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Nano-lantern(Ca2+)_300/pcDNA3

Plasmid

#51983

Purpose

mammalian expression of a Nano-lantern-based luminescent Ca2+ indicator with Kd=300nM

Depositor

Takeharu Nagai

Article

Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

Insert

Nano-lantern_CaM_E104Q-3GS

Use

Tags

Expression

Mammalian

Mutation

S257G in Rluc8

Promoter

Available since

April 15, 2014

Availability

Academic Institutions and Nonprofits only

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OeNL(Ca2+)_18μ-pcDNA3

Plasmid

#111928

Purpose

Orange color bright luminescent calcium indicator with intermediate affinity

Depositor

Takeharu Nagai

Article

Hossain et al ACS Chem Biol. 2018 Mar 9. doi: 10.1021/

Insert

OeNL(Ca2+)_18μ

Use

Tags

Expression

Mammalian

Mutation

E67D, E104D, D133E at CaM

Promoter

CMV

Available since

June 26, 2018

Availability

Academic Institutions and Nonprofits only

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pET15b+PCNA1 P186C

Plasmid

#66173

Purpose

Expresses the P186C mutant of Sulfolobus solfataricus PCNA1

Depositor

Teruyuki Nagamune

Article

Hirakawa et al Biotechnol Bioeng. 2013 Jul;110(7):1858-

Insert

DNA polymerase sliding clamp 1

Use

Tags

His6 tag

Expression

Bacterial

Mutation

P186C

Promoter

T7 promoter

Available since

June 29, 2015

Availability

Academic Institutions and Nonprofits only

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pASKt15C+SrtA5

Plasmid

#64985

Purpose

Expresses the P94R/D160N/D165A/K190E/K196T mutant of Staphylococcus aureus sortase A in E. coli

Depositor

Teruyuki Nagamune

Article

Hirakawa et al Biotechnol J. 2015 Apr 10. doi: 10.1002/

Insert

sortase A

Use

Tags

His6 tag and S tag

Expression

Bacterial

Mutation

deleted amino acids 1-59, P94R, D160N, D165A, K19…

Promoter

tetracycline promoter/operator

Available since

June 9, 2015

Availability

Academic Institutions and Nonprofits only

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CeNL(Ca2+)_19μ-pcDNA3

Plasmid

#111927

Purpose

Cyan color bright luminescent calcium indicator with intermediate affinity

Depositor

Takeharu Nagai

Article

Hossain et al ACS Chem Biol. 2018 Mar 9. doi: 10.1021/

Insert

CeNL(Ca2+)_19μ

Use

Tags

Expression

Mammalian

Mutation

E67D, F92W, E104D, D133E at CaM

Promoter

CMV

Available since

June 26, 2018

Availability

Academic Institutions and Nonprofits only

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Nano-lantern(Ca2+)_600/pcDNA3

Plasmid

#51982

Purpose

mammalian expression of a Nano-lantern-based luminescent Ca2+ indicator with Kd=600nM

Depositor

Takeharu Nagai

Article

Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

Insert

Nano-lantern_CaM_E104Q-2G

Use

Tags

Expression

Mammalian

Mutation

S257G in Rluc8

Promoter

Available since

April 15, 2014

Availability

Academic Institutions and Nonprofits only
pASKt15C+SrtA7woH

Plasmid

#65020

Purpose

Expresses the P94R/E105K/E108A/D160N/D165K/K190E/K196T mutant of Staphylococcus aureus sortase A in E. coli

Depositor

Teruyuki Nagamune

Article

Hirakawa et al Biotechnol J. 2015 Apr 10. doi: 10.1002/

Insert

sortase A

Use

Tags

S tag

Expression

Bacterial

Mutation

deleted amino acids 1-59, P94R, E105K, E108A, D16…

Promoter

tetracycline promoter/operator

Available since

June 9, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
Nano-lantern(Ca2+)_117/pcDNA3

Plasmid

#51985

Purpose

mammalian expression of a Nano-lantern-based luminescent Ca2+ indicator with Kd=117nM

Depositor

Takeharu Nagai

Article

Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

Insert

Nano-lantern_CaM-2GS

Use

Tags

Expression

Mammalian

Mutation

S257G in Rluc8

Promoter

Available since

April 15, 2014

Availability

Academic Institutions and Nonprofits only
Nano-lantern(Ca2+)_600/pRSETB

Plasmid

#51976

Purpose

bacterial expression of a Nano-lantern-based luminescent Ca2+ indicator with Kd=600nM

Depositor

Takeharu Nagai

Article

Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

Insert

Nano-lantern_CaM_E104Q-2G

Use

Tags

6xHis

Expression

Bacterial

Mutation

S257G in Rluc8

Promoter

Available since

March 24, 2014

Availability

Academic Institutions and Nonprofits only
pGK415-phlTA2-1E

Plasmid

#165970

Purpose

Expresses phlTA(2-1E) mutant

Depositor

Jun Ishii

Article

Tominaga et al Nat Commun. 2021 Mar 23;12(1):1846. doi:

Insert

phlF

Use

Synthetic Biology

Tags

Nuclear localization signal and three copies of V…

Expression

Yeast

Mutation

P5S, S6P, K86T, F109L, Q117R, E143K, codon-optimi…

Promoter

pPGK1 mutant

Available since

March 29, 2021

Availability

Academic Institutions and Nonprofits only

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