We narrowed to 8,601 results for: Set

1. pSEED-ALFA:HA

   Plasmid

   #219646

   Purpose

   Permits GoldenGate assembly (BsmBI) of donor plasmids to knock-in ALFA and HA tags using the SEED/Harvest method

   Depositor

   Markus Affolter

   Article

   Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

   Insert

   ALFA:HA SEED Cassette
   

   Available Since

   Nov. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pALOD4

   Plasmid

   #111026

   Purpose

   Expresses domain 4 of anthrolysin O cholesterol sensor, has N-terminal His6-tag and two point mutations, S404C and C472A

   Depositor

   Arun Radhakrishnan

   Article

   Gay et al Biophys J. 2015 Mar 24;108(6):1459-69. d

   Insert

   Domain 4 of Anthrolysin O
   

   Tags

   His6 tag

   Expression

   Bacterial

   Mutation

   Mutations: S404C, C472A

   Promoter

   T7

   Available Since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pT2ST

   Plasmid

   #59384

   Purpose

   Template plasmid for amplification of a selection cassette that includes two transcription terminator elements, an I-SceI site and a trimethoprim resistance gene.

   Depositor

   Shelley Copley

   Article

   Kim et al BMC Biotechnol. 2014 Sep 25;14(1):84.

   Insert

   TT-ISceI-dfrA
   

   Use

   Template

   Available Since

   Sept. 29, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSP618

   Plasmid

   #169235

   Purpose

   Spear-ATAC lentiviral backbone - includes a mU6-sgRNA cassette with flanking Nextera sequencing adapters

   Depositor

   William Greenleaf

   Article

   Pierce et al Nat Commun. 2021 May 20;12(1):2969. doi:

   Insert

   mU6-sgRNA
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Aug. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. EC2_1_dCas9_sgRNA

   Plasmid

   #163710

   Purpose

   Yeast low copy plasmid with dCas9 and sgRNA expression cassette

   Depositor

   Yvonne Nygård

   Article

   Camara et al Sci Rep. 2020 Sep 3;10(1):14605. doi: 10

   Insert

   dCas9
   

   Expression

   Yeast

   Mutation

   Point mutations D10A and H840A

   Promoter

   ScTEF1

   Available Since

   Nov. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDZ415 (24MS2SL loxP-Kan-loxP)

   Plasmid

   #45162

   Depositor

   Robert Singer, Daniel Zenklusen

   Article

   Hocine et al Nat Methods. 2013 Feb;10(2):119-21. doi:

   Insert

   24xMS2 Stem Loop Cassette
   

   Available Since

   Aug. 8, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSEED-sfGFP:ALFA

   Plasmid

   #219642

   Purpose

   Permits GoldenGate assembly (BsmBI) of donor plasmids to knock-in sfGFP:ALFA using the SEED/Harvest method

   Depositor

   Markus Affolter

   Article

   Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

   Insert

   sfGFP:ALFA SEED Cassette
   

   Available Since

   Nov. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSEED-mGreenLantern:ALFA

   Plasmid

   #219644

   Purpose

   Permits GoldenGate assembly of donor plasmids (BsmBI) to knock-in mScarlet:ALFA using the SEED/Harvest method

   Depositor

   Markus Affolter

   Article

   Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

   Insert

   mGreenLantern:ALFA SEED Cassette
   

   Available Since

   Oct. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCRISPR-dCas9

   Plasmid

   #125687

   Purpose

   Gene knockdown for actinomycetes

   Depositor

   Tilmann Weber

   Article

   Tong et al ACS Synth Biol. 2015 Sep 18;4(9):1020-9.

   Insert

   streptomyces codon optimized spdCas9, sgRNA casette
   

   Use

   CRISPR and Synthetic Biology

   Promoter

   ermE*/tipA

   Available Since

   Feb. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBigT

    Plasmid

    #21270

    Purpose

    Used for making transgenic mice. Contains a loxP-flanked cassette with a PGK-neo selectable marker and a tpA transcriptional stop sequence.

