Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 23,305 results for: Sis

Showing: 11921 - 11960 of 23305 results

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pDGE500

Plasmid

#153273

Purpose

M6E shuttle vector containing the Solanum lycopersicum U3 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

Depositor

Johannes Stuttmann

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pDGE502

Plasmid

#153275

Purpose

M8E shuttle vector containing the Solanum lycopersicum U3 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

Depositor

Johannes Stuttmann

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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Lenti-Myosin7B-sgRNA

Plasmid

#154861

Purpose

Lentiviral expression of Cas9 and sgRNA targeting Myosin7B

Depositor

Yihong Ye

Article

Zhang et al Proc Natl Acad Sci U S A. 2020 May 19;11

Insert

MYO7B sgRNA (MYO7B Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

July 27, 2020

Availability

Academic Institutions and Nonprofits only

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pMAL-C2X dAmp/Kana+-2MT

Plasmid

#154353

Purpose

Bacterial expression of 2MT

Depositor

Wanzhong He

Article

Jiang et al Nat Methods. 2020 Sep;17(9):937-946. doi

Insert

2MT

Tags

His

Expression

Bacterial

Available Since

July 24, 2020

Availability

Academic Institutions and Nonprofits only

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pDUAL-P41nmt1-Sad1-GFP

Plasmid

#154366

Purpose

Yeast expression of Sad1

Depositor

Wanzhong He

Article

Jiang et al Nat Methods. 2020 Sep;17(9):937-946. doi

Insert

Sad1 (sad1 Fission Yeast)

Expression

Yeast

Available Since

July 24, 2020

Availability

Academic Institutions and Nonprofits only

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pDUAL-P41nmt1-Nup124-GFP

Plasmid

#154364

Purpose

Yeast expression of Nup124

Depositor

Wanzhong He

Article

Jiang et al Nat Methods. 2020 Sep;17(9):937-946. doi

Insert

Nup124 (nup124 Fission Yeast)

Expression

Yeast

Available Since

July 23, 2020

Availability

Academic Institutions and Nonprofits only

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pDUAL-Pnmt1-Ost4-GFP-MTalpha

Plasmid

#154370

Purpose

Yeast expression of Ost4

Depositor

Wanzhong He

Article

Jiang et al Nat Methods. 2020 Sep;17(9):937-946. doi

Insert

Ost4 (ost4 Fission Yeast)

Expression

Yeast

Available Since

July 23, 2020

Availability

Academic Institutions and Nonprofits only

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pDUAL-P41nmt1-Sad1-GFP-MTn

Plasmid

#154367

Purpose

Yeast expression of Sad1

Depositor

Wanzhong He

Article

Jiang et al Nat Methods. 2020 Sep;17(9):937-946. doi

Insert

Sad1 (sad1 Fission Yeast)

Expression

Yeast

Available Since

July 23, 2020

Availability

Academic Institutions and Nonprofits only

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pRSFDuet1-tglA(C51)HIB

Plasmid

#134675

Purpose

Expresses His6-TglA(C51), TglH, TglI and TglB in E. Coli

Depositor

Wilfred van der Donk

Article

Ting et al Science. 2019 Jul 19;365(6450):280-284.

Inserts

TglA(C51)
TglH
TglI
TglB

Tags

His6

Expression

Bacterial

Mutation

insertion of cysteine 51

Promoter

T7

Available Since

July 17, 2020

Availability

Academic Institutions and Nonprofits only

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pFloat.assemble(AB).LC_v1

Plasmid

#133083

Purpose

Tier 1 destination vector for assembling gene cassettes intended for expression in bacteria. AB sites. Low copy. Version 1.0.

Depositor

Kacper Rogala, David Sabatini

Article

Rogala et al Science. 2019 Oct 25;366(6464):468-475.

Type

Empty backbone

Use

Destination vector

Expression

Bacterial

Available Since

May 26, 2020

Availability

Academic Institutions and Nonprofits only

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pFloat.assemble(AB).HC_v1

Plasmid

#133084

Purpose

Tier 1 destination vector for assembling gene cassettes intended for expression in bacteria. AB sites. High copy. Version 1.0.

Depositor

Kacper Rogala, David Sabatini

Article

Rogala et al Science. 2019 Oct 25;366(6464):468-475.

