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pDONR223 LC3B2 G120

Plasmid

#123077

Purpose

Gateway entry clone encoding human MAP1LC3B2 G120

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Microtubule-associated proteins 1A/1B light chain 3 beta 2 (MAP1LC3B2 Human)

Use

Gateway entry vector / entry clone

Mutation

Deleted amino acids 121-125. Stop codon after G12…

Available Since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR223 LC3B2 G120A

Plasmid

#123076

Purpose

Gateway entry clone encoding human MAP1LC3B2 G120A

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Microtubule-associated proteins 1A/1B light chain 3 beta 2 (MAP1LC3B2 Human)

Use

Gateway entry vector / entry clone

Mutation

Glycine 120 to Alanine

Available Since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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pJUMP18-cat_TU

Plasmid

#127051

Purpose

Basic Part Whole TU; Codes for chloramphenicol resistance; including promoter and terminator. Broad host range. From Bacillus/E.coli shuttle pSEVA3b61.

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Insert

Part Cat_TU

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

July 1, 2019

Availability

Academic Institutions and Nonprofits only

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pJUMP18-B0032-MV_RN

Plasmid

#127015

Purpose

Basic Part RN- RBS+N-terminus; B0033m Ribosome Binding Site + Met-Val N-terminus start.

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Insert

Part B0032m-MV_RN

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

July 1, 2019

Availability

Academic Institutions and Nonprofits only

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pJUMP19-min2

Plasmid

#126956

Purpose

"Minimal cargo" vector. Allows introducing any part using BsmBI; but doesn't have cloning reporter. Can be used to generate new promoter/terminator acceptor vectors.

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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gRNA[bTub].1026C

Plasmid

#112691

Purpose

express gRNA targeting bTub under dU6-3 promoter

Depositor

Omar Akbari

Article

Kandul et al Nat Commun. 2019 Jan 8;10(1):84. doi: 10

Insert

U6.3-gRNA[bTub]

Use

CRISPR

Promoter

dU6-3

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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RASL10A

Plasmid

#55674

Purpose

expression of mouse RASL10A

Depositor

Julian Downward

Article

Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

Insert

RASL10A (Rasl10a Mouse)

Expression

Bacterial

Promoter

lac

Available Since

Sept. 30, 2014

Availability

Academic Institutions and Nonprofits only
pDGB3_alpha1R

Plasmid

#68230

Purpose

GoldenBraid level 1 Alpha plant expression vector.

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

empty vector

Use

Synthetic Biology

Expression

Plant

Available Since

March 16, 2016

Availability

Academic Institutions and Nonprofits only

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pPromE8 (GB0914)

Plasmid

#68175

Purpose

Provides the S. Lycopersicum fruit promoter E8 as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

SlE8 promoter

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

March 16, 2016

Availability

Academic Institutions and Nonprofits only

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Pr-delRBS-deGFP

Plasmid

#67744

Purpose

Pr promoter expressing a deGFP that is missing a ribosomal binding site

Depositor

Richard Murray

Article

Siegal-Gaskins et al ACS Synth Biol. 2014 Jun 20;3(6):416-25.

Insert

deGFP-T500

Use

Synthetic Biology

Expression

Bacterial

Promoter

OR2-OR1-Pr

Available Since

Aug. 7, 2015

Availability

Academic Institutions and Nonprofits only

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pT35S (GB0036)

Plasmid

#68187

Purpose

Provides the CaMV 35S terminator as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

CaMV 35S terminator

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Oct. 22, 2015

Availability

Academic Institutions and Nonprofits only

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Myc-CICf_W37A

Plasmid

#48186

Purpose

Expresses W37A mutated Cic

Depositor

Huda Zoghbi

Article

Kim et al Genes Dev. 2013 Mar 15;27(6):590-5. doi:

Insert

Capicua (Cic Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 4, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA4 rat NZAP

Plasmid

#45908

Depositor

Harmit Malik

Article

Kerns et al PLoS Genet. 2008 Jan;4(1):e21. doi: 10.1

Insert

Zc3hav1 zinc finger CCCH type, antiviral 1 (Zc3hav1 Rat)

Tags

HA

Expression

Mammalian

Available Since

Nov. 4, 2013

Availability

Academic Institutions and Nonprofits only
pAc5.1B-lambdaN-HA-DmDCP2-V5His6

Plasmid

#22409

Depositor

Elisa Izaurralde

Article

Eulalio et al Mol Cell Biol. 2007 Jun . 27(11):3970-81

Insert

decapping protein 2, variant A (DCP2 Fly)

Use

Drosophila expression

Tags

V5-His6 and lambdaN-HA

Available Since

Nov. 5, 2009

Availability

Academic Institutions and Nonprofits only

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pDGB3_alpha2R

Plasmid

#68231

Purpose

GoldenBraid level 1 Alpha plant expression vector.

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

empty vector

Use

Synthetic Biology

Expression

Plant

Available Since

March 16, 2016

Availability

Academic Institutions and Nonprofits only

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XE151 XDpr-CS2+

Plasmid

#16771

Depositor

Randall Moon

Article

Cheyette et al Dev Cell. 2002 Apr. 2(4):449-61.

Insert

XDpr (dact1-b Frog)

Use

Xenopus expression

Available Since

March 13, 2008

Availability

Academic Institutions and Nonprofits only
pCMV-EPN-01*(ΔL1)

Plasmid

#80042

Purpose

Expression of PTAP L domain mutant EPN-01*

Depositor

Wesley Sundquist

Article

Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

Insert

pCMV-EPN-01*(ΔL2)

Tags

Myc

Expression

Mammalian

Mutation

PTAP sequence in p6 mutated to Ala

Promoter

CMV

Available Since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pENTR221-WNT3A

Plasmid

#79501

Purpose

MultiSite Gateway entry clones, second fragment (attL5, attL2) Human WNT3A CDS

Depositor

Peter Jon Nelson

Article

Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

Insert

WNT3A (WNT3A Human)

Use

Multisite gateway entry clone

Promoter

no Promoter

Available Since

May 12, 2017

Availability

Academic Institutions and Nonprofits only

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pCVD447

Plasmid

#78569

Purpose

E. coli E2348/69 LEE region (Type 3 Secretion System) cloned into pBluescriptKS.

Depositor

James Kaper

Article

Jarvis et al Proc Natl Acad Sci U S A. 1995 Aug 15;92

Insert

lee operon

Use

Cloning vector

Available Since

June 8, 2016

Availability

Academic Institutions and Nonprofits only
pP2A11 (GB0082)

Plasmid

#68168

Purpose

Provides the S. Lycopersicum fruit promoter 2A11 as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

Sl2A11 promoter

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Oct. 22, 2015

Availability

Academic Institutions and Nonprofits only

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