We narrowed to 20,285 results for: ACE;

1. pSin_EF1a_CreN59_nMag_NLS_P2A_mCh_IRES_PuroR

   Plasmid

   #176310

   Purpose

   Expresses CreN59-nMag-NLS with mCherry marker.

   Depositor

   Yingxiao Wang

   Article

   Allen et al ACS Synth Biol. 2019 Oct 18;8(10):2359-2

   Insert

   CreN59_nMag_NLS_P2A_mCherry_IRES_puroR
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   human EF1alpha

   Available Since

   Feb. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRSF-G1(7AW)RS

   Plasmid

   #207516

   Purpose

   tRNA synthetase/tRNA pair for the in vivo incorporation of 7-Aza-L-Tryptophan (7AW) into proteins in E. coli in response to the amber (TAG) codon

   Depositor

   Thomas Huber

   Article

   Abdelkader et al ACS Sens. 2023 Oct 27. doi: 10.1021/acss

   Insert

   tRNA synthetase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Oct. 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. MTK4_001

   Plasmid

   #123810

   Purpose

   Encodes Bovine Growth Hormone polyadenylation signal as a Type 4 part to be used in the MTK system

   Depositor

   Hana El-Samad

   Article

   Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

   Insert

   Bgh pA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. (133) pcDNA3.1-Nup62-Flag-EPEA

   Plasmid

   #160744

   Purpose

   Expresses the nucleoporin Nup62 with C-terminal Flag and EPEA tags. Proteins of interest can be inserted by replacing Nup62 using Sgfl + Sgsl restriction enzymes

   Depositor

   Christina Woo

   Article

   Ramirez et al ACS Chem Biol. 2020 Apr 17;15(4):1059-10

   Insert

   Nup62-Flag-EPEA
   

   Use

   Tags

   EPEA and Flag

   Expression

   Mammalian

   Mutation

   Promoter

   T7/CMV

   Available Since

   May 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. MTK1_022

   Plasmid

   #123684

   Purpose

   Encodes ConLS and the human A4 insulator as a Type 1 part to be used in the MTK system

   Depositor

   Hana El-Samad

   Article

   Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

   Insert

   Connector LS-A4 insulator
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. MTK1_024

   Plasmid

   #123686

   Purpose

   Encodes ConL2 and human C3 insulator as a Type 1 part to be used in the MTK system

   Depositor

   Hana El-Samad

   Article

   Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

   Insert

   Connector L2-C3 insulator
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. MTK1_023

   Plasmid

   #123685

   Purpose

   Encodes ConL1 and human B1 insulator as a Type 1 part to be used in the MTK system

   Depositor

   Hana El-Samad

   Article

   Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

   Insert

   Connector L1- B1 insulator
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA-DiLACCO1

   Plasmid

   #218824

   Purpose

   Mammalian expression of genetically encoded green fluorescent L-lactate control biosensor

   Depositor

   Robert Campbell

   Article

   Hario et al ACS Cent Sci. 2024 Jan 31;10(2):402-416.

   Insert

   DiLACCO1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAV126

   Plasmid

   #63192

   Purpose

   pRS426 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.

   Depositor

   Jef Boeke

   Article

   Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available Since

   Aug. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. MTK234_008

    Plasmid

    #123902

    Purpose

    Encodes the GFP dropout cassette for multi-TU destination vectors as a type 234 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    GFP dropout for multiTU destination vectors
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. cyt-GafD-mTurboID-V5

    Plasmid

    #184640

    Purpose

    Expresses V5-tagged GlycoID construct: GafD_short-miniTurboID in mammalian cytosol for O-GlcNAc interactome tagging

    Depositor

    Charlie Fehl

    Article

    Liu et al ACS Chem Biol. 2022 Jul 12. doi: 10.1021

    Insert

    cyt-GlycoID-V5
    

    Use

    Tags

    Nuclear export sequence and V5 tag

    Expression

    Mammalian

    Mutation

    GafD -> expressed aa22-178 ; BirA* -> expre…

    Promoter

    CMV

    Available Since

    July 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAGGS-synPCB2.1

    Plasmid

    #139478

    Purpose

    Expression vector for phycocyanobilin (PCB) synthesis in mammalian cells.

    Depositor

    Kazuhiro Aoki

    Article

    Uda et al ACS Chem Biol. 2020 Nov 20;15(11):2896-2

    Insert

    MTS-Fnr-Fd-P2A-MTS-PcyA-FLAG-P2A-MTS-HA-HO1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG promoter

    Available Since

    April 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJ204-loxP-EF1a-mCherry-NeoR-lox2272-BGHpA-siteA-HDR

    Plasmid

    #154826

    Purpose

    Landing pad for Cre/lox-based recombinase-mediated cassette exchange (RMCE) in CHO cells, donor plasmid for CRISPR/Cas9-mediated targeted integration in CHO cells (site A)

    Depositor

    Lise Marie Grav

    Article

    Sergeeva et al ACS Synth Biol. 2020 Aug 24. doi: 10.102

    Inserts

    mCherry
    NeoR
    ZsGreen1-DR
    

    Use

    CRISPR and Cre/Lox

    Tags

    PEST

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, EF1a, and SV40 early promoter

    Available Since

    Aug. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. NDV-HN-SpyTag

    Plasmid

    #160000

    Purpose

    Expresses Newcastle disease virus HN protein (47-575) with C-terminal SpyTag fusion in mammalian cells

    Depositor

    Mark Howarth

    Article

    Rahikainen et al Angew Chem Int Ed Engl. 2020 Sep 4. doi:

    Insert

    NDV-HN-SpyTag
    

    Use

    Tags

    His6-tag

    Expression

    Mammalian

    Mutation

    Promoter

    Chicken beta-actin promoter

    Available Since

    Nov. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. qTAG-AAVS1-PGK-Puro-moxGFP

    Plasmid

    #227275

    Purpose

    AAVS1 targeting donor for the insertion of Puro and a medium strength PGK expressing moxGFP. To be co-transfected with sgRNA plasmid px330-AAVS1 (Addgene #227272)

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    AAVS1 Homology Arms flanking a 2A-Puro-PGK-moxGFP cassette (AAVS1 Human)
    

    Use

    CRISPR; Donor template

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Promoterless

    Available Since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCgVchcrRNA

    Plasmid

    #203815

    Purpose

    Expression of CRISPR RNA, shuttle vector for Corynebacterium glutamicum / E. coli

    Depositor

    Sheng Yang

    Article

    Yang et al ACS Synth Biol. 2023 Jul 21;12(7):2198-2

    Insert

    CRISPR(BsaI)
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTarget

    Plasmid

    #186452

    Purpose

    used as source for acquisition of new spacers

    Depositor

    John van der Oost

    Article

    Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    March 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. TetO-FUW-Rfx4

    Plasmid

    #61544

    Purpose

    Expresses Rfx4 in mammalian cells

    Depositor

    Rudolf Jaenisch

    Article

    Cassady et al Stem Cell Reports. 2014 Nov 6. pii: S221

    Insert

    Rfx4 (Rfx4 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 16, 2015

    Availability

    Academic Institutions and Nonprofits only

19. pR-CyOFP1

    Plasmid

    #228604

    Purpose

    Mammalian expression of CyOFP1, for MuSIC barcode construction

    Depositor

    Marc Birtwistle

    Article

    Lu et al ACS Synth Biol. 2025 May 16;14(5):1533-1

    Insert

    CyOFP1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    We introduced some silent mutations to avoid esse…

    Promoter

    CMV

    Available Since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pTU1-B-SP44s-GUS

    Plasmid

    #172430

    Purpose

    Expresses Streptomyces codon-optimised beta-D-glucuronidase

    Depositor

    Paul Freemont, Simon Moore

    Article

    Moore et al ACS Synth Biol. 2021 Feb 19;10(2):402-41

    Insert

    beta-D-glucuronidase
    

    Use

    Synthetic Biology

    Tags

    None

    Expression

    Bacterial

    Mutation

    Promoter

    SP44s

    Available Since

    Aug. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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