We narrowed to 7,105 results for: mme

1. pAL3

   Plasmid

   #114673

   Purpose

   T7 vector having very low basal expression of target protein can clone and express almost any gene. Asymmetric ligation clones as many as 3 genes simultaneously for co-expression from the same mRNA.

   Depositor

   William Studier

   Article

   Studier et al Curr Protoc Mol Biol. 2018 Oct;124(1):e6

   Type

   Empty backbone

   Use

   Tags

   optional Ser-Gly-6His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCDNA3-ySet2

   Plasmid

   #51698

   Purpose

   Expresses full length yeast Set 2 gene in a mamalian expression vector

   Depositor

   Tom Misteli

   Article

   Luco et al Science. 2010 Feb 19;327(5968):996-1000.

   Insert

   yeast Set2 (SET2 Budding Yeast)
   

   Use

   Tags

   C-terminal FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 16, 2014

   Availability

   Academic Institutions and Nonprofits only

3. pcDNA-NES-ZapCmR2

   Plasmid

   #59015

   Purpose

   Cytosolic zinc-binding FRET biosensor

   Depositor

   Amy Palmer

   Article

   Miranda et al PLoS One. 2012;7(11):e49371. doi: 10.137

   Insert

   NES-ZapCmR2 genetically encoded cytosolic Zn(II) sensor
   

   Use

   Tags

   Nuclear Exclusion Signal

   Expression

   Mammalian

   Mutation

   See comment

   Promoter

   CMV

   Available since

   Aug. 21, 2014

   Availability

   Academic Institutions and Nonprofits only

4. pcDNA-NLS-ZapCmR2

   Plasmid

   #59012

   Purpose

   Nuclear zinc-binding FRET biosensor

   Depositor

   Amy Palmer

   Article

   Miranda et al PLoS One. 2012;7(11):e49371. doi: 10.137

   Insert

   NLS-ZapCmR2 genetically encoded Zn(II) sensor
   

   Use

   Tags

   SV40 NLS

   Expression

   Mammalian

   Mutation

   See comment

   Promoter

   CMV

   Available since

   Aug. 21, 2014

   Availability

   Academic Institutions and Nonprofits only

5. pLJC6-NADK-3xFLAG

   Plasmid

   #163452

   Purpose

   lentiviral overexpression vector for human NADK

   Depositor

   Jason Cantor

   Article

   Rossiter et al Cell Metab. 2021 Feb 23. pii: S1550-4131

   Insert

   NADK (NADK Human)
   

   Use

   Lentiviral

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   See: comments

   Promoter

   Ubc

   Available since

   March 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA-NES-ZapCmR1.1

   Plasmid

   #59014

   Purpose

   Cytosolic zinc-binding FRET biosensor

   Depositor

   Amy Palmer

   Article

   Miranda et al PLoS One. 2012;7(11):e49371. doi: 10.137

   Insert

   NES-ZapCmR1.1 genetically encoded cytosolic Zn(II) sensor
   

   Use

   Tags

   Nuclear Exclusion Signal

   Expression

   Mammalian

   Mutation

   See comment

   Promoter

   CMV

   Available since

   Aug. 21, 2014

   Availability

   Academic Institutions and Nonprofits only

7. pcDNA3 Glt1a

   Plasmid

   #98705

   Purpose

   mammalian expression of Glt1a

   Depositor

   Paul Rosenberg

   Article

   Chen et al J Neurosci. 2002 Mar 15;22(6):2142-52.

   Insert

   Glt1a (Slc1a2 Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   I288V (please see depositor comments below)

   Promoter

   CMV

   Available since

   July 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. Plas-CRISPR_reporter

   Plasmid

   #157658

   Purpose

   CRISPR reporter for mRNA quantification, integrative plasmid into NPR2 gene

   Depositor

   Frank Albert

   Article

   Brion et al Elife. 2020 Nov 16;9. pii: 60645. doi: 1

   Insert

   dCAS9-VP64, mCherry, KanMX
   

   Use

   Insert storage (replicative in e. coli)

   Tags

   Expression

   Mutation

   N28D in mCherry- please see depositor comments be…

   Promoter

   Available since

   Sept. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLITMUS-rpoN-cI4A5C6G7G,P-J23106-geneIII

   Plasmid

   #80864

   Purpose

   Phagemid (encodes evolved cI4A5C6G7G,P and M13 gene III)

   Depositor

   Mark Isalan

   Article

   Brodel et al Nat Commun. 2016 Dec 16;7:13858. doi: 10

   Inserts

   M13 gene III
   cI4A5C6G7G,P
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   238G to 238D (please see depositor comment below)

   Promoter

   BBa_J23106 and rpoN

   Available since

   Dec. 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMD19T-psba1-Ppsba2-dCas9-SpR

    Plasmid

    #73220

    Purpose

    Contains dCas9 from S. pyogenes under constitutive promoter. Suicide vector inserts into psba1 site of Synechocystis. Carries spectinomycin resist. Recommend E. coli Copy cutter for propogation.

    Depositor

    Paul Hudson

    Article

    Yao et al ACS Synth Biol. 2015 Dec 28.

    Insert

    dCas9 from S. pyogenes
    

    Use

    Tags

    c-myc

    Expression

    Bacterial

    Mutation

    Silent mutation at bp 1341 A->C to remove an E…

    Promoter

    PpsbA2

    Available since

    March 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA-NLS-ZapCmR1.1

    Plasmid

    #59011

    Purpose

    Nuclear zinc-binding FRET biosensor

    Depositor

    Amy Palmer

    Article

    Miranda et al PLoS One. 2012;7(11):e49371. doi: 10.137

    Insert

    NLS-ZapCmR1.1 genetically encoded Zn(II) sensor
    

    Use

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    See comment

    Promoter

    CMV

    Available since

    Aug. 21, 2014

    Availability

    Academic Institutions and Nonprofits only

12. pcDNA3 Glt1b

    Plasmid

    #98706

    Purpose

    mammalian expression of Glt1b

    Depositor

    Paul Rosenberg

    Article

    Chen et al J Neurosci. 2002 Mar 15;22(6):2142-52.

    Insert

    Glt1b (Slc1a2 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    I348V and N365Y (please see depositor comments se…

    Promoter

    CMV

    Available since

    July 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. TA0474_pET21a(+)

    Plasmid

    #13839

    Depositor

    SeWon Suh

    Article

    Kang et al Acta Crystallograph Sect F Struct Biol C

    Insert

    IS200 transposase (Ta0474 Thermoplasma acidophilum)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    See Depositor Comments Below

    Promoter

    Available since

    June 29, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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14. pWPI-NP1

    Plasmid

    #65060

    Purpose

    Constitutive expression of NP1

    Depositor

    Ramon Trullas

    Article

    Figueiro-Silva et al J Neurosci. 2015 Apr 8;35(14):5504-21. d

    Insert

    Neuronal Pentraxin 1 (Nptx1 Rat)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    * see comments

    Promoter

    EF1alpha

    Available since

    July 29, 2015

    Availability

    Academic Institutions and Nonprofits only

15. pLITMUS-rpoN-cI4A5C6G7G-J23106-geneIII

    Plasmid

    #80909

    Purpose

    Phagemid (encodes cI4A5C6G7G and M13 gene III)

    Depositor

    Mark Isalan

    Article

    Brodel et al Nat Commun. 2016 Dec 16;7:13858. doi: 10

    Inserts

    M13 gene III
    cI4A5C6G7G
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    238G to 238D (please see depositor comment below)

    Promoter

    BBa_J23106 and rpoN

    Available since

    Dec. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. p180 pCIG-HDAC1 mut

    Plasmid

    #11053

    Depositor

    Ramesh Shivdasani

    Article

    Tou et al Mol Cell Biol. 2004 Apr . 24(8):3132-9.

    Insert

    HDAC1 (Hdac1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Deletion of aa 1-56: deacetylase domain deletion

    Promoter

    Available since

    Dec. 6, 2005

    Availability

    Academic Institutions and Nonprofits only

17. pK3FS-PYK1

    Plasmid

    #85777

    Purpose

    N-ICE plasmid pK3FS-PYK1

    Depositor

    Scott Briggs

    Article

    Zhang et al Yeast. 2016 Dec 12. doi: 10.1002/yea.322

    Insert

    loxP-KanMX-loxP-PYK1p-3FLAG
    

    Use

    Cre/Lox

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Jan. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pENTRdeltaGFPmirGE-DroshaT1.5.5

    Plasmid

    #61084

    Purpose

    pENTR plasmid containing a mutated GFP followed by 3 miRNA haipins: recommended as backbone.

    Depositor

    Patrick Salmon

    Article

    Myburgh et al Mol Ther Nucleic Acids. 2014 Oct 28;3:e2

    Insert

    deltaGFP
    

    Use

    RNAi

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. 4RG2

    Plasmid

    #64658

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    53BP1 (TP53BP1 Human)
    

    Use

    Tags

    6xHis-TEV and MHHHHHHSSGRENLYFQG

    Expression

    Bacterial

    Mutation

    See comments

    Promoter

    T7

    Available since

    April 27, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

20. pME-actn2

    Plasmid

    #109550

    Purpose

    Multisite gateway vector for 5' tagging with alpha actinin 2

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    alpha actinin 2
    

    Use

    Zebrafish plasmids

    Tags

    Expression

    Mutation

    Please see Depositor Comments

    Promoter

    Available since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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