Type

Empty backbone

Use

Destination vector

Expression

Bacterial

Available Since

May 26, 2020

Availability

Academic Institutions and Nonprofits only

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pCB6-Occludin

Plasmid

#136637

Purpose

Expression of Occludin in mammalian cells

Depositor

Maria Balda, Karl Matter

Article

Balda et al J Cell Biol. 1996 Aug;134(4):1031-49. do

Insert

Occludin (OCLN Chicken)

Expression

Mammalian

Available Since

March 2, 2020

Availability

Academic Institutions and Nonprofits only

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pCB6-HAOccludin

Plasmid

#136638

Purpose

Expression of HAOccludin in mammalian cells

Depositor

Maria Balda, Karl Matter

Article

Balda et al J Cell Biol. 1996 Aug;134(4):1031-49. do

Insert

HAOccludin (OCLN Chicken)

Tags

HA (MQDLPGNDNSTAG)

Expression

Mammalian

Available Since

March 2, 2020

Availability

Academic Institutions and Nonprofits only

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pCB6-OccludinCT3

Plasmid

#136639

Purpose

Expression of OccludinCT3 in mammalian cells

Depositor

Maria Balda, Karl Matter

Article

Balda et al J Cell Biol. 1996 Aug;134(4):1031-49. do

Insert

OccludinCT3 (OCLN Chicken)

Expression

Mammalian

Mutation

Deletion of C-terminal cytoplasid domain

Available Since

March 2, 2020

Availability

Academic Institutions and Nonprofits only

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pCB6-HAOccludinCT3

Plasmid

#136640

Purpose

Expression of HAOccludinCT3 in mammalian cells

Depositor

Maria Balda, Karl Matter

Article

Balda et al J Cell Biol. 1996 Aug;134(4):1031-49. do

Insert

HAOccludinCT3 (OCLN Chicken)

Tags

HA (MQDLPGNDNSTAG)

Expression

Mammalian

Mutation

Deletion of C-terminal cytoplasid domain

Available Since

March 2, 2020

Availability

Academic Institutions and Nonprofits only

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pY71-tet.I.-1

Plasmid

#133539

Purpose

sfGFP driven by a T7 promoter with the tetO sequence 36 bp upstream from the promoter start site

Depositor

Richard Murray

Article

McManus et al Arch Biochem Biophys. 2019 Jul 18. pii:

Insert

sfGFP

Tags

Strep tag and TEV site

Expression

Bacterial

Mutation

WT

Promoter

T7

Available Since

Dec. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pY71-tet.I.25

Plasmid

#133538

Purpose

sfGFP driven by a T7 promoter with the tetO sequence 28 bp downstream from the promoter start site

Depositor

Richard Murray

Article

McManus et al Arch Biochem Biophys. 2019 Jul 18. pii:

Insert

sfGFP

Tags

Strep tag and TEV site

Expression

Bacterial

Mutation

WT

Promoter

T7

Available Since

Dec. 20, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pY71-tet.I.20

Plasmid

#133537

Purpose

sfGFP driven by a T7 promoter with the tetO sequence 23 bp downstream from the promoter start site

Depositor

Richard Murray

Article

McManus et al Arch Biochem Biophys. 2019 Jul 18. pii:

Insert

sfGFP

Tags

Strep tag and TEV site

Expression

Bacterial

Mutation

WT

Promoter

T7

Available Since

Dec. 20, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pY71-tet.I.14

Plasmid

#133535

Purpose

sfGFP driven by a T7 promoter with the tetO sequence 17 bp downstream from the promoter start site

Depositor

Richard Murray

Article

McManus et al Arch Biochem Biophys. 2019 Jul 18. pii:

Insert

sfGFP

Tags

Strep tag and TEV site

Expression

Bacterial

Mutation

WT

Promoter

T7

Available Since

Dec. 20, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pY71-tet.I.13

Plasmid

#133534

Purpose

sfGFP driven by a T7 promoter with the tetO sequence 16 bp downstream from the promoter start site

Depositor

Richard Murray

Article

McManus et al Arch Biochem Biophys. 2019 Jul 18. pii:

Insert

sfGFP

Tags

Strep tag and TEV site

Expression

Bacterial

Mutation

WT

Promoter

T7

Available Since

Dec. 20, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pY71-tet.I.12

Plasmid

#133533

Purpose

sfGFP driven by a T7 promoter with the tetO sequence 15 bp downstream from the promoter start site

