We narrowed to 446 results for: DHFR
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Plasmid#116058PurposeReporter plasmid for measuring protease activityDepositorInsertCit-tehc-DHFR
ExpressionMammalianAvailable SinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJB02
Plasmid#111959PurposeExpresses GST-GFP-DHFR; construct generated for pull-down and localization experimentsDepositorInsertsmEGFP
eDHFR
TagsDHFRExpressionBacterialMutationeDHFR;residues 2-159 and enhanced GFP with monome…PromoterT7Available SinceAug. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
DHFR-GFP-th-GluA1
Plasmid#173008PurposeFor light regulated release of GluA1 from the endoplasmic reticulum. Driven by synapsin promoter. Thrombin site is included between GFP and GluA1.DepositorInsertDHFR-GFP-th-GluA1
UseLentiviralTagsGFPExpressionMammalianPromoterhuman synapsinAvailable SinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-tvD-Cit-tevs-DHFR
Plasmid#116054PurposeReporter plasmid for measuring protease activityDepositorInserttvD-Cit-tevs-DHFR
ExpressionMammalianAvailable SinceOct. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
UPR pBARUra3
Plasmid#45354DepositorInsertDHFR Ub Rek Ura3
ExpressionYeastPromoterpCUP1Available SinceJune 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
PB-PGK-tvD-Cit-tevs-DHFR
Plasmid#116053PurposeReporter plasmid for measuring protease activityDepositorInserttvD-Cit-tevs-DHFR
ExpressionMammalianAvailable SinceOct. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
PB-PGK-ZhcD-Cit-tevs-DHFR
Plasmid#116051PurposeReporter plasmid for measuring protease activityDepositorInsertZhcD-Cit-tevs-DHFR
ExpressionMammalianAvailable SinceOct. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
PB-PGK-teD-Cit-hcvs-DHFR
Plasmid#116050PurposeReporter plasmid for measuring protease activityDepositorInsertteD-Cit-hcvs-DHFR
ExpressionMammalianAvailable SinceOct. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
Calb1-T2A-dgCre targeting vector
Plasmid#114438PurposeTarget dgCre into the endogenous mouse Calb1 locus at the stop codonDepositorInsertdgCre
UseMouse TargetingTagsDHFR domain and EGFPPromoternone; utilizes endogenous Calb1 promoter for expr…Available SinceJan. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
WT_S1-H1
Plasmid#218392Purpose3-exon minigene, with SRSF1 motif-rich middle exon, and hnRNPA1 motif-rich region in second intronDepositorInsertModified CHO DHFR minigene
ExpressionMammalianPromotertetracycline-responsive promoterAvailable SinceJune 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
MUT_S1-H1
Plasmid#218394Purpose3-exon minigene, with SRSF1 motif-rich middle exon, hnRNPA1 motif-rich region in second intron, and AG>GG mutation in final 3' splice siteDepositorInsertModified CHO DHFR minigene
ExpressionMammalianPromotertetracycline-responsive promoterAvailable SinceMay 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTETDuet_M42FG121VDHFR_R166QTS
Plasmid#191298PurposeBacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)DepositorInsertfolA, thyA
ExpressionBacterialMutationM42F/G121V DHFR, R166Q TYMSPromoterT7 (folA), Tet (thyA)Available SinceJune 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTETDuet_WTDHFR_WTTS
Plasmid#191282PurposeBacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)DepositorInsertfolA, thyA
ExpressionBacterialMutationWT DHFR, WT TYMSPromoterT7 (folA), Tet (thyA)Available SinceJune 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBS-SKII-URA-GAL1-DHFR-3xHA
Plasmid#188934PurposeIntegration cassette to create N-terminal fusions for fluorescent taggingDepositorInsertURA-GAL1-DHFR-3xHA
ExpressionYeastAvailable SinceSept. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJB03
Plasmid#111961PurposeExpresses MBP-GFP-DHFR; construct generated for pull-down and localization experimentsDepositorInsertsmEGFP
eDHFR
TagsDHFRExpressionBacterialMutationeDHFR;residues 2-159 and enhanced GFP with monome…PromoterTacAvailable SinceAug. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFloxed DHFR-TS*
Plasmid#100605PurposepUC19 with loxP-pDHFR-TS:DHFR-TS*(Pyr-R)-Stop-loxP-DHFR-TS 3' UTRDepositorInsertFloxed TgDHFR-TS*
UseToxoplasma expressionMutationPyrimethamine resistanceAvailable SinceSept. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
mCherry-eDHFR
Plasmid#107266PurposemCherry-eDHFR in pIVT vector for in vitro transcriptionDepositorAvailable SinceMarch 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSuccessor
Plasmid#228391Purposeintended to be co-transformed along with pDawn-Ag43 to generate successor strain (SS-GFP) for multipattern biofilm lithographyDepositorInsertpConst23100-sfGFP__pLambda-DHFR
UseSynthetic BiologyAvailable SinceNov. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
WT_H1-S1
Plasmid#218400Purpose3-exon minigene, with SRSF1 motif-rich middle exon, and hnRNPA1 motif-rich region in first intronDepositorInsertModified CHO DHFR minigene
ExpressionMammalianPromotertetracycline-responsive promoterAvailable SinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
MUT_S1-spacer-H1
Plasmid#218399Purpose3-exon minigene, with SRSF1 motif-rich middle exon, hnRNPA1 motif-rich region in second intron following a neutral spacer, and AG>GG mutation in final 3' splice siteDepositorInsertModified CHO DHFR minigene
ExpressionMammalianPromotertetracycline-responsive promoterAvailable SinceJune 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
MUT_S1-N
Plasmid#218398Purpose3-exon minigene, with SRSF1 motif-rich middle exon, neutral region in second intron, and AG>GG mutation in final 3' splice siteDepositorInsertModified CHO DHFR minigene
ExpressionMammalianPromotertetracycline-responsive promoterAvailable SinceMay 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
MUT_N-H1
Plasmid#218397Purpose3-exon minigene, with neutral middle exon, hnRNPA1 motif-rich region in second intron, and AG>GG mutation in final 3' splice siteDepositorInsertModified CHO DHFR minigene
ExpressionMammalianPromotertetracycline-responsive promoterAvailable SinceMay 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
MUT_S7-H1
Plasmid#218396Purpose3-exon minigene, with SRSF7 motif-rich middle exon, hnRNPA1 motif-rich region in second intron, and AG>GG mutation in final 3' splice siteDepositorInsertModified CHO DHFR minigene
ExpressionMammalianPromotertetracycline-responsive promoterAvailable SinceMay 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
MUT_S1-H1,NR
Plasmid#218395Purpose3-exon minigene, with SRSF1 motif-rich middle exon, hnRNPA1 motif-rich region in second intron, both randomly mutated to avoid sequence repeats, and AG>GG mutation in final 3' splice siteDepositorInsertModified CHO DHFR minigene
ExpressionMammalianPromotertetracycline-responsive promoterAvailable SinceMay 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
WT_S7-H1
Plasmid#218393Purpose3-exon minigene, with SRSF7 motif-rich middle exon, and hnRNPA1 motif-rich region in second intronDepositorInsertModified CHO DHFR minigene
ExpressionMammalianPromotertetracycline-responsive promoterAvailable SinceMay 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTETDuet_F31YDHFR_R127ATS
Plasmid#191312PurposeBacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)DepositorInsertfolA, thyA
ExpressionBacterialMutationF31Y DHFR/R127A TYMSPromoterT7 (folA), Tet (thyA)Available SinceOct. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTETDuet_D27NDHFR_Q33STS
Plasmid#191289PurposeBacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)DepositorInsertfolA, thyA
ExpressionBacterialMutationD27N DHFR, Q33S TYMSPromoterT7 (folA), Tet (thyA)Available SinceJune 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTETDuet_M42FG121VDHFR_Q33STS
Plasmid#191292PurposeBacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)DepositorInsertfolA, thyA
ExpressionBacterialMutationM42F/G121V DHFR, Q33S TYMSPromoterT7 (folA), Tet (thyA)Available SinceJune 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTETDuet_F31YDHFR_R166QTS
Plasmid#191311PurposeBacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)DepositorInsertfolA, thyA
ExpressionBacterialMutationF31Y DHFR/R166Q TYMSPromoterT7 (folA), Tet (thyA)Available SinceJune 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTETDuet_F31YDHFR_WTTS
Plasmid#191310PurposeBacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)DepositorInsertfolA, thyA
ExpressionBacterialMutationF31Y DHFR/WT TYMSPromoterT7 (folA), Tet (thyA)Available SinceJune 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTETDuet_F31YDHFR_Q33STS
Plasmid#191313PurposeBacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)DepositorInsertfolA, thyA
ExpressionBacterialMutationF31Y DHFR/Q33S TYMSPromoterT7 (folA), Tet (thyA)Available SinceJune 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTETDuet_F31YG121VDHFR_Q33STS
Plasmid#191293PurposeBacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)DepositorInsertfolA, thyA
ExpressionBacterialMutationF31Y/G121V DHFR, Q33S TYMSPromoterT7 (folA), Tet (thyA)Available SinceJune 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTETDuet_WTDHFR_R166QTS
Plasmid#191294PurposeBacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)DepositorInsertfolA, thyA
ExpressionBacterialMutationWT DHFR, R166Q TYMSPromoterT7 (folA), Tet (thyA)Available SinceJune 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTETDuet_D27NDHFR_R166QTS
Plasmid#191295PurposeBacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)DepositorInsertfolA, thyA
ExpressionBacterialMutationD27N DHFR, R166Q TYMSPromoterT7 (folA), Tet (thyA)Available SinceJune 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTETDuet_M42FDHFR_R166QTS
Plasmid#191296PurposeBacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)DepositorInsertfolA, thyA
ExpressionBacterialMutationM42F DHFR, R166Q TYMSPromoterT7 (folA), Tet (thyA)Available SinceJune 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTETDuet_G121VDHFR_R166QTS
Plasmid#191297PurposeBacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)DepositorInsertfolA, thyA
ExpressionBacterialMutationG121V DHFR, R166Q TYMSPromoterT7 (folA), Tet (thyA)Available SinceJune 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTETDuet_F31YG121VDHFR_R166QTS
Plasmid#191299PurposeBacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)DepositorInsertfolA, thyA
ExpressionBacterialMutationF31Y/G121V DHFR, R166Q TYMSPromoterT7 (folA), Tet (thyA)Available SinceJune 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTETDuet_WTDHFR_R127ATS
Plasmid#191300PurposeBacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)DepositorInsertfolA, thyA
ExpressionBacterialMutationWT DHFR, R127A TYMSPromoterT7 (folA), Tet (thyA)Available SinceJune 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTETDuet_D27NDHFR_R127ATS
Plasmid#191301PurposeBacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)DepositorInsertfolA, thyA
ExpressionBacterialMutationD27N DHFR, R127A TYMSPromoterT7 (folA), Tet (thyA)Available SinceJune 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTETDuet_M42FDHFR_R127ATS
Plasmid#191302PurposeBacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)DepositorInsertfolA, thyA
ExpressionBacterialMutationM42F DHFR, R127A TYMSPromoterT7 (folA), Tet (thyA)Available SinceJune 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTETDuet_G121VDHFR_R127ATS
Plasmid#191303PurposeBacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)DepositorInsertfolA, thyA
ExpressionBacterialMutationG121V DHFR, R127A TYMSPromoterT7 (folA), Tet (thyA)Available SinceJune 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTETDuet_F31YG121VDHFR_R127ATS
Plasmid#191304PurposeBacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)DepositorInsertfolA, thyA
ExpressionBacterialMutationF31Y/G121V DHFR, R127A TYMSPromoterT7 (folA), Tet (thyA)Available SinceJune 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTETDuet_M42FG121VDHFR_R127ATS
Plasmid#191305PurposeBacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)DepositorInsertfolA, thyA
ExpressionBacterialMutationM42F/G121V DHFR, R127A TYMSPromoterT7 (folA), Tet (thyA)Available SinceJune 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTETDuet_D27NDHFR_WTTS
Plasmid#191283PurposeBacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)DepositorInsertfolA, thyA
ExpressionBacterialMutationD27N DHFR, WT TYMSPromoterT7 (folA), Tet (thyA)Available SinceJune 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTETDuet_M42FDHFR_WTTS
Plasmid#191284PurposeBacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)DepositorInsertfolA, thyA
ExpressionBacterialMutationM42F DHFR, WT TYMSPromoterT7 (folA), Tet (thyA)Available SinceJune 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTETDuet_G121VDHFR_WTTS
Plasmid#191285PurposeBacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)DepositorInsertfolA, thyA
ExpressionBacterialMutationG121V DHFR, WT TYMSPromoterT7 (folA), Tet (thyA)Available SinceJune 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTETDuet_M42FG121VDHFR_WTTS
Plasmid#191286PurposeBacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)DepositorInsertfolA, thyA
ExpressionBacterialMutationM42F/G121V DHFR, WT TYMSPromoterT7 (folA), Tet (thyA)Available SinceJune 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTETDuet_F31YG121VDHFR_WTTS
Plasmid#191287PurposeBacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)DepositorInsertfolA, thyA
ExpressionBacterialMutationF31Y/G121V DHFR, WT TYMSPromoterT7 (folA), Tet (thyA)Available SinceJune 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTETDuet_WTDHFR_Q33STS
Plasmid#191288PurposeBacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)DepositorInsertfolA, thyA
ExpressionBacterialMutationWT DHFR, Q33S TYMSPromoterT7 (folA), Tet (thyA)Available SinceJune 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTETDuet_M42FDHFR_Q33STS
Plasmid#191290PurposeBacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)DepositorInsertfolA, thyA
ExpressionBacterialMutationM42F DHFR, Q33S TYMSPromoterT7 (folA), Tet (thyA)Available SinceJune 5, 2023AvailabilityAcademic Institutions and Nonprofits only