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His-CL7_MBP_1xNLS_iGeoCas9(C1)_2xNLS(star)

Plasmid

#222990

Purpose

Bacterial expression of iGeoCas9(C1) construct

Depositor

Jennifer Doudna

Article

Chen et al bioRxiv [Preprint]. 2023 Nov 15:2023.11.

Insert

His-CL7_MBP_1xNLS_iGeoCas9(C1)_2xNLS(star)

Use

CRISPR

Tags

CL7, His, and MBP

Expression

Bacterial

Promoter

tac

Available Since

July 31, 2024

Availability

Academic Institutions and Nonprofits only

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pSCL452

Plasmid

#219502

Purpose

Golden Gate entry vector for library cloning of msd-sgRNA arrays: GAL7p-Csy4-PaqCI-PaqCI-GAL7t // SNR52p-msr-SUP4t

Depositor

Seth Shipman

Article

Gonzalez-Delgado et al Nat Chem Biol. 2024 Nov;20(11):1482-1492

Insert

GAL7p-Csy4-PaqCI-PaqCI-GAL7t // SNR52p-msr-SUP4t

Expression

Yeast

Promoter

GAL7

Available Since

May 31, 2024

Availability

Academic Institutions and Nonprofits only

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p931-SWITCH-OVER insert: EF1a-Blasti

Plasmid

#217890

Purpose

insert vector for Switch-OVER: Single loxP-STOP-EF1a-Blasti-mU6

Depositor

Ulrich Elling

Article

Chylinski et al Nat Commun. 2019 Nov 29;10(1):5454. doi:

Type

Empty backbone

Use

CRISPR, Cre/Lox, and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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p223-Pulse-Switch

Plasmid

#217889

Purpose

Lentiviral Pulse-Switch vector for sgRNA expression, Cas9 and sgRNA expression are mutual exclusive; blastiR

Depositor

Ulrich Elling

Article

Chylinski et al Nat Commun. 2019 Nov 29;10(1):5454. doi:

Type

Empty backbone

Use

CRISPR, Cre/Lox, and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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pDN0617 (pDEST-mEGFP C-term, NatR)

Plasmid

#210484

Purpose

pDEST vector to tag gene of interest with mEGFP on the C-terminus to perform localization assays in S. cerevisiae.

Depositor

Christian Landry

Article

Evans-Yamamoto et al Mol Biol Evol. 2023 Nov 18:msad246. doi:

Type

Empty backbone

Use

Synthetic Biology; Destination vector for gateway…

Tags

mEGFP

Expression

Yeast

Promoter

ADH1

Available Since

Dec. 22, 2023

Availability

Academic Institutions and Nonprofits only

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Hy_pRnrS eGFP SV40

Plasmid

#195062

Purpose

Expresses eGFP mRNA from the Drosophila RnrS promoter

Depositor

Jeremy Wilusz

Article

Fujiwara et al Mol Cell. 2023 Nov 14:S1097-2765(23)0090

Insert

eGFP

Expression

Insect

Promoter

RnrS

Available Since

Nov. 29, 2023

Availability

Academic Institutions and Nonprofits only

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Hy_pAna eGFP SV40

Plasmid

#195063

Purpose

Expresses eGFP mRNA from the Drosophila Ana promoter

Depositor

Jeremy Wilusz

Article

Fujiwara et al Mol Cell. 2023 Nov 14:S1097-2765(23)0090

Insert

eGFP

Expression

Insect

Promoter

Ana

Available Since

Nov. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pUC57-fsr1

Plasmid

#179866

Purpose

Encodes the polyketide synthase Fsr1 from Fusarium vanettenii codon optimized for expression in Saccharomyces cerevisiae

Depositor

Jens Laurids Sørensen

Article

Bejenari et al J Microbiol Biol Educ. 2023 Nov 27;24(3)

Insert

Fusarubin polyketide synthase

Use

Synthetic Biology

Mutation

Codon optimized for expression in Saccharomyces c…

Available Since

Nov. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pET-15b-aδNb072

Plasmid

#204627

Purpose

Expresses the anti-IgD nanobody 072 (aδNb072) in the bacterial periplasm. aδNb072 is an orthosteric inhibitor of human anti-Phl p 7 antibodies of clone 102.1F10.

Depositor

James McDonnell

Article

Vester et al Antibodies (Basel). 2023 Nov 16;12(4):75

Insert

aδNb072

Tags

His6 and TEV protease cleavage site

Expression

Bacterial

Promoter

T7

Available Since

Nov. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pNK005C_BattB_mkozak_STIM1_IRES_mCherry-H2A-P2A-PuroR

Plasmid

#200639

Purpose

Low STIM1 abundance recombination plasmid for Matreyek Bxb1(GT) landing pad in dual landing pad cells

Depositor

Kenneth Matreyek

Article

Roelle et al ACS Synth Biol. 2023 Nov 3. doi: 10.1021

Insert

STIM1 (STIM1 Human)

Expression

Mammalian

Available Since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3/HF-CDC50B

Plasmid

#209224

Purpose

Mammalian expression of CDC50B

Depositor

Hye-Won Shin

Article

Takatsu et al J Biol Chem. 2011 Nov 4;286(44):38159-38

Insert

CDC50B (TMEM30B Human)

Tags

HA

Expression

Mammalian

Available Since

Oct. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pCA31-pCS2-CMV-stdPCP-p65-HSF1

Plasmid

#199462

Purpose

Expression of stdPCP-PH in zebrafish embryos

Depositor

Baohui Chen

Article

Liang et al Nat Commun. 2022 Nov 28;13(1):7329. doi:

Insert

stdPCP-PH

Expression

Mammalian

Promoter

CMV

Available Since

May 4, 2023

Availability

Academic Institutions and Nonprofits only

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pCA30-pCS2-CMV-stdMCP-p65-HSF1

Plasmid

#199461

Purpose

Expression of stdMCP-PH in zebrafish embryos

Depositor

Baohui Chen

Article

Liang et al Nat Commun. 2022 Nov 28;13(1):7329. doi:

Insert

stdMCP-PH

Expression

Mammalian

Promoter

CMV

Available Since

May 4, 2023

Availability

Academic Institutions and Nonprofits only

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pACT2-μ4

Plasmid

#198176

Purpose

Expression of GAL4 transcriptional activation domain (AD)-AP-4 μ4 fusion protein in yeast (yeast two-hybrid assays)

Depositor

Juan Bonifacino

Article

Guo et al Dev Cell. 2013 Nov 11;27(3):353-66. doi:

Insert

AP-4 μ4

Tags

GAL4 transcriptional activation domain (AD) fragm…

Expression

Yeast

Promoter

ADH1

Available Since

April 18, 2023

Availability

Academic Institutions and Nonprofits only

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pGTag NGFR EGFP nLAP -2

Plasmid

#194507

Purpose

CRISPR/Cas9-mediated generic protein tagging

Depositor

Frank Schnütgen

Article

Seibert et al MethodsX. 2022 Nov 18;9:101929. doi: 10.

Insert

NGFR-P2A-nLAP(EGFP)

Use

Bacterial cloning vector

Available Since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmNot2-W556E-dsRNAres_U

Plasmid

#147702

Purpose

Insect Expression of DmNot2-W556E-dsRNAres

Depositor

Elisa Izaurralde

Article

Boland et al Nat Struct Mol Biol. 2013 Nov;20(11):128

Insert

DmNot2-W556E-dsRNAres (Rga Fly)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pLNHA-C1-HsNot2-del409-432_T

Plasmid

#147625

Purpose

Mammalian Expression of DmNot2-del409-432

Depositor

Elisa Izaurralde

Article

Boland et al Nat Struct Mol Biol. 2013 Nov;20(11):128

Insert

DmNot2-del409-432 (Rga Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsNot2-del344-380_T

Plasmid

#147629

Purpose

Mammalian Expression of DmNot2-del344-380

Depositor

Elisa Izaurralde

Article

Boland et al Nat Struct Mol Biol. 2013 Nov;20(11):128

Insert

DmNot2-del344-380 (Rga Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsNot2-del380-404_T

Plasmid

#147630

Purpose

Mammalian Expression of DmNot2-del380-404

Depositor

Elisa Izaurralde

Article

Boland et al Nat Struct Mol Biol. 2013 Nov;20(11):128

Insert

DmNot2-del380-404 (Rga Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsNot2-del409-432_T

Plasmid

#147631

Purpose

Mammalian Expression of DmNot2-del409-432

Depositor

Elisa Izaurralde

Article

Boland et al Nat Struct Mol Biol. 2013 Nov;20(11):128

Insert

DmNot2-del409-432 (Rga Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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