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mcherry-DN KASH

Plasmid

#125553

Purpose

mcherry labeled DN-KASH

Depositor

Daniel Conway

Article

Mayer et al Cell Mol Bioeng. 2019 Jul 11;12(4):289-3

Insert

SYNE1 (SYNE1 Human)

Use

Tags

mcherry

Expression

Mammalian

Mutation

Truncated form: only the KASH domain of nesprin-…

Promoter

CMV

Available since

July 25, 2019

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPRv1_UQCRC2

Plasmid

#177982

Purpose

lentiviral vector expressing Cas9 and a sgRNA targeting UQCRC2

Depositor

David Sabatini, Jessica Spinelli

Article

Spinelli et al Science. 2021 Dec 3;374(6572):1227-1237.

Insert

sgRNA targeting UQCRC2 (UQCRC2 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

N/A

Promoter

U6

Available since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA-Dest47- WTN23C11orf83-GFP

Plasmid

#65845

Purpose

Mammalian expression of the wild type N terminal part (N23, transmembrane part of 23 aa) of C11orf83/UQCC3 fused to GFP protein (C-ter)

Depositor

Amos Bairoch

Article

Desmurs et al Mol Cell Biol. 2015 Apr;35(7):1139-56. d

Insert

Transmembrane (23 AA) of UQCC3 (UQCC3 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

WT Transmembrane

Promoter

Available since

July 7, 2015

Availability

Academic Institutions and Nonprofits only

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GST-Delta23C11orf83

Plasmid

#65849

Purpose

bacterial expression of GST (N-ter) - Delta 23 C11orf83/UQCC3 (UQCC3 protein depleted of its N-terminal transmembrane domain)

Depositor

Amos Bairoch

Article

Desmurs et al Mol Cell Biol. 2015 Apr;35(7):1139-56. d

Insert

UQCC3 depleted of its N terminal transmembrane part (UQCC3 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

deletion of the 23 first aa (TM)

Promoter

Available since

July 7, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2-R5A-K6A C11orf83-V5

Plasmid

#65850

Purpose

Mammalian expression of C terminally V5-tagged C11orf83/UQCC3 : MUTANT R5A-K6A

Depositor

Amos Bairoch

Article

Desmurs et al Mol Cell Biol. 2015 Apr;35(7):1139-56. d

Insert

Mutated UQCC3 (UQCC3 Human)

Use

Tags

V5

Expression

Mammalian

Mutation

R5A-K6A

Promoter

Available since

July 7, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2-R5D-K6D C11orf83-V5

Plasmid

#65851

Purpose

Mammalian expression of C terminally V5-tagged C11orf83/UQCC3 : MUTANT R5D-K6D

Depositor

Amos Bairoch

Article

Desmurs et al Mol Cell Biol. 2015 Apr;35(7):1139-56. d

Insert

Mutated UQCC3 (UQCC3 Human)

Use

Tags

V5

Expression

Mammalian

Mutation

R5D-K6D

Promoter

Available since

July 7, 2015

Availability

Academic Institutions and Nonprofits only

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pinducer 20 DN-KASH

Plasmid

#125554

Purpose

Tet-ON inducible lentivirus expressing mcherry-labeled DN KASH

Depositor

Daniel Conway

Article

Mayer et al Cell Mol Bioeng. 2019 Jul 11;12(4):289-3

Insert

SYNE1 (SYNE1 Human)

Use

Lentiviral

Tags

mcherry

Expression

Mutation

Truncated form: only the KASH domain of nesprin-…

Promoter

tetracycline responsive element

Available since

July 25, 2019

Availability

Academic Institutions and Nonprofits only

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pCW57.1 tTA UQCRC2

Plasmid

#177978

Purpose

All-in-one dox-repressible expression of cDNA

Depositor

David Sabatini, Jessica Spinelli

Article

Spinelli et al Science. 2021 Dec 3;374(6572):1227-1237.

Insert

UQCRC2 (UQCRC2 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Silent point mutations to UQCRC2 sgRNA target

Promoter

Available since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pET-Duet1-6xHis-TEV-OPTN

Plasmid

#190192

Purpose

Plasmid for the expression of 6xHis-TEV-OPTN. Internal reference: SMC397

Depositor

Sascha Martens

Article

Zaffagnini et al EMBO J. 2018 Mar 1;37(5). pii: embj.2017

Insert

optineurin (OPTN Human)

Use

Tags

6xHis and TEV

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA-Dest47- R5A-K5A N23 C11orf83-GFP

Plasmid

#65846

Purpose

Mammalian expression of the mutated N terminal part (R5A-K5A N23, transmembrane part of 23 aa) of C11orf83/UQCC3 fused to GFP protein (C-ter)

Depositor

Amos Bairoch

Article

Desmurs et al Mol Cell Biol. 2015 Apr;35(7):1139-56. d

Insert

Transmembrane (23 AA) of UQCC3 (UQCC3 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

R5A-K6A

Promoter

Available since

July 7, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA-Dest47- R5D-K6D N23 C11orf83-GFP

Plasmid

#65847

Purpose

Mammalian expression of the mutated N terminal part (R5D-K5D N23, transmembrane part of 23 aa) of C11orf83/UQCC3 fused to GFP protein (C-ter)

Depositor

Amos Bairoch

Article

Desmurs et al Mol Cell Biol. 2015 Apr;35(7):1139-56. d

Insert

Transmembrane (23 AA) of UQCC3 (UQCC3 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

R5D-K6D

Promoter

Available since

July 7, 2015

Availability

Academic Institutions and Nonprofits only

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UQCRB_pLX307

Plasmid

#98381

Purpose

Lentiviral expression of UQCRB

Depositor

Sefi Rosenbluh, William Hahn

Article

Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

Insert

UQCRB (UQCRB Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

E1Fa

Available since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pinducer 20 DN-KASHΔPPPL

Plasmid

#129280

Purpose

Tet-ON inducible lentivirus expressing mcherry-labeled DN KASHΔPPPL

Depositor

Daniel Conway

Article

Zhang et al Curr Biol. 2019 Aug 2. pii: S0960-9822(1

Insert

SYNE1 (SYNE1 Human)

Use

Lentiviral

Tags

mcherry

Expression

Mutation

the last 4 amino acids (PPPL) of the KASH domain …

Promoter

CMV

Available since

Aug. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pET-Duet1_6xHis-TEV-eGFP-P62

Plasmid

#190929

Purpose

Plasmid for the expression and purification of GFP labelled p62. Internal reference: SMC390

Depositor

Sascha Martens

Article

Turco et al Nat Commun. 2021 Sep 1;12(1):5212. doi:

Insert

P62 (SQSTM1 Human)

Use

Tags

EGFP

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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