We narrowed to 10,337 results for: plasmids 101

1. AAV-FLEX-mCherry-FHA1-subT391A-mCherry

   Plasmid

   #104462

   Purpose

   Cre-dependent, Non-phosphorylatable PKA consensus substrate tagged with mCherry

   Depositor

   Bernardo Sabatini

   Article

   Chen et al Neuron. 2017 Dec 6;96(5):1070-1083.e5. d

   Insert

   mCherry-subT391A-FHA1-mCherry
   

   Use

   AAV

   Tags

   mCherry

   Expression

   Mutation

   Promoter

   CAG

   Available Since

   June 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. PB-TAC-WTSI

   Plasmid

   #80486

   Purpose

   piggyBac transposon for dox-inducible expression of the WTSI polycistronic reprogramming cassette (with mCherry)

   Depositor

   Knut Woltjen

   Article

   Kim et al Stem Cell Reports. 2015 Apr 14;4(4):727-

   Insert

   WTSI
   

   Use

   Piggybac transposon

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   tetO

   Available Since

   Nov. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. FUP95Q15AGW (D5)

   Plasmid

   #74028

   Purpose

   lentiviral expression of Psd95-Q15A-EGFP fusion

   Depositor

   Robert Malenka, Weifeng Xu, Oliver Schluter

   Article

   Xu et al Neuron. 2008 Jan 24;57(2):248-62. doi: 1

   Insert

   Psd95 (DLG4 Rat)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Q15A

   Promoter

   hUbC

   Available Since

   April 11, 2016

   Availability

   Academic Institutions and Nonprofits only

4. FUP95E17RGW (D6)

   Plasmid

   #74029

   Purpose

   lentiviral expression of Psd95-E17R-EGFP fusion

   Depositor

   Robert Malenka, Weifeng Xu, Oliver Schluter

   Article

   Xu et al Neuron. 2008 Jan 24;57(2):248-62. doi: 1

   Insert

   Psd95 (DLG4 Rat)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   E17R

   Promoter

   hUbC

   Available Since

   March 31, 2016

   Availability

   Academic Institutions and Nonprofits only

5. pcDNA3.2 mir-1-1 reporter mir193b-wt

   Plasmid

   #46734

   Depositor

   David Bartel

   Article

   Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

   Insert

   mir193b-wt (MIR193B Synthetic, Human)
   

   Use

   RNAi

   Tags

   V5 and hsa-mir-1-1

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   June 29, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.2 mir-1-1 reporter dme-mir-279

   Plasmid

   #46668

   Depositor

   David Bartel

   Article

   Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

   Insert

   dme-mir-279
   

   Use

   RNAi

   Tags

   V5 and hsa-mir-1-1

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   July 31, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.2 mir-1-1 reporter dme-mir-92a

   Plasmid

   #46666

   Depositor

   David Bartel

   Article

   Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

   Insert

   dme-mir-92a
   

   Use

   RNAi

   Tags

   V5 and hsa-mir-1-1

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   July 31, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHAGE2-TetO-OCT4-KLF4-cMYC

   Plasmid

   #216175

   Purpose

   Generate lentiviruses encoding for OCT4, KLF4 and c-MYC (polycistronic vector); used in conjunction with SOX vectors for pluripotency reprogramming

   Depositor

   Ralf Jauch

   Article

   Ho et al Stem Cell Reports. 2025 Mar 11;20(3):102

   Insert

   OCT4 (POU5F1, KLF4, MYC Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   July 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-pCMV-dSaCas9-VP64-pU6-sgRNA

   Plasmid

   #158990

   Purpose

   Vector G encodes pAAV-pCMV-dSaCas9-VP64-spA-pU6-sgRNA (BsaI) transgenes for AAV packaging and expression of CRISPR activator in mammalian cells

   Depositor

   Chung Tin

   Article

   Lau et al Mol Ther Nucleic Acids. 2019 Jun 7;16:63

   Insert

   dSaCas9-VP64
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   pCMV

   Available Since

   Aug. 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHR_PGK_SNIPR_Hinge Notch SNIPR (Notch2 JMD)

    Plasmid

    #188375

    Purpose

    Lentiviral vector - constitutive expression of an anti-CD19 SNIPR with a CD8alpha-variant2-ECD, a Notch1-TMD, a Notch2-JMD, and a Gal4VP64 transcriptional factor

    Depositor

    Kole Roybal

    Article

    Zhu et al Cell. 2022 Apr 14;185(8):1431-1443.e16.

