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Addgene

We narrowed to 9,384 results for: Coli

Showing: 5961 - 5980 of 9384 results
  1. pJB75

    Plasmid
    #226320
    Purpose
    E. coli protein expression for protein purification
    Depositor
    Insert
    6xHis-(Y to A MR1-6)-strep
    Use
    Tags
    6xHis and Strep
    Expression
    Bacterial
    Mutation
    N-terminal domain deleted, C-terminal domain dele…
    Promoter
    T7
    Available since
    Oct. 22, 2024
    Availability
    Academic Institutions and Nonprofits only
  2. pJB67

    Plasmid
    #226316
    Purpose
    E. coli and H. neapolitanus shuttle vector; integrates the gene of interest and a kanamycin resistance marker into a neutral site on the H. neapolitanus genome.
    Depositor
    Insert
    CsoS2 with K to A CsoS2 MR1-6
    Use
    Tags
    Expression
    Bacterial
    Mutation
    K261A, K320A, K380A, K436A, K495A, K546A
    Promoter
    pTRC
    Available since
    Oct. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
  3. pJB74

    Plasmid
    #226314
    Purpose
    E. coli and H. neapolitanus shuttle vector; integrates the gene of interest and a kanamycin resistance marker into a neutral site on the H. neapolitanus genome.
    Depositor
    Insert
    CsoS2 with Y to A CsoS2 MR1-5
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Y300A, Y359A, Y420A, Y475A, Y534A
    Promoter
    pTRC
    Available since
    Oct. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
  4. pJB73

    Plasmid
    #226313
    Purpose
    E. coli and H. neapolitanus shuttle vector; integrates the gene of interest and a kanamycin resistance marker into a neutral site on the H. neapolitanus genome.
    Depositor
    Insert
    CsoS2 with Y to A CsoS2 MR1-3
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Y300A, Y359A, Y420A
    Promoter
    pTRC
    Available since
    Oct. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
  5. pJB72

    Plasmid
    #226312
    Purpose
    E. coli and H. neapolitanus shuttle vector; integrates the gene of interest and a kanamycin resistance marker into a neutral site on the H. neapolitanus genome.
    Depositor
    Insert
    CsoS2 with Y to A CsoS2 MR1
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Y300A
    Promoter
    pTRC
    Available since
    Oct. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
  6. pJB45

    Plasmid
    #226304
    Purpose
    E. coli and H. neapolitanus shuttle vector; integrates the gene of interest and a kanamycin resistance marker into a neutral site on the H. neapolitanus genome.
    Depositor
    Insert
    CsoS2 with C to S CsoS2 MR1-2
    Use
    Tags
    Expression
    Bacterial
    Mutation
    C292S, C310S, C351S, C369S
    Promoter
    pTRC
    Available since
    Oct. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
  7. pJB44

    Plasmid
    #226303
    Purpose
    E. coli and H. neapolitanus shuttle vector; integrates the gene of interest and a kanamycin resistance marker into a neutral site on the H. neapolitanus genome.
    Depositor
    Insert
    CsoS2 with C to S MR1
    Use
    Tags
    Expression
    Bacterial
    Mutation
    C292S, C310S
    Promoter
    pTRC
    Available since
    Oct. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
  8. pJB43

    Plasmid
    #226302
    Purpose
    E. coli and H. neapolitanus shuttle vector; integrates the gene of interest and a kanamycin resistance marker into a neutral site on the H. neapolitanus genome.
    Depositor
    Insert
    WT CsoS2, frameshift site intact
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    pTRC
    Available since
    Oct. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
  9. pET28-RPL3-1~41-mEGFP(A206K)-TwStrep

    Plasmid
    #208628
    Purpose
    for E. coli expression of truncated human RPL3 (ENST00000216146), aa1~41, fused with mEGFP(A206K) and Twin Strep tag
    Depositor
    Insert
    human RPL3 (ENST00000216146) aa 1~41 (RPL3 Human)
    Use
    Tags
    6xHis, Twin Strep, and mEGFP(A206K)
    Expression
    Bacterial
    Mutation
    only aa 1~41, other residues not included
    Promoter
    T7
    Available since
    May 15, 2024
    Availability
    Academic Institutions and Nonprofits only
  10. pET28-RPL3-72~197-mEGFP(A206K)-TwStrep

    Plasmid
    #208625
    Purpose
    for E. coli expression of truncated human RPL3 (ENST00000216146), aa72~197, fused with mEGFP(A206K) and Twin Strep tag
    Depositor
    Insert
    human RPL3 (ENST00000216146) aa 72~197 (RPL3 Human)
    Use
    Tags
    6xHis, Twin Strep, and mEGFP(A206K)
    Expression
    Bacterial
    Mutation
    only aa 72~197, other residues not included
    Promoter
    T7
    Available since
    May 15, 2024
    Availability
    Academic Institutions and Nonprofits only
  11. pET-6xHis-GFP(+6)-L1

    Plasmid
    #205039
    Purpose
    Fluorescent protein with charge-patterned cationic peptide to promote biomolecular condensate formation in E. coli
    Depositor
    Insert
    GFP(+6)-L1
    Use
    Tags
    GRRGKKSRK and MGHHHHHGGA
    Expression
    Bacterial
    Mutation
    E16R, D86K, D112K, D127R, I138R, D207R, N222K, L2…
    Promoter
    Available since
    Feb. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
  12. pET-6xHis-GFP(+6)-L2

    Plasmid
    #205040
    Purpose
    Fluorescent protein with charge-patterned cationic peptide to promote biomolecular condensate formation in E. coli
    Depositor
    Insert
    GFP(+6)-L2
    Use
    Tags
    GRRGKKSRKGRRGKKSRK and MGHHHHHGGA
    Expression
    Bacterial
    Mutation
    E16R, D86K, D112K, D127R, I138R, D207R, N222K, L2…
    Promoter
    Available since
    Feb. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
Showing: 5961 - 5980 of 9384 results