We narrowed to 18,916 results for: Tec

1. pGWB508

   Plasmid

   #74850

   Purpose

   Gateway cloning compatible binary vector for C-terminal fusion with 6xHis (CaMV35S promoter).

   Depositor

   Tsuyoshi Nakagawa

   Article

   Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

   Type

   Empty backbone

   Expression

   Plant

   Promoter

   CaMV35S

   Available Since

   Oct. 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. EK0294 SFFV-mCherry-SG(s70587-W)SG-EGFP (FLP-IN)

   Plasmid

   #191141

   Purpose

   Positive control for Sensor 1 (EK0285) where the TAG stop codon is replaced with TGG tryptophan codon

   Depositor

   Xiaojing Gao

   Article

   Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

   Insert

   mCherry:s70587-W:EGFP
   

   Expression

   Mammalian

   Mutation

   UAG>UGG (stop>Trp)

   Promoter

   SFFV

   Available Since

   Oct. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMU-4

   Plasmid

   #172353

   Purpose

   CRISPR gene editing in Streptomyces, codon-optimized Cas9 behind theophylline riboswitch and D4 promoter, with custom gRNA

   Depositor

   Eriko Takano

   Article

   Ye et al J Ind Microbiol Biotechnol. 2020 May;47(

   Insert

   D4-TheoR-cas9
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Bacterial

   Promoter

   D4

   Available Since

   Dec. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCH62 (denAsCas12a-KRAB piggyBac)

   Plasmid

   #217333

   Purpose

   piggyBac expression of denAsCas12a-KRAB

   Depositor

   Luke Gilbert

   Article

   Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103

   Insert

   denAsCas12a
   

   Use

   CRISPR

   Tags

   6xMycNLS and HA-SV40NLS-XTEN80-KRAB-P2A-TagBFP2

   Expression

   Mammalian

   Mutation

   D908A/E174R/S542R/K548R

   Promoter

   CAG

   Available Since

   March 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-CaMKII-GRAB_eCB2.0

   Plasmid

   #164608

   Purpose

   Express the endocannabinoid sensor GRAB_eCB2.0 in CaMKII positive neurons

   Depositor

   Yulong Li

   Article

   Dong et al Nat Biotechnol. 2021 Nov 11. pii: 10.103

   Insert

   Endocannabinoid sensor GRAB_eCB2.0
   

   Use

   AAV

   Mutation

   S383T

   Promoter

   CaMKII

   Available Since

   Feb. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBK-YE2-BE3

   Plasmid

   #85176

   Purpose

   Expresses YE2-BE3 in mammalian cells

   Depositor

   David Liu

   Article

   Kim et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

   Insert

   YEE-BE3
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. PDGFR-TMD (MTK 3B) (pZYW025)

   Plasmid

   #194218

   Purpose

   Mammalian Toolkit part 3b encoding the transmembrane domain of PDGFR

   Depositor

   Hana El-Samad

   Article

   Weinberg et al bioRxiv 2021.04.20.440678

   Insert

   PDGFR TMD
   

   Use

   Synthetic Biology

   Promoter

   None

   Available Since

   Sept. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. T2A-mCherry (MTK 4a) (pAN3844)

   Plasmid

   #194223

   Purpose

   Mammalian Toolkit part 4a encoding a T2A polycistronic element followed by mCherry

   Depositor

   Hana El-Samad

   Article

   Weinberg et al bioRxiv 2021.04.20.440678

   Insert

   T2A-mCherry
   

   Use

   Synthetic Biology

   Promoter

   None

   Available Since

   Sept. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. PGK-LCB1_v1.3-NotchGal4VP64-T2A-mCherry (pZYW046)

   Plasmid

   #194229

   Purpose

   Lentiviral expression vector expressing LCB1 SynNotch and mCherry transduction marker

   Depositor

   Hana El-Samad

   Article

   Weinberg et al bioRxiv 2021.04.20.440678

   Insert

   PGK-LCB1-SynNotch-T2A-mCherry
   

   Use

   Lentiviral

   Promoter

   PGK

   Available Since

   Sept. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. TALEN1257

    Plasmid

    #32280

    Depositor

    Keith Joung

    Article

    Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

    Insert

    TAL array targeting hey2 (hey2 Zebrafish)
    

    Use

    T7

    Tags

    3X FLAG and WT FOKI

    Expression

    Mammalian

    Available Since

    Oct. 27, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGS1222

    Plasmid

    #63791

    Purpose

    Vector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal eGFP Twin-Strep tag followed by URA3 selection marker

    Depositor

    Monika Golas, Bjoern Sander

    Article

    Rai et al Mol Biotechnol. 2014 Jun 27.