    Insert

    PGK_antiCD19_CD8alpha-variant2-ECD_Notch1-TMD_Notch2-JMD_Gal4VP64
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    March 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBR322-TIMER

    Plasmid

    #103056

    Purpose

    medium copy plasmid for expression of a variant of the fluorescent timer protein suitable for bacteria under the control of the constitutive ybaJp promoter (works in E. coli and Salmonella enterica)

    Depositor

    Dirk Bumann

    Article

    Claudi et al Cell. 2014 Aug 14;158(4):722-733. doi: 1

    Insert

    TIMER-bac
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    mutations: S4T and S179T

    Promoter

    constitutive ybaJp promoter

    Available Since

    Nov. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. 4xnrUAS:MLS-KillerRed in pTol2pA2-acrys-EGFP-

    Plasmid

    #115516

    Purpose

    Plasmid driving expression of membrane bound KillerRed fluorophore under UAS response element, with a green lens reporter

    Depositor

    Marco Morsch

    Article

    Formella et al Redox Biol. 2018 Aug 23;19:226-234. doi:

    Insert

    MLS-KillerRed
    

    Use

    Gateway cloning

    Tags

    MLS

    Expression

    Mutation

    Promoter

    UAS

    Available Since

    Oct. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-CAG-fDIO-oG-WPRE-SV40pA

    Plasmid

    #74291

    Purpose

    pAAV vector to express oG (optimized Glycoprotein) in a Flp-dependent manner

    Depositor

    Edward Callaway

    Article

    Kim et al Cell Rep. 2016 Apr 26;15(4):692-699. doi

    Insert

    oG (optimized Glycoprotein)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    chimeric glycoprotein

    Promoter

    CAG

    Available Since

    April 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCW57.1-Tet-UPF1WT (pRKB264)

    Plasmid

    #99146

    Purpose

    Inducible lentiviral construct to express FLAG-tagged UPF1wt

    Depositor

    Robert Bradley

    Article

    Feng et al Mol Cell. 2017 Jul 20;67(2):239-251.e6.

    Insert

    UPF1 (UPF1 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    First 127 codons are optimized to reduce GC conte…

    Promoter

    pTight

    Available Since

    Nov. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. AAV-hSyn-EGFP-8X2C (AAV GABA mAGNET)

    Plasmid

    #114214

    Purpose

    AAV vector that targets expression to GABAergic inhibitory neurons in rodent cortex with 98% selectivity

    Depositor

    Xue Han

    Article

    Keaveney et al Cell Rep. 2018 Jul 10;24(2):294-303. doi

    Insert

    Enhanced Green Fluorescent Protein
    

    Use

    AAV and Mouse Targeting

    Tags

    None

    Expression

    Mammalian

    Mutation

    None

    Promoter

    human synapsin (hSyn)

    Available Since

    Nov. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pScalps eSpCas9_Zsgreen gRNA scaffold

    Plasmid

    #188449

    Purpose

    Expression of eSpCas9 tagged with Zsgreen

    Depositor

    Ajit Chande

    Article

    Mishra et al Mol Ther Nucleic Acids. 2022 Mar 8;28:20

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1-alpha

    Available Since

    Jan. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-pMecp2-dSaCas9-VP64-pU6-sgRNA

    Plasmid

    #158972

    Purpose

    Vector C encodes pAAV-pMecp2-dSaCas9-VP64-spA-pU6-sgRNA (BsaI) transgenes for AAV packaging and expression of CRISPR activator in neurons

    Depositor

    Chung Tin

    Article

    Lau et al Mol Ther Nucleic Acids. 2019 Jun 7;16:63

    Insert

    dSaCas9-VP64
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pMecp2

    Available Since

    Sept. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV hSyn-fDIO-taCasp3-TEVp

    Plasmid

    #207997

    Purpose

    Inducing apoptotic ablation of neurons through a process reliant on Flp recombinase

    Depositor

    Kazunari Miyamichi

    Article

    Goto et al Neuron. 2025 Feb 17:S0896-6273(25)00041-

    Insert

    taCasp3-TEVp
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    hSyn promoter

    Available Since

    Feb. 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. Bxb1-GT_AIO-TetOn_Down-Tandem Donor

    Plasmid

    #229783

    Purpose

    Bxb1-GT donor plasmid with downstream tandem syntax for all-in-one doxycycline-inducible expression (empty)

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Insert

    LacZ; rtTA-T2A-mTagBFP2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE and CAG

    Available Since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. Bxb1-GT_AIO-TetOn_mScarlet_Down-Tandem

    Plasmid

    #229794

    Purpose

    Bxb1-GT donor plasmid with downstream tandem syntax for all-in-one doxycycline-inducible mScarlet expression

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Insert

    TRE-mScarlet; rtTA-T2A-mTagBFP2
    

    Use

    Synthetic Biology

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Promoter

    TRE and CAG

    Available Since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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