    Depositor

    Frank Costantini

    Article

    Srinivas et al BMC Dev Biol. 2001;1:4. doi: 10.1186/147

    Type

    Empty backbone

    Use

    Mouse Targeting

    Available Since

    June 9, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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11. FDP-P10B2-A75-RZ-URA3

    Plasmid

    #130874

    Purpose

    Integration cassette for OrthoRep containing mKate driven by promoter 10B2 with a hardcoded polyA tail

    Depositor

    Chang Liu

    Article

    Zhong et al ACS Synth Biol. 2018 Dec 21;7(12):2930-2

    Insert

    mKate
    

    Use

    Expression of mkate, leu2(q180*), and ura3 on ort…

    Available Since

    Aug. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pARS3_MUbCAS9_GW

    Plasmid

    #128646

    Purpose

    T-DNA binary vector for CRISPR/Cas9 gene editing in rice; has a Gateway cassette for cloning gRNA

    Depositor

    G. Eric Schaller

    Article

    Worthen et al Development. 2019 Jun 3. pii: dev.174870

    Type

    Empty backbone

    Tags

    HA

    Expression

    Plant

    Available Since

    Jan. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. PCT62

    Plasmid

    #170391

    Purpose

    expresses three sgRNA cassettes

    Depositor

    Jonathan Weissman

    Article

    Quinn et al Science. 2021 Feb 26;371(6532). pii: sci

    Insert

    PCT62-targetsite
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Oct. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSEED-mGreenLantern

    Plasmid

    #219643

    Purpose

    Permits GoldenGate assembly of donor plasmids (BsmBI) to knock-in mGreenLantern using the SEED/Harvest method

    Depositor

    Markus Affolter

    Article

    Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

    Insert

    mGreenLantern SEED Cassette
    

    Available Since

    Nov. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAureus-TnCAM

    Plasmid

    #199807

    Purpose

    vector for transposome-mediated saturation mutagenesis and Tn-Seq in S. aureus

    Depositor

    Steve Salipante

    Article

    Lo et al Infect Immun. 2023 Oct 17;91(10):e002282

    Insert

    transposon cassette
    

    Expression

    Bacterial

    Available Since

    Oct. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRS-QUE7mu

    Plasmid

    #129311

    Purpose

    Low concentration ATP measurement

    Depositor

    Hiroyuki Noji

    Article

    Yaginuma et al Sci Rep. 2014 Oct 6;4:6522. doi: 10.1038

    Insert

    QUEEN-7mu
    

    Expression

    Bacterial

    Available Since

    Oct. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTKDP-neo

    Plasmid

    #71320

    Purpose

    Donor plasmid - kanamycin resistant donor cassette

    Depositor

    Thomas Kuhlman

    Article

    Tas et al PLoS One. 2015 Sep 2;10(9):e0136963. doi

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    Dec. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. YN2_1_LT58_X2site

    Plasmid

    #177705

    Purpose

    Expresses Cas9 and sgRNA cassette for targeting site X2 in yeast Saccharomyces cerevisiae

    Depositor

    Lisbeth Olsson

    Article

    Torello Pianale et al Front Microbiol. 2022 Jan 7;12:802169. d

    Insert

    Cas9
    

    Use

    CRISPR

    Expression

    Yeast

    Available Since

    Jan. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTmpKm

    Plasmid

    #176227

    Purpose

    A template plasmid for amplification of the cI-hok-neo cassette

    Depositor

    Sergey Sineoky

    Article

    Bubnov et al Nucleic Acids Res. 2022 Aug 26;50(15):89

    Insert

    cI of phage lambda, hok of the R1 plasmid, neo
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    Nov. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAC575

    Plasmid

    #127976

    Purpose

    AMA1 plasmid with ble selection marker, and USER cassette (PacI/Nt.BbvCI)

    Depositor

    Uffe Mortensen

    Article

    Vanegas et al Fungal Biol Biotechnol. 2019 May 1;6:6.

    Insert

    ble (bleomycin resistance marker)
    

    Use

    Fungal expression

    Expression

    Bacterial

    Promoter

    Aspergillus nidulans trpC promoter

    Available Since

    Aug. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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