We narrowed to 159,238 results for: addgene

1. pAAV-hSyn-fDIO-rM3D(Gs)-mCherry-WPREpA

   Plasmid

   #154869

   Purpose

   Human synapsin-1 promoter; Flp-dependent rM3D(Gs) DREADD-mCherry

   Depositor

   Ulrik Gether

   Insert

   rM3D(Gs)
   

   Use

   AAV; Flp-frt

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Human Synapsin-1

   Available since

   Aug. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. RAB3B (3DZ8)

   Plasmid

   #32879

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   RAB3B (RAB3B Human)
   

   Use

   Tags

   His tag (with TEV cleavage)

   Expression

   Bacterial

   Mutation

   amino acids 18-190 only

   Promoter

   Available since

   Jan. 11, 2012

   Availability

   Industry, Academic Institutions, and Nonprofits

3. 6CVZ (RFWD3)

   Plasmid

   #119964

   Purpose

   Baculovirus expression for structure determination

   Depositor

   Cheryl Arrowsmith

   Insert

   RFWD3 (RFWD3 Human)
   

   Use

   Baculovirus expression

   Tags

   6xHis-TEV

   Expression

   Mutation

   Promoter

   Polyhedrin

   Available since

   Jan. 8, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pEN_TT CXADR-V5

   Plasmid

   #220358

   Purpose

   Gateway entry vector for an inducible V5-tagged CXADR

   Depositor

   Kevin Janes

   Insert

   CXADR (CXADR Human)
   

   Use

   Entry vector for gateway cloning

   Tags

   V5

   Expression

   Mutation

   Promoter

   Available since

   Aug. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. 7TF

   Plasmid

   #24306

   Depositor

   Roel Nusse

   Article

   Fuerer et al PLoS One. 2010 . 5(2):e9370.

   Insert

   7xTcf-FFluc
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 5, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMTF1.1.0-gDNA

   Plasmid

   #113797

   Purpose

   CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor MTF1

   Depositor

   Kevin White

   Insert

   MTF1 (MTF1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. NUDT4 (NUDT4A-c001)

   Plasmid

   #98241

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   NUDT4A (NUDT4 Human)
   

   Use

   Tags

   His-6-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. UBH14

   Plasmid

   #28158

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   E3 ligase UHRF2 (UHRF2 Human)
   

   Use

   Tags

   His tag with thrombin cleavage

   Expression

   Bacterial

   Mutation

   SRA Domain (residues 419-648)

   Promoter

   Available since

   Feb. 28, 2011

   Availability

   Industry, Academic Institutions, and Nonprofits

9. pSpCas9_mouse_Adar1_ccB

   Plasmid

   #158122

   Purpose

   deletion mAdar1

   Depositor

   Emanuela Felley-Bosco

   Insert

   Adar1 (Adar Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSpCas9_mouse_Adar1_ccA

    Plasmid

    #158120

    Purpose

    deletion mAdar1

    Depositor

    Emanuela Felley-Bosco

    Insert

    Adar1 (Adar Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAIO-Ef1a-PE2-GFP:KCNQ2-C201R

    Plasmid

    #185060

    Purpose

    Ef1a driven PE2 plasmid with pegRNA for editing C201R mutation in KCNQ2 gene. See Addgene plasmid #184445

    Depositor

    Bobby Koeleman

    Insert

    U6:pegRNA:scaffold:PBS+RT template
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Nov. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pZC03 (pFA6a-TEV-6xGly-V5-His3MX)

    Plasmid

    #44072

    Depositor

    Tim Formosa

    Type

    Empty backbone

    Use

    Pcr-based yeast c-terminal tagging

    Tags

    6xGly linker, ADH1 transcription terminator, His3…

    Expression

    Mutation

    Promoter

    Available since

    April 2, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-CMV-FLEX-SaCas9-U6-sgSlc6a3

    Plasmid

    #159902

    Purpose

    Mutagenesis of Slc6a3

    Depositor

    Larry Zweifel

    Insert

    Slc6a3 (Slc6a3 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. p-dCas9-dTET1-H1672Y-D1674A-Hygro

    Plasmid

    #104413

    Purpose

    transient expression of dCas9-dTET1 fusion protein

    Depositor

    Stephan Beck

    Insert

    dTET1
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    H1672Y-D1674A

    Promoter

    CMV promoter

    Available since

    April 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTF264 (pFA6a-TEV-6xGly-3xFlag-His3MX)

    Plasmid

    #44071

    Depositor

    Tim Formosa

    Type

    Empty backbone

    Use

    Pcr-based yeast c-terminal tagging

    Tags

    3xFlag, 6xGly linker, ADH1 transcription terminat…

    Expression

    Mutation

    Promoter

    Available since

    April 2, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. USP08 (3N3K)

    Plasmid

    #28115

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    USP08 (USP8 Human)
    

    Use

    Tags

    His tag with TEV site

    Expression

    Bacterial

    Mutation

    Catalytic domain only.

    Promoter

    Available since

    Feb. 28, 2011

    Availability

    Industry, Academic Institutions, and Nonprofits

17. pTF270 (pFA6a-PSc-6xGly-3xFlag-KanMX)

    Plasmid

    #44089

    Depositor

    Tim Formosa

    Type

    Empty backbone

    Use

    Pcr-based yeast c-terminal tagging

    Tags

    3xFlag, 6xGly linker, ADH1 transcription terminat…

    Expression

    Mutation

    Promoter

    Available since

    April 2, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. pKM343

    Plasmid

    #69762

    Purpose

    Retroviral expression of CENP-I

    Depositor

    Iain Cheeseman

    Insert

    CENP-I (CENPI Human)
    

    Use

    Retroviral

    Tags

    eGFP-TEV-S

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

19. SETD3

    Plasmid

    #32873

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    SETD3 (SETD3 Human)
    

    Use

    Tags

    His tag (with TEV cleavage)

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 6, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

20. pSL1423

    Plasmid

    #194904

    Purpose

    pSLI-TGD/TurboID, C-terminal tag of recodonized TurboID for Plasmodium falciparum

    Depositor

    Scott Lindner

    Insert

    TurboID
    

    Use

    Plasmodium falciparum

    Tags

    GFPmut2

    Expression

    Mutation

    Promoter

    None. This allows a C-terminal fusion to a protei…

    Available since

    Feb. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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