plasmid containing attachement site for ZooTsy.

Depositor

Feng Zhang

Publication

Faure et al Mol Cell. 2023 Jun 15;83(12):2122-2136.e

Insert

ZooTsy_comM

Expression

Bacterial

Available since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

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pSCT315: pHelper(ZooTsy)_TnsF-Y584F

Plasmid

#200872

Purpose

Inducible expression of ZooTsy proteins.

Depositor

Feng Zhang

Publication

Faure et al Mol Cell. 2023 Jun 15;83(12):2122-2136.e

Insert

ZooTsy

Expression

Bacterial

Mutation

TnsF-Y584F

Available since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

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pSCT155: pHelper(ZooTsy)_ΔHTH

Plasmid

#200868

Purpose

Inducible expression of ZooTsy (except HTH) proteins.

Depositor

Feng Zhang

Publication

Faure et al Mol Cell. 2023 Jun 15;83(12):2122-2136.e

Insert

ZooTsy

Expression

Bacterial

Available since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

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pSCT156: pHelper(ZooTsy)_ΔTnsF

Plasmid

#200869

Purpose

Inducible expression of ZooTsy (except TnsF) proteins.

Depositor

Feng Zhang

Publication

Faure et al Mol Cell. 2023 Jun 15;83(12):2122-2136.e

Insert

ZooTsy

Expression

Bacterial

Available since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

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pSCT148: pHelper(ZooTsy)

Plasmid

#200866

Purpose

Inducible expression of ZooTsy proteins.

Depositor

Feng Zhang

Publication

Faure et al Mol Cell. 2023 Jun 15;83(12):2122-2136.e

Insert

ZooTsy

Expression

Bacterial

Available since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

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pSCT154: pHelper(ZooTsy)_ΔYRec

Plasmid

#200867

Purpose

Inducible expression of ZooTsy (except YRec) proteins.

Depositor

Feng Zhang

Publication

Faure et al Mol Cell. 2023 Jun 15;83(12):2122-2136.e

Insert

ZooTsy

Expression

Bacterial

Available since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

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pSCT368: pSC101-Donor(AjTn6022)

Plasmid

#200864

Purpose

AjTn6022 donor harboring KanR cargo.

Depositor

Feng Zhang

Publication

Faure et al Mol Cell. 2023 Jun 15;83(12):2122-2136.e

Insert

AjTn6022

Expression

Bacterial

Available since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

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pSCT401: pHelper(AjTn6022)_QF-separated_TniQ-K361D

Plasmid

#200861

Purpose

Inducible expression of AjTn6022 proteins.

Depositor

Feng Zhang

Publication

Faure et al Mol Cell. 2023 Jun 15;83(12):2122-2136.e

Insert

AjTn6022

Expression

Bacterial

Mutation

TniQ-K361D

Available since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

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pSCT112: pTarget(AjTn6022)_U50D50

Plasmid

#200862

Purpose

Target plasmid containing attachement site for AjTn6022.

Depositor

Feng Zhang

Publication

Faure et al Mol Cell. 2023 Jun 15;83(12):2122-2136.e

Insert

AjTn6022_comM

Expression

Bacterial

Available since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

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