Depositor

Richard Murray

Article

McManus et al Arch Biochem Biophys. 2019 Jul 18. pii:

Insert

sfGFP

Tags

Strep tag and TEV site

Expression

Bacterial

Mutation

WT

Promoter

T7

Available Since

Dec. 20, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pY71-tet.I.11

Plasmid

#133532

Purpose

sfGFP driven by a T7 promoter with the tetO sequence 14 bp downstream from the promoter start site

Depositor

Richard Murray

Article

McManus et al Arch Biochem Biophys. 2019 Jul 18. pii:

Insert

sfGFP

Tags

Strep tag and TEV site

Expression

Bacterial

Mutation

WT

Promoter

T7

Available Since

Dec. 20, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pY71-tet.I.10

Plasmid

#133531

Purpose

sfGFP driven by a T7 promoter with the tetO sequence 13 bp downstream from the promoter start site

Depositor

Richard Murray

Article

McManus et al Arch Biochem Biophys. 2019 Jul 18. pii:

Insert

sfGFP

Tags

Strep tag and TEV site

Expression

Bacterial

Mutation

WT

Promoter

T7

Available Since

Dec. 20, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pY71-tet.I.9

Plasmid

#133530

Purpose

sfGFP driven by a T7 promoter with the tetO sequence 12 bp downstream from the promoter start site

Depositor

Richard Murray

Article

McManus et al Arch Biochem Biophys. 2019 Jul 18. pii:

Insert

sfGFP

Tags

Strep tag and TEV site

Expression

Bacterial

Mutation

WT

Promoter

T7

Available Since

Dec. 20, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pY71-tet.I.7

Plasmid

#133529

Purpose

sfGFP driven by a T7 promoter with the tetO sequence 10 bp downstream from the promoter start site

Depositor

Richard Murray

Article

McManus et al Arch Biochem Biophys. 2019 Jul 18. pii:

Insert

sfGFP

Tags

Strep tag and TEV site

Expression

Bacterial

Mutation

WT

Promoter

T7

Available Since

Dec. 20, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pY71-tet.I.6

Plasmid

#133528

Purpose

sfGFP driven by a T7 promoter with the tetO sequence 9 bp downstream from the promoter start site

Depositor

Richard Murray

Article

McManus et al Arch Biochem Biophys. 2019 Jul 18. pii:

Insert

sfGFP

Tags

Strep tag and TEV site

Expression

Bacterial

Mutation

WT

Promoter

T7

Available Since

Dec. 20, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pY71-tet.I.5

Plasmid

#133527

Purpose

sfGFP driven by a T7 promoter with the tetO sequence 8 bp downstream from the promoter start site

Depositor

Richard Murray

Article

McManus et al Arch Biochem Biophys. 2019 Jul 18. pii:

Insert

sfGFP

Tags

Strep tag and TEV site

Expression

Bacterial

Mutation

WT

Promoter

T7

Available Since

Dec. 20, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pY71-tet.I.3

Plasmid

#133525

Purpose

sfGFP driven by a T7 promoter with the tetO sequence 6 bp downstream from the promoter start site

Depositor

Richard Murray

Article

McManus et al Arch Biochem Biophys. 2019 Jul 18. pii:

Insert

sfGFP

Tags

Strep tag and TEV site

Expression

Bacterial

Mutation

WT

Promoter

T7

Available Since

Dec. 20, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pY71-tet.I.2

Plasmid

#133524

Purpose

sfGFP driven by a T7 promoter with the tetO sequence 5 bp downstream from the promoter start site

Depositor

Richard Murray

Article

McManus et al Arch Biochem Biophys. 2019 Jul 18. pii:

Insert

sfGFP

Tags

Strep tag and TEV site

Expression

Bacterial

Mutation

WT

Promoter

T7

Available Since

Dec. 20, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pY71-tet.I.4

Plasmid

#133526

Purpose

sfGFP driven by a T7 promoter with the tetO sequence 7 bp downstream from the promoter start site

Depositor

Richard Murray

Article

McManus et al Arch Biochem Biophys. 2019 Jul 18. pii:

