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N295A (EcN), Y160A (EcI) MetNIQ in pBAD

Plasmid

#118260

Purpose

N295A Mutation of MetN and Y160A in MetI in Methionine ABC Transporter for Transport Activity Assay

Depositor

Douglas Rees

Article

Nguyen et al Proc Natl Acad Sci U S A. 2018 Oct 23. p

Inserts

MetN (N295A) (metN E. coli)
MetI (Y160A) (metI E. coli)
MetQ (metQ E. coli)

Tags

10x HIs Tag, 6x His Tag, and Enterokinase Cleavag…

Expression

Bacterial

Mutation

Changed Glutamic Acid (Glu) 295 to Alanine (Ala) …

Promoter

None and araBAD Promoter

Available Since

Nov. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pDONR223_NFE2L2_p.G31V

Plasmid

#81518

Purpose

Gateway Donor vector containing NFE2L2 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

NFE2L2 (NFE2L2 Human)

Use

Gateway entry vector

Mutation

G31V

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_PIK3CA_p.E726K

Plasmid

#82845

Purpose

Gateway Donor vector containing PIK3CA, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

PIK3CA (PIK3CA Human)

Use

Gateway entry vector

Mutation

E726K

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_CDK4_p.A23S

Plasmid

#81327

Purpose

Gateway Donor vector containing CDK4 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

CDK4 (CDK4 Human)

Use

Gateway entry vector

Mutation

A23S

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_ERBB2IP_p.E463K

Plasmid

#81587

Purpose

Gateway Donor vector containing ERBB2IP , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

ERBB2IP (ERBIN Human)

Use

Gateway entry vector

Mutation

T271P; E463K; D547G; E1205G; Q1226P; K1287R

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_PTEN_p.H123Y

Plasmid

#82815

Purpose

Gateway Donor vector containing PTEN, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

PTEN (PTEN Human)

Use

Gateway entry vector

Mutation

H123Y

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_KEAP1_p.P278S

Plasmid

#82832

Purpose

Gateway Donor vector containing KEAP1, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

KEAP1 (KEAP1 Human)

Use

Gateway entry vector

Mutation

P278S, C624S

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_KEAP1_p.E117K

Plasmid

#82791

Purpose

Gateway Donor vector containing KEAP1, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

KEAP1 (KEAP1 Human)

Use

Gateway entry vector

Mutation

E117K; C624S

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_KEAP1_p.R601W

Plasmid

#82794

Purpose

Gateway Donor vector containing KEAP1, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

KEAP1 (KEAP1 Human)

Use

Gateway entry vector

Mutation

R601W; C624S

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_DCAF8_p.P156S

Plasmid

#82864

Purpose

Gateway Donor vector containing DCAF8, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

DCAF8 (DCAF8 Human)

Use

Gateway entry vector

Mutation

P156S

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_DCAF8_p.E63K

Plasmid

#82786

Purpose

Gateway Donor vector containing DCAF8, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

DCAF8 (DCAF8 Human)

Use

Gateway entry vector

Mutation

E63K

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_DCAF8_p.R559P

Plasmid

#82789

Purpose

Gateway Donor vector containing DCAF8, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

DCAF8 (DCAF8 Human)

Use

Gateway entry vector

Mutation

R559P

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_DCAF8_p.G588C

Plasmid

#82792

Purpose

Gateway Donor vector containing DCAF8, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

DCAF8 (DCAF8 Human)

Use

Gateway entry vector

Mutation

G588C

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_BECN1_WT

Plasmid

#82100

Purpose

Gateway Donor vector containing BECN1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

BECN1 (BECN1 Human)

Use

Gateway entry vector

Mutation

A103V

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_BRAF_p.G469S

Plasmid

#82838

Purpose

Gateway Donor vector containing BRAF, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

BRAF (BRAF Human)

Use

Gateway entry vector

Mutation

G469S

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_CHSY3_p.R350H

Plasmid

#81594

Purpose

Gateway Donor vector containing CHSY3 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

CHSY3 (CHSY3 Human)

Use

Gateway entry vector

Mutation

R350H

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_IRF4_p.K123R

Plasmid

#81427

Purpose

Gateway Donor vector containing IRF4 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

IRF4 (IRF4 Human)

Use

Gateway entry vector

Mutation

K123R

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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Anti-Kv2.1 K+ channel pS603 [L61/14R]

Plasmid

#140063

Purpose

Mammalian Expression Plasmid of anti-Kv2.1 K+ channel pS603 (Mouse). Derived from hybridoma L61/14.

Depositor

James Trimmer

Article

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-Kv2.1 K+ channel pS603 (Rat) recombinant mouse monoclonal antibody (Kcnb1 Mouse)

Expression

Mammalian

Promoter

dual CMV

Available Since

June 24, 2020

Availability

Academic Institutions and Nonprofits only

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Anti-RGS14 [N133/21.2R]

Plasmid

#114519

Purpose

Mammalian Expression Plasmid of anti-RGS14 (Rat). Derived from hybridoma N133/21.2.

Depositor

James Trimmer

Article

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-RGS14 (Rattus norvegicus) recombinant mouse monoclonal antibody (Rgs14 Mouse)

Expression

Mammalian

Promoter

dual CMV

Available Since

Feb. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR223_TP53_p.I195F

Plasmid

#81395

Purpose

Gateway Donor vector containing TP53 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

TP53 (TP53 Human)

Use

Gateway entry vector

Mutation

P72R; I195F; P278A

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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