We narrowed to 16,915 results for: EMB

1. pRS426-GFP-Osh1-PH

   Plasmid

   #71372

   Purpose

   For analysis of PH domain localization in yeast

   Depositor

   Mark Lemmon

   Article

   Yu et al Mol Cell. 2004 Mar 12;13(5):677-88.

   Insert

   Osh1-PH
   

   Use

   Tags

   EGFP

   Expression

   Yeast

   Mutation

   Pleckstrin homology (PH) domain; aa267-388

   Promoter

   Available since

   Feb. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

2. pGEX2TK-Skm1-PH

   Plasmid

   #71371

   Purpose

   bacterial expression of PH domain for phosphoinositide binding assay

   Depositor

   Mark Lemmon

   Article

   Yu et al Mol Cell. 2004 Mar 12;13(5):677-88.

   Insert

   Skm1-PH
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Pleckstrin homology (PH) domain; aa1-132

   Promoter

   tac

   Available since

   Feb. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

3. pFUS_B10

   Plasmid

   #31027

   Depositor

   Adam Bogdanove, Daniel Voytas

   Article

   Cermak et al Nucleic Acids Res. 2011 Jul;39(12):e82.

   Insert

   LacZ + BsaI restriction sites
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 30, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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4. iRFP-PAmCherry1-PH_GRP1

   Plasmid

   #92168

   Purpose

   PI(3,4,5)P3 single molecule fluorescent reporter

   Depositor

   Xuelin Lou

   Article

   Ji et al J Biol Chem. 2015 Nov 6;290(45):26978-93

   Insert

   iRFP-PAmCherry1-PHgrp1
   

   Use

   Tags

   iRFP-PAmCherry1

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMaCTag-P31

   Plasmid

   #120042

   Purpose

   Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with V5

   Depositor

   Michael Knop

   Article

   Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

   Insert

   V5
   

   Use

   Pcr template

   Tags

   V5

   Expression

   Mutation

   Promoter

   None

   Available since

   Jan. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMaCTag-12

   Plasmid

   #119991

   Purpose

   Template for C-terminal PCR tagging of mammalian genes (without selection) with mScarlet

   Depositor

   Michael Knop

   Article

   Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

   Insert

   mScarlet
   

   Use

   Pcr template

   Tags

   mScarlet

   Expression

   Mutation

   Promoter

   None

   Available since

   Jan. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMC-XI2

   Plasmid

   #176156

   Purpose

   preassembled yeast MoClo level-2 backbone with GFP dropout for genomic integration in site XI-2

   Depositor

   Florian David

   Article

   Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Feb. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCMB-APr

   Plasmid

   #61183

   Purpose

   Fluorescent reporter for apoplast MtBCP1sp-mCherry expressed under MtBCP1 promoter

   Depositor

   Maria Harrison

   Article

   Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

   Insert

   MtBCPsp-mCherry
   

   Use

   Tags

   mCherry

   Expression

   Plant

   Mutation

   Promoter

   MtBCP1

   Available since

   Feb. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMU-TLEr

   Plasmid

   #61187

   Purpose

   Fluorescent reporter for transitory late endosome mCherry-MtRAB7A2 expressed under AtUBQ10 promoter

   Depositor

   Maria Harrison

   Article

   Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

   Insert

   MtRAB7A2
   

   Use

   Tags

   mCherry

   Expression

   Plant

   Mutation

   Promoter

   AtUBQ10

   Available since

   March 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBB14

    Plasmid

    #18657

    Depositor

    Lynn Enquist

    Article

    Brideau et al J Virol. 1998 Jun . 72(6):4560-70.

    Insert

    Us9-EGFP gene fusion
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    First 106 amino acids of Us9 are intact. The stop…

    Promoter

    Available since

    July 3, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. Tol2_8xCTS-ECFP

    Plasmid

    #200249

    Purpose

    Zebrafish transgenics; Plasmid encodes the sgRNA2_8xCTS-ECFP reporter and could be stably integrated in the zebrafish genome using Tol2 transgenesis

    Depositor

    Tatjana Sauka-Spengler

    Article

    Pelea et al bioRxiv 2023.05.08.539738

    Insert

    8xCTS-ECFP
    

    Use

    Zebrafish expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMB-ACTLr

    Plasmid

    #61194

    Purpose

    Fluorescent reporter for F-actin LifeAct-mCherry expressed under MtBCP1 promoter

    Depositor

    Maria Harrison

    Article

    Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

    Insert

    LifeAct-mCherry
    

    Use

    Tags

    mCherry

    Expression

    Plant

    Mutation

    Promoter

    MtBCP1

    Available since

    March 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMB-MTUBr

    Plasmid

    #61197

    Purpose

    Fluorescent reporter for microtubules mCherry-MAP4-MBD expressed under MtBCP1 promoter

    Depositor

    Maria Harrison

    Article

    Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

    Insert

    MAP-MBD
    

    Use

    Tags

    mCherry

    Expression

    Plant

    Mutation

    Promoter

    MtBCP1

    Available since

    Feb. 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. PsaA

    Plasmid

    #159997

    Purpose

    Expresses Streptococcus pneumoniae PsaA (19-309) in bacterial cells

    Depositor

    Mark Howarth

    Article

    Rahikainen et al Angew Chem Int Ed Engl. 2020 Sep 4. doi:

    Insert

    PsaA (19-309)
    

    Use

    Tags

    His6-tag and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. qTAG-C-miniTurbo-Zeo

    Plasmid

    #207736

    Purpose

    Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a miniTurbo-2A-Zeo cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. qTAG-N-Zeo-miniTurbo

    Plasmid

    #207703

    Purpose

    Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Zeo-2A-miniTurbo cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

17. pOGG216

    Plasmid

    #114002

    Purpose

    Level 2 golden gate cloning vector for medium copy number free-living expression

    Depositor

    Philip Poole

    Article

    Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Domesticated for Golden Gate cloning

    Promoter

    Available since

    Oct. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pACEBac1-GCP3

    Plasmid

    #178069

    Purpose

    Insect cell expression of GCP3

    Depositor

    Tarun Kapoor

    Article

    Wieczorek et al J Cell Biol. 2021 Mar 1;220(3). pii: 211

    Insert

    GCP3 (TUBGCP3 Human)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Jan. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pKL12-GCaMP6f-pHuji bb118

    Plasmid

    #158981

    Purpose

    Constitutive expression (sigma 70 promoter) of GCaMP6f tethered to pHuji fluorophores in E. coli.

    Depositor

    Joel Kralj

    Article

    Bruni et al eLife. 2020 Aug 4;9. pii: 58706. doi: 10

    Insert

    GCaMP6f-pHuji
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    codon optimized for e. coli from Addgene#100833 a…

    Promoter

    Bba_J23118 (sigma 70)

    Available since

    Sept. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pNG1

    Plasmid

    #30985

    Depositor

    Adam Bogdanove, Daniel Voytas

    Article

    Cermak et al Nucleic Acids Res. 2011 Jul;39(12):e82.

    Insert

    repeat NG1
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 30, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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