Addgene

We narrowed to 25,494 results for: STI

Showing: 871 - 900 of 25494 results

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EJ2GFP-puro

Plasmid

#44025

Depositor

Jeremy Stark

Article

Bennardo et al PLoS Genet. 2008 Jun 27;4(6):e1000110. d

Insert

EJ2GFP egfp-based chromosomal break reporter

Use

Tags

Expression

Mammalian

Mutation

insertion of I-SceI, 3 frame stop, and flanking m…

Promoter

pCAGGS

Available since

May 3, 2013

Availability

Academic Institutions and Nonprofits only

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S96 ELP

Plasmid

#68393

Purpose

Elastin-like polypeptide (VPGxG) with 96 repeats of Serine guest residue

Depositor

Andrew MacKay

Article

Shah et al Protein Sci. 2012 Jun;21(6):743-50. doi:

Insert

S96 ELP

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 1, 2015

Availability

Academic Institutions and Nonprofits only

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AiP14801 - pAAV-AiE0390m_3xC2-minBG-iCre(R297T)-BGHpA (Alias: CN4801)

Plasmid

#208163

Purpose

AiE0390m_3xC2 is an optimized enhancer sequence designed to drive AAV-mediated Cre recombination in brain astrocytes

Depositor

Jonathan Ting, The Allen Institute for Brain Science

Insert

iCreR297T

Use

AAV

Tags

Expression

Mutation

NA

Promoter

Beta Globin minimal promoter

Available since

April 10, 2024

Availability

Academic Institutions and Nonprofits only

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FusX DdCBE DddA6 1397N

Plasmid

#221934

Purpose

Backbone plasmid for FusX-based assembly of DdCBE arm with DddA6 1397N

Depositor

Stephen Ekker

Article

Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

Type

Empty backbone

Use

Synthetic Biology

Tags

3xFLAG

Expression

Mutation

DddA: Q1310R, S1330I, T1380I

Promoter

Available since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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FusX DdCBE DddA11 1397N

Plasmid

#221935

Purpose

Backbone plasmid for FusX-based assembly of DdCBE arm with DddA11 1397N

Depositor

Stephen Ekker

Article

Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

Type

Empty backbone

Use

Synthetic Biology

Tags

3xFLAG

Expression

Mutation

DddA: S1330I, A1341V, N1342S, E1370K, T1380I

Promoter

Available since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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FusX alphaDdCBE DddA6-11 1397C

Plasmid

#221938

Purpose

Backbone plasmid for FusX-based assembly of alphaDdCBE arm with DddA6/11 1397C

Depositor

Stephen Ekker

Article

Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

Type

Empty backbone

Use

Synthetic Biology

Tags

3xFLAG

Expression

Mutation

TALE: Q231R, W232G, S233A | DddA: T1413I

Promoter

Available since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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AiP13318 - pAAV-AiE0390m_3xC2-minBG-SYFP2-4x2C-WPRE3-BGHpA (Alias: CN3318)

Plasmid

#208167

Purpose

AiE0390m_3xC2 is an optimized enhancer sequence designed to drive AAV-mediated transgene expression in brain astrocytes

Depositor

Jonathan Ting, The Allen Institute for Brain Science

Insert

SYFP2

Use

AAV

Tags

Expression

Mutation

NA

Promoter

Beta Globin minimal promoter

Available since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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PhdeA-cfp

Plasmid

#127940

Purpose

CFP fluorescent reporter for HdeA

Depositor

Mary Dunlop

Article

Rossi et al Commun Biol. 2019 Jul 11;2:259. doi: 10.