Insert

sfGFP

Tags

Strep tag and TEV site

Expression

Bacterial

Mutation

WT

Promoter

T7

Available Since

Dec. 12, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pY71-tet.I.1

Plasmid

#133523

Purpose

sfGFP driven by a T7 promoter with the tetO sequence 4 bp downstream from the promoter start site

Depositor

Richard Murray

Article

McManus et al Arch Biochem Biophys. 2019 Jul 18. pii:

Insert

sfGFP

Tags

Strep tag and TEV site

Expression

Bacterial

Mutation

WT

Promoter

T7

Available Since

Dec. 12, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
Ad-MKP-3 C293S

Plasmid

#131525

Purpose

Expresses MKP-3 with C293S mutation in mammalian cells

Depositor

Haiyan Xu

Article

Jiao et al PLoS One. 2012;7(7):e41168. doi: 10.1371

Insert

Mitogen-activated protein kinase phosphatase 3 (Dusp6 Mouse)

Use

Adenoviral

Tags

No

Mutation

changed Cysteine 293 to Serine

Promoter

CMV

Available Since

Oct. 22, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
Ad-FOXO1 1-558

Plasmid

#131531

Purpose

Expresses FOXO1 1-558 in mammalian cells

Depositor

Haiyan Xu

Article

Jiao et al PLoS One. 2012;7(7):e41168. doi: 10.1371

Insert

Forkhead box O1 (Foxo1 Mouse)

Use

Adenoviral

Tags

No

Mutation

only reserved amino acid 1-558

Promoter

CMV

Available Since

Oct. 22, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
Ad-FOXO1 1-456

Plasmid

#131532

Purpose

Expresses FOXO1 1-456 in mammalian cells

Depositor

Haiyan Xu

Article

Jiao et al PLoS One. 2012;7(7):e41168. doi: 10.1371

Insert

Forkhead box O1 (Foxo1 Mouse)

Use

Adenoviral

Tags

No

Mutation

only reserved amino acid 1-456

Promoter

CMV

Available Since

Oct. 22, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
Ad-FOXO1 1-280

Plasmid

#131534

Purpose

Expresses FOXO1 1-280 in mammalian cells

Depositor

Haiyan Xu

Article

Jiao et al PLoS One. 2012;7(7):e41168. doi: 10.1371

Insert

Forkhead box O1 (Foxo1 Mouse)

Use

Adenoviral

Tags

No

Mutation

only reserved amino acid 1-280

Promoter

CMV

Available Since

Oct. 22, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pAcGP67B human Fzd4 CRD (38–160)

Plasmid

#132621

Purpose

expresses human Fzd4 CRD (38-160) in Hi5 insect cell

Depositor

Wenqing Xu

Article

Shen et al Cell Res. 2015 Sep;25(9):1078-81. doi: 1

Insert

Fzd4 CRD (38–160) (FZD4 Human)

Expression

Insect

Available Since

Oct. 17, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pZ_P-ICL1-eGFP

Plasmid

#126716

Purpose

yeast plasmid overexpressing eGFP

Depositor

Menghao Cai

Article

Liu et al Metab Eng. 2019 Jul;54:275-284. doi: 10.

Insert

egfp

Tags

6xHis

Expression

Yeast

Promoter

ICL1

Available Since

Sept. 20, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pZ_P-ACS1-eGFP

Plasmid

#126715

Purpose

yeast plasmid overexpressing eGFP

Depositor

Menghao Cai

Article

Liu et al Metab Eng. 2019 Jul;54:275-284. doi: 10.

Insert

egfp

Expression

Yeast

Promoter

ACS1

Available Since

July 12, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pZ_P-ALD-eGFP

Plasmid

#126714

Purpose

yeast plasmid overexpressing eGFP

Depositor

Menghao Cai

Article

Liu et al Metab Eng. 2019 Jul;54:275-284. doi: 10.

Insert

egfp

Expression

Yeast

Promoter

ALD

Available Since

July 12, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pZ_P-HXT1-eGFP

Plasmid

#126719

Purpose

yeast plasmid overexpressing eGFP

Depositor

Menghao Cai

Article

Liu et al Metab Eng. 2019 Jul;54:275-284. doi: 10.

Insert

egfp

Expression

Yeast

Promoter

HXT1

Available Since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 11921 - 11960 of 23305 results

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