Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

Filter By

We narrowed to 6,933 results for: CAD

Showing: 5981 - 6000 of 6933 results

  3. pAAV-TRE-flex-ChR2 (E123T/T159C)-EYFP

    Plasmid
    #135176
    Purpose
    AAV expression of TRE(TRE3G)-driven, Cre-dependent, humanized channelrhodopsin E123T/T159C mutant fused to EYFP for optogenetic activation.
    Depositor
    Tetsuo Yamamori
    Article
    Ebina et al Proc Natl Acad Sci U S A. 2019 Nov 5;116
    Inserts
    hChR2
    TRE3G
    Use
    AAV and Cre/Lox
    Tags
    EYFP (C terminal on insert)
    Expression
    Mammalian
    Mutation
    E123T and T159C
    Promoter
    TRE3G
    Available since
    Jan. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    135176-plasmid-map-sequence-id-268585

    Enlarge

  6. mCherry-CRY2-INPP5E(D556A)

    Plasmid
    #79563
    Purpose
    expression of mCherry-tagged CRY2PHR fused to catalytically dead INPP5E
    Depositor
    Olof Idevall-Hagren, Pietro De Camilli
    Article
    Idevall-Hagren et al Proc Natl Acad Sci U S A. 2012 Aug 28;10
    Insert
    INPP5E (INPP5E Human)
    Use
    Tags
    CRY2 (photolyase homology region domain of Arabid…
    Expression
    Mammalian
    Mutation
    C-terminal region, aa 214_644, C641A mutation to …
    Promoter
    CMV
    Available since
    July 11, 2016
    Availability
    Academic Institutions and Nonprofits only

  7. pUBQ10::ZF2CACTA1_3xFLAG_TET1cd

    Plasmid
    #106433
    Purpose
    pUBQ10 driven Zinc Finger human TET1 catalytic domain fusion that targets the CACTA1 promoter in arabidopsis
    Depositor
    Steven Jacobsen
    Article
    Gallego-Bartolome et al Proc Natl Acad Sci U S A. 2018 Feb 27;11
    Insert
    pUBQ10::ZF1CACTA1_3xFLAG_TET1cd (TET1 Human, Mustard Weed, Synthetic)
    Use
    Synthetic Biology
    Tags
    3xFLAG
    Expression
    Plant
    Mutation
    Promoter
    Available since
    March 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    106433-plasmid-map-sequence-id-205504

    Enlarge

  8. pDest-1.7kbfabp6-eGFP-pA-CrymCherry

    Plasmid
    #159088
    Purpose
    LR construct using backbone with Cry:mCherry insert to identify fish with transgene, and with p5E-1.7kbfabp6, pME-eGFP, and p3E-pA inserts
    Depositor
    John Rawls
    Article
    Wen et al Sci Adv. 2021 Jul 23;7(30). pii: 7/30/ea
    Inserts
    1.7kb fabp6 promoter (fabp6 Zebrafish)
    eGFP
    polyA
    Use
    Fluorescent reporter
    Tags
    Expression
    Mutation
    Promoter
    1.7kb fabp6
    Available since
    Sept. 29, 2022
    Availability
    Academic Institutions and Nonprofits only
    159088-plasmid-map-sequence-id-313129

    Enlarge

  9. mVenus-proα2(I)-G610C

    Plasmid
    #119839
    Purpose
    Expresses mouse Type I procollagen α2 chain (Col1a2) with Gly610Cys mutation and mVenus between signal sequence and exon 6
    Depositor
    Sergey Leikin
    Article
    Omari et al Proc Natl Acad Sci U S A. 2018 Oct 23;11
    Insert
    Type I procollagen α2 chain (Col1a2 Mouse)
    Use
    Tags
    mVenus
    Expression
    Mammalian
    Mutation
    Col1a2 exons 2-5 replaced by fluorescent tag, Gly…
    Promoter
    CMV
    Available since
    Jan. 28, 2019
    Availability
    Academic Institutions and Nonprofits only
    119839-plasmid-map-sequence-id-233845

    Enlarge

  16. pGL2-hOct4 DE-SV40-Luc

    Plasmid
    #26279
    Purpose
    Addgene next-generation sequencing identified numerous modifications from the indicated plasmid backbone. Please see the sequences link for additional information.
    Depositor
    Rudolf Jaenisch
    Article
    Hanna et al Proc Natl Acad Sci U S A. 2010 May 18. 1
    Insert
    Oct4 Distal Enhancer Element (DE) (POU5F1 Human)
    Use
    Luciferase
    Tags
    Expression
    Mammalian
    Mutation
    See Depositor Comments below
    Promoter
    Available since
    Oct. 18, 2010
    Availability
    Academic Institutions and Nonprofits only
    26279-plasmid-map-sequence-id-244682

    Enlarge

  17. mCerulean-proα2(I)-G610C

    Plasmid
    #119837
    Purpose
    Expresses mouse Type I procollagen α2 chain (Col1a2) with Gly610Cys mutation and mCerulean between signal sequence and exon 6
    Depositor
    Sergey Leikin
    Article
    Omari et al Proc Natl Acad Sci U S A. 2018 Oct 23;11
    Insert
    Type I procollagen α2 chain (Col1a2 Mouse)
    Use
    Tags
    mCerulean
    Expression
    Mammalian
    Mutation
    Col1a2 exons 2-5 replaced by fluorescent tag, Gly…
    Promoter
    CMV
    Available since
    Jan. 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    119837-plasmid-map-sequence-id-233867

    Enlarge

  18. pCJ199

    Plasmid
    #162672
    Purpose
    pET-21b(+) based plasmid for expression of the putative MHET hydrolase from Comamonas thiooxydans (Genbank WP_080747404.1) with C-terminal His tag, codon optimized for expression in E. coli K12.
    Depositor
    Gregg Beckham
    Article
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    Putative MHET hydrolase from Comamonas thiooxydans (Genbank WP_080747404.1) with signal peptide
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    codon optimized for expression in E. coli K12
    Promoter
    T7/lac
    Available since
    Jan. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    162672-plasmid-map-sequence-id-321466

    Enlarge

Showing: 5981 - 6000 of 6933 results