    Insert

    eGFP-Twin-Strep tag followed by URA3
    

    Use

    E. coli cloning vector

    Tags

    eGFP-Twin-Strep tag

    Promoter

    none

    Available Since

    April 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSL2621 (pCas8_Vch)

    Plasmid

    #200893

    Purpose

    Expresses human codon-optimized V. cholerae Cas8 from a CMV promoter.

    Depositor

    Samuel H. Sternberg

    Article

    Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

    Insert

    VchCas8
    

    Tags

    NLS

    Expression

    Mammalian

    Available Since

    May 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSL2623 (pCas6_Vch)

    Plasmid

    #200895

    Purpose

    Expresses human codon-optimized V. cholerae Cas6 from a CMV promoter.

    Depositor

    Samuel H. Sternberg

    Article

    Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

    Insert

    VchCas6
    

    Tags

    NLS

    Expression

    Mammalian

    Available Since

    May 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAVS1-TLR6

    Plasmid

    #118997

    Purpose

    Targeting vector for human AAVS1 with TLR6 reporter insert

    Depositor

    Ralf Kuehn

    Article

    Bashir et al BMC Biotechnol. 2020 Oct 23;20(1):57. do

    Insert

    AAVS1 (PPP1R12C, AAVS1 Human)
    

    Expression

    Mammalian

    Available Since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTn7·X-nia

    Plasmid

    #122592

    Purpose

    Plasmid for genomic integraction of xylS-Pm->nia into the Tn7 insertion site

    Depositor

    Pablo Ivan Nikel

    Article

    Volke et al Microb Biotechnol. 2019 Mar 12. doi: 10.

    Insert

    xylS/Pm->Nia
    

    Use

    Synthetic Biology; Tn7 genomic integration

    Expression

    Bacterial

    Promoter

    Pm

    Available Since

    March 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAGK112

    Plasmid

    #228127

    Purpose

    Express node 1531 retron with split retron ncRNA and gRNA to edit (SNIP + PAM recode) HEK4 gene in human cells

    Depositor

    Seth Shipman

    Article

    Khan et al Nat Biotechnol. 2024 Sep 17. doi: 10.103

    Insert

    Retron node 1531 split retron ncRNA and gRNA to edit HEK4 gene (SNP + PAM recode) in human cells
    

    Expression

    Mammalian

    Promoter

    CAG for RT, U6/H1 for ncRNA/gRNA

    Available Since

    Dec. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. shCDK4/6(2)

    Plasmid

    #73555

    Purpose

    vector encoding both shRNA against CDK4(2) and shRNA against CDK6(2)

    Depositor

    Gerardo Ferbeyre

    Article

    Acevedo et al Cancer Res. 2016 Jun 1;76(11):3252-64. d

    Insert

    shRNA against CDK4 + shRNA against CDK6
    

    Use

    Retroviral

    Expression

    Mammalian

    Promoter

    LTR

    Available Since

    Aug. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

18. pTS1051-Tier1-PhCMV-4xCox8

    Plasmid

    #169490

    Purpose

    Tier-1 vector encoding PhCMV-driven four-fold repeat of a mitochondrial matrix localization signal derived from mitochondrial matrix protein Cox8 (PhCMV-4xCox8-pA)

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PCMV-driven mitochondrial localization signal from cytochrome c oxidase subunit 8
    

    Expression

    Mammalian

    Promoter

    PhCMV

    Available Since

    June 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pShoHELIX-sgRNA_entry (BO3)

    Plasmid

    #181784

    Purpose

    Expresses ShoHELIX containing a nicking I-AniI fusion to ShoTnsB. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).

    Depositor

    Benjamin Kleinstiver

    Article

    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    nAniI-ShoTnsB, ShoTnsC, ShoTniQ, ShoCas12k
    

    Expression

    Bacterial

    Mutation

    nAniI = K227M, F80K, L232K

    Promoter

    Lac and J23119

    Available Since

    March 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-ABE-CT

    Plasmid

    #112876

    Purpose

    AAV inverted terminal repeat based vector plasmid encoding the C-terminal half of nCas9

    Depositor

    Jin-Soo Kim

    Article

    Ryu et al Nat Biotechnol. 2018 Jul;36(6):536-539.

    Insert

    ABE-CT
    

    Use

    AAV

    Available Since

    July 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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