We narrowed to 1,051 results for: SEF

1. shRNA_NUDC_2

   Plasmid

   #215211

   Purpose

   Supression of shNUDC_2 expression

   Depositor

   Sefi Rosenbluh

   Article

   Liu et al Nat Cell Biol. 2024 Aug;26(8):1359-1372.

   Insert

   NUDC shRNA 2 (NUDC Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. shRNA_NUDC_1

   Plasmid

   #215212

   Purpose

   Supression of shNUDC_1 expression

   Depositor

   Sefi Rosenbluh

   Article

   Liu et al Nat Cell Biol. 2024 Aug;26(8):1359-1372.

   Insert

   NUDC shRNA 1 (NUDC Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. shRNA_MTO1_2

   Plasmid

   #215213

   Purpose

   Supression of shMTO1_2 expression

   Depositor

   Sefi Rosenbluh

   Article

   Liu et al Nat Cell Biol. 2024 Aug;26(8):1359-1372.

   Insert

   MTO1 shRNA 2 (MTO1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. SAM-DNMT3A empty

   Plasmid

   #213164

   Purpose

   Empty vector (no sgRNA) for induction of global DNA methylation.

   Depositor

   Sefi Rosenbluh

   Article

   Hosseinpour et al Nat Commun. 2024 Dec 1;15(1):10449. doi:

   Type

   Empty backbone

   Use

   CRISPR, Lentiviral, and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   E1Fa and U6

   Available since

   Dec. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. SAM-DNMT3A+sgGFP

   Plasmid

   #213165

   Purpose

   Vector with sgGFP for induction of global DNA methylation.

   Depositor

   Sefi Rosenbluh

   Article

   Hosseinpour et al Nat Commun. 2024 Dec 1;15(1):10449. doi:

   Insert

   sgGFP
   

   Use

   CRISPR, Lentiviral, and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   E1Fa and U6 and PGK

   Available since

   Dec. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. SAM-DNMT3A+sgAAVS1_145

   Plasmid

   #213166

   Purpose

   Vector with sgAAVS1 for induction of global DNA methylation.

   Depositor

   Sefi Rosenbluh

   Article

   Hosseinpour et al Nat Commun. 2024 Dec 1;15(1):10449. doi:

   Insert

   sgAAVS1_145
   

   Use

   CRISPR, Lentiviral, and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   E1Fa and U6

   Available since

   Dec. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. SAM-DNMT3A+sgAAVS1_118

   Plasmid

   #213167

   Purpose

   Vector with sgAAVS1 for induction of global DNA methylation.

   Depositor

   Sefi Rosenbluh

   Article

   Hosseinpour et al Nat Commun. 2024 Dec 1;15(1):10449. doi:

   Insert

   sgAAVS1_118
   

   Use

   CRISPR, Lentiviral, and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   E1Fa and U6

   Available since

   Dec. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. SAM-DNMT3A-inactiveEmpty

   Plasmid

   #213168

   Purpose

   Empty Vector (no sgRNA) used as a negative control (inactive DNMT3A) for induction of global DNA methylation.

   Depositor

   Sefi Rosenbluh

   Article

   Hosseinpour et al Nat Commun. 2024 Dec 1;15(1):10449. doi:

   Type

   Empty backbone

   Use

   CRISPR, Lentiviral, and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   E1Fa and U6

   Available since

   Dec. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. GFP-Miro1 (1-177 fused to 562-618)

   Plasmid

   #127627

   Purpose

   First GTPase domain of human Miro1 fused to C-terminus

   Depositor

   Josef Kittler

   Article

   Covill-Cooke et al EMBO Rep. 2020 Feb 5;21(2):e49865. doi:

   Insert

   Miro1 (RHOT1 Human)
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   1-177 fused to 562-618

   Promoter

   Available since

   Oct. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. GFP-Miro1 (412-618)

    Plasmid

    #127628

    Purpose

    GTPase2 domain of human Miro1 including transmembrane domain and C-terminal amino acids

    Depositor

    Josef Kittler

    Article

    Covill-Cooke et al EMBO Rep. 2020 Feb 5;21(2):e49865. doi:

    Insert

    Miro1 (RHOT1 Human)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    412-618 only

    Promoter

    Available since

    Oct. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. p-EF1a-CreERT2-3Xflag-T2A-eBFP2

    Plasmid

    #170186

    Purpose

    This Cre-ERT2 expressing construct can be used to inducibly recombine loxp sites. It can be used with poly-loxP containing plasmids to generate timestamp barcodes useful for linage tracing

    Depositor

    Bradley Bernstein

    Article

    Kim et al Cell Rep. 2020 Oct 6;33(1):108222. doi:

    Insert

    CreERT2-T2A-eBFP2
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1 alpha

    Available since

    July 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. APOBEC3G_pLX307

    Plasmid

    #98345

    Purpose

    Lentiviral expression of APOBEC3G

    Depositor

    Sefi Rosenbluh, William Hahn

    Article

    Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

    Insert

    APOBEC3G (APOBEC3G Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    E1Fa

    Available since

    Aug. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCDH-cGAS-E225A D227A-mCherry2-EF1-Puro

    Plasmid

    #132771

    Purpose

    Interacts with cytoplasmic DNA. Useful for identifying sites of nuclear envelope rupture.

    Depositor

    Jan Lammerding

    Article

    Denais et al Science. 2016 Apr 15;352(6283):353-8. do

    Insert

    Cyclic GMP-AMP synthase (CGAS Human)
    

    Use

    Lentiviral

    Tags

    mCherry2

    Expression

    Mammalian

    Mutation

    E225A D227A

    Promoter

    Available since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. RBM10_pLX307

    Plasmid

    #98371

    Purpose

    Lentiviral expression of RBM10

    Depositor

    Sefi Rosenbluh, William Hahn

    Article

    Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

    Insert

    RBM10 (RBM10 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Isoform 2

    Promoter

    E1Fa

    Available since

    Aug. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. SLC25A1_pLX307

    Plasmid

    #98374

    Purpose

    Lentiviral expression of SLC25A1

    Depositor

    Sefi Rosenbluh, William Hahn

    Article

    Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

    Insert

    SLC25A1 (SLC25A1 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    E1Fa

    Available since

    Aug. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pKK-TEV-CyOFP1

    Plasmid

    #105799

    Purpose

    Expression of your protein of interest in fusion with orange fluorescent protein at the C-terminus (cleavable by TEV) (PMID: 27240196). CyOFP1 is useful for multi-channel imaging.

    Depositor

    Andrzej Dziembowski

    Article

    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

    Type

    Empty backbone

    Use

    Flp-in competent

    Tags

    TEV-CyOFP1

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pVITRO1-M80-F2-IgG1/κ

    Plasmid

    #50383

    Purpose

    Expresses human IgG1/κ antibody isotype with unknown specificity. Useful vector for cloning of human antibodies with kappa light chain

    Depositor

    Andrew Beavil

    Article

    Dodev et al Sci Rep. 2014 Jul 30;4:5885. doi: 10.103

    Inserts

    human Gamma 1 heavy chain expression cassette with unknown specificity
    human Kappa light chain expression cassette with unknown specificity
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Mouse Elongation Factor 1 Alpha and Rat Elongatio…

    Available since

    Jan. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRK5-HA-Ubiquitin-K11R

    Plasmid

    #121154

    Purpose

    Mammalian expression of HA tagged ubiquitin K11R

    Depositor

    Josef Kittler

    Article

    Birsa et al J Biol Chem. 2014 May 23;289(21):14569-8

    Insert

    Ubiquitin C (UBC Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    K11 mutated to an R

    Promoter

    Available since

    Feb. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRK5-HA-Ubiquitin-K6

    Plasmid

    #121151

    Purpose

    Mammalian expression of HA tagged ubiquitin with K6 only, other lysines mutated to arginines

    Depositor

    Josef Kittler

    Article

    Birsa et al J Biol Chem. 2014 May 23;289(21):14569-8

    Insert

    Ubiquitin C (UBC Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    All Ks apart from K6 mutated to Rs

    Promoter

    Available since

    Feb. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRK5-HA-Ubiquitin-K27R

    Plasmid

    #121155

    Purpose

    Mammalian expression of HA tagged ubiquitin K27R

    Depositor

    Josef Kittler

    Article

    Birsa et al J Biol Chem. 2014 May 23;289(21):14569-8

    Insert

    Ubiquitin C (UBC Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    K27 mutated to an R

    Promoter

    Available since

    Feb. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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