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LT1_86_TU6of6_HOhomology

Plasmid

#194425

Purpose

TU6 backbone (for TU #6 for plasmid with 6 TUs) with HO homology arms for Saccharomyces cerevisiae

Depositor

Lisbeth Olsson

Article

Torello Pianale et al ACS Synth Biol. 2023 Aug 8. doi: 10.1021

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

NA

Promoter

Available since

May 8, 2023

Availability

Academic Institutions and Nonprofits only

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LT1_63_TU1of2-6_X2homology

Plasmid

#194405

Purpose

TU1 backbone (for TU #1 for plasmid with 2-6 TUs) with X2 homology arms for Saccharomyces cerevisiae

Depositor

Lisbeth Olsson

Article

Torello Pianale et al ACS Synth Biol. 2023 Aug 8. doi: 10.1021

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

NA

Promoter

Available since

May 8, 2023

Availability

Academic Institutions and Nonprofits only

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LT1_64_TU2of2_X2homology

Plasmid

#194406

Purpose

TU2 backbone (for TU #2 for plasmid with 2 TUs) with X2 homology arms for Saccharomyces cerevisiae

Depositor

Lisbeth Olsson

Article

Torello Pianale et al ACS Synth Biol. 2023 Aug 8. doi: 10.1021

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

NA

Promoter

Available since

May 8, 2023

Availability

Academic Institutions and Nonprofits only

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LT1_65_TU2of3-6_X2homology

Plasmid

#194407

Purpose

TU2 backbone (for TU #2 for plasmid with 3-6 TUs) with X2 homology arms for Saccharomyces cerevisiae

Depositor

Lisbeth Olsson

Article

Torello Pianale et al ACS Synth Biol. 2023 Aug 8. doi: 10.1021

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

NA

Promoter

Available since

May 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
LT1_66_TU3of3_X2homology

Plasmid

#194408

Purpose

TU3 backbone (for TU #3 for plasmid with 3 TUs) with X2 homology arms for Saccharomyces cerevisiae

Depositor

Lisbeth Olsson

Article

Torello Pianale et al ACS Synth Biol. 2023 Aug 8. doi: 10.1021

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

NA

Promoter

Available since

May 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
LT1_67_TU3of4-6_X2homology

Plasmid

#194409

Purpose

TU3 backbone (for TU #3 for plasmid with 4-6 TUs) with X2 homology arms for Saccharomyces cerevisiae

Depositor

Lisbeth Olsson

Article

Torello Pianale et al ACS Synth Biol. 2023 Aug 8. doi: 10.1021

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

NA

Promoter

Available since

May 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
LT1_68_TU4of4_X2homology

Plasmid

#194410

Purpose

TU4 backbone (for TU #4 for plasmid with 4 TUs) with X2 homology arms for Saccharomyces cerevisiae

Depositor

Lisbeth Olsson

Article

Torello Pianale et al ACS Synth Biol. 2023 Aug 8. doi: 10.1021

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

NA

Promoter

Available since

May 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
LT1_69_TU4of5-6_X2homology

Plasmid

#194411

Purpose

TU4 backbone (for TU #4 for plasmid with 5-6 TUs) with X2 homology arms for Saccharomyces cerevisiae

Depositor

Lisbeth Olsson

Article

Torello Pianale et al ACS Synth Biol. 2023 Aug 8. doi: 10.1021

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

NA

Promoter

Available since

May 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
LT1_70_TU5of5_X2homology

Plasmid

#194412

Purpose

TU5 backbone (for TU #5 for plasmid with 5 TUs) with X2 homology arms for Saccharomyces cerevisiae

Depositor

Lisbeth Olsson

Article

Torello Pianale et al ACS Synth Biol. 2023 Aug 8. doi: 10.1021

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

NA

Promoter

Available since

May 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
LT1_71_TU5of6_X2homology

Plasmid

#194413

Purpose

TU5 backbone (for TU #5 for plasmid with 6 TUs) with X2 homology arms for Saccharomyces cerevisiae

Depositor

Lisbeth Olsson

Article

Torello Pianale et al ACS Synth Biol. 2023 Aug 8. doi: 10.1021

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

NA

Promoter

Available since

May 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
YN2_1_LT58_X2site

Plasmid

#177705

Purpose

Expresses Cas9 and sgRNA cassette for targeting site X2 in yeast Saccharomyces cerevisiae