Insert

PhdeA-cfp

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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AiP13999 - pAAV-AiE0387m-minBG-iCre(R297T)-BGHpA (Alias: CN3999)

Plasmid

#208166

Purpose

AiE0387m is an enhancer sequence designed to drive AAV-mediated Cre recombination in brain astrocytes

Depositor

Jonathan Ting, The Allen Institute for Brain Science

Insert

iCreR297T

Use

AAV

Tags

Expression

Mutation

NA

Promoter

Beta Globin minimal promoter

Available since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pJL1121

Plasmid

#78188

Purpose

intestinal GFP expression by fasting in worms

Depositor

Junho Lee

Article

Jo et al Cell Metab. 2009 May;9(5):440-8. doi: 10

Insert

fil-2 (fil-2 Nematode)

Use

Tags

Expression

Worm

Mutation

Promoter

fil-2

Available since

May 20, 2016

Availability

Academic Institutions and Nonprofits only
AiP13998 - pAAV-AiE0390m-minBG-iCre(R297T)-BGHpA (Alias: CN3998)

Plasmid

#208162

Purpose

AiE0390m is an enhancer sequence designed to drive AAV-mediated Cre recombination in brain astrocytes

Depositor

Jonathan Ting, The Allen Institute for Brain Science

Insert

iCreR297T

Use

AAV

Tags

Expression

Mutation

NA

Promoter

Beta Globin minimal promoter

Available since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pID3.01B

Plasmid

#22720

Depositor

Geraldine Seydoux

Article

D'Agostino et al Dev Biol. 2006 Apr 1. 292(1):244-52.

Insert

unc-119 rescuing fragment / pie-1promoter-GFP- gateway destination cassette B -pie-1 3’

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 21, 2010

Availability

Academic Institutions and Nonprofits only

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PpurA-cfp

Plasmid

#127936

Purpose

CFP fluorescent reporter for PurA

Depositor

Mary Dunlop

Article

Rossi et al Commun Biol. 2019 Jul 11;2:259. doi: 10.

Insert

PpurA-cfp

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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PsodA-cfp

Plasmid

#127945

Purpose

CFP fluorescent reporter for SodA

Depositor

Mary Dunlop

Article

Rossi et al Commun Biol. 2019 Jul 11;2:259. doi: 10.

Insert

PsodA-cfp

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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PacrAB-cfp

Plasmid

#127944

Purpose

CFP fluorescent reporter for AcrAB

Depositor

Mary Dunlop

Article

Rossi et al Commun Biol. 2019 Jul 11;2:259. doi: 10.

Insert

PacrAB-cfp

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
P_70-cfp

Plasmid

#127942

Purpose

CFP fluorescent reporter for _70

Depositor

Mary Dunlop

Article

Rossi et al Commun Biol. 2019 Jul 11;2:259. doi: 10.

Insert

P_70-cfp

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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PinaA-cfp

Plasmid

#127937

Purpose

CFP fluorescent reporter for InaA

Depositor

Mary Dunlop

Article

Rossi et al Commun Biol. 2019 Jul 11;2:259. doi: 10.

Insert

PinaA-cfp

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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C113S-HA

Plasmid

#113062

Purpose

Expresses worm KVS-1 C113S in mammalian cells

Depositor

Federico Sesti

Article

Sesti et al Trends Cell Biol. 2010 Jan;20(1):45-51.

Insert

C113S-KVS-1 (kvs-1 Nematode)

Use

Tags

HA

Expression

Mammalian

Mutation

C113S

Promoter

Available since

Aug. 23, 2018

Availability

Academic Institutions and Nonprofits only

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pimEJ5GFP

Plasmid

#44026

Depositor

Jeremy Stark

Article

Bennardo et al PLoS Genet. 2008 Jun 27;4(6):e1000110. d

Insert

EJ5GFP egfp-based chromosomal break reporter

Use

Tags

Expression

Mammalian

Mutation

pCAGGS promoter and eGFP separated by pgkPURO cas…

Promoter

pCAGGS

Available since

May 3, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pHBFD1Bad

Plasmid

#53564

Purpose

Produces an active HBFD (carotenoid 4-hydroxy-β-ring 4-dehydrogenase) enzyme in E. coli when expression is induced with arabinose

Depositor

Francis X Cunningham Jr

Article

Cunningham FX et al Plant Cell. 2011 Aug;23(8):3055-69. doi:

Insert

hbfd1 cDNA

Use

Tags

Expression

Bacterial

Mutation

Promoter

araBAD

Available since

Jan. 21, 2015

Availability

Academic Institutions and Nonprofits only

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p274 EF1a_EGFP_hH11

Plasmid

#164851

Purpose

EGFP-expression vector for human H11 locus

Depositor

Philip Jordan

Article

Pryzhkova et al Tissue Eng Regen Med. 2020 Apr;17(2):223

Insert

EGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

EF1-alpha

Available since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
V48 ELP

Plasmid

#68395

Purpose

Elastin-like polypeptide (VPGxG) with 48 repeats of valine guest residue

Depositor

Andrew MacKay

Article

Shah et al Protein Sci. 2012 Jun;21(6):743-50. doi:

Insert

V48 ELP

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 1, 2015

Availability

Academic Institutions and Nonprofits only

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pBSV2B

Plasmid

#118228

Purpose

Empty E. coli - B. burgdorferi shuttle vector, blasticidin S resistant

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2018 Oct 12. pii

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 17, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
FusX alphaDdCBE DddA6 1397N

Plasmid

#221939

Purpose

Backbone plasmid for FusX-based assembly of alphaDdCBE arm with DddA6 1397N

Depositor

Stephen Ekker

Article

Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

Type

Empty backbone

Use

Synthetic Biology

Tags

3xFLAG

Expression

Mutation

TALE: Q231R, W232G, S233A | DddA: Q1310R, S1330I,…

Promoter

Available since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
FusX alphaDdCBE DddA11 1397N

Plasmid

#221940

Purpose

Backbone plasmid for FusX-based assembly of alphaDdCBE arm with DddA11 1397N

Depositor

Stephen Ekker

Article

Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

Type

Empty backbone

Use

Synthetic Biology

Tags

3xFLAG

Expression

Mutation

TALE: Q231R, W232G, S233A | DddA: S1330I, A1341V,…

Promoter

Available since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCBFD1/HBFD1Bad

Plasmid

#53364

Purpose

Will convert beta-carotene into astaxanthin in E. coli when expression of the hbfd1 cDNA is induced with arabinose. Use with pAC-BETA or pAC-BETAipi.

Depositor

Francis X Cunningham Jr

Article

Cunningham FX et al Plant Cell. 2011 Aug;23(8):3055-69. doi:

Insert

cbfd1

Use

Tags

Expression

Bacterial

Mutation

Promoter

lac

Available since

Jan. 21, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
p273 EF1a_mCherry2_hH11

Plasmid

#164852

Purpose

mCherry2-expression vector for human H11 locus

Depositor

Philip Jordan

Article

Pryzhkova et al Tissue Eng Regen Med. 2020 Apr;17(2):223

Insert

mCherry2

Use

Tags

Expression

Mammalian

Mutation

Promoter

EF1-alpha

Available since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pCRISPRi-D2

Plasmid

#182927

Purpose

inducible CRISPRi plasmid with gRNA targeting to psbD gene in Synechocystis 6803

Depositor

Himadri Pakrasi

Article

Liu et al ACS Synth Biol. 2020 Jun 19;9(6):1441-14

Insert

ddcpf1

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

April 6, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
p663-UBC-miniTurbo-V5-PIP5K1A_IDG-K

Plasmid

#214462

Purpose

Gateway destination clone of PIP5K1A (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

Depositor

Ben Major

Insert

miniTurbo-V5-PIP5K1A (PIP5K1A Human)

Use

Lentiviral; Gateway destination

Tags

miniTurbo-V5

Expression

Mammalian

Mutation

Promoter

Ubiquitin

Available since

March 13, 2024

Availability

Academic Institutions and Nonprofits only

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p8.9 NdeltaSB

Plasmid

#132929

Purpose

Minimal HIV-1 packaging plasmid for gag and pol expression

Depositor

Jeremy Luban

Article

Berthoux et al J Virol. 2004 Nov;78(21):11739-50. doi:

Insert

gag and pol

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Aug. 24, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge