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TCT-Universal plasmid

Plasmid

#221852

Purpose

A universal plasmid for expressing any protein with TetraCysteine motif

Depositor

Artur Krężel

Article

Antonenko et al ACS Omega. 2024 Oct 30;9(45):45127-45137

Insert

Tetra Cysteine tag

Expression

Bacterial

Mutation

No

Available Since

Aug. 29, 2024

Availability

Academic Institutions and Nonprofits only

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eSrtA-Δ24-ASN

Plasmid

#221849

Purpose

Expresses delta 24 alpha synuclein as 6XHis-SUMO-eSrtA-LPNTG-Δ24-ASN fusion protein

Depositor

Artur Krężel

Article

Singh et al Microb Cell Fact. 2024 Nov 30;23(1):325.

Insert

alpha-synuclein (SNCA Human)

Expression

Bacterial

Mutation

No

Available Since

Aug. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pET28-Butelase

Plasmid

#221848

Purpose

Expresses asparaginyl endopeptidase butelase

Depositor

Artur Krężel

Article

Singh et al Microb Cell Fact. 2024 Nov 30;23(1):325.

Insert

Butelase 1 ligase

Expression

Bacterial

Mutation

No

Available Since

Aug. 29, 2024

Availability

Academic Institutions and Nonprofits only

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Ub-LPNTG-pET

Plasmid

#221850

Purpose

Expresses Ub with sortase motif and His tag

Depositor

Artur Krężel

Article

Antonenko et al ACS Omega. 2024 Oct 30;9(45):45127-45137

Insert

Ubiquitin

Expression

Bacterial

Mutation

Arg74ProLeuThr

Available Since

Aug. 29, 2024

Availability

Academic Institutions and Nonprofits only

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SUMO-LPQTG-pET

Plasmid

#221851

Purpose

Expresses SUMO-2 with sortase motif and His tag

Depositor

Artur Krężel

Article

Antonenko et al ACS Omega. 2024 Oct 30;9(45):45127-45137

Insert

SUMO-2 (SUMO2 Human)

Expression

Bacterial

Mutation

Gln88Leu, Gln89Pro

Available Since

Aug. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pMRXIP-GFP-TAPP1(PHD)

Plasmid

#221757

Purpose

Stable expression of GFP-TAPP1(PHD) in mammalian cells

Depositor

Noboru Mizushima

Article

Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

Insert

PLEKHA1 (PLEKHA1 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

tandem TAPP1 (PHD) (amino acids 184-304)

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pMRXIP-Btk1(PHD)-GFP

Plasmid

#221756

Purpose

Stable expression of Btk1(PHD)-GFP in mammalian cells

Depositor

Noboru Mizushima

Article

Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

Insert

Btk (BTK Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

amino acids 1–177

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pMRXIP-GFP-PKD C1ab

Plasmid

#221755

Purpose

Stable expression of GFP-PKD C1ab in mammalian cells

Depositor

Noboru Mizushima

Article

Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

Insert

Prkd1(138-343) (Prkd1 Mouse)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

amino acids 138-343

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pMRXIP-GFP-PLCd1(PHD)

Plasmid

#221753

Purpose

Stable expression of GFP-PLCd1(PHD) in mammalian cells

Depositor

Noboru Mizushima

Article

Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

Insert

PLCd1 (PLCD1 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

amino acids 1–175

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pMRXIP-GFP-2xFYVE

Plasmid

#221750

Purpose

Stable expression of GFP-2xFYVE in mammalian cells

Depositor

Noboru Mizushima

Article

Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

Insert

tandem HRS FYVE domain

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

two FYVE domains (amino acids 147-223), linked by…

Available Since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pMRXIP-GFP-0K9Q

Plasmid

#221743

Purpose

Stable expression of GFP-0K9Q in mammalian cells to monitor membrane charge

Depositor

Noboru Mizushima

Article

Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

Insert

C -terminal region of KRAS (KRAS Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

deleted amino acids 1-170, mutated K to Q

Available Since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pMRXIP-GFP-1K8Q

Plasmid

#221742

Purpose

Stable expression of GFP-1K8Q in mammalian cells to monitor membrane charge

Depositor

Noboru Mizushima

Article

Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

Insert

C -terminal region of KRAS (KRAS Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

deleted amino acids 1-170, mutated K to Q

Available Since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pMRXIP-GFP-3K6Q

Plasmid

#221741

Purpose

Stable expression of GFP-3K6Q in mammalian cells to monitor membrane charge

Depositor

Noboru Mizushima

Article

Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

Insert

C -terminal region of KRAS (KRAS Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

deleted amino acids 1-170, mutated K to Q

Available Since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pMRXIP-GFP-5K4Q

Plasmid

#221740

Purpose

Stable expression of GFP-5K4Q in mammalian cells to monitor membrane charge

Depositor

Noboru Mizushima

Article

Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

Insert

C -terminal region of KRAS (KRAS Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

deleted amino acids 1-170, mutated K to Q

Available Since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV4-HA-p31(comet)-S102A

Plasmid

#221698

Purpose

Mammalian expression of HA-tagged human p31(comet) (S102A)

Depositor

Derrick Sek Tong Ong

Article

Chuah et al Cell Death Differ. 2023 Aug;30(8):1973-1

Insert

MAD2BP1

Expression

Mammalian

Mutation

S102A

Available Since

Aug. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV4-HA-p31(comet)

Plasmid

#221697

Purpose

Mammalian expression of HA-tagged human p31(comet)

Depositor

Derrick Sek Tong Ong

Article

Chuah et al Cell Death Differ. 2023 Aug;30(8):1973-1

Insert

MAD2BP1

Expression

Mammalian

Available Since

Aug. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pHAGE-RNF8-R42A

Plasmid

#221702

Purpose

Lentiviral expression of human RNF8 (R42A) in mammalian cells

Depositor

Derrick Sek Tong Ong

Article

Chuah et al Cell Death Differ. 2023 Aug;30(8):1973-1

Insert

RNF8 (RNF8 Human)

Use

Lentiviral

Mutation

R42A

Available Since

Aug. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pHAGE-RNF8-C403S

Plasmid

#221703

Purpose

Lentiviral expression of human RNF8 (C403S) in mammalian cells

Depositor

Derrick Sek Tong Ong

Article

Chuah et al Cell Death Differ. 2023 Aug;30(8):1973-1

Insert

RNF8 (RNF8 Human)

Use

Lentiviral

Mutation

C403S

Available Since

Aug. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pHAGE-RNF8-S157A

Plasmid

#221704

Purpose

Lentiviral expression of human RNF8 (S157A) in mammalian cells

Depositor

Derrick Sek Tong Ong

Article

Chuah et al Cell Death Differ. 2023 Aug;30(8):1973-1

Insert

RNF8 (RNF8 Human)

Use

Lentiviral

Mutation

S157A

Available Since

Aug. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV4-HA-MAD2

Plasmid

#221696

Purpose

Mammalian expression of HA-tagged human MAD2

Depositor

Derrick Sek Tong Ong

Article

Chuah et al Cell Death Differ. 2023 Aug;30(8):1973-1

Insert

MAD2

Expression

Mammalian

Available Since

Aug. 19, 2024

Availability

Academic Institutions and Nonprofits only

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