Depositor

Lisbeth Olsson

Article

Torello Pianale et al Front Microbiol. 2022 Jan 7;12:802169. d

Insert

Cas9

Use

CRISPR

Tags

Expression

Yeast

Mutation

Promoter

Available since

Jan. 13, 2022

Availability

Academic Institutions and Nonprofits only

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LT1_36_QUEEN-2m

Plasmid

#177708

Purpose

Plasmid for genome integration in X2 site expressing QUEEN-2m (ATP biosensor)

Depositor

Lisbeth Olsson

Article

Torello Pianale et al Front Microbiol. 2022 Jan 7;12:802169. d

Insert

pTEF1-QUEEN2m

Use

Used as donor dna in genome integration after lin…

Tags

Expression

Yeast

Mutation

Promoter

Available since

Jan. 13, 2022

Availability

Academic Institutions and Nonprofits only

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LT1_33_pTEFmut8-mCherry

Plasmid

#177706

Purpose

Construct for genome integration expressing constitutively mCherry under pTEFmut8 promoter

Depositor

Lisbeth Olsson

Article

Torello Pianale et al Front Microbiol. 2022 Jan 7;12:802169. d

Insert

pTEFmut8-mCherry

Use

Used as donor dna in genome integration after lin…

Tags

Expression

Yeast

Mutation

Promoter

Available since

Jan. 13, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
LT1_34_sfpHluorin

Plasmid

#177707

Purpose

Plasmid for genome integration in X2 site expressing sfpHluorin (intracellular pH biosensor)

Depositor

Lisbeth Olsson

Article

Torello Pianale et al Front Microbiol. 2022 Jan 7;12:802169. d

Insert

pTEF1-sfpHluorin

Use

Used as donor in genome integration after lineari…

Tags

Expression

Yeast

Mutation

Promoter

Available since

Jan. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-iRFP

Plasmid

#64887

Purpose

Marking retinal neurons with infrared fluorescent proteins (iRFP) to preserve photoresponsiveness

Depositor

Tomomi Ichinose

Article

Fyk-Kolodziej et al Biotechniques. 2014 Nov 1;57(5):245-53.

Insert

iRFP

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 23, 2015

Availability

Academic Institutions and Nonprofits only

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YN2_1_IL50_HOlocus

Plasmid

#194426

Purpose

Expresses Cas9 and sgRNA cassettes for targeting the HO locus in yeast Saccharomyces cerevisiae

Depositor

Lisbeth Olsson

Article

Torello Pianale et al ACS Synth Biol. 2023 Aug 8. doi: 10.1021

Insert

Cas9

Use

Tags

Expression

Yeast

Mutation

NA

Promoter

Available since

May 8, 2023

Availability

Academic Institutions and Nonprofits only

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YN2_1_LT84_RPL13A

Plasmid

#177712

Purpose

Expresses Cas9 and sgRNA cassette for targeting RPL13A in yeast Saccharomyces cerevisiae

Depositor

Lisbeth Olsson

Article

Torello Pianale et al Front Microbiol. 2022 Jan 7;12:802169. d

Insert

Cas9

Use

CRISPR

Tags

Expression

Yeast

Mutation

Promoter

Available since

Jan. 13, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
LT2_15_UPRpro_X2site

Plasmid

#194427

Purpose

Plasmid for genome integration in X2 site expressing UPRpro - Sensor for Unfolded Protein Response

Depositor

Lisbeth Olsson

Article

Torello Pianale et al ACS Synth Biol. 2023 Aug 8. doi: 10.1021

Insert

UPRpro

Use

Used as donor dna in genome integration after lin…

Tags

Expression

Yeast

Mutation

NA

Promoter

Available since

May 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
LT2_33_PyruPro_X2site

Plasmid

#194429

Purpose

Plasmid for genome integration in X2 site expressing PyruPro (Pyruvate consumption Probe)

Depositor

Lisbeth Olsson

Article

Torello Pianale et al ACS Synth Biol. 2023 Aug 8. doi: 10.1021

Insert

PyruPro

Use

Used as donor dna in genome integration after lin…

Tags

Expression

Yeast

Mutation

NA

Promoter

Available since

May 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
LT2_34_EthPro_X2site

Plasmid

#194430

Purpose

Plasmid for genome integration in X2 site expressing ResPro (Ethanol consumption Probe)

Depositor

Lisbeth Olsson

Article

Torello Pianale et al ACS Synth Biol. 2023 Aug 8. doi: 10.1021

Insert

EthPro

Use

Used as donor dna in genome integration after lin…

Tags

Expression

Yeast

Mutation

NA

Promoter

Available since